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SECTION – A
Q. 3. (1) The transfer of pollen grains with the help of water is called hydrophily. Plants
that are pollinated by water are called hydrophilous plants.
(2) Hydrophilous plants possess small, inconspicuous unisexual flowers. They lack
fragrance, nectar and bright colour.
(3) Pollen grains and other floral parts are protected from getting wet.
(4) Stigma is long and sticky. e.g. Zostera, Vallisneria, etc.
Q. 16. (1)
Klinefelter’s syndrome is a genetic disorder caused due to trisomy of X
chromosome caused due to non-disjunction of sex chromosomes which takes
place during gamete formation.
(2) Chromosomal complement of Klinefelter’s syndrome is 44+XXY, having a total
of 47 chromosomes.
(3) Symptoms of Klinefelter’s syndrome are as follows :
(i)
The individuals with Klinefelter’s syndrome are sterile, tall, thin and
eunuchoid with poorly developed sexual characteristics. They have
underdeveloped and small testis. Spermatogenesis does not take place.
(ii) They have subnormal intelligence and show partial mental retardation.
Q. 17. (1) Australopithecus shows ape-like characteristics such as :
(i) Larger jaws and teeth
(ii) Prognathous face
(iii) The chin was absent
(iv) Projecting eyebrow ridges
(v) Cranial capacity ranged from 450-600 c.c.
(2) Australopithecus shows the man-like characteristics as follows :
(i) Erect posture and walking.
(ii) The vertebral column had a distinct lumbar curve with broad basin-like
pelvic girdle.
(iii) Dentition with the parabolic dental arch.
(iv) A simian gap was absent.
Australopithecus is therefore, rightly described as a man with ape brain.
Q. 19. (a) (1) Gibberellins are growth promoting hormones and were isolated from fungus
Gibberella fujikuroi by Kurasawa.
(2) Rice plants when infected with this fungus show stem elongation i.e. Bakane
disease.
(3) Yabuta and Sumuki isolated gibberellins in crystalline form, from fungal
culture and named it gibberellins.
(4) Gibberellins are synthesized from mevalonic acid at young leaves, seeds, root
and stem tips and show non-polar transport.
(b) Function of Gibberellins : Increase in length of internodes thereby elongation of
stem.
Q. 20. (1) The blood cells shown in the diagram are white blood cells or leucocytes.
(2)
Cell A is neutrophil. These cells are phagocytic in function thus engulf
microorganisms.
Cell D is lymphocyte. Its function is to show immune response of body by
producing antibodies.
(3)
Cell D or lymphocyte is a large, round nucleus and agranular cytoplasm.
It originates in bone marrow, lymph nodes and thymus.
Q. 21. Endocrine cells of pancreas form groups of cells called Islets of Langerhans. There
are four kinds of cells in Islets of Langerhans which secrete hormones.
(1) Alpha (α ) cells : They are 20% and secrete glucagon. Glucagon is a hyperglycemic
hormone. it stimulates liver for glucogenolysis and increases the blood glucose
level.
(2) Beta (β) cells : They are 70% and secrete insulin. Insulin is a hypoglycemic
hormone. It stimulates liver and muscles for glycogenesis. This lowers blood
glucose level.
(3) Delta (δ) cells : They are 5% and secrete somatostatin. Somatostatin inhibits the
secretion of glucagon and insulin. It also decreases the gastric secretions, motility
and absorption in digestive tract. In general it is a growth inhibiting factor.
(4) PP cells or F cells : They form 5%. They secrete pancreatic polypeptide (PP)
which inhibits the release of pancreatic juice.
Q. 24. (1) Animals which are well-adapted to live in deserts are called xerocoles. These
animals show adaptations for water conservation or heat tolerance.
(2) These animals show low basal metabolic rate. They obtain moisture from succulent
plants and rarely drink water. E.g Gazella and Oryx.
(3) Desert animals like camel produce concentrated urine and dry dung.
