BF-6800 Automatic hematology analyzer

Contents

Working Principle
Composition
Installation
Adjustment
Maintenance
Upgrade
Troubleshooting
Exam
 Principle

1. Working principle
WBC Testing -

Laser Flow Cell Technique&Impedance Method

Hemoglobin Concentration Testing-Colorimetry

RBC / PLT Testing- Impedance Method

 Principle

WBC Test

4 DIFF channel BASO channel

Semiconductor laser special hemolysin

flow cytometry+Staining
Technique impedance

LYM MON EOS NEUT+ BASO WBC

BASO
% % % %
%
 Principle

Diff Channel- Laser Flow Cell Technique

High angle
scattering
After the effect of a certain
amount of reagent, the WB Low angle
C sample is pipet into flow scattering
unit which is full of diluent
.
Under the package of she
ath liquid which is formed
by diluent, the single cell
pass through the center of
flow unit .
 Principle

Dual angle scattered light

High-angle forward scattered light
8~20° （ Cell inner complex structure ）

Low-angle forward scattered light

1~6° （ Cell size ）

Under the effect of laser beam, the scattered light chara

cter is related to cell size, refractive index of cell membr
ane and cell internal structure ,shape
 Principle

Testing
Optical system

Semi-conductor Laser:
Focusing
 Long life Testing
 Stability
Collimator
 Low consumption
Focusing light split
Collimator ：
Sheath-flow
 Precise angle control cell
Light source
 Eliminating stray light
 Accurate result
 Principle

Photodiode receive the scattered light

signals and translate them into electrical
pulses, according to the electrical pulses,
two-dimension map(scattergram) of cell
size and cell internal information can be
obtained.
The X-axis reflects the cell's internal
structure. The Y-axis reflects the cell size.
 Principle

Volume GR+BA scattergram

MO EO

GH LY
Cell internal structure
 Principle

Baso Channel- Impedance Method

 Principle

Electrical Impedance Method is based on

the measurement of changes in electrical
resistance produced by a particle passing
through an aperture.
As blood cells are poor conductive, when
they pass through the aperture, they
cause an increased impedance, the
impedance directly proportioned to the cell
size.
 Principle

SLS-I hemolysin dissolve RBC and break

WBC cell wall (except baso). Basophils keep
original form .All the other kinds of white
blood cells is shrank.According to the method
of impedance , Baso number and total
number of WBCs can be accurately counted.
。
Principle
 Principle

Hemoglobin Channel-Colorimetry
 The diluted sample is mixed with lyse reagent, RBC dissolve, releas
ing hemoglobin.
 Hemoglobin combined with lyse reagent to form hemoglobin complex.
 The hemoglobin complex is irradiated by monochromatic light with a w
avelength of 546nm .
 Phototube is used to receive transmission light, and convert the light sig
nal into voltage signal.
 By comparing with the voltage produced by background transmission li
ght intensity before sample adding (only diluent exists), the hemoglobin
concentration can be obtained(HGB), unit is g ／ L.

HGB=Constant × Ln background transmission light intensity

sample transmission light intensity
 Principle

4DIFF Channel ：
Reagent ：
BF-FDO Reagent ： stain, firming agent
BF-FDT Reagent: dissolve RBCs and specialize different types of WB
Cs ；
Diluent ： rinsing ， offering sheath

BASO Channel ：
Reagent:
SLS-I Reagent : dissolve RBCs, platelets,combined with hemoglobin a
nd remove cell membrane of WBCs exclude basophil; keep Baso main
tain the original form.
Diluent ： rinsing
 Principle

RBC / PLT Channel- Impedance Method

Electrical Impedance Method is based on
the measurement of changes in electrical
resistance produced by a particle passing
through an aperture.
As blood cells are poor conductive, when
they pass through the aperture, they
cause an increased impedance, the
impedance directly proportioned to the cell
size.
 Principle

Working principle
 Principle

RBC pulse

RBC low
limited

PLT
pulse
PLT low
limited

noise
 Principle

The number of pulses is equivalent to the cell number

through the aperture.

The pulse amplitude is proportional to the cell size.

All electrical pulse is classified according to different

channel voltage value.

