13.

EFFECT OF SOLVENT ON ABSORPTION MAXIMA OF PARACETEMOL

AIM: To determine the effect of solvent on absorption maxima

APPRATUS: Volumetric flask, pipette, beaker, glass rod

CHEMICALS: Paracetemol, distilled water, methanol

PRINCIPLE:

λ max is a constant value in a particular solvent as the solvent system varies, the λ max also
varies,The shift in absorption band depends on the polarity of solvent and the solute. For π -> π*
transition, the π* state is more polar and stabilized more in polar solvent relative to nonpolar,
thus in going from nonpolar to polar solvent there is a red shift or bathochromic shift (increase in
λmax). For n -
> π* transition, the n state is much more easily stabilized by polar solvent (H-bonds and
association), so in going from nonpolar to polar solvent there is a blue shift or hypsochromic shift
(decrease in λmax).

PROCEDURE:

Weigh accurately 100mg of Paracetemol powder and add distilled water to make it 100ml in a
volumetric flask, shake well and scan the above solution to get spectrum against the distilled water
as blank. Similarly make the paracetamol solution by using methanol as solvent. Scan the above
solution to get spectrum against the distilled methanol as blank. Finally overlap both the spectra
and observe the shift in λ max

REPORT:

The absorption maxima using methanol was found at higher wavelength.

Questions:

1.Discuss the effect of solvent on absorption maxima.

2.Explain about Hypsochromic shift and Bathochromic shift.

3.Write the chemicals required for effect of solvent on Absorption maxima.

4.Write the principle for Beer-Lamberts law.

5. Explain about different electronic transitions involved in Absorption spectroscopy.