(4) Many other hot desert animals are nocturnal, seeking out shade during the day or
dwelling underground in burrows.
(5) Smaller animals from desert, emerge from their burrows at night.
(6) Mammals living in cold deserts have developed greater insulation through warmer
body fur and insulating layers of fat beneath the skin.
6 SOLUTIONS TO NAVNEET PRACTICE PAPERS : STD. XII
Q. 25. (1) When there is a succession from algae to forest, it depends upon the amount of
water available.
(2) The succession begins with small phyto-planktons followed by submerged and
free floating and then rooted hydrophytes, sages, grasses and finally the trees.
(3) Similarly, there is also a transformation from a pool of water to swamp, then
marsh and then mesic which means neither too dry nor too wet conditions.
(4) Then small plants like mosses can inhabit followed by herbs, shrubs and then
trees. Such succession ultimately leads to a stable climax forest community.
Q. 26. In-situ conservation Ex-situ conservation
(1) In-situ conservation is a onsite (1) Ex-situ conservation is done outside
conservation. the habitat of plants and animals.
(2) Plant and animal species are conserved (2)
Plant and animal species are
in their natural habitat for protecting conserved in artificial or manmade
endangered species. place.
(3) It is done in natural environment. (3) It is done in manmade environment.
(4)
National parks, Sanctuaries, (4) Zoo, aquarium, seed banks are the
biosphere reserve, etc. are set up for examples of ex-situ conservation
in-situ conservation.
(5) It is a dynamic process. Cheap and (5) It is static process. It is an expensive
convenient to conduct. and commercial process.
(6) Captive breeding is not successful in (6)
Captive breeding is successful and
all cases of in-situ conservation can help in increasing the number of
method. endangered organisms.
SECTION – D
Q. 27. (1) Isolation of DNA (gene) from the donor organism :
(a) From the cells of the donor organism DNA is isolated and purified. Then
cleaved using Restriction Endonucleases. This forms foreign DNA or
passenger DNA.
(b) A desired gene can also be obtained directly from genomic library or c-DNA
library.
(2) Insertion of desired foreign gene into a cloning vector (vehicle DNA) :
(a) The foreign DNA or passenger DNA is inserted into a cloning vector (vehicle
DNA)
(b) Plasmids are isolated from the bacteria and are cleaved by using same RE
which is used in the isolation of the desired gene from the donor.
(c) Enzyme DNA ligase is used to join foreign DNA and the plasmid DNA.
Q. 28. (1) Cerebrum is the largest part of the brain. It forms 80–85% volume of the brain.
(2) A median longitudinal fissure divides the cerebrum into two cerebral hemispheres.
These hemispheres are interconnected by a thick band of transverse nerve fibres
called corpus callosum.
(3) The outer part of cerebrum is called cerebral cortex while the inner part is called
cerebral medulla.
(4) The roof of cerebrum is called pallium. Pallium is highly folded forming sulci
and gyri. Sulci are depressions while gyri are ridges. The gyri increase the surface
area of cerebral cortex.
(5) The ventro-lateral walls of cerebrum are thickened and are called corpora striata.
(6) The cerebral cortex has three deep sulci, the central, lateral and parieto-occipital.
(7) These sulci divide the cerebral hemisphere into four lobes. These are frontal,
parietal, occipital and temporal lobes. A fifth median lobe called insula or insular
cortex is folded deep within the lateral sulcus.
(8) The central sulcus separates frontal and parietal lobes, the lateral sulcus separates
parietal and temporal lobes and the parietooccipital sulcus separates parietal and
occipital lobes.
1. Right atrium :
(1) Atria are thin walled receiving chambers on the upper side, these are separated
by interatrial septum.
(2) The right atrium receives deoxygenated blood from upper part of body through
superior vena cava and from the lower part of the body by inferior vena cava.
In the right atrium opens the coronary sinus which brings blood from the heart
muscles.
(3) Eustachian valve guards the opening of inferior vena cava while opening of
coronary sinus is guarded by Thebesian valve.
