M Y C 0 PL A S M A

· Also known as Pleuro-Pneumonia-Like Organisms (PPLO)

smallest bacteria (0 2M)

·
pathogenic .

· filtrable
through pores (bacterial filters (

· Lack cell wall

highly pleomorphic (au shapes and sizes (

cell
resistant to all wall
acting agents (lysozymes B-Lactams)
-

·
cell membrane contains sterols

·
Jumping joker of microbiology cell wall-nt and sterols +ht in cell membrane
-

·
Lack enzymes for proteins , patty acids and iterols synthesis
nutritionally fastidious . Sterols are essential growth factors
PPLO and PPLO broth
agar

·
Primary isolation from the specimen PPLO Broth

subculture

PPLO Agar activation

·
selecting agents in PPLO
proth/agar
Thallium acetate

Penicillin

·
Mycoplasma do not show turbidity in broth medium

· solid medium-colonies -

pried egg colonies/mulberry-shaped colonies

Diene's stain

demonstrates the colonies

MYCOPLASMA PNEUMONIAE

· Eaton's
agent strictly human pathogen
immunocompetent aerosol transmission
immunocompromised

secondary attack rates -

80 %

·
Pharyngitis ,
tracheobronchitis ,
otitis media , primary atypical pneumonia
(10 -
13 % )

most
·
Mycoplasma Pneumoniac common cause
of community-acquired
-

atypical pneumonia
.
 ·
Primary atypical pneumonia-walking Pneumonia or cold agglutinin
disease

X-Ray Chest-streaks interstitial infiltration

of
·

Specimens-sputum
·
,
bronchial
washing

·
Giemsa staining
-

poor sensitivity

· culture-I-2 weeks

NAATS sensitive method

most method most preferred
-

·
,

·
serology
Heterophile agglution test.

1 . Cold agglutination (aggutination at 4

°
C) ( > 1 : 32)

LIGM + human blood group O RBC's)

2
. Streptococcus MG Aest ( > 1 : 20)

cantibodies F Streptococcus
against a group
Specific anti-mycoplasma antibodies
cantibodies form very useful)
beyond the acute
stage -
not

Complications
·

Dermatologic -
rashes ,
Steven Johnson's syndrome
Neurological myelitis
meningitis encephalitis transverse
-

, ,

Hematological autoimmune
-

hemolytic anemia
, thrombocytopenia ,

Pancytopenia
hepatitis , glomerulonephritis , pericarditis , pancreatitis

· Treatment

Macrolides (azithromycin) choice

-

drug of
alternatives -

doxycycline , fluoroquinolones (levofloxacine maciflox ,

accines

malaise harsh
Q .

21-yr-old- +
Low-grade perer +
lough >
- 1 week

CXR

diffuse interstitial Pneumonia (left Cobes)

-
WBC -
normal

cold
agglutinins 1 : 64
-
-

-complement-filling antibodies
negative

-
Blood
-

Sputum culture
V normal oral flora
Macconrey

.
A
A DNA proble to the 16s ribosomal RNA
of an organism lacking a cell .
wall