BRIEF REPORT

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Template for preparing your Brief Report 63
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4 submission to PNAS using Overleaf 65
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6 Author Onea,c,1 , Author Twob,1,2 , and Author Threea 68
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This manuscript was compiled on May 22, 2023
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Please provide an abstract of no more than 250 words in a single paragraph. Abstracts should
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explain to the general reader the major contributions of the article. References in the abstract
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must be cited in full within the abstract itself and cited in the text.
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14 Keyword 1 | Keyword 2 | Keyword 3 | ... 76
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T
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his PNAS journal template is provided to help you write your work in the
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correct journal format. Instructions for use are provided below.
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Note: please start your introduction without including the word “Introduction”
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as a section heading (except for math articles in the Physical Sciences section); this
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heading is implied in the first paragraph.
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Guide to using this template on Overleaf 85
24 Please note that whilst this template provides a preview of the typeset manuscript 86
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for Authors. FT
for submission, to help in this preparation, it will not necessarily be the final
publication layout. For more detailed information please see the PNAS Information

If you have a question while using this template on Overleaf, please use the help
menu (“?”) on the top bar to search for help and tutorials. You can also contact the
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or feedback on the template.
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33 Author Affiliations. Include department, institution, and complete address, with the 95
34 ZIP/postal code, for each author. Use lower case letters to match authors with 96
35 institutions, as shown in the example. PNAS strongly encourages authors to supply 97
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39 ORCIDs on proofs. 101

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41 Submitting Manuscripts. All authors must submit their articles at PNAScentral. If 103
42 you are using Overleaf to write your article, you can use the “Submit to PNAS” 104
43 option in the top bar of the editor window. 105
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45 Format. Many authors find it useful to organize their manuscripts with the following 107
46 order of sections: title, author line and affiliations, keywords, abstract, introduction, 108
47 results, discussion, materials and methods, acknowledgments, and references. Other 109
48 orders and headings are permitted. 110
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50 Manuscript Length. Brief Reports are limited to 3 pages, which is approximately 112
51 1,600 words (including the manuscript text, title page, abstract, and figure legends), 113
52 and 15 references. Supporting information (SI) is limited to extended methods, 114
53 essential supporting datasets, and videos (no additional tables or figures). a b 115
Author affiliations: Affiliation One; Affiliation Two;
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Affiliation Three
55 References. References should be cited in numerical order as they appear in text; 117
56 this will be done automatically via bibtex, e.g. (1) and (2–5). All references cited 118
57 in the main text should be included in the main manuscript file. Please provide details of author contributions here. 119
58 Please declare any competing interests here. 120
59 Data Archival. PNAS must be able to archive the data essential to a published 1
A.O.(Author One) contributed equally to this work
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60 article. Where such archiving is not possible, deposition of data in public databases, with A.T. (Author Two) (remove if not applicable). 122
61 such as GenBank, ArrayExpress, Protein Data Bank, Unidata, and others outlined 2
To whom correspondence should be addressed. E- 123
62 in the Information for Authors, is acceptable. mail: author.twoemail.com 124

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125 Figures and tables should be labelled and referenced in 187
126 the standard way using the \label{} and \ref{} commands. 188
127 Figure 1 shows an example of how to insert a column-wide 189
128 figure. To insert a figure wider than one column, please 190
129 use the \begin{figure*}...\end{figure*} environment. 191
130 Figures wider than one column should be sized to 11.4 cm or 192
131 17.8 cm wide. Use \begin{SCfigure*}...\end{SCfigure*} 193
132 for a wide figure with side legends. 194
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134 Tables. Tables should be included in the main manuscript file 196
135 and should not be uploaded separately. 197
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Single column equations. Authors may use 1- or 2-column
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equations in their article, according to their preference.
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To allow an equation to span both columns, use the
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\begin{figure*}...\end{figure*} environment mentioned
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above for figures.
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Note that the use of the widetext environment for
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equations is not recommended, and should not be used.
143 Fig. 1. Placeholder image of a frog with a long example legend to show justification 205
144 setting. 206
Supporting Information (SI). Authors are limited to the
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Table 1. Comparison of the fitted potential energy surfaces and ab following types of SI: extended methods, datasets, videos,
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initio benchmark electronic energy calculations and 3D figures. Extended discussion is not permitted.
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Species CBS CV G3 SI Appendix. Supply extended materials and methods infor-
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1. Acetaldehyde
2. Vinyl alcohol
3. Hydroxyethylidene
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50.8 FT
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nomenclature for the TSs refers to the numbered species in the table.
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mation in a separate SI Appendix PDF file. Include SI movie
legends. SI will be published as provided by the authors; it
will not be edited or composed. Supplementary figures and
tables are not allowed.

SI Datasets. Supply .xlsx, .csv, .txt, .rtf, or .pdf files. This file
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Language-Editing Services. Prior to submission, authors who
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believe their manuscripts would benefit from professional 219
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editing are encouraged to use a language-editing service SI Movies. Supply Audio Video Interleave (avi), Quicktime 220
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(see list at https://www.pnas.org/author-center/language- (mov), Windows Media (wmv), animated GIF (gif), or MPEG 221
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editing). PNAS does not take responsibility for or endorse files and and include a brief legend for each movie in the main 222
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these services, and their use has no bearing on acceptance of manuscript file. All movies should be submitted at the desired 223
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a manuscript for publication. reproduction size and length. Movies should be no more than 224
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163 10 MB in size. 225

Digital Figures. EPS, high-resolution PDF, and PowerPoint
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are preferred formats for figures that will be used in the main
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manuscript. Authors may submit PRC or U3D files for 3D Materials and Methods 227
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images; these must be accompanied by 2D representations Please describe your materials and methods here. This can be more
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in TIFF, EPS, or high-resolution PDF format. Color images than one paragraph, and may contain subsections and equations
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must be in RGB (red, green, blue) mode. Include the font
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files for any text.
170 Subsection for Method. Example text for subsection. 232
Images must be provided at final size, preferably 1 column
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width (8.7cm). Figures wider than 1 column should be sized
172 ACKNOWLEDGMENTS. Please include your acknowledgments 234
to 11.4cm or 17.8cm wide. Numbers, letters, and symbols here, set in a single paragraph. Please do not include any
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should be no smaller than 6 points (2mm) and no larger than acknowledgments in the Supporting Information, or anywhere
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12 points (6mm) after reduction and must be consistent. else in the manuscript.
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178 1. M Belkin, P Niyogi, Using manifold stucture for partially labeled classification in Advances in 4. RJF Thomas, “Enamel defects, well-being and mortality in a medieval Danish village,” PhD 240
neural information processing systems. pp. 929–936 (2002). thesis, Pennsylvania State University, University Park, PA (2003).
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2. P Bérard, G Besson, S Gallot, Embedding riemannian manifolds by their heat kernel. Geom. 5. TM Toolan, “Advances in Sliding Window Subspace Tracking,” M.Sc. thesis, University of
180 & Funct. Analysis GAFA 4, 373–398 (1994). Rhode Island, Kingston, RI (2005). 242
181 3. RR Coifman, et al., Geometric diffusions as a tool for harmonic analysis and structure 243
definition of data: Diffusion maps. Proc. Natl. Acad. Sci. United States Am. 102, 7426–7431
182 (2005). 244
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(x + y) = (x + y)(x + y)
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Lead author last name et al. PNAS — May 22, 2023 — vol. XXX — no. XX — 3