ANALYTICAL

SMARTCHEM 450 NORME

METHOD

W-E1-18 Sc450 HR
RANGE :

1 – 20 mg/L NH4 C17016

C17017

0.8 – 16 mg/L N-NH4

AMMONIA
WATER
KIT AMS FLUID N° DOC
01
 S U M M A R Y

1. PRINCIPLE .................................................................................................................................................... 3
2. REAGENTS .................................................................................................................................................... 3
R1 Reagent H2O ................................................................................................................................................. 3
R2 Reagent NHA ................................................................................................................................................ 3
R3 Reagent NHB ................................................................................................................................................ 3
3. PARAMETERS .............................................................................................................................................. 4
4. PERFORMANCES ......................................................................................................................................... 5
Calibration ........................................................................................................................................................... 5
Repeatability ....................................................................................................................................................... 5

CONFIDENTIEL
AMS ALLIANCE
10 avenue Charles de Gaulle
Ce document est strictement confidential.
95740 Frépillon, France
Tel. : +33 1 34 18 71 10
www.amsalliance.com
DOCUMENT UPDATE 03 08 2016
 1. PRINCIPLE

Ammonia determination is based on a coloured complex between NH4+ ion, salicylate and chlorine in
basic conditions. The pH at the colorimeter cell must be near 12,6. Colorimetric measures are performed
at 660 nm.

2. REAGENTS

R1 Reagent H2O
Ready to use reagent

R2 Reagent NHA
Ready to use reagent

R3 Reagent NHB
Ready to use reagent

Standards:

Calibration can be done from a stock solution at 20 mg / L NH4 in distilled water.

CODE
DESIGNATION REFERENCE
COMPOSANT
NHA A-08-A 600 C17016

NHB A-09-A 600 C17017

3
 3. PARAMETERS

General
Method Water - range ammonia 1 - 20 mg/L
Reaction type Point final
Direction Up
Filter 660
decimals 3

Liquids, volumes and times

Solution Reagent name Predilution ration Volume (µL) Time (s) Incubation time (s) Sample blank (SB)
Sample 1/1 60
Reagent 1 H2O 230 45 ☐

Reagent 2 NHA 150 75 / 

Reagent 3 NHB 100 632 ☐
Diluent H2O

Calibration
Calibration type Linear curve Point Concentration (µg/L) Concentration (%)
Use RBL for calculation Yes 1 0 0
RBL Replicates 3 2 0.4 2
RBL liquid H2O 3 1 5
4 3 15
Stock liquid name Ammonia 20 mg/L 5 10 50
Diluent name H2O 6 15 75
Force zero crossing No 7 20 100
Prediluted No 8 / /

4
 4. PERFORMANCES

Calibration

Repeatability

5
Measurement Range: The test is developed to determine the nitrite concentration of 1 to 20
mg / L (NH4) according to AMS programming.

Accuracy: Under the test conditions described above, the accuracy of the measurement is 5% on
a control solution

Test procedure: For programming on a PLC, programming is available on request.

Manual use: Consult us for the test protocol.

Storage instruction and stability of reagents: The reagents are stable until the expiration
date indicated, if stored at 2-8 ° C.
Caution: Do not swallow. Avoid contact with skin and mucous membranes.

Quality control: Not available