Reagent ：
Diluent ： diluting, rinsing, offering conductive
environment, cells equal volume processing
 Principle

Product Name Spec. When When power When runs When runs
switch off (capillary (whole
on blood,CBC+DIFF blood,CBC+DIFF
Mode) Mode)

BF-Diluent 20L 120ML 150ML 60ML 60.18ML

BF-6800 Lyse(SL 500ML 2ML 2ML 0.5ML 0.5ML

S-Ⅰ)

BF-FDT Lyse 500ML 2ML 2ML 0.2ML 0.2ML

BF-FDO Lyse 5L 4ML 2ML 2ML 2ML

BF Detergent 10ML 0ML 0.3ML 0ML 0ML

 Composition

2.Composition
1
Front cover
2 Counting key
3 Mode key
4 Indicator
5 STAT rack
6 Tube rack
7 Auto loader
Composition

8 Fan

9 BF-SLS-I Inlet

10 BF-FDO Inlet

11 BF-FDT Inlet

12 BF-PK Diluent

13 Waste outlet
Composition

14 Optical Assembly
15 Sampler Assembly
16 Chamber
17 Pressure regulator
18 Sample syringe
19 Sheath liquid syringe
20 Valve Assembly
Four Syringes Assem
21
bly
Composition

22 Right door

23 Net port

24 Connector

25 Power filter
Composition
 Composition

The electrical part is consist of 8 parts:

Mainboard
Driver board
Data acquisition board (AD board )
•Temperature and pressure control board
Photoelectric receiver board
Power indicator board
Laser driver board
Power supply
 Composition

Main Board
Function :
Coordinate with other system module
Communicate with computer through net port
Provide RJ45 and RS232 port
Provide SPI for data acquisition
 Composition

Driver board ：
Function ：
Serial communication ( main board , AD board and
temp control board )
Drive the step motor （ sample motor 、 diluent motor 、 Lyse mot
or 、 test motor 、 X axis motor 、 Y axis motor ）
Drive the valve and pump （ 27 valves 、 1 positive pressure pump
、 2 vacuum pumps ）
Indicator control （ power indicator, status indicator, alarm indicator
）
Reagent supply control
Peripheral sensor control (8 float sensors,5 photo sensors,
5 switchs )
 Composition

Data acquisition (AD board )

Function:
WBC DIFF and BASO signal processing
RBC and PLT signal processing
HGB signal processing
Cell information storage
Analog channel control, magnification times setting
SPI communication port
Voltage monitoring
Aperture burn, sewage disposal
 Composition

Temperature and pressure control board

Function :
Temperature and pressure control
Temperature sensing: Diff premixed bath temperature
Laser unit temperature
Room temperature
Temperature control : Diff premixed bath temperature
Laser unit temperature
Pressure sensing : positive pressure
 Composition

Photoelectric receiver board

High angle and low angle receive board.
Function :
Photoelectrical Conversion and Pre-amplification
for high and low angle light signal .
 Composition

Laser driver board

Function:
Driving the semi-conductor laser unit
Keep output 4.5mW stable and adjustable power .
 Composition

Power indicator board

Function :
Indicate different status of the analyzer
power on ,error or running
 Composition

Switch Power supply

Function :
Provide 24VDC ,5VDC,±12VDC
24VDC : Motor ,valves , pumps and heaters
5VDC : each electrical board
±12VDC : Photoelectric receiver board
 Pipeline

The diluent ratio of diff channel

Whole blood 11 ul

FDO 1 ml

FDT 0.2ml

The ratio of diluent:1:110

 Pipeline

The diluent ratio of WBC and RBC

Whole blood 6ul

Diluent 3ml

the ratio of Aspirate 50ul diluent

diluent:1:500 sample to RBC/PLT bath

SlS-I :0.5ml Diluent:3ml

The ratio of
The ratio of diluent:1:30000 for
diluent:1:583 for WBC/
RBC and PLT
BASO and HGB
 Pipeline

Sample20ul

Dispense 11ul sample Dispense 6ul Sample in

in Diff bath WBC/HGB bath

Dispense 3ml diluent in

Dispense 1ml FDO WBC/HGB bath

Dispense 0.2mlFDT
Sample probe lift up an
d discharge residual 3ul
sample into swab
Aspirate 1 ml
mixed sample
Sample probe aspirate 5 Sample probe dispense
0ul diluent sample from 50ul diluent sample in R
Test in flowcell WBC/HGB bath BC/PLT bath

Dispense 0.5ml sls-I in

WBC/HGB bath
Dispense 3ml
Rinse flowcell Diluent
Mixing Mixing

Count WBC,test HGB Count RBC/PLT

Pipeline
 Pipeline

1.Sample probe dispense

Diff channel 11ul sample to DIFF pr
e-mixed bath .

