BA-A-S120Mini

FULLY AUTO
BIOCHEMISTRY ANALYZER

Version 2023.07.01
Basic parameter

a. 24+5 positions for sample, 1 position for calibration, 1 position for quality control, 1
position for emergency. The total volume of the sample cups is 850 μL
b. 27 double reagent positions, outer track is NO.1-NO.27, bottle volume is 8ml. Inner
track is NO.28-NO.54, bottle volume is 24ml. NO.27 is system position, please add
pure water to the bottle, cannot set as reagent position
c. 81 cuvettes, 9 cuvettes/group, 9 groups totally
d. Wavelength：340 405 450 500 546 578 630 670nm
e. Methodology : Two-point、End-point、Rate
f. Power supply: 50Hz AC100-240V
g. Power: 150W
h. Dimension: 605mm(L)*350mm(W)*330mm(H)
i. Net weight: 22kg

Testing
★ One sample can apply for up to 20 test items
★ Don't add reagent more than 2/3 of reagent bottle
★ Put pure water at No.27 Reagent position → for probe liquid level detection

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★ Check that the reagents and samples are properly placed before starting the test
★ Please ensure that the sample is sufficient to meet the test requirements. The test
results are not accurate when the total volume of serum in the sample cup is less than
300 μL, sample tube test volume can‘t less than 1.5ml.
★ If the machine detects a lack sample/reagent, result is * . *. User need reapply.
★ here will be a liquid level induction error warning when the machine is starting up.
Restart under the condition that the outlet water of the cleaning pool is normal. Set
to enter the none liquid level detection mode at this time: Not detect the volume of
samples and reagents, so please add enough reagents and samples to ensure the
accuracy of test results

1. Normal Test
a. Select sample number, ID number do not need to be modified
b. Select item combination、test item singly
c. Select the testing times(The default is 1 time), when testing repeatability we can
choose only one item
d. Press “Enter”, application is successful
e. Press “Start” , starting the test
f. ★ When you apply for the test after running out of 24 sample positions, you will
automatically jump to no. 1 to reorder, and the results of the previous test will be
automatically covered. Therefore, only 29(24 sample cup+5 tube) sample tests can
be started at a time.

2. QC test
a. Press QC, the system defaults quality control position is No. 26 (QC position)
b. Select the quality control test item
c. Press “Enter”, application is successful
d. Press “Start” , starting the test

3. Calibration test
a. Press “CAL”, the single point calibration is defaulted by No.25 position(CAL), and
the multi point calibration is defaulted by No.1 to No.5 positions
b. Set item parameters, select calibration item
c. Press “Enter" , “CAL” turn to blue, application is successful

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 d. Press “Start" , starting the test
e. The system automatically updates the F value of the item parameters after
calibration test is completed, the machine automatically prints the F value of the
calibration item
f. ★ In order to ensure the reliability of the calibration results, no operation is
allowed during the calibration test
g. ★ Single-point and multi-point calibration can not be applied for and started at
the same time, multi-point calibration can only apply for and start one item at a
time, single-point calibration can apply for and start more than one item at a time

4. Emergency test
a. Press “ST”, system defaults ST position is No.27(ST position)
b. Select ST item
c. Press “Enter”, application is successful
d. Press “Start" , starting the test

5. Eliminate
a. The tests that have been applied(blue) cannot be deleted
b. The sample that has been finished(green) can be deleted, press the sample
position to delete

6. Pause and resume

a. During the test, we can press “pause” key to pause test
b. After being paused, the machine will stop adding sample, but will continue collect
the photoelectricity, so please resume as soon as possible or restart the machine
c. ★ Press “pause” again to resume test
d. ★ Test results with incomplete sample addition are automatically re-tested at the
end of the test after resume
e. Do not resume test after pause, the machine will end the test after test whole
circle of the last complete test

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7. Stop
a. In an emergency, press “Stop” key to stop the machine
b. ★ When press “Stop” key, the machine stops all actions immediately, and the
unfinished tests are all invalid and cannot be resumed
c. ★ When you need to retest after stopping, you need to turn off the machine then
restart

