Quantitative Amino Acids - Mass Kit

Quant�tat�ve Analys�s of Am�no Ac�ds �n Plasma, Ur�ne and CSF Samples

Problems in specific metabolic pathways in the human body cause metabolic disorders. Metabolic disorders are usually
inherited diseases and result from decreased or complete absence of one or more enzymes' activity. These activity
disorders cause some metabolites to accumulate in the body and cause toxic effects, and subsequently, organ failure
and systemic diseases.

Identification of metabolic diseases is carried out by routine newborn screening methods. Permanent damage to
metabolic diseases can be prevented with special diet treatments applied following the diagnosis of metabolic disease.
Continuous monitoring of the plasma concentrations of metabolic products is required to monitor the effectiveness of
the treatment to be administered to patients.

Trimaris Quantitative Amino Acids LC-MS/MS Kit allows quantitative monitoring of free amino acid concentrations in
samples such as plasma, urine and cerebrospinal fluid of patients treated for metabolic diseases. Components such as
calibrator set, reagents and mobile phases required for analysis are provided with the kit.

KAT.012.1 / 29.12.2021 / Rev0

Sample Preparation

P�pette 100 µL of pat�ent sample (or

Add 100 μL of Reagent 1. M�x by vortex�ng
cal�brator, control) �nto a glass sample
for 15 seconds.
preparat�on tube.

Add 200 μL of Reagent 3. M�x by vortex�ng

for 15 seconds. Incubate at room Add 200 μL of Reagent 2. M�x by vortex�ng
temperature for 3 m�nutes. for 15 seconds.

Add 200 μL of Reagent 4. M�x by vortex�ng

M�x aga�n by vortex�ng for 15 seconds.
for 15 seconds. Centr�fuge for 5 m�nutes at
Incubate at room temperature for 1 m�nute.
8.000 rpm

Transfer the clear supernatant to the

autosampler v�al after centr�fugat�on. Inject
0.2 μL sample to the LC-MS / MS system.

