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RESEARCH ARTICLE
Previous (/rd/RD14461) Contents Vol 27(6) (/rd/issue/7306)
Antioxidant supplementation during in vitro culture improves

mitochondrial function and development of embryos from aged
female mice
(/rd/PublishingPolicies)
Elena Silva A C , Alison F. Greene A , Kevin Strauss A , Jason R. Herrick A , William B. Schoolcraft B and Rebecca L. Krisher A
+ Author Affiliations BUY PDF $35.00
Reproduction, Fertility and Development 27(6) 975-983 https://doi.org/10.1071/RD14474
Submitted: 28 November 2014 Accepted: 20 January 2015 Published: 5 March 2015 Rent Article (via Deepdyve)
(https://www.deepdyve.com/doc-
view?
Abstract docId=10.1071/RD14474&fieldNam
Publishing)
Maternal aging results in reduced oocyte and blastocyst quality, thought to be due, in part, to mitochondrial Export Citation
dysfunction and accumulation of reactive oxygen species. To reduce oxidative stress, the antioxidants α-lipoic acid (/rd/ExportCitation/RD14474)
(ALA; 10 µM), α-tocopherol (250 µM), hypotaurine (1 mM) and N-acetylcysteine (NAC; 1 mM), and sirtuin (100 ng mL–1) Cited By (44)
were added to embryo culture medium (AntiOX) and compared with a control (CON) without antioxidants to assess (/rd/CrossrefCites/RD14474)
blastocyst development after in vitro maturation and fertilisation of oocytes from aged B6D2F1 female mice (13.5 Get Permission
months). Development to the blastocyst stage increased in the AntiOX compared with CON group (87.6% vs 72.7%, (https://www.copyright.com/openu
respectively; P < 0.01), in addition to higher mitochondrial membrane potential and ATP levels in the AntiOX group. contentIdType=doi&issn=1031-
Expression of genes associated with oxidative stress (PI3K, FOXO3A and GLRX2) was upregulated in the CON 3613&contentID=10.1071/RD14474
compared with AntiOX group. In addition to AntiOX, a medium containing only NAC and ALA (rAntiOX) was used to
culture embryos from young CF1 females (6–8 weeks). More blastocysts developed in the rAntiOX compared with View Dimensions
CON group (64.1% vs 43.3%, respectively; P < 0.01), although AntiOX (48.0% blastocysts) did not result in improved
development in young mice. Antioxidants improved mitochondrial activity, gene expression and development in
52
embryos of older female mice, whereas a reduced level of antioxidants during culture was beneficial to embryos
from young mice. (https://badge.dimensions.ai/detail
domain=https://www.publish.csiro.
Additional keywords: culture medium, oocyte, oxidative stress.
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Antioxidant supplementation during in vitro culture improves

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