The City School

Academic Year 2020-21

Class 8
Subject: Science
Topic: Food & Digestion
Worksheet # 07-A

Student’s Name: ___________________ Date: ___________

Lesson Objective: to use chemical tests to identify proteins, carbohydrates and fats.

Kindly refer to the textbook (ILSS Book 3); pg-1 & 2 and workbook 3 pg-1-4 Activity 1.1,
1.2, 1.3 & 1.4 for solving the questions below:

Q1: A sample of food was ground up with a little water, and separate samples of the
resulting pulp were tested with Benedict solution, iodine and Biuret reagent. In each case,
the resulting mixture was blue.
What information does this give you about the food sample? Give a reason for your answer.
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Q2: Encircle the correct option:

i. Which of the following tests for starch?

a. Iodine
b. Benedict's
c. Biuret
d. Emulsion

ii. Which of the following tests for protein?

a. Iodine
b. Benedicts
c. Biuret
d. Emulsion

iii. Which of the following tests for glucose?

a. Iodine
b. Benedicts
c. Biuret
d. Emulsion
iv. When starch is present iodine turns...
a. purple
b. blue-black
c. orange
d. brick red

v. When protein is present Biuret turns...

a. purple
b. blue-black
c. orange
d. brick red

vi. When large amounts of glucose is present Benedicts turns...

a. purple
b. blue-black
c. brick-red
d. green

vii. A negative test for starch will cause Iodine to stay...

a. orange/red
b. blue
c. black
d. brick red

viii. A negative test for protein will cause Biuret to stay...

a. brick red
b. blue
c. black
d. orange

ix. A negative test for glucose will cause Benedicts to stay...

a. brick red
b. blue
c. black
d. orange

x. Which chemicals did you mix to test for proteins - biuret test?
a. Ethanol and water
b. Iodine and potassium permanganate
c. Benedicts Solution
d. Sodium Hydroxide and Copper Sulphate

xi. How do you test for lipids?

a. By using Ethanol and water
b. By using Benedicts solution
c. By using Iodine
d. By using Biuret test
xii. What is a positive result for the lipid emulsion test?
a. milky white colour
b. clear
c. yellow
d. green

xiii. In this experiment, you must put your test tubes into a hot water bath
a. Benedict's Test
b. Biurets Test
c. Iodine Test
d. Ethanol Emulsion Test

xiv. The building blocks of proteins are

a. carbohydrates
b. amino acids
c. sugars
d. fatty acids

xv. If you drop iodine solution on bread, it changes to Black. What does this show?
a. Bread contains protein
b. Bread contains sugar
c. Bread contains starch
d. Bread contains lipids?

xvi. This photo represents a

a. negative test for glucose

b. negative test for protein
c. positive test for starch
d. positive test for glucose
xvii. The test tubes in the photo represent a negative and positive result for

a. Benedict's with glucose

b. Biuret with protein
c. Iodine with starch
d. Ethanol with fat

xviii. The building blocks of lipids are

a. Starches
b. Fatty acids and Glycerol
c. Sugars
d. Amino Acids

xix. The building blocks of starch are

a. glycerol
b. amino acids
c. sugars
d. fatty acids

xx. Which biomolecule turns iodine solution black?

a. complex carbohydrate (polysaccharide)

b. protein
c. simple carbohydrate (monosaccharide)
d. lipid