Sample Chromatogram LC-MS/MS Parametreleri

Dev�ce SHIMADZU 8040 LC-MS/MS
Analys�s Method LC-MS/MS, ESI pos�t�ve �on�zat�on
DL Temperature 250 °C
Heat�ng Block Temp. 400 °C
Dry�ng Gas 15 L/dk
Nebul�zer Gas 3 L/dk
Q1-Q3 Resolut�on Un�t
Column Tr�mar�s Quant�tat�ve Am�no Ac�ds
LC-MS/MS Analyt�cal Column
(BR130030AC)
Analyt�cal Column
Temperature 30 °C
Flow Rate 0,25 mL/m�n
Sample Volume 0.2 µL
T�me %A %B
00:00 65 35
12:00 35 65
Grad�ent 12:01 5 95
16:00 5 95
16:01 65 35
Performance Data
No Analyte Symbol Recovery L�m�t of Detect�on L�m�t of Quant�tat�on L�near�ty Range Intra-assay Inter-assay
(LOD) (LOQ) Prec�s�on Prec�s�on
1 Alan�ne ALA % 95.3 0.10 μmol/L 0.30 μmol/L 0.30 – 2500 μmol/L % 2.16 % 4.30
2 Arg�n�ne ARG % 96.0 0.05 μmol/L 0.15 μmol/L 0.15 – 1500 μmol/L % 4.87 % 7.13
3 Asparag�ne ASN % 93.3 0.60 μmol/L 1.80 μmol/L 1.80 – 1500 μmol/L % 5.72 % 11.42
4 Aspart�c Ac�d ASP %108.5 0.05 μmol/L 0.15 μmol/L 0.15 – 1200 μmol/L % 5.26 % 8.57
5 C�trull�ne CIT % 94.2 0.06 μmol/L 0.18 μmol/L 0.18 – 1000 μmol/L % 4.96 % 6.69
6 Glutam�ne GLN % 96.9 0.34 μmol/L 1.05 μmol/L 1.05 – 2500 μmol/L % 3.25 % 9.78
7 Glutam�c Ac�d GLU % 103.5 0.15 μmol/L 0.45 μmol/L 0.45 – 2000 μmol/L % 3.03 % 8.26
8 Gyc�ne GLY % 93.9 0.09 μmol/L 0.27 μmol/L 0.27 – 2500 μmol/L % 4.49 % 6.72
9 H�st�d�ne HIS % 95.4 0.10 μmol/L 0.30 μmol/L 0.30 – 2500 μmol/L % 1.82 % 5.09
10 Leuc�ne LEU % 97.5 0.07 μmol/L 0.21 μmol/L 0.21 – 1500 μmol/L % 5.13 % 5.99
11 Isoleuc�ne ILEU % 92.0 0.20 μmol/L 0.66 μmol/L 0.66 – 1500 μmol/L % 5.33 % 8.89
12 Lys�ne LYS % 94.9 0.12 μmol/L 0.36 μmol/L 0.36 – 2500 μmol/L % 4.80 % 9.92
13 Meth�on�ne MET % 91.6 0.09 μmol/L 0.27 μmol/L 0.27 – 1500 μmol/L % 4.01 % 4.46
14 Orn�th�ne ORN % 93.1 0.08 μmol/L 0.24 μmol/L 0.24 – 1500 μmol/L % 1.97 % 6.92
15 Phenylalan�ne PHE % 96.2 0.17 μmol/L 0.51 μmol/L 0.51 – 2500 μmol/L % 4.57 % 6.22
16 Prol�ne PRO % 94.4 0.03 μmol/L 0.09 μmol/L 0.09 – 1500 μmol/L % 4.84 % 9.06
17 Ser�ne SER % 92.4 0.44 μmol/L 1.31 μmol/L 1.31 – 1500 μmol/L % 4.37 % 6.74
18 Threon�ne THR % 94.3 0.15 μmol/L 0.45 μmol/L 0.45 – 1500 μmol/L % 3.39 % 7.67
19 Tryptophan TRP % 95.0 0.04 μmol/L 0.12 μmol/L 0.12 – 1500 μmol/L % 3.86 % 8.32
20 Tyros�ne TYR % 94.2 0.08 μmol/L 0.24 μmol/L 0.24 – 1500 μmol/L % 4.99 % 7.30
21 Val�ne VAL % 96.2 0.11 μmol/L 0.33 μmol/L 0.33 – 2500 μmol/L % 4.65 % 5.30
22 Alphaam�noad�p�c Ac�d AAA % 92.4 0.16 μmol/L 0.48 μmol/L 0.48 – 1500 μmol/L % 5.51 % 9.55
23 Arg�n�nosucc�n�c Ac�d ARGSUC % 90.5 0.08 μmol/L 0.24 μmol/L 0.24 – 1500 μmol/L % 4.69 % 10.95
24 Alphaam�nobutyr�c Ac�d ABA % 89.8 0.03 μmol/L 0.09 μmol/L 0.09 – 1500 μmol/L % 6.02 % 7.54
25 Betaam�nobutyr�c Ac�d BAIB % 93.2 0.75 μmol/L 2.25 μmol/L 2.25 – 1500 μmol/L % 2.61 % 9.68
26 Gammaam�nobutyr�c Ac�d GABA % 95.4 0.47 μmol/L 1.42 μmol/L 1.42 – 1500 μmol/L % 2.78 % 7.87
27 Beta alan�ne BALA % 96.5 0.05 μmol/L 0.15 μmol/L 0.15 – 1500 μmol/L % 5.95 % 6.36
28 Sarcos�ne SAR % 91.6 0.38 μmol/L 1.13 μmol/L 1.13 – 1500 μmol/L % 7.55 % 10.43
29 Cystath�on�ne CTH % 98.2 0.04 μmol/L 0.12 μmol/L 0.12 – 1500 μmol/L % 7.06 % 8.61
30 Th�aprol�ne THPR % 94.6 0.15 μmol/L 0.45 μmol/L 0.45 – 1500 μmol/L % 5.60 % 7.10
31 1-methylh�st�d�ne 1-MEHIS % 92.6 0.06 μmol/L 0.18 μmol/L 0.18 – 1500 μmol/L % 5.02 % 12.57
32 3-methylh�st�d�ne 3-MEHIS % 94.1 0.15 μmol/L 0.45 μmol/L 0.45 – 1500 μmol/L % 3.41 % 9.67
33 Hydroxylys�ne HYL % 97.8 0.25 μmol/L 0.75 μmol/L 0.75 – 1500 μmol/L % 3.46 % 7.97
34 Hydroxyprol�ne HYP % 100.5 0.03 μmol/L 0.09 μmol/L 0.09 – 1500 μmol/L % 3.39 % 6.92
35 Cyst�ne C-C % 106.1 0.07 μmol/L 0.21 μmol/L 0.21 – 1500 μmol/L % 4.17 % 11.72
36 Homocyst�ne HC-CH % 102.3 0.06 μmol/L 0.18 μmol/L 0.18 – 1500 μmol/L % 4.53 % 7.40
37 H�stam�ne HISTA % 95.8 0.10 μmol/L 0.30 μmol/L 0.30 – 1500 μmol/L % 4.10 % 5.88
38 Etanolam�ne EA % 93.5 0.58 μmol/L 1.75 μmol/L 1.75 – 1500 μmol/L % 5.08 % 9.23
39 Phosphoetanolam�ne PEA % 88.6 0.10 μmol/L 2.25 μmol/L 2.25 – 1500 μmol/L % 4.78 % 7.17
40 Phosphoser�ne PSER % 89.4 0.60 μmol/L 1.80 μmol/L 1.80 – 1500 μmol/L % 3.81 % 5.73
41 Taur�ne TAU %111.4 0.82 μmol/L 2.46 μmol/L 2.46 – 1500 μmol/L % 6.62 % 13.13
42 5-hydroxytryptophan 5OHTRP % 94.7 0.13 μmol/L 0.39 μmol/L 0.39 – 1500 μmol/L % 2.74 % 8.96
43 Carnos�ne* CAR % 96.2 0.10 μmol/L 0.30 μmol/L 0.30 – 1500 μmol/L % 3.45 % 7.35
44 Anser�ne* ANS % 94.5 0.12 μmol/L 0.36 μmol/L 0.36 – 1500 μmol/L % 3.46 % 9.15
45 Alphaam�nop�mel�c ac�d* APA % 96.7 0.25 μmol/L 0.75 μmol/L 0.75 – 1500 μmol/L % 3.61 % 6.86
46 Seroton�n* SRTN % 91.4 0.15 μmol/L 0.45 μmol/L 0.45 – 1500 μmol/L % 5.18 % 7.34

* Relevant am�no ac�ds are added to the analys�s l�st upon request. Cal�brator mater�al conta�n�ng these am�no ac�ds must be ordered separately.

Product Code Product Name Tests

BR130030 Quant�tat�ve Am�no Ac�ds LC-MS/MS K�t 200 Tests

BR130030AC Quant�tat�ve Am�no Ac�ds LC-MS/MS Analyt�cal Column
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