8. View results

a. Press “S” to view results

b. Press Modify key to modify results, press Print key to print results
c. Press “Curve” to see the reaction curve
d. Press “QC” to view QC results, press “curve” to check QC chart
e. Press No.25 position’s result to check calibration test results, press “curve” to see
the reaction curve
f. The machine will print the result automatically after a sample test is completed,
you can also print results you want manually

9. Water blank test

a. After placing the new cuvettes for the first time, water blank test must be carried
out to ensure the consistency of the water blank data
b. After changing cuvettes, water blank test must be carried out to ensure the

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 consistency of the water blank test data
c. Water blank should be tested regularly during the continuous use of the same
cuvettes in order to eliminate the interference of the cuvettes aging to the water
blank. The best test period of the water blank is 1 time/day (after turn on the
machine, a water blank test before the test), Or the user should adjust the water
blank test cycle automatically according to the using frequency of the cuvettes
d. Ensure that the cuvettes are new cuvettes or washed cuvettes before the water
blank test
e. Press the water blank key for five seconds. The machine automatically tests the
water blank. After the test is finished, stop the machine and update the water
blank automatically. The water blank test process is: suction - cleaning once -
adding pure water - test the water blank - two times suction – stop

10. Replace cuvettes

a. The cuvettes is a semi-permanent cuvettes, and the new cuvettes needs to be
replaced when the frequency of use exceeds 100 times or reaches three months.
The user can also adjust the cuvettes replacing period according to the service
condition of the machine
b. Fit the partition plate, reagent/sample tray in turn, then turn the machine on,
press the replacing cuvettes key for 5 seconds after the machine passes the QC
and the machine defaults that cuvettes are all cleaned
c. ★ In order to ensure the accuracy of water blank, please test the water blank after
replacing cuvettes
d. ★ Please do not do this operation during the test
e. ★ The used cuvettes should be regarded as contaminated substance. Attention
should be paid to the corresponding protection measures when replacing them

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Setting
1. Item parameters setting

a. Press “set” key——Input password to setting interface

b. Select mode: Press to select. Touch screen mode/Computer mode/Duplex
Communication modeTouch screen mode: Select when using touch screen
software Computer mode: Select when using PC software Duplex Communication
mode: Select when using Duplex LIS system
c. Printer status——Print ON/OFF
d. Select calibration mode: 1 calibration/3 calibration
1 calibration: Select when perform single point calibration
3 calibration: Select when perform multi point calibration
e. Password: For close system use only
f. Item setting:
Item ID: ID number, Item: Item name
M.W: Main wavelength; S.W: Sub wavelength
S.Vol: Sample volume, Maximum:25ul Minimum:3ul
R1 Vol: R1 volume, Maximum:255ul
R2 Vol: R2 volume
Anly Method: Analysis method
Cal. Method: Calibration method
*S.Point: Start point Maximum:15
*E.Point: End point Maximum:25

Factor: Input manually or obtained after calibration

b.Factor: Input manually or obtained after calibration
Conc: Calibrator's concentration
Range: Result range

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 : Test type（Colorimetric/Blood/Turbidimetry）

: Copy Parameter setting

g. Service: Maintenance use only
h. Print: print setting
i. Save parameter: Click save → Click "Yes" → Click "Print list" → Click "Save" → Click
Test List → Click "Save"

2. Adding rule
The first period add R1, the Sixth period add S, the 13th add R2, maximum 25 period, 30
seconds/period
Incubation time → Incubation point: Incubation time(s)/30
For example: Incubate 300 seconds after added S, incubation point:300/30=10
Note:(Double reagent: Calculate incubation time after added R2 only, “R1+S” incubation time is
fixed time and does not need to be calculated and cannot be changed)

A: End-point
1. Single reagent End-point:
a. S.point: 3~5 (set any point)
b. E.point: 6+Incubation point, For example: Incubate 420s, E.point:6+14=20

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 Set: S.point=5, E.point=20

2. Double reagent End-point:

a. S.point:8~12(any point)
b. E.point: 13+incubation point For example: Incubate 330s,
theoretical E.point:13+11=24

Set: S.point=12, E.point=24

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d. Immune turbidimetry (if requires S.point is after added R2)
• S.point:14
• E.point: 14+incubation point For example:Incubate 300s,
E.point:14+10=24
•
Set: S.point=14, E.point=24

