CONTINUOUS AND DISCONTINUOUS

REPLICATION
 CONTINUOUS AND DISCONTINUOUS
REPLICATION

5’ 3’

5’

3’ 5’

3’ 5’ 3’
Direction of
Replication
DNA
polymerase acts in 5’ to 3’
direction of new strand 3’ 5’
CONTINUOUS AND DISCONTINUOUS
REPLICATION

New Strand = Leading Strand

Helicase 3’
Creating Fork
5’
5’
3’

Polymerase direction on
this strand, towards fork
CONTINUOUS AND DISCONTINUOUS
REPLICATION

Done here!
5’ go back to fork
3’ 5’
3’ 3’
5’ L ag g
ing s
trand
Once done with the fragment of 5’ to
Polymerase direction on this 3’ then I twill go to the fork again thus
strand, away from the fork. creating fragments of the strands.
CONTINUOUS AND DISCONTINUOUS
REPLICATION

OKAZAKI Fragments

Enzyme Ligase joins the fragments.

 EVIDENCE
FOR
SEMI-CONSERVATIVE REPLICATION
MODELS OF DNA REPLICATION
 MODELS OF DNA REPLICATION

Semiconservative
replication of DNA
requires ATP.

SEPARATES

Original DNA Templates New strand (Red)with

each of the old strand formed
 MODELS OF DNA REPLICATION

Original DNA Old New copy

MODELS OF DNA REPLICATION
 MODELS OF DNA REPLICATION

100% 100% 50%

Both old DNA Both new DNA One old and

Strands Strands one new
DNA Strands
MODELS OF DNA REPLICATION
 METHOD OF
LABELLING DNA STRANDS
 M E T H OD O F
L AB E L L I NG DN A S T R A N D S

7P 7P
7n 8n

DNA can be labelled because

of different weights.
14N 15N
 M E T H OD O F
L AB E L L I NG DN A S T R A N D S

E. Coli
Bacteria
15N Isotope

A G T C

15N
15N
 M E T H OD O F
L AB E L L I NG DN A S T R A N D S

E. Coli
Bacteria
14N Isotope
 EVIDENCE FOR
SEMI-CONSERVATIVE REPLICATION

15N 14N
 EVIDENCE FOR
SEMI-CONSERVATIVE REPLICATION

14N 15N Mixed

transferred

test colony
 EVIDENCE FOR
SEMI-CONSERVATIVE REPLICATION

14N

15N

Weight which
was in between
14N and 15N
 EVIDENCE FOR
SEMI-CONSERVATIVE REPLICATION

Suppose if this was The weight was

14N
the result case then somewhere between
the conservative 14N and 15N. It

model was proven proves the semi-

but the observation conservative
15N was different. observation replication model.