Edition: 146

Chapter 2 Hydraulics/Mechanicals

2.1 Principle and Reagents .......................................................................................................................1

2.2 System Flow ........................................................................................................................................1

2.3 Sample Aspiration ...............................................................................................................................2

2.3.1 Whole Blood Sample Aspiration ........................................................................................2
2.3.2 Body Fluid Sample Aspiration............................................................................................3
2.3.3 PD (Pre-diluted) Sample Aspiration ...................................................................................3

2.4 Sample Aspiration Check ....................................................................................................................4

2.5 Blood Cannot Be Aspirated Error ........................................................................................................4

2.6 Insufficient Blood Volume (Short Sample) Error ..................................................................................5

2.7 Mixing Method .....................................................................................................................................6

2.7.1 Mixing Sequence ...............................................................................................................6

2.8 Whole Blood Analysis..........................................................................................................................8

2.8.1 RBC/PLT Analysis..............................................................................................................8
2.8.2 HGB Analysis.....................................................................................................................8
2.8.3 WDF Analysis ....................................................................................................................9
2.8.4 WNR Analysis ....................................................................................................................9
2.8.5 PLT-F Analysis .................................................................................................................10
2.8.6 WPC Analysis ..................................................................................................................10
2.8.7 RET Analysis ...................................................................................................................11

2.9 Body Fluid Analysis ...........................................................................................................................12

2.9.1 RBC Analysis (BF) ...........................................................................................................12
2.9.2 WBC Analysis (BF) ..........................................................................................................12

2.10 Pneumatic System...........................................................................................................................13

2.11 Hydraulic System.............................................................................................................................14

2.11.1 Use of Waste Chamber ..................................................................................................14
2.11.2 DV driven .......................................................................................................................14
2.11.3 Pinch Valve Driven .........................................................................................................15
2.11.4 Use of Solenoid Valve [AJ] .............................................................................................15

2.12 Optical System ................................................................................................................................18

2.12.1 FCM Detector Block.......................................................................................................18
2.12.2 FCM Block ASSY NO.15 ...............................................................................................18

2.13 Analysis Flow...................................................................................................................................19

2.13.1 RBC/PLT Analysis..........................................................................................................19
2.13.2 HGB Analysis.................................................................................................................21
2.13.3 FCM Analysis.................................................................................................................22

XN-1000/1500/2000 S/M April 2018

2.14 Hydraulic/Mechanical Parts .............................................................................................................25
2.14.1 Front Side Instrument ....................................................................................................25
2.14.2 ASP_ASSY NO.31 (XN-20) ...........................................................................................25
2.14.3 ASP_ASSY NO.31 (XN-10) ...........................................................................................26
2.14.4 REACTOR_ASSY NO.8 (XN-20) ...................................................................................26
2.14.5 REACTOR_ASSY NO.8(XN-10) ....................................................................................27
2.14.6 Left Side Instrument.......................................................................................................27
2.14.7 SENSOR_ASSY NO.10 (XN-20) ...................................................................................28
2.14.8 SENSOR_ASSY NO.10 (XN-10) ...................................................................................28
2.14.9 Right Side Instrument ....................................................................................................29
2.14.10 VALVE_ASSY NO.49 (XN-20) .....................................................................................29
2.14.11 VALVE_ASSY NO.49 (XN-10)......................................................................................30
2.14.12 VALVE_ASSY NO.148 (XN-20) ...................................................................................30
2.14.13 VALVE_ASSY NO.148 (XN-10) ...................................................................................31
2.14.14 VOLUMETRIC MEASURE ASSY NO.6 (XN-20) .........................................................31
2.14.15 VOLUMETRIC MEASURE ASSY NO.6 (XN-10) .........................................................32
2.14.16 Right Side Instrument (Other Assy) .............................................................................32
2.14.17 SENSOR_ASSY NO.26 (XN-20) .................................................................................33
2.14.18 SENSOR_ASSY NO.26 (XN-10) .................................................................................33
2.14.19 Back Side Instrument...................................................................................................34

