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    Flow cytometry is a technique used to analyze cells as they flow in a liquid stream through a machine. Key components include a flow cell to move cells through the machine, a laser to scatter light off the cells, and photodetectors to detect the scattered light. This allows cells to be analyzed by size, granularity, and surface proteins by using fluorescent antibodies. Flow cytometry has clinical uses such as detecting fetal red blood cells in a pregnant woman's bloodstream and identifying blood cells lacking proteins in paroxysmal nocturnal hemoglobinuria.

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    Flow Cytometry atf

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    Flow cytometry is a technique used to analyze cells as they flow in a liquid stream through a machine. Key components include a flow cell to move cells through the machine, a laser to scatter light off the cells, and photodetectors to detect the scattered light. This allows cells to be analyzed by size, granularity, and surface proteins by using fluorescent antibodies. Flow cytometry has clinical uses such as detecting fetal red blood cells in a pregnant woman's bloodstream and identifying blood cells lacking proteins in paroxysmal nocturnal hemoglobinuria.

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    © All Rights Reserved
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    4 views10 pages

    Flow Cytometry Atf

    Uploaded by

    nitish
    Flow cytometry is a technique used to analyze cells as they flow in a liquid stream through a machine. Key components include a flow cell to move cells through the machine, a laser to scatter light off the cells, and photodetectors to detect the scattered light. This allows cells to be analyzed by size, granularity, and surface proteins by using fluorescent antibodies. Flow cytometry has clinical uses such as detecting fetal red blood cells in a pregnant woman's bloodstream and identifying blood cells lacking proteins in paroxysmal nocturnal hemoglobinuria.

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    AfraTafreeh.

    com

    Flow Cytometry
    Jason Ryan, MD, MPH
    Flow Cytometry
    • Flow = motion of fluid
    • Cytometry = measurement of cells
    • Flow cytometry = Analysis of cells as they flow in a
    liquid through a narrow steam
    • Key point: Used to analyze cells
    AfraTafreeh.com

    • By size
    • By surface proteins
    AfraTafreeh.com

    Flow Cytometer
    • Key components:
    • Flow cell: moves cells through machine
    • Laser: light scattered by cells
    • Photodetector: detects light scatter

    Biol/Wikipedia
    Flow Cytometer

    AfraTafreeh.com

    Light Source
    Photodetector
    AfraTafreeh.com

    Flow Cytometer

    Light Forward Scatter


    Size

    Side Scatter
    Granularity
    Flow Cytometry

    Granulocytes

    AfraTafreeh.com
    Side Scatter

    Monocytes

    Lymphocytes

    Front Scatter
    AfraTafreeh.com

    Antibody Staining
    • Specific antibodies to surface/intracellular proteins
    • Tagged with unique fluorochrome
    • Flow cytometer detects fluorochrome
    • Indicates presence of protein in cells
    Antibody Staining

    AfraTafreeh.com
    CD4

    CD8
    AfraTafreeh.com

    Flow Cytometry
    Clinical Uses

    • Fetal maternal hemorrhage


    • Fetal red cells cross placenta to maternal blood
    • Seen with placental failure/trauma
    • Presents as decreased fetal movement, abnormal fetal HR
    • Can cause stillbirth
    • Flow cytometry: monoclonal antibody to hemoglobin F
    • Detects fetal hemoglobin in red cells

    Øyvind Holmstad/Wikipedia
    Flow Cytometry
    Clinical Uses

    • Paroxysmal nocturnal hemoglobinuria


    • Fluorescently-labeled monoclonal antibodies
    • Bind glycosylphosphatidylinositol (GPI) anchored proteins
    • Decay Accelerating Factor (DAF/CD55)
    • MAC inhibitory proteinAfraTafreeh.com
    (CD59)
    • Reduced or absent on red blood cells in PNH

    Databese Center for Life Science (DBCLS)

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