(4) An oval depression called fossa ovalis is present which represents the foramen
ovale of embryonic condition.
(5) Right atrium opens into the right ventricle.
2. Left atrium :
(1) The oxygenated blood from the lungs is brought into left atrium through four
openings of pulmonary veins.
(2) Left atrium opens into the left ventricle.
Ventricles :
(1) Two thick walled distributing chambers situated on the lower side of the heart.
Interventricular septum divides two ventricles.
(2) Left ventricle has thickest wall as it pumps blood to all parts of the body.
(3) The inner surface of the ventricle is thrown into a series of irregular muscular
ridges called columnae carnae or trabeculae carnae.
(4) Each atrium opens into the ventricle of its side through atrioventricular aperture.
These apertures are guarded by right tricuspid valve and left bicuspid valves
respectively.
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SECTION – A
Q. 5.
Functional genomics is a branch of genomics that involves the study of functions of
all gene sequences and their expressions in organisms.
Q. 7. Column A Column B
(1) Shedding of leaves Abscissic acid
(2) Induce flowering in LDP GA
(3) Apical dominance IAA
(4) Induce RNA synthesis Cytokinin
Q. 13. (a)
The climax community remains stable as long as the environment remains
unchanged.
(b)
Small phytoplankton are the pioneers in the aquatic habitat during primary
succession.
SECTION – C
Q. 15. (1)
Unisexuality, dichogamy, prepotency, heteromorphy and herkogamy are the
outbreeding devices.
(2) Unisexuality : The plants bear either male or female flowers. Due to unisexual
nature, self-pollination is avoided. Plants are either dioecious, e.g. Papaya or
monoecious, e.g. maize.
Apogamy Apospory
Embryo from Embryo from other Unreduced embryo sac Nucellar cells
egg haploid cells from megaspore mother form embryo sac
cell
Parthenogenesis Sterile plants
Diplospory Apomictic
E.g. Nicotiana embryo sac
E.g. Texacum
Q. 29. (1) The heart is hollow, muscular, conical organ about the size of one’s fist with
broad base and narrow apex tilted towards left measuring about 12 cm in length,
9 cm in breadth and weighing about 250 to 300 grams.
(2) The human heart has four chambers, two atria which are superior, small, thin
walled receiving chambers and two ventricles which are inferior, large, thick
walled, distributing chambers.
(3) Externally there is a transverse groove between the atria and the ventricles which
is known as atrioventricular groove or coronary sulcus.
(4) Between the right and left ventricles there is interventricular sulcus (pl. sulci).In
these sulci the coronary arteries and coronary veins are present.
(5) Oxygenated blood to the heart is supplied by coronary arteries while coronary
veins collect deoxygenated blood from the heart. The coronary veins join to form
coronary sinus which opens into the right atrium.
(6) Right atrium is larger in size than the left atrium. Deoxygenated blood from all
over the body is brought through superior vena cava and inferior vena cava and
poured into right atrium. Oxygenated blood from lungs is brought to heart by two
pairs of pulmonary veins which carry it to the left atrium.
(7) Pulmonary trunk is seen arising from the right ventricle, which carries deoxygenated
blood to lungs. While systemic aorta arises from the left ventricle and carries
oxygenated blood to all parts of the body.
(8) The pulmonary trunk and systemic aorta are connected by ligamentum arteriosum
that represents remnant of ductus arteriosus of foetus.
————
SECTION – A
Q. 1. (i) (d) Synergids
(ii) (c) Heart
(iii) (a) Monascus purpureus
(iv) (a) Spermatids
(v) (d) Abscissic acid
(vi) (b) cyclosis
(vii) (a) Primary
(viii) (b) Saccharomyces
(ix) (a) Himgiri
(x) (b) antimalarial
Q. 2. (i) Genetic code is degenerate as 61 codons code for 20 amino acids, that is two or
more codons can specify the same amino acid. e.g. Cysteine has two codons,
while Isoleucine has three codons.