XN-1000/1500/2000 S/M April 2018

Chapter 2 Hydraulics / Mechanicals

2.1 Principle and Reagents

Detecting Method Channel Reagent Parameter

WDF LYSERCELL WDF NEUT, LYMPH,

[NEW] FLUOROCELL WDF MONO, EO, IG

WNR LYSERCELL WNR WBC, NRBC,

[NEW] FLUOROCELL WNR BASO

CELLPACK DFL RET, RET-He,

FCM with Laser Diode RET/PLT-O
FLUOROCELL RET PLT-O
CELLPACK DCL
or
WPC LYSERCELL WPC
CELLPACK DST Flagging
[NEW] FLUOROCELL WPC
as Sheath Solution

PLT-F CELLPACK DFL

PLT-F, IPF
[NEW] FLUOROCELL PLT

Hydrodynamic Focusing RBC, HCT,

RBC/PLT
DC Method PLT-I

SLS-Hemoglobin Method HGB SULFOLYSER HGB

2.2 System Flow

[CR]
[CR] 2018034

XN-1000/1500/2000 S/M 2-1 April 2018

2.3 Sample Aspiration

2.3.1 Whole Blood Sample Aspiration

Whole Blood Aspiration (88µL)

Sample Volume
# Use
① Ver.00-14 or Lower Ver.00-15 or Higher

① Tail end discard portion 13µL 13µL

②
③ ② Sample aspiration check 2µL 2µL

④ ③ RET Sample 5µL 5µL

④ WPC Sample 17µL 17µL

⑤
⑤ PLT-F Sample 5µL 5µL
⑥ ⑥ WNR Sample 17µL 17µL

⑦ ⑦ WDF Sample 17µL 17µL

⑧ RBC/HGB Sample 4µL

1.4mm
4µL 1mm
⑧
⑨ ⑨ Sample Aspiration check 2µL 4µL

⑩ ⑩ Top end discard portion 6µL 4µL

313B037

XN-1000/1500/2000 S/M 2-2 April 2018

2.3.2 Body Fluid Sample Aspiration

2.3.3 PD (Pre-diluted) Sample Aspiration

[BB]

[BB] 2017027

XN-1000/1500/2000 S/M 2-3 April 2018

2.4 Sample Aspiration Check

① Tail end discard portion Aspiration sensor

② Sample aspiration check
③ RET sample

④ WPC sample

⑤ PLT-F sample
⑥ WNR sample

⑦ WDF sample
Only Ver.00-15 or higher
⑧ RBC/HGB sample RBC/HGB
⑨ Sample aspiration check sample chamber
⑩ Top end discard portion

Sample Volume for each usage

# 1 2 3 4 5 6 7 8 9 10

Ver.00-14 or lower 13uL 2 uL 5 uL 17 uL 5 uL 17 uL 17 uL 4 uL 2 uL 6 uL

Ver.00-15 or higher 13uL 2 uL 5 uL 17 uL 5 uL 17 uL 17 uL 4 uL 4 uL 4 uL

2.5 Blood Cannot Be Aspirated Error

①Tail end discard portion
②Sample aspiration check = Sample convert value 2

③Sample aspiration check = Sample convert value 1

④Top end discard portion

Sample Volume for each usage

# 1 2 3 4

Ver.00-14 or lower 13uL 2 uL 2 uL 6 uL

Ver.00-15 or higher 13uL 2 uL 4 uL 4 uL

Sample Not Aspiration Error

The difference between sample convert 2 value and blank convert value becomes below 3400.

Sample Convert 2 - Blank Convert ≤ 3400

313B037

XN-1000/1500/2000 S/M 2-4 April 2018

2.6 Insufficient Blood Volume (Short Sample) Error
① Tail end discard portion
② Sample aspiration check = Sample convert value 2

③ RBC/HGB Sample = Sample convert value 3

④ Sample aspiration check = Sample convert value 1
⑤ Top end discard portion

Sample Volume for each usage

# 1 2 3 4 5

Ver.00-14 or lower 13uL 2 uL 4 uL 2 uL 6 uL

Ver.00-15 or higher 13uL 2 uL 4 uL 4 uL 4 uL

Short Sample Error

Ver.00-14 or lower
The difference between Sample Convert value 1 and Blank Convert Value becomes below 1700.
Sample Convert Value 1 - Blank Convert Value ≤ 1700

