Quiz-3, CHE 362 (Date:21-10-21, Time – 30 min)

1. Some microbial species may produce different products when grown under different
nutritional & environmental conditions. In the same line of thought, under which of
the following conditions, ethanol production by Saccharomyces cerevisiae seen?
a. Under aerobic conditions without glucose in culture media
b. Under anaerobic conditions without glucose in culture media
c. Under aerobic conditions with glucose in culture media
d. Both b & c.

2. Energy is stored in cells in the form of _____________

a. ATP
b. GTP
c. NADH
d. FAD𝐻2

3. How many molecules of Pyruvate will be synthesized from 5 molecules of glucose

during glycolysis?
a. 8 molecules
b. 10 molecules
c. 5 molecules
d. 19 molecules

4. What is the necessary reaction step for Krebs cycle to begin?

a. Acylation of CoA by Pyruvate
b. Reduction of Pyruvate to lactate
c. Addition of C𝑂2 to Pyruvate
d. None of the above

5. For each glucose molecule being catabolized, how many molecules of C𝑂2 is
released?
a. 3 molecules
b. 6 molecules
c. 4 molecules
d. 8 molecules

6. “TCA cycle is an important biochemical reaction involved in glucose catabolism, but

it can also contribute to biosynthesis of amino acids.”
a. True
b. False
 7. “Glycolysis happens in both aerobic & anaerobic organisms, but TCA cycle occurs in
aerobic organisms.”
a. True
b. False

8. Complete the following table for aerobic breakdown of 1 glucose molecule:

NADH FAD𝐻2 ATP Total ATP
Glycolysis 2 - (i) 6
Oxidative (ii) - - 6
decarboxylation
of pyruvate
TCA cycle 6 2 2 (iii)
Total (iv)

a. (i) 4, (ii) 6, (iii) 10, (iv) 22

b. (i) 2, (ii) 2, (iii) 24, (iv) 36
c. (i) 2, (ii) 6, (iii) 21, (iv) 33
d. (i) 2, (ii) 2, (iii) 18, (iv) 30

9. In Electron transport chain, the final electron acceptor is

a. Oxygen
b. Cytochrome b
c. Quinone
d. NADH

10. The following reaction can be categorized as ______________

Glutamic acid + oxaloacetic acid →→ alpha-keto glutaric acid + aspartic acid
a. Deamination reaction
b. Oxidation reaction
c. Transamination reaction
d. Isomerization reaction

11. Succinate and a-ketoglutarate produced during the TCA cycle are used as precursors
for the synthesis of certain amino acids.
a. True
b. False
12. Purines/pyrimidines are broken down into simpler products like __________
a. Ammonia & C𝑂2
b. Urea & acetic acid
c. Amino acids
d. None of the above

13. Aromatic hydrocarbons like Benzene enters TCA cycle after it gets converted into an
intermediate compound. What is the name of the compound?
a. Citric acid
b. Cathecol
c. Oxaloacetate
d. Pyruvate

14. Which of the following are biosynthetic pathways?

a. HMP pathway
b. Gluconeogenesis
c. Both a & b
d. None of the above

15. The intermediates in HMP pathway can be utilized in various other biochemical
pathways. Which of the following compounds can be derived from this pathway?
a. Coenzyme A
b. Pyruvic acid
c. Tyrosine
d. All of the above

16. Gluconeogenesis is basically_______

a. Reverse of Glycolysis
b. Reverse of Krebs Cycle
c. Reverse of Fermentation
d. None of the above

17. The starting compound for fatty acid synthesis is ____________

a. Acetyl-CoA
b. Ribose-5-phosphate
c. Glyceraldehyde-3-phosphate
d. Pyruvate
18. The process of ATP synthesis from Electron transport chain is called __________
a. Fermentation
b. Oxidation phosphorylation
c. Respiration
d. None of the above

19. In autotrophs, C𝑂2 fixation for biosynthesis of carbohydrates is mediated by which

biochemical pathway?
a. Light reaction
b. Calvin cycle
c. Both a & b
d. None of the above

20. Which of the following reactions would be called fermentation reaction?

a. Production of Lactic acid
b. Production of ethanol
c. Production of tryptophan
d. Both a & b

21. Cell number can be determined using which of the following methods?
a. Hemocytometer
b. Viable cell counts
c. Particle counter
d. All of the above

22. We can determine the cell mass concentration indirectly by using luciferin.
a. True
b. False

23. During cell growth, long lag phase may be observed due to___________
a. The use of very aged inoculum
b. Presence of toxic by-products
c. The cells used as the inoculum are in exponential phase
d. All of the above reasons
24. You wish to synthesize an expensive Antibiotic using a bacteria species & to make
profit, you desire to increase your yield. Which of the following strategy will you try
to implement for achieving this goal?
a. Try to have long lag phase than exponential phase
b. Try to have long stationary phase than exponential phase
c. Do nothing & let the cells grow at own rate.
d. Try both a & b as it will help to achieve our goal quicker

25. What is cryptic growth?

a. Cells grow due to the presence of another carbon source when primary carbon
source has exhausted
b. Cells grow on the lysis products of lysed cells
c. Cells grow under limited nutrient conditions
d. Cells grow under limited Oxygen conditions