2/9/24, 11:11 AM Tissue Processing Technical Details form

Tissue Processing Technical Details form

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Email *

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Name of the center *

Your answer

This is a required question

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Center number *

Your answer

How many runs you had performed of Tissue processing module in EQAS ? *

Your answer

This is a required question

Does the laboratory have a policy of documenting time of surgery? *

Yes

No

Does the laboratory document time of receipt of specimen? *

Yes

No

Name of the fixative used and also mention the pH ? *

Your answer

Name of the company of Tissue Processor? *

Your answer

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Tissue Processor used? *

Fully Automated

Rotary

Manual

Other:

Technology used in processor? *

Microwave

Vaccum

Other:

Grade of reagent *

Your answer

Number of cassettes loaded in machine (per machine) everyday? *

Your answer

Frequency of changing reagents in the Tissue Processor? *

Your answer

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Temperature and timing of formalin in processor? *

Your answer

Are you using graded Ethyl alcohol or Isopropanol in Tissue Processing? (If *
other please specify)

Graded Isopropanol

Graded Alcohol

Other:

Specify temperature and timing of reagents(Isopropanol or Ethanol) used in *

processor ?

Your answer

Temperature and timing of xylene in processor? *

Your answer

Temperature and timing of Paraffin in processor? *

Your answer

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Tissue embedding? *

Manual

Automated

Other:

Embedding? *

Wet

Dry

Company of Paraffin wax? *

Your answer

Temperature of wax dispenser of embedding machine (Melting point) *

Your answer

Microtome used? *

Automated

Semi automated

Manual

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Company of Microtome? *

Your answer

Section thickness( μm )? *

Your answer

Type of microtome blade using? *

Your answer

Do you do manual or automated staining ? *

Your answer

Specify the name of the machine if you are doing automated staining? *

Your answer

Specify colour index of Hematoxylin ? *

Your answer

Specify colour index of Eosin ? *

Your answer

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2/9/24, 11:11 AM Tissue Processing Technical Details form

Name of the company of Hematoxylin? *

Your answer

Name of the company of Eosin? *

Your answer

If you are using Graded Alcohol, Ethyl Alcohol or Isopropanol in H&E staining *
Please specify with pH?

Your answer

Type of Hematoxylin used in staining (If other please specify ) *

Harris Hematoxylin

Mayers Hematoxylin

Gill's Hematoxylin

Other:

Type of staining done? *

Regressive

Progressive

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What is used for bluing reagent in staining? *

Your answer

What is used to differentiate H&E slide in staining? (If other please specify) *

1% Acid Alcohol

Other:

Time taken to differentiate H&E slide in staining? *

Your answer

Frequency of changing staining solutions? *

Your answer

Frequency of changing Hematoxylin & Eosin? *

Your answer

Do you want to participate in decal run? *

Yes

No

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Do you want to participate in special stain run? *

Yes

No

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