2. FDT syringe inject 0.2ml

FDT liquid to Diff bath via
sv23 .
FDO syringe inject 1ml FDO t
o Diff bath via sv22 .
Sample probe mixing and air
bubble mixing
3.Test syringe aspirate the
sample to the pipeline from
sv24 to sv27.
4.Test syringe inject the s
ample to flow cell for Diff t
est
 Pipeline
1.Sample probe move to
WBC/BASO,HGB channel WBC bath and dispense
6ul sample

2.Diluent syringe inject 3m

l diluent in WBC/HGB bat
h

3.Sample probe lift up an

d discharge residual 3ul s
ample into swab

4.Sample probe aspirate 5

0ul diluent sample from
WBC/HGB bath

5.SLS syringe inject 0.5ml

sls-I to WBC/HGB bath

6.Air bubble mixing .Test

syringe aspirate the mixed
sample via sv10 and sv27
. the sample will not reach
the RBC bath.
 Pipeline

RBC/PLT channel 1.Sample probe move to R

BC&PLT counting bath and
dispense 50ul diluent samp
le
2.Diluent syringe inject 3ml
diluent to RBC&PLT counti
ng bath
3.Air bubble mixing .Test
syringe aspirate the mixed
sample via sv10 and
sv27 .
4.WBC and RBC are tested
at the same time .
 BF6500&BF6800 automatic
hematology analyzer

3.Installation
1.Intallation requirement
Space Requirement
Ensure enough space for instrument maintenance.
The space between the wall and the right & left side of the
instrument ≥50cm
The space between the wall and the back side of the instrum
ent≥50cm
The space between the front of the analyzer and other equip
ment ≥ 100cm;
Ensure enough space under and above the instrument for dil
uent, reagent and waste solution collecting devices.
 BF6500&BF6800 automatic
hematology analyzer

Power Requirement
Power : 100~240VAC 50/60Hz

Analyzer Rated Power :175VA

Fuse : 250V 4A ；

Large load equipment like air conditioner, refrigerator, ove

n etc. should not share the same power supply.

The voltage between the N~G should be less than 2V

 BF6500&BF6800 automatic
hematology analyzer

Environmental Requirement
Working Temperature ： 15℃-30℃ ；
Relative Humidity ：≤ 75 ％ ;
Atmospheric Pressure ： 75kPa-106kPa ；
The instrument should be protected from dust, mechanical vibration,
significant noise and power interference.
It is recommended that the electromagnetic environment assessment of the
laboratory should be conducted prior to test.
Do not use this instrument in close proximity to sources of strong electroma
gnetic radiation, as these may interfere with the proper working.
It should be placed far from the constant ON-OFF electrical devices like bru
sh-type motor, fluorescent lamp.
It should be placed far from heat and wind source, sunlight, brush-type mot
or, flickering fluorescent light and electrical contact equipment.
A well-ventilated place is a must.
 BF6500&BF6800 automatic
hematology analyzer

2. Unpacking
Check the packaging of the instrument. Contact Dirui or
local distributor for any physical damage.

Place the package in the direction of the arrow upward.

Open the accessory box, mainframe box, check the items

according to the packing list. Contact Dirui or local distributor
for any shortage.
Installation
 BF6500&BF6800 automatic
hematology analyzer

3.Intallation step
1. Connecting
a. Lyse, Diluent, Waste Solution Connecting
b. Liquid Level Sensor Connecting
c.Computer Connecting
d.Power Wire Connecting
e.Barcode Reader Connecting
f.Printer Connecting
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

Warning:
Safty screws should be removed before power o
n, otherwise analyzer may be damaged.
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

4.setup
Login the software and find the instrument IP as picture .
Set the computer IP as 172.16.100.XXX to get the communication .
 BF6500&BF6800 automatic
hematology analyzer