The difference between Sample Convert value 1 and Sample Convert Value 2 is too big or too small.
(Sample Convert Value 1- Blank Convert Value)/(Sample Convert Value 2 - Blank Convert Value) x 100 ≤ 30 or ≥ 100

Ver.00-15 or higher
Using sample convert value 1 ~ 3 and blank convertion value, set variable A ~ F as below.
A = Sample Conversion Value 1 – Blank Conversion Value
B = Sample Conversion Value 2 – Blank Conversion Value
C = A/B x 100
D = Sample Conversion Value 3 - Blank Conersion Value
E = D/B x 100
F = A/D x 100
When matching with any of following conditions, this error will occur.
- A is 3400 or less
- C is 70 or less ; C is 200 or more
- D is 3400 or less
- E is 70 or less; E is 200 or more
- F is 200 or more
313B037 313B037-R

XN-1000/1500/2000 S/M 2-5 April 2018

2.7 Mixing Method
Mixing method is the same as XS.

2.7.1 Mixing Sequence

RBC/PLT

1. After draining rinsing reagent.

2. Add 1 mL CELLPACK by DP 1st dispense.
3. Add 4µL blood.
4. Right after blood dispense, add 1mL CELLPACK by DP 2nd dispense. Also pipette moves right
and left for mixing.

XN-1000/1500/2000 S/M 2-6 April 2018

FCM (e.g. WNR)

1. After draining rinsing reagent.

2. Add 0.5mL LYSERCELL WNR by DP 1st dispense.
3. Add 17µL blood.
4. Right after blood dispense, add 0.5mL LYSERCELL by DP 2nd dispense and add 20µL
FLUOROCELL WNR by dye DP one dispense at almost same time. Also, pipette moves right
and left for mixing.

XN-1000/1500/2000 S/M 2-7 April 2018

2.8 Whole Blood Analysis
2.8.1 RBC/PLT Analysis

[CR]

2.8.2 HGB Analysis

After RBC sample is taken by charging DP, add SULFOLYSER.

[CR]
[CR] 2018034

XN-1000/1500/2000 S/M 2-8 April 2018

2.8.3 WDF Analysis

2.8.4 WNR Analysis

XN-1000/1500/2000 S/M 2-9 April 2018

2.8.5 PLT-F Analysis

[CR]

2.8.6 WPC Analysis

[CR] 2018034

XN-1000/1500/2000 S/M 2-10 April 2018

2.8.7 RET Analysis

[CR]

[CR] 2018034

XN-1000/1500/2000 S/M 2-11 April 2018

2.9 Body Fluid Analysis
2.9.1 RBC Analysis (BF)

[CR]

2.9.2 WBC Analysis (BF)

[CR] 2018034

XN-1000/1500/2000 S/M 2-12 April 2018

2.10 Pneumatic System

Use of Pressure and Vacuum

Type Name Functions

Driving Master Valve

0.25MPa Driving air cylinder
Driving diaphragm pump (RBC and FCM sheath only)

Pressure 0.16MPa Sheath flow forming for FCM

Sheath flow forming for RBC

0.07MPa Driving diaphragm pump
Draining WC1

Source Vacuum NOT used

Vacuum
Aspiration of waste to WC1
-0.04MPa
Driving diaphragm pump

XN-1000/1500/2000 S/M 2-13 April 2018

2.11 Hydraulic System
2.11.1 Use of Waste Chamber
Chamber Standby Status Functions

Rinsed dilution and waste are collected

WC1 Vacuumed
Waste is drained to external waste tank

FCM and RBC wastes are corrected

WC2 Opened
Waste is drained to WC1

2.11.2 DV driven
6 DSV Drive Multiple DP

DSV 25 & 26 DP_AIR 1

LYSERCELL-WNR
LYSERCELL WPC

DSV 31 & 32 DP_AIR 2

LYSERCELL-WDF
CELLPACK DFL

DSV 3 & 4 ---

All FLUOROCELL

XN-1000/1500/2000 S/M 2-14 April 2018

2.11.3 Pinch Valve Driven

Status PV81 PV71

Stand-by Close Open

During WC1 draining Open Close

During Aspiration Open Open

2.11.4 Use of Solenoid Valve [AJ]

SV No Function Type SV No Function Type

WPC Stain Discharging DP

1 45 valve assy no.24 (reserved)
(Chamber side) (not on XN-10)

