Bellerry C.

Bernante
BSMT 3C
Eyepiece Also known as ocular, where viewers can see through the specimen
Body Tube Long tube, that connects both eyepiece and objective lenses
Nosepiece Objective lenses remain attached to it
It can rotate to adjust the objective lens
Objective lens Contain different types of objective lens (10x, 40x, 100x)
Located below nosepiece
Lenses are closest to the specimen
Flat metal platform located above the condenser and below the objective lens.
Stage Clips Above the stage
Holds the slide
Base Supports all the components of the microscope
Arm It connects the body tube and base of the microscope
Illuminator It is the light source of compound microscopes
Has a low voltage bulb, located below the stage
Iris diaphragm It adjusts the amount of light that reaches the specimen
Diaphragm It is a five holed disk placed under the stage
Fine Can be used to adjust the focus and increase the detail of the specimen
adjustment
Coarse Focuses on the specimen
Adjustment

QUESTIONS:

1. What does it mean when a microscope is said to be parfocal?

The microscope is said to be parfocal when, after you adjust the focus, the image will remain
approximately in focus even if you change the magnification. Switching magnification from low
power/scanning high power the focus will remain.

2. Which objective focuses closest to the slide?

The objective lens that focuses closest to the slide is the immersion oil objective (100x)

3. What controls the amount of light reaching the ocular lens?

The Iris Diaphragm controls the amount of light that reaches the ocular lens.

4. Name two ways in which you can enhance the resolving power.

- Switching to a higher aperture lens.

- Decrease in the amount of light being used.
5. What advantage does the LPO have over OIO?

The advantages of using a Low power objective is that when you are viewing a fungi or algae under the
microscope the field of view is clearer and crisper, than using the Oil immersion objective.

6. What is the purpose of adding cedar wood oil when using OIO and why?

The purpose of adding cedar wood oil when using Oil immersion objective lens is to avoid the refraction
at the air-glass interface.

7. What is the working distance of each of the objectives?

The working distance of each objective, is the vertical distance from the objective's front lens to the
closest surface of the specimen when the specimen is sharply focused. It is the space you have to get a
specimen in under the lens and still get a focus.

8. What is the correlation with the iris diaphragm, condenser and working distance to the three objective
lenses?

The correlation between the iris diaphragm, condenser and working distance to the three objective lens
is, the condenser has an iris diaphragm below it which controls the beam of light being focused onto the
specimen. They work together to control the specimen’s illumination and focus.

9. What is the numerical aperture of each objective?

The numerical aperture of each objective lens is a measure of its ability to gather light and resolve fine
specimen detail at a fixed object distance.