Scan the barcode for each reagent

 BF6500&BF6800 automatic
hematology analyzer

Replace all the reagent after scan the barcode

 BF6500&BF6800 automatic
hematology analyzer

Run blank test to meet the requirement as follow

 BF6500&BF6800 automatic
hematology analyzer

4.Adjustment
1. Sample probe

The life of puncture probe is around 30000 times

 BF6500&BF6800 automatic
hematology analyzer

The nozzle of the probe should face to the probe base board and at
the same level with outlet of swab
If necessary ,the vertical position sensor of probe could be adjust
also .
BF6500&BF6800 automatic
hematology analyzer

vertical position
sensor of probe
 BF6500&BF6800 automatic
hematology analyzer

2.Probe unit adjustment

The probe unit is fixed on the

base board with 6 screws as
picture show
 BF6500&BF6800 automatic
hematology analyzer

Enter the “service”—“Advanced” —“Optocoupler position

detect”
Run the “close test’’ to
check the probe position on
Stat position No.3
If the probe does not face
the center of No.3
position ,adjust the screws
which fix the probe unit on
the base board to fit it .
 BF6500&BF6800 automatic
hematology analyzer

3.Sample loader adjustment

After install or replace the sample loader ,it needs adjustment
as follow :
Enter the “service”—“Advanced” —“Optocoupler position
detect”
Run the “close test’’ Then the probe should move to the top
of No.3 STAT .
Then use paper or something to cover the second sensor of
probe unit .
 BF6500&BF6800 automatic
hematology analyzer

Then Enter the “service”—“Advanced” —“Mechanical detect

”
Input 6000 after the “Y-axis’’ and run it .
Then the probe should descend to the center of No.3
STAT .
If not ,adjust the sample loader to fit it .
 BF6500&BF6800 automatic
hematology analyzer

4.Sampling position adjustment

The correct sampling position
should be as follow :

Tube should
Aline with stay at the
each other center of
mechanical
hand center
 BF6500&BF6800 automatic
hematology analyzer

If the position is not like above picture ,it needs adjustment

as follow :
1.Put a rack with at least 2 tubes on the loader .
2. Then Enter the “service”—“Detect” —“Mechanical check
”—
“Barcode Check’’
Then the rack will move to the mechanical hand position .
Click “Barcode Check’’ again the rack will move to sampling
position
3. If the tube passed the correct position or can not reach
the correct position as above picture show ,we need to
adjust the micro switch as follow to make it correctly .
BF6500&BF6800 automatic
hematology analyzer

Adjust this micro switch

Loose this screw to

adjust it
 BF6500&BF6800 automatic
hematology analyzer

5.Mechanical hand adjustment

If the mechanical hand has excursion in forward and backward
direction as follow ,then the adjustment is necessary
 BF6500&BF6800 automatic
hematology analyzer

Excursion in
Excursion in
backward
forward
direction
direction
 BF6500&BF6800 automatic
hematology analyzer

1.Loose the screws as follow

Then adjust the position
of Mechanical hand to
fit the Requirement.
2.Fasten the screws again

Screw
Screw
 BF6500&BF6800 automatic
hematology analyzer

If the mechanical hand has excursion in horizontal direction as

follow ,then the adjustment is necessary

Excursion

Excursion
 BF6500&BF6800 automatic
hematology analyzer

Loose the screws

and make the
adjustment of
mechanical hand in
horizontal direction
 BF6500&BF6800 automatic
hematology analyzer

6.Preesure adjustment

Preesure Regulator

The required pressure is 100 ±5Mpa, it could be check in system

status
 BF6500&BF6800 automatic
hematology analyzer

Pressure adjust Regulator

Step 1 :exhaust the pressure in the

chamber
Step 2: lift up the regulator and rotate it in
clockwise direction to decrease the
pressure ,vice versa . Repeat this process
3-4time to make the pressure stable .