(ii) There are 3 structural genes in the sequence lac-Z, lac-Y and lac-A.
(iii)
Water held tightly around soil particles, adsorbed or adhered water on fine
particles is called hygroscopic water.
(iv) Brown rust of wheat, Late blight of potato, Red rot of sugar cane, Smut of wheat,
(v) During DNA replication RNA primer provides 3ʹ OH to which DNA polymerase
enzyme can add nucleotides to synthesize new strand using parental strand of
DNA as template.
(vi) The individual transitional communities formed during succession are termed
seral communities.
(vii) The roof of diencephalon is epithalamus and floor of diencephalon is hypothalamus.
(viii) EW category means those living beings which are extinct in wild.
SECTION – B
Q. 7.
Human female is referred to as carrier of colour blindness because of the following
reasons :
(1) Females possess double X-chromosomes in her gametes.
(2) If one X-chromosome is carrying recessive gene for colour blindness, her other
dominant X hides the expression of colour blindness and hence she does not
become a patient.
(3) But such female can carry the defective gene to her progeny. Thus she is called
carrier of colour-blindness.
(4) A female having one recessive gene on X-chromosome is a carrier female, while
a female possessing both recessive genes on both the X-chromosomes will be
colour blind which is very rare.
Q. 8. (1) Ramapithecus was on direct line of evolution of man and was called an ape-man
like primate.
(2) Its fossils were obtained in the form of teeth and jaw bones in the rocks of Siwalik
Hills in India by Lewis and also in Kenya.
(3) It existed during late Miocene and early Pliocene epoch about 14 to 12 million
years ago.
(4) It walked erect on its hind limb.
Q. 9. (1) Root is the water absorbing organ of the plant. Root system helps in the absorption
of water and minerals.
(2) Terrestrial plants absorb liquid water from soil with root hairs while epiphytes like
orchids, have special hygroscopic tissue velamen that absorbs water vapour from
atmosphere.
2 SOLUTIONS TO NAVNEET PRACTICE PAPERS : STD. XII
(3) There are four zones in the root. Root cap is situated at tip of the root. Behind the
root caps is (a) zone of meristematic region, (b) zone of elongation, (c) zone of
absorption or root hair zone and (d) zone of maturation in the sequence.
Q. 10. (1) Mechanical means : Cervical caps, Diaphragms
(2) Chemical means : Foams, Jellies
(3) Intra-uterine devices : Lippe’s loop, Multiload 375
Q. 11. (1) Tidal Volume (TV) : It is the volume of air inspired or expired during normal
breathing. It is 500 ml.
(2) Inspiratory reserve volume (IRV) : The maximum or the extra volume of air
that is inspired during forced breathing in addition to TV (2000 to 3000 ml).
Q. 12. Pharynx and Larynx.
Pharynx Larynx
1. Pharynx is a short, vertical tube. 1.
Larynx is a sound producing organ
located at the end of pharynx.
2. Mouth leads to the pharynx. 2. Larynx leads to the oesophagus.
3. Vocal cords are absent. 3. Vocal cords are present.
4. Pharynx does not increase in size at the 4. Larynx increases in size at the time of
time of puberty. puberty.
5. Pharynx does not show Adam’s apple. 5. Larynx shows Adam’s apple in adult
males.
Q. 13. (1) In toxicology field, transgenic animals are used for detection of toxicants. They
are used as responsive test animals.
(2) They are used to evaluate a specific genetic change in molecular biology studies.
(3) In pharmaceutical industry, transgenic animals are used for targeted production
of pharmaceutical proteins, drug production and product efficacy testing.
(4) They are also used in study of mammalian developmental genetics.
Q. 14. (a) Succession is a spatial pattern which occurs over the time.
(b) Pioneer species are the species which occupy the ecosystem in the initial times.
(3) The wall of the anther can be differentiated into four layers, viz., epidermis,
endothecium, middle layers and tapetum.