B:Kinetic & two-point Kinetic

Reading time / 30 =Reading point
For example: Reading 3 minutes, Reading point: 180/30=6
Note: Kinetic&2-point Kinetic if reading point ≤ 4, calculate by 4
1. Single reagent Kinetic&Two-point Kinetic
a. S.point: 6+Incubation time(s)/30, For example: Incubate 240s after added,
S.point: 6+8=14
b. E.point: 6+Incubation point+Reading point, For example:
Incubate 60s after added S, then reading 2.5 minutes abs.
E.point: 6+8+5=19

Set: S.point=14, E.point=19

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2. Double reagent Kinetic&Two-point Kinetic
a. S.point:13+ Incubation time(s)/30 For example: Incubate 30s, S.point:
13+1=14
b. E.point: 13+Incubation point+Reading point For example:
Incubate 60s, then reading 2 minutes abs. E.point: 13+2+4=19

Set: S.point=14, E.point=19

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3. Item combination setting
a. Press “set” key——Input password to setting interface
b. Press “Item setting”
c. Select item combination button
d. Select or deselect the item
e. Press “Enter” key to save
f. ★ A item combination contains twenty items at the most
g. Press “modify” and then press “item combonation” to modify name

4. Debug command

a. Press “set”——press “Service”

b. Press the corresponding command and the machine runs the corresponding
action
c. ★ To ensure the safety of the probe, check whether the needle is in the highest
initial position before rotating the probe
d. ★ When the probe is rotated to a position, the probe should be rotated to the
initial position when the next rotation is necessary
e. Adjust the probe position: Press ”POS setting” ——The adjustment interface is as
follow:

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5. Date and time
Press the corresponding column of date and time to modify

Daily maintenance and matters needing attention

a. Check whether the pure water is enough before turning on the machine，
whether the waste liquid bucket is empty，and clean the pure water bucket
regularly to ensure the water quality
b. Whether printing paper is enough
c. Check whether the reagent is enough and the position is correct before testing
d. The reagent bottle has a dead volume of less than 1 ml，this part should be
discarded when adding reagents，reagents of different batches cannot be used in
combination. Reagents should be re-calibrated after changing the batch number
e. Re-calibrate after modifying the item parameter
f. est to see if the sample centrifuges meet the test requirements to prevent
clogging. The sample in the sample cup should be filled
g. Can’t add samples or reagents while testing，prevent personal injur y and
machine malfunction

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 h. When the test results are completed, the machine should not be shut down while
it is cleaning, wait for the cleaning till complete
i. Refrigerate the reagent immediately after the test to ensure the stability of the
reagent
j. Clean the probe tip and cleaning pool with alcohol ever y day to ensure
cleanliness
k. All substances in contact with the machine are considered infectious，pay
attention to personal safety during operation

Correlation parameters and calculation methods

1. Test period：
a. The 1st period add R1
b. The 6th period add S
c. The 13th period add R2

d. Thirty seconds a period

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e. 25 periods totally
f. The machine will clean the used cuvettes without adding sample in the period
g. The machine will clean all used cuvettes after the reaction is over until all the cups
have been cleaned
h. If turn off the machine in an abnormal condition, the machine will automatically look
for clean cuvettes for testing in the next test. If there is no usable cuvettes, it will clean
the cuvettes first till there are enough usable cuvettes to test

2. Calculation methods
a. End-point method：（end-point N）
b. Reaction range = end-point absorbance（（N+N-1）/2） – star t point
absorbance
c. 2-point method：Reaction range = end-point absorbance － starting point
absorbance /60
d. Rate method：Reaction range=The rate of change in absorbance per minute
between the starting point and the end point
e. If set the dual wavelength，the main wavelength minus the secondary wavelength
and then calculate

3. Calibration formula
C（concentration）=Reaction amplitude * F value + b value
F value and B value are got by calibration, or inputting the values in the item
parameters

Serial communication protocol

a. Serial interface：COM232
b. Baud rate：19200
c. Check bit：even parity check
d. Start bit：1bit
e. Stop bit：1bit

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 Data Data IP
Header Command Data Bytes Data description
length checksum checksum

0x0001 2 Sample ID

0x01 1 Test type

0x01 1 Sample Pos.