WPC Stain Discharging DP (Bottle

2 46 valve assy no.24 (reserved)
side) (not on XN-10)

3 Stain DP Pressure DS 47 Not Used (see wiring diagram)

4 Stain DP Vacuum DS 48 Not Used (see wiring diagram)

WDF Stain Discharging DP WB Aspiration Line

5 DS 49
(Chamber side) (Aspirating with ON)

WDF Stain Discharging DP

6 DS 50 Cell Clean Aspiration DS
(Bottle side)

7 Hgb Cell Rinse DS 51 WB Aspiration Pump Rinse DS

XN-1000/1500/2000 S/M 2-15 April 2018

 8 RBC/Hgb Chamber Rinse DS 52 RBC Sheath Syringe Rinse DS

WNR Stain Discharging DP

9 DS 53 Not Used DS
(Chamber side)

WNR Stain Discharging DP

10 DS 54 Not Used
(Bottle side)

11 WPC Reaction Chamber Cleaning 55 FCM Sheath Replenish DP1

12 WDF Reaction Chamber Cleaning DS 56 Sample Tube Rotating (Release)

RET Stain Discharging DP

13 DS 57 Sample Tube Rotating (Rotation)
(Chamber side)

RET Stain Discharging DP

14 DS 58 RBC Charging Line (IN)
(Bottle side)

15 WNR Reaction Chamber Cleaning DS 59 Hgb Charging (OUT)

FCM Charging Line Cleaning by

16 RET Reaction Chamber Cleaning DS 60
WNR Lyse Reagent

PLT Stain Discharging DP

17 DS 61 WPC Charging Line (not on XN-10)
(Chamber side)

PLT Stain Discharging DP

18 DS 62 WDF Charging Line
(Bottle side)

19 RBC Front Flash (Exit side) DS 63 WNR Charging Line

20 RBC Sheath Formation DS 64 RET Charging Line

WPC Reaction Chamber Drain

21 Not Used 65
(not on XN-10)

22 RBC Front Flash (Enter side) DS 66 WDF Reaction Chamber

23 RBC Back Flash DS 67 WNR Reactin Chamber Drain

24 Isolation Chamber drain DS 68 RET Reaction Chamber Drain

FCM Lyse Reagent (WNR, WPC)

25 DS 69 RBC/Hgb Chamber Drain
DP Pressure

FCM Lyse Reagent (WNR, WPC)

26 DS 70 RBC Charging Line (OUT)
DP Vacuum

WPC Lyse Reagent Discharging DP Rinse Cup Drain PV (rinse cup

27 71
(Chamber side) (not on XN-10) side)(Open with OFF)

WPC Lyse Reagent Discharging DP

28 77 FCM Sheath Pressure
(Bottle side) (not on XN-10)

WNR Lyse Reagent Discharging DP

29 DS 78 FCM Sheath Replenish DP2
(Chamber side)

WNR Lyse Reagent Discharging DP

30 DS 79 FCM Charging DP
(Bottle side)

FCM Lyse Reagent (WDF, RET) DP Hand Catch and Release

31 DS 80
Pressure Mechanism

XN-1000/1500/2000 S/M 2-16 April 2018

 FCM Lyse Reagent (WDF, RET) DP Rinse Cup (WC1 side) Drain PV
32 DS 81
Vacuum (open with ON)