(4) The epidermis is the outermost layer of the anther wall. It is made up of flattened
cells which are protective in function.
(5) The endothecium lies internal to the epidermis. It is made up of a single layer of
cells. The cells of endothecium show fibrous thickenings on radial walls.
(6) Internal to the endothecium, lie 1 to 3 layers of parenchymatous cells forming
middle layers of the anther wall. The cells of middle layers degenerate at maturity
during the formation of microspores.
SECTION – D
Q. 27. 1. Chromosomes are classified into the following four types according to the position
of the centromere in them :
Metacentric
Sub-metacentric
Acrocentric
Telocentric
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SECTION – A
Q. 1. (i) (a) Biotic community
(ii) (a) Down’s syndrome = 44 XY
(iii) (c) Heterochromatin has 2 to 3 times more DNA than in the euchromatin.
(iv) (a) Prosimi
(v) (a) Avena curvature test
(vi) (a) P
(vii) (c) Insulin-Hyperglycaemia
(viii) (d) Interfascicular cambium
(ix) (c) Climax community
(x) (c) ovulation
Q. 2. (i) (1) IVF : In Vitro Fertilization.
(2) AI : Artificial Insemination
(ii) During replication of DNA, SSBP proteins remain attached to both the separated
strands and prevent them from coiling back.
(iii) In bioassay of cytokinins, chlorophyll retention test is used.
(iv) Hypersecretion of growth hormones in adults causes abnormal elongation of long
bones of arms and legs and of lower jaw i.e. acromegaly
(v) Ganga-3 (maize), CO-12 (Jowar), Niphad (Bajra)
(vi) Transfection is the insertion of a vector into the eukaryotic cells whereas
transduction is inserting a viral vector in cloning procedures.
(vii) (1) Tissue culture (2) In vitro fertilization of eggs (3) Cryopreservation.
(viii) Bioethics is the study of moral vision, decision and policies of human behaviour
in relation to biological phenomena or events.
SECTION – B
Q. 3. (1) Polyembryony is the development of more than one embryo inside the seed.
(2) When such polyembryonic seeds germinate then multiple seedlings are produced
for increasing the chances of survival of new plants.
(3) Nucellar embryos are genetically identical to parent plants hence uniform plants
are obtained.
(4) In horticulture these are used as rootstock for grafting, hence they have significant
role in fruit breeding programmes e.g. Citrus, Mango.
SOLUTIONS TO NAVNEET PRACTICE PAPERS : STD. XII 1
Q. 4. Morula Blastula
1. Morula is the embryonic stage formed 1. Blastula is the embryonic stage formed
after the completion of cleavage. after the completion of blastulation.
2. Morula is formed 4 to 6 days after the 2. Blastula is formed 6 to 7 days after the
fertilization. fertilization.
3. Morula consists of 16 cells. 3. Blastula consists of more than 64 cells.
4. Morula is solid ball of cells. 4. Blastula is a hollow ball of cells.
Q. 5. Homozygous Heterozygous
1. Individuals with similar gene pairs are 1.
Individuals with different gene pairs
called homozygous. are called heterozygous.
2.
Homozygous individuals form only 2.
Heterozygous individuals form more
one type of gametes. than one type of gametes.
3. Individuals with similar gene pairs TT, 3. Individuals with dissimilar gene pairs
tt, RR and rr are homozygous. Tt and Rr are heterozygous.
4.
Homozygous are also called pure 4.
Heterozygous are referred to as
breed. hybrids.
(1) Mutualism Lichen having alga and fungus, Bumblebee and flowering
plant.
(2) Commensalism Epiphytic orchid and mango tree, Egret and cattle.
Q. 17. (1) Statement of Law of Dominance : When two homozygous individuals with one
or more sets of contrasting characters are crossed, the alleles that appear in F1 are
dominant and those which do not appear in F1 are recessive.
