Chapter 2 Hydraulics / Mechanicals

2.1 Unit Layout................................................................................................................................. 1

2.1.1 Main Unit Front View ............................................................................................................1
2.1.2 Main Unit Left View ...............................................................................................................3
2.1.3 Main Unit Right View ............................................................................................................5
2.1.4 Main Unit Rear View .............................................................................................................7
2.1.5 Main Unit Top View ...............................................................................................................8

2.2 Principle and Reagents............................................................................................................ 10

2.3 System Flow ............................................................................................................................ 10

2.4 Sample Aspiration.................................................................................................................... 11

2.4.1 Whole Blood Sample Aspiration .........................................................................................11
2.4.2 Body Fluid Sample Aspiration .............................................................................................12
2.4.3 PD (Pre-diluted) Sample Aspiration ....................................................................................12

2.5 Sample Aspiration Check......................................................................................................... 13

2.6 Blood Cannot Be Aspirated Error............................................................................................. 13

2.7 Insufficient Blood Volume (Short Sample) Error ...................................................................... 14

2.8 Mixing Method.......................................................................................................................... 15

2.8.1 Mixing Sequence ................................................................................................................16

2.9 Whole Blood Analysis .............................................................................................................. 18

2.9.1 RBC/PLT Analysis ..............................................................................................................18
2.9.2 HGB Analysis ......................................................................................................................18
2.9.3 WDF Analysis .....................................................................................................................19
2.9.4 RET Analysis ......................................................................................................................19

2.10 Body Fluid Analysis.................................................................................................................. 20

2.10.1 RBC Analysis (BF) ..............................................................................................................20
2.10.2 WBC Analysis (BF) .............................................................................................................20

2.11 Pneumatic System ................................................................................................................... 21

2.12 Hydraulic System ..................................................................................................................... 21

2.12.1 Use of Waste Chamber ......................................................................................................21

2.13 Optical System......................................................................................................................... 22

2.13.1 FCM Detector Block ............................................................................................................22
2.13.2 FCM BLOCK ASSY NO.23 .................................................................................................23

2.14 Analysis Flow .......................................................................................................................... 26

2.14.1 RBC/PLT Analysis Flow ......................................................................................................26
2.14.2 HGB Analysis Flow .............................................................................................................28
2.14.3 FCM Analysis Flow .............................................................................................................29

XN-L S/M May 2015

Chapter 2 Hydraulics / Mechanicals
2.1 Unit Layout

2.1.1 Main Unit Front View

[XN-350/330]

XN-L S/M 2-1 May 2015

[XN-450/430]

[XN-550/530]

XN-L S/M 2-2 May 2015

2.1.2 Main Unit Left View
[XN-350/330]

XN-L S/M 2-3 May 2015

[XN-450/430]

[XN-550/530]

XN-L S/M 2-4 May 2015

2.1.3 Main Unit Right View
[XN-350/330]

[XN-450/430]

XN-L S/M 2-5 May 2015

[XN-550/530]

XN-L S/M 2-6 May 2015

2.1.4 Main Unit Rear View
[XN-350/330]

[XN-450/430/550/530]

XN-L S/M 2-7 May 2015

2.1.5 Main Unit Top View
[XN-350/330/450/430]

XN-L S/M 2-8 May 2015

[XN-550/530]

XN-L S/M 2-9 May 2015

2.2 Principle and Reagents

Detecting Channel Reagent Parameter

Method Used as
Labelling Used as
Lysing reagent sheath
reagent diluent
fluid
NEUT,
LYMPH,
WDF LYSERCELL FLUOROCELL
- MONO, EO,
[NEW] WDF WDF
FCM with IG, WBC,
Laser Diode CELLPACK BASO
DCL or
RET/PLT- FLUOROCELL CELLPACK CELLPACK RET, RET-
-
O RET DFL DST He, PLT-O

Sheath
RBC, HCT,
Flow DC RBC/PLT - - CELLPACK PLT-I
Method DCL or
SLS- CELLPACK
Hemoglobin HGB SULFOLYSER - DST - HGB
Method

2.3 System Flow

Piercer RBC/HGB chamber

RBC/HGB sample
CELLPACK 4.0μL RBC detector
(1:503)
2mL HGB flow cell(1:755)
SULFOLYSER
500μL

Reaction chamber

WDF sample
Lysercell-WDF 11.0μL Optical detector
(1:94)
1mL
Fluorocell-WDF
20μL

Fluorocell-RET CELLPACK
20μL
CELLPAK-DFL 4.0μL RET sample
(1:257)
1mL

XN-L S/M 2-10 May 2015

2.4 Sample Aspiration

2.4.1 Whole Blood Sample Aspiration

1.4mm 1mm

XN-450/550
2015057

XN-L S/M 2-11 May 2015

2.4.2 Body Fluid Sample Aspiration

(* Errors are not determined during sample aspiration check)

2.4.3 PD (Pre-diluted) Sample Aspiration

(* Errors are not determined during sample aspiration check)

XN-L S/M 2-12 May 2015

2.5 Sample Aspiration Check

2.6 Blood Cannot Be Aspirated Error

Blood Cannot Be Aspirated Error

The difference between sample convert value 2 and blank convert value becomes 3400 or less.
Sample Conversion Value 2 - Blank Conversion is less than or equal to 3400

XN-L S/M 2-13 May 2015

2.7 Insufficient Blood Volume (Short Sample) Error

During whole blood mode measurement (except for blank check), the following conversion values and
blank conversion value are compared by blood aspiration sensor.

