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FACULTY OF APPLIED SCIENCES

KUALA LUMPUR CAMPUS

BABS1223 ECOLOGY AND BIODIVERSITY

COURSEWORK : PRACTICAL REPORT _1_

RBS 1 (Sem 1/ Group 1) MARKS:


CLASS:

PREPARED BY,

NAME STUDENT ID

1. Ng Wing Yet 23WLR07861

2. Lee Chung Kit 23WLR02236

3. Caitlin Chia Sook Yan 23WLR07852

4. Soma Watanabe 23WLR0293I

5. Evelyn Samantha Carlos 23WLR02624

Name of Lecturer: Ms. Selvi A/P Chellappan

Date of Submission: 23/7/2023

Practical: 1 Constructing a Phylogenetic Tree


RUBRIC FOR ASSESSING LAB REPORTS
Factor/Section Beginning/Incomplete/Poor Developing/Acceptable Accomplished/Good Excellent/Exemplary Score

Introductio No much description, very Basic description of topic Good description pf Comprehensive
n& little background information but still missing some the topic. Introduction description of the topic
Objectives provided or information is major points such as is nearly complete, and well written. Provides
incorrect. (0 – 2) objectives. (3 – 5) missing some minor all the necessary
(10%) info. (6 – 8) background principles for
the experiment (9 - 10)

Methodology No description or very little Missing some important Important experimental All experimental details
(10%) description of steps. experimental details. List details are covered, are covered. List all the
Incomplete and missing all the steps in a some minor missing steps in a detailed,
several important sequential but not n details. List all the steps sequential order
experimental details. (0 – order. (3 – 5) accordingly. (6 – 8) that are easily
2) followed. (9 – 10)

Results (20%) Very little/ inappropriate Basic description with Good description and Excellent description
description. Minimal data simple observation with observation. Logical and and tabulation of
presented. sufficient data presented. well adequate organized results. Well drawn
Figures/Graphs/Tables Adequate and logical graph/table/figures. graph/table/figures
contain errors or poorly organized (11-15) with proper labelling.
constructed. (0 – 5) graphs/table/ figures. (6 – 10) (16 – 20)

Discussion Poor explanation. Very Some of the results have Almost all the results Excellent interpretation of
(30%) incomplete or incorrect been correctly interpreted have been correctly results with use of
interpretation of results. (0 – and discussed. Insufficient interpreted and literature. All the
8) explanation or discussed. Good use of important data and trends
understanding of results. literature to explain the comparison have been
(9 – 15) results. Minor interpreted correctly and
improvement needed. discussed. (23 – 30)
(16 – 22)

Conclusion No conclusion or Stating very All important and Stating an excellent


(10%) missing the important general/simple logical conclusion. conclusion that matches
points (0-2) conclusion with lack of Missing minor points all experimental
understanding (3 – 5) (6 – 8) analysis.
Good understanding.
(9 -10)

Content & Not organized / poor Some organization with Adequate organization Excellent organization
Style (10%) organization. Poor sentence minor format error. with good formatting but with very good
construction with many Improved sentences with could be improved. Very formatting. Well written
grammatical errors. No minor minor and good grammar.
references. (0 – 2) grammatical error. grammatical error. Good references. ( 9
Inadequate references. Adequate references. (6 -10)
(3 – 5) -8)

Hands-on (10%) Didn’t participation during Participation is less Participation is good. Good participation and
practical classes. No during practical. Less Good teamwork active. Good teamwork
teamwork during group teamwork during group during group with group. Follow
activities. Never activities. activities but never pay instruction. (9 – 10)
follow the instruction. (0 - 2) Follow instruction but attention in following
make mistakes. (3 – 5) instruction. (6 – 8)
1.0 Objectives
The purpose of this lab report is to acquire the skill and knowledge to identify organisms.
Furthermore, learn how to compose a phylogenetic tree and to understand the classification of
different organisms.