(2) Statement of Law of Segregation or Law of purity of gametes : When F1
hybrid forms gametes, the alleles segregate from each other and enter in different
gametes. The gametes formed are pure because they carry only one either dominant
allele or recessive allele each. Due to this the law is also called “Law of purity
of gametes”.
(3) Statement of Law of Independent Assortment : When hybrid possessing two
(or more) pairs of contrasting alleles forms gametes, these alleles in each pair
segregate independently of the other pair.
(1) Scientists have tried to establish relationship between species diversity and the
size of the habitat. It is considered that number of species present is directly
proportional to the area.
SECTION – D
Q. 27. (1) Parturition is the act of expelling out the mature foetus from the uterus of mother
via the vagina.
(2) When the foetus is fully mature, it starts secreting ACTH (Adreno Cortico Trophic
Hormone) from its pituitary.
(3) ACTH stimulates adrenal glands of foetus to produce corticosteroids.
(4)
These corticosteroids diffuse from foetal blood to mother’s blood across the
placenta. Corticosteroids accumulate in mother’s blood that results in decreased
amount of progesterone. Corticosteroids also increase secretion of prostaglandins.
(5) Simultaneously estrogen levels rise bringing about initation of contractions of
uterine muscular wall.
(6)
Reduced progesterone level and increased estrogen level cause secretion of
oxytocin from mother’s pituitary. This causes greater stimulation of myometrium
of uterus.
(7) Prostaglandins cause increased forceful contraction of uterus which expels the
foetus out of the uterus.
(8) Hormone relaxin secreted by the placenta makes the pubic ligaments and sacroiliac
joints of the mother loosen. This causes widening of birth canal which facilitates
the normal birth of the baby.
————
SECTION – A
Q. 1. (i) (c) Hypohydrophilous
(ii) (b) ampulla
(iii) (a) complete sex linkage
(iv) (d) Uracil
(v) (a) Pithecanthropus
(vi) (c) Random mating
(vii) (b) gravitational
(viii) (b) leaf fall and dormancy
(ix) (b) palatine
(x) (c) Streptomyces venezuelae
Q. 2. (i) Condoms in male, cervical caps and vaults in females.
(ii)
Two molecules each of histone proteins, viz. H2A, H2B, H3 and H4 are the
nucleosomal 'core' histones.
(iii) Gamete mortality, zygote mortality are the two types of post-zygotic isolating
mechanisms.
(iv)
Root hairs are ephemeral structures in the roots.
(v)
The layer of squamous epithelium lining the alveolus, basement membrane and
a layer of squamous epithelium lining the capillary wall help in gaseous exchange
between the alveolar air and the blood.
(vi)
The soil characteristics along with pH, mineral composition and topography, and
climatic factors determine the vegetation of an area.
(vii) Lice, mosquito feeding on human blood and ticks parasitic on dogs.
(viii)
India has three of world’s biodiversity, viz. Western Ghats, Indo-Burma and
eastern Himalayas.
SECTION – B
Q. 3. (1) Microsporogenesis : The process in which each microspore mother cell divides
meiotically to form tetrad of haploid microspores (pollen grains).
Megasporogenesis : It is the process of formation of haploid megaspores from
(2)
diploid megaspore mother cell.
SECTION – C
Q. 15. (1) Apomixis : The phenomenon of formation of embryo(s) by asexual methods
without formation of gametes and fertilization is termed as apomixis.
(2) There are three main categories of apomixis. (a) Recurrent (b) Non-recurrent and
(c) Adventive embryony.
(a) Recurrent apomixis : in this diploid sporophytic cell, archesporial cell or
nucellus form embryos, when diploid megaspore mother cell forms embryo
sac it is known as diplospory. It is also called apospory.