Sample Conversion Value 1 : Value converted (detected) from the turbidity of piercer top end dis-
card portion which is diluted with CELLPACK DCL before RBC sam-
ple is aspirated.
Sample Conversion Value 2 ：Value converted (detected) from the turbidity of whole blood pump
discard portion which is diluted with CELLPACK DCL before WBC
sample is dispensed.
Sample Conversion Value 3 ：Value converted (detected) from the turbidity of RBC sample diluted
with CELLPACK DCL

Using sample conversion value 1 ~ 3 and blank conversion value, set variables A ~ F as below.
A = Sample Conversion Value 1 - Blank Conversion Value
B = Sample Conversion Value 2 - Blank Conversion Value
C = A / B * 100
D = Sample Conversion Value 3 - Blank Conversion Value
E = D / B * 100
F = A / D * 100

When matching with any of following conditions, this error will occur.
• A is 1700 or less
• C is 35 or less, or C is 100 or more
• D is 3400 or less
• E is 70 or less, or E is 200 or more
• F is 100 or more

XN-L S/M 2-14 May 2015

2.8 Mixing Method
Mixing method is the same as the one for XS and XN.(Swirl mixing)

XN-L S/M 2-15 May 2015

2.8.1 Mixing Sequence
RBC/PLT

(1) Rinsing reagent is drained.

(2) DP1 dispenses 1mL CELLPACK.
(3) 4μL blood sample is added.
(4) While pipette moves right and left, blood sample is added. Also, DP dispenses 1mL CELLPACK for
mixing.

XN-L S/M 2-16 May 2015

FCM (e.g. WDF)

(1) Rinsing reagent is drained.

(2) DP dispenses 0.5mL LYSERCELL WDF.
(3) 11μL blood sample is added.
(4) While pipette moves right and left, blood sample is added. Also, DP dispense 0.5mL LYSERCELL
and 20μL FLUOROCELL almost at the same time for mixing.

XN-L S/M 2-17 May 2015

2.9 Whole Blood Analysis

2.9.1 RBC/PLT Analysis

2.9.2 HGB Analysis

After RBC sample is taken by charging DP, SULFOLYSER is added.

XN-L S/M 2-18 May 2015

2.9.3 WDF Analysis

2.9.4 RET Analysis

XN-L S/M 2-19 May 2015

2.10 Body Fluid Analysis

2.10.1 RBC Analysis (BF)

2.10.2 WBC Analysis (BF)

XN-L S/M 2-20 May 2015

2.11 Pneumatic System
The pneumatic system needed for XN-L operation consists of 2 pneumatic outputs, +0.06Mpa pressure,
and -0.03Mpa vacuum. These pneumatic outputs are generated by built-in air pump unit and regulated to
+0.06 Mpa and -0.03 Mpa by corresponding relief valves respectively. These relief valves have no
adjustment points.

2.12 Hydraulic System

2.12.1 Use of Waste Chamber

Chamber- Standby Status Fimctopms
1. Rinsed dilution and waste are collected
WC1 Vacuumed
2. Waste is drained to external waste tank
1. FCM and RBC wastes are corrected
WC2 Opened
2. Waste is drained to WC1

XN-L S/M 2-21 May 2015

2.13 Optical System

2.13.1 FCM Detector Block

No. Generic Name Part Name

1 Laser diode LASER DIODE HL6367DG
2 Optical plate GLASS PLATE NO.102
3 Colimeter lens OBJECTIVE LENS 4B57-20
4 Cylindrical lens LENS NO.169
5 Condenser lens LENS NO.170
6 Glass plate GLASS PLATE NO.117
7 Objective lens LENS NO.166
8 Objective lens LENS NO.165
9 Beam stopper APERTURE NO.153
10 Interference filter OPTICAL FILTER NO.136
11 Interference filter OPTICAL FILTER NO.136
12 Mirror MIRROR NO.131
13 Flowcell FLOWCELL NO.136
14 Photo diode (Forward scattered light) PHOTO DIODE S1223
Photo diode (Side fluorescence light, side
15 PHOTO DIODE S1223-01
scattered light)

XN-L S/M 2-22 May 2015

2.13.2 FCM BLOCK ASSY NO.23

Laser Specifications

Wave Length 633nm

Laser Power 45mW 45mW
WDF RET

XN-L S/M 2-23 May 2015

[Flowcell]

[Light Emitting Unit]

XN-L S/M 2-24 May 2015

[Forward Scattered Light Condensing Unit and Side Scattered Light Receiving Unit]

[Forward Scattered Light Receiving Unit]

XN-L S/M 2-25 May 2015

2.14 Analysis Flow

2.14.1 RBC/PLT Analysis Flow

RBC/PLT Analysis Flow 1

RBC-PLT Analysis Flow 2

XN-L S/M 2-26 May 2015

RBC/PLT Analysis Flow 3

RBC/PLT Analysis Flow 4

XN-L S/M 2-27 May 2015

RBC/PLT Analysis Flow 5

2.14.2 HGB Analysis Flow

After RBC sample is taken by charging DP, SULFOLYSER is added.

XN-L S/M 2-28 May 2015

2.14.3 FCM Analysis Flow
FCM Analysis Flow 1

FCM Analysis Flow 2

XN-L S/M 2-29 May 2015