Exhaust Knob
 BF6500&BF6800 automatic
hematology analyzer

5.Maintenance
1 .Regular Maintenance
Daily Maintenance:
Rinsing Equipment should be carry out every 24 hours .
Click the Icon to run it

It could be also setup in setting –Auto cleaning setting

interface, you may choose 10—200 as interval number base on the
sample capacity per day .
 BF6500&BF6800 automatic
hematology analyzer

Weekly Maintenance:
If rinsing equipment is conducted everyday, the “Soaking with detergent
“ should be carry out every week .
Enter service –Maitain—soaking with detergent
Then run WBC Pool ,RBC pool ,DIFF pool one by one
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

Monthly Maintenance:
The rinse Swab should be carry out every month
Enter service –Maitain—rinse—rinse Swab
Then run rinse Swab
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

Every 3 Months Maintenance

Clean the Filter for waste valves

Open the left cover of analyzer ,find the valve 3
assembly and remove those 4 filters .
Open the filters and clean the inner side
Close them and fasten it to avoid any leakage
Fix them on valve 3 assembly again
 BF6500&BF6800 automatic
hematology analyzer

Necessary Maintenance
1.If live the analyzer vacantly more than 2 weeks ,the package clean
and empty should be carry out .

Replace all the reagent with distilled water

Enter service –Maitain—Empty —Package clean pipe
Then disconnect water
Then click package empty pipe
 BF6500&BF6800 automatic
hematology analyzer

2.If the analyzer give alarm as “aperture clog” please carry out
aperture as follow

•Enter service –Maitain—Aperture —Remove clog

•Zap
•Back Flush
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

3.If the analyzer does not startup for a long time ,please prime the
pipe before use

•Enter service –Maitain—Replace/Prime

•Prime FDO
•Prime FDT
•Prime SLS
•Prime Diluent
•Prime PK
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

4.Sheath Flow cell Maintenance

Sheath tubing maintenance needs to be carried out when the
instrument tests 7000 samples or it has been used for 3 month.
Flow Cell Soaking
a. Drain the liquid in 4-diff mix pool （ refer to user manual for
operation ） .
b. Pull out the tube 64 connected with valve SV1, and connect it
with waste container.
c. Pull out the tube 42 from connector, and connect with 5 ml
injector with detergent.
d. Push the injector gentlely, push out 4 ml detergent, push and
pull the injector repeatedly, soak for 25 mins, and then push out the
rest detergent.
e. Aspirate 5ml diluent with injector, and conduct rinsing according
to above method.
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

6.Upgrading
1. Control Board Upgrading
Control Board upgrading: Open service interface of BF-
6800 information management system, click 【 Servic
e 】 ---> 【 Status 】 ---> 【 System Version 】 , open c
ontrol panel interface, as figure shows:
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

Click upgrade, the following interface pops up, as figure shows.

 BF6500&BF6800 automatic
hematology analyzer

Click OK, select the control panel document need to be upgraded, the follo
wing interface pops up, as figure shows.
 BF6500&BF6800 automatic
hematology analyzer

Click Open, control panel start to upgrade, the following box pops up, as
figure 8-4 shows
 BF6500&BF6800 automatic
hematology analyzer

The following interface pops up after successful upgrading.

Click OK as figure 8-5 shows.
 BF6500&BF6800 automatic
hematology analyzer

2 .Software Upgrading
Note ： Exit the program before BF-6800 information management syste
m upgrading.
Click Upgrade.exe in program installation directory, as figure 8-6 shows.
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
BF6500&BF6800 automatic
hematology analyzer
 BF6500&BF6800 automatic
hematology analyzer

7.Troublshooting
1. 4 Diff scattergram does not appear after test or bad scattergram

Analysis :
1.Mixed sample can not reach flow cell
2. Not enough reagent reach 4 diff bath or expired reagent
3.Dirty or blocked
 BF6500&BF6800 automatic
hematology analyzer

Solution :

Blocked or low sensitivity valve may preve

nt the test syringe from aspirating enough mi
xed sample to the pipeline between SV27 a
nd SV24.

Disconnect the relative pipe connection then

activate the pressure pump , vacuum pump
or check with syringe manually to find the de
fective valve .
 BF6500&BF6800 automatic
hematology analyzer

The blocked sample injection probe m

ay preven the sample from passing throu
gh the flow cell .

Disconnect the relative pipe connection

and inject detergent to sample injection
probe manually to dissove the things insi
de the probe .
 BF6500&BF6800 automatic
hematology analyzer

The mixed sample may be discharged to va

cuum pipeline once the SV15 can not close w
ell .

The impurity inside the Diff bath may effe

ct the test result .
Soak it with Naclo 5% for 20min to dissove
the impurity in it .
 BF6500&BF6800 automatic
hematology analyzer

Thanks