Q. 24. (a) The job of t-RNA is to pick up amino acids and transport them to ribosomes.
t-RNA is an adapter molecule. It reads the codons of m-RNA and also
simultaneously transfer specific amino acid to m-RNA ribosome complex. It
binds with amino acid at its 3’ end,
(b) In prokaryotes, translation can start before transcription is complete, as both these
processes occur in the same compartment, i.e. cytoplasm. But in eukaryotes,
transcription and processing of hnRNA occurs in nucleus. hnRNA then comes out
of the nucleus through nuclear pores and then it is translated at ribosomes in the
cytoplasm.
(c) (1) Translation is the process in which sequence of codons of m-RNA is decoded
and accordingly amino acids are added in specific sequence to form a
polypeptide on ribosomes.
SOLUTIONS TO NAVNEET PRACTICE PAPERS : STD. XII 7
(2) Ribosome has one binding site for m-RNA. It orients m-RNA molecule in
such a way that all the codons are properly read.
(3) Ribosome has three binding sites for t-RNA : P-site (peptidyl t-RNAsite),
A-site (aminoacyl t-RNA-site) and E-site (exit site).
(4) t-RNAs place the required amino acids in correct sequence and translate the
coded message of RNA.
(5) In eukaryotes, a groove which is present between two subunits of ribosomes,
protects the polypeptide chain from the action of cellular enzymes and also
protects m-RNA from the action of nucleases.
(6) Thus ribosome plays an important role in translation.
Q. 25. Behavioural adaptation in animals :
(1) Behavioural responses to cope with variations in their environment are shown by
few animals.
(2) Desert lizards manage to keep their body temperature fairly constant by behavioural
adaptations. They bask in the sun and absorb heat, when their body temperature
drops below the comfort zone, but move into shade, when the ambient temperature
starts increasing. Even snakes also show basking during winter months.
(3) Since they are ectothermic, this kind of behaviour saves them from extreme
temperatures.
(4) Many smaller animals show burrowing behaviour to adapt to the temperature
extremes.
(5) Some species burrow into the sand to hide and escape from the heat.
(6) Migrations shown by the birds and mammals are also behavioural responses for
adapting to severe winter temperatures.
Q. 26. Succession in aquatic habitat :
(1)
In aquatic habitats the pioneer species in primary succession are the small
phytoplankton.
(2) Phytoplankton are replaced by rooted-submerged plants (e.g. Hydrilla), rooted-
floating angiosperms (e.g. Lotus) followed by free-floating plants (e.g.Pistia),
then reed swamp (e.g. Typha), marsh-meadow (e.g. Cyperus), scrub (e.g. Alnus)
and finally the trees (e.g. Quercus) in a very systematic and gradual way.
(3) The climax again would be formation of forest. With passage of time, the water
body is converted into land.
(1) The DNA segment and excess of two primer molecules, four types of dNTPs, the
thermostable DNA polymerase are mixed together in ‘eppendorf tube’.
(2) One PCR cycle is of 3 – 4 minutes duration and it involves following steps :
Denaturation : The reaction mixture is heated at 90° – 98°C. Due to this
(a)
hydrogen bonds in the DNA break and two strands of DNA separate. This is
called denaturation.
(b) Annealing of primer : When the reaction mixture is cooled to 40° – 60°C,
the primer pairs with its complementary sequences in ssDNA. This is called
annealing.
(c) Extension of primer : In this step, the temperature is increased to 70° – 75°C.
At this temperature thermostable Taq DNA polymerase adds nucleotides to
3’end of primer using single-stranded DNA as template. This is called primer
extension. Duration of this step is about two minutes.
(6) On the outer surface current flows from site B to site A to complete the circuit
of current flow. Hence, the polarity at the site is reversed, and an action potential
is generated at site B. Thus, the impulse (action potential) generated at site A
arrives at site B.
(7) The sequence is repeated along the length of the axon and consequently the
impulse is conducted.
(8) The rise in the stimulus-induced permeability to Na+ is extremely short-lived. It
is quickly followed by a rise in permeability to K+.
(9) Within a fraction of a second, K+ diffuses outside the membrane and restores the
resting potential of the membrane at the site of excitation and the fibre becomes
once more responsive to further stimulation.
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