0x01 1 Result 1 Item ID

0x5348 0x54 0x00F6 0x0001 0x0001 0x0001 1 Result 1 Data

High 4 Byte Error Type, Low 4
0x01 1 Byte Decimal

0x01 1 Result 20 item ID

0x0001 2 Result 20 data

0x0001 1 Data Checksum

Fault and alarm

Code Fault description Fault causes Solution

1.Reagent/sample position Check the placement of reagents and

3 Collision fault incorrect or bottle cap samples. If they still appear, please contact
unopened. 2. Sensor fault. with maintenance engineer

Syringe initial position 1. Sensor fault. 2. Syringe Restart，If they still appear, please contact
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sensor fault mechanical fault. with maintenance engineer

1. Check whether the pure water bucket

Liquid level detection 1.No pure water in the
7 has enough pure water. If they still appear,
fault cleaning pool 3.Sensor fault
please contact with maintenance engineer

Reaction tray Code 1. Sensor fault. 2. Reaction tray Restart，If they still appear, please contact
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disc fault mechanical fault. with maintenance engineer

Reaction tray initial 1. Sensor fault. 2. Reaction tray Restart，If they still appear, please contact
14
position sensor fault mechanical fault. with maintenance engineer

Check water tank and Water-in pump,

1.No water in water tank.
16 Lack of water If they still appear, please contact with
2.Water-in pump off work
maintenance engineer

Machine can’t detect inner-

Internal-walls pump Restart，if they still appear, please contact
19 wall valve signal during self-
fault with maintenance engineer
check

1.Machine can’t detect

Restart，if they still appear, please contact
20 Collision sensor fault collision signal during self-
with maintenance engineer
check

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Code Fault description Fault causes Solution

Machine can’t detect External

External walls pump Restart，if they still appear, please contact
21 -walls valve signal during self-
fault with maintenance engineer
check

1. lack of reagents or reagent

Place reagent correct. If they still appear,
22 Lack of R2 position incorrect. 2.Sensor
please contact with maintenance engineer
fault

1.Machine can’t detect

Waste-liquid pump Restart，if they still appear, please contact
23 Waste-liquid pump signal
fault with maintenance engineer
during self-check

Check if the shell is opened properly.

1.Shell of machine opened
28 Opening shell fault Restart，if they still appear, please contact
incorrect
with maintenance engineer

Lack of Water blank Machine cannot read tests of Retest water blank test, if they still appear,
29
tests water black please contact with maintenance engineer

In photoelectric collection, Enter debug software, check photoelectric

30 Photoelectricity fault photoelectric data exceeds the collection, if they still appear, please
upper and lower limits contact with maintenance engineer

1. lack of reagents or reagent

Place reagent correct. If they still appear,
32 Lack of R1 position incorrect. 2.Sensor
please contact with maintenance engineer
fault

Replace the reagent according to the

Insufficient number of The remaining reagent cannot
33 procedure. If they still appear, please
reagent tests meet the requested test
contact with maintenance engineer

The sampling probe does not

1. Check reagent bottle or sample cup. 2.
Liquid level detection drop to the specified height,
40 Check whether the Liquid level induction
fault but the liquid level has been
light is always on
detected

1. lack of samples or sample

Place sample correct. If they still appear,
42 Lack of sample position incorrect. 2.Sensor
please contact with maintenance engineer
fault

When the reaction

tray rotates, the It will be corrected automatically in normal
1.Sensor fault. 2. Washing
81 washing mechanism operation. If it occurs frequently, please
mechanism mechanical fault
is not in the initial contact the maintenance engineer
position

When the reaction

It will be corrected automatically in normal
tray rotates, the 1.Sensor fault. 2. Sampling
82 operation. If it occurs frequently, please
sampling probe is not probe mechanical fault
contact the maintenance engineer
in the initial positionz

Washing head sensor 1.Sensor fault. 2. Washing Restart，if they still appear, please contact
83
fault head mechanical fault with maintenance engineer

Probe up-down 1.Sensor fault. 2. Probe up- Restart，if they still appear, please contact
84
sensor fault down mechanical fault with maintenance engineer

Probe rotate sensor 1.Sensor fault. 2. Probe rotate Restart，if they still appear, please contact
85
fault mechanical fault with maintenance engineer

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