WDF Lyse Reagent Discharging DP FCM Sheath Syringe Enhanced

33 DS 82
(Chamber side) Rinse

WDF Lyse Reagent Discharging DP

34 DS 83 High-speed Sheath Formation
(Bottle side)

RET Diluent Discharging DP

35 DS 84 FCM Sheath Syringe Cleaning
(Chamber side)

RET Diluent Discharging DP

36 DS 85 Low-speed Sheath Formation
(Bottle side)

37 Collecting FCM Sheath (Exit) 86 Air Filter Drain

38 DIL (Cellpack DCL) Replenish Line DS 87 WC1 Drain (all)

DIL (Cellpack DCL) Chamber

39 DS 89 Hgb Lyse Reagent Discharging DP
Pressure

DIL (Cellpack DCL) Chamber RBC/Hgb Diluent Solution

40 DS 90
Vacuum Discharging DP

WC2 (Sheath Waste) Drain PV

41 RBC Sheath Chamber Pressure DS 91
(Open with ON)

42 Rinse Cup Fill DS 92 RBC Sheath Chamber Replenish DP

Dil (EPK) Air Bubble Removal &

43 DS 93 RBC/Hgb Charging DP
Chamber fill

RBC (EPK) Air Bubble Removal &

44
Chamber fill

STM1 Aspiration Unit Up & Down Motor STM6 Sample Tube Clamp Motor

STM2 RBC Sheath Motor STM7 Sample Set B/F Motor

STM3 FCM Sheath Motor STM8 Hand U/D Motor

STM4 WB Aspiration Motor STM9 Sample Tube Mixing Motor

STM5 Aspiration Unit Right & Left Motor STM10 Sample Tube Rotating Motor

[AJ] 2016124

XN-1000/1500/2000 S/M 2-17 April 2018

2.12 Optical System
2.12.1 FCM Detector Block

2.12.2 FCM Block ASSY NO.15

Laser Specification

Wave Length 639nm

3.4mW 6mW 10mW

Laser Power
WNR, WPC WDF RET, PLT-F

XN-1000/1500/2000 S/M 2-18 April 2018

2.13 Analysis Flow
2.13.1 RBC/PLT Analysis

RBC/PLT Analysis 1

RBC/PLT Analysis 2

XN-1000/1500/2000 S/M 2-19 April 2018

RBC/PLT Analysis 3

RBC/PLT Analysis 4

XN-1000/1500/2000 S/M 2-20 April 2018

RBC/PLT Analysis 5

2.13.2 HGB Analysis

After RBC sample is taken by charging DP, add SULFOLYSER.

XN-1000/1500/2000 S/M 2-21 April 2018

2.13.3 FCM Analysis

FCM Analysis 1

FCM Analysis 2

XN-1000/1500/2000 S/M 2-22 April 2018

FCM Analysis 3

FCM Analysis 4

XN-1000/1500/2000 S/M 2-23 April 2018

FCM Analysis 5

FCM Analysis 6

XN-1000/1500/2000 S/M 2-24 April 2018

2.14 Hydraulic/Mechanical Parts
2.14.1 Front Side Instrument

2.14.2 ASP_ASSY NO.31 (XN-20)

XN-1000/1500/2000 S/M 2-25 April 2018

2.14.3 ASP_ASSY NO.31 (XN-10)

2.14.4 REACTOR_ASSY NO.8 (XN-20)

XN-1000/1500/2000 S/M 2-26 April 2018

2.14.5 REACTOR_ASSY NO.8(XN-10)

2.14.6 Left Side Instrument

XN-1000/1500/2000 S/M 2-27 April 2018

2.14.7 SENSOR_ASSY NO.10 (XN-20)

2.14.8 SENSOR_ASSY NO.10 (XN-10)

XN-1000/1500/2000 S/M 2-28 April 2018

2.14.9 Right Side Instrument

2015089

2.14.10 VALVE_ASSY NO.49 (XN-20)

XN-1000/1500/2000 S/M 2-29 April 2018

2.14.11 VALVE_ASSY NO.49 (XN-10)

2.14.12 VALVE_ASSY NO.148 (XN-20)

XN-1000/1500/2000 S/M 2-30 April 2018

2.14.13 VALVE_ASSY NO.148 (XN-10)

2.14.14 VOLUMETRIC MEASURE ASSY NO.6 (XN-20)

XN-1000/1500/2000 S/M 2-31 April 2018

2.14.15 VOLUMETRIC MEASURE ASSY NO.6 (XN-10)

2.14.16 Right Side Instrument (Other Assy)

XN-1000/1500/2000 S/M 2-32 April 2018

2.14.17 SENSOR_ASSY NO.26 (XN-20)

2.14.18 SENSOR_ASSY NO.26 (XN-10)

XN-1000/1500/2000 S/M 2-33 April 2018

2.14.19 Back Side Instrument

XN-1000/1500/2000 S/M 2-34 April 2018