2.0 Introduction
BD Editors (2020) define a phylogeny as a hypothetical connection between groups of creatures
that are being compared. A phylogeny can be represented by a phylogenetic tree. A phylogenetic
tree is a branching diagram that shows the evolutionary links between different animals or
groups of organisms (shown in Figure 1.0 below). It is also known as an evolutionary tree or a
tree of life. It demonstrates the evolutionary history and pattern of descent from a common
ancestor of taxonomic units or species (Sourav Bio, 2023).

Figure 2.1 Phylogenetic Tree


In this experiment, the phylogenetic tree used to visualise the phylogeny of the group of animals
is a Cladogram. A cladogram is a style of tree diagram that uses common features or
characteristics to show the evolutionary connections among a collection of animals (shown in
Figure 1.1 below) (Sourav Bio, 2023). The root is the centre stem of a cladogram that represents
the common ancestor of all groups spreading from it. A cladogram is made up of branching lines
that lead to a clade, which is a group of creatures that share a possible ancestor. The spots where
the lines intersect are known as nodes and are the common ancestors (Helmenstine,2020).

Cladograms are used by scientists to make hypotheses and pose queries on the evolutionary
relationships between various species. Scientists are able compare different phylogenies of the
same groupings of organisms by giving weight to derived traits and recognising ancestral
characteristics (BD Editors, 2017).
Figure 2.2 Cladogram depicting the phylogeny of a group of vertebrates

3.0 Results

Table 3.1 Different characteristics between a leopard and a domestic cat

Social Weight Size Number of


behaviour teeth

Leopard Solitary The average The average size is 24-26 baby


weight is 50 around 210 cm in teeth and 32
to 90 kg. head-to-body length, permanent
with around 90 cm teeth
long tails.

Domestic cat Sociable The average The average size is 26 baby teeth
weight is 4 around 46 cm in and 30
to 5 kg. head-to-body length, permanent
with around 30 cm teeth
long tails.
Table 3.2 Animals with backbone
1 2 3 4 5 6 7 8

Lungs Ovipara Beak Tail Endothermic Carnivore Marsupials Horn

1 Panda 1 1 1

2 Kiwi 1 1 1 1

3 Cat 1 1 1 1

4 Bison 1 1 1 1

5 Sea 1 1 1
turtle

6 Dog 1 1 1 1

7 Lion 1 1 1 1

8 Frog 1 1 1

9 Duck 1 1 1 1 1 1

10 Fish 1 1 1

11 Snake 1 1 1 1

12 Koala 1 1 1 1

13 Zebra 1 1 1

14 Penguin 1 1 1 1 1 1

15 Walrus 1 1 1 1

16 Bull 1 1 1 1
Table 3.3 Animals without backbone
9 10 11 12 13 14 15 16 17
Breathe Suction More than 4 3 segment Spin
Gills air Whisker cups Tentacles Shell legs body web
17 Spider 1 1 1 1
19 Cockroach 1 1 1
20 Moth 1
21 Jellyfish
22 Octopus 1 1 1
23 Shrimp 1 1
Hermit 1 1
24 crab 1
25 Sea star 1 1

4.0 Discussion
The selected animals are distinguished and identified firstly through the presence of backbone.
The animals are then differentiated by their breathing method and physical traits such as
presence of gills, whisker, shell and suction cups. Animals are classified based on morphological
and developmental characteristics and distinguished using ecological, behavioral and molecular
data.

5.0 Conclusion
The purpose of this lab report is to acquire the skill and knowledge to identify organisms.
Furthermore, learn how to compose a phylogenetic tree and to understand the classification of
different organisms. The method of distinguishing animals by their physical traits and habitat
was applied for making a phylogenetic tree, which displays evolutionary relationships among
them. Although some common features are not obvious, our knowledge and skills to identify
animals were enhanced through this practical section.

4.0 Discussion
Based
Figure 3.3 Phylogenetic tree

4.0 Discussion

One of the specific characteristics that can be used to separate a leopard and a domestic
cat is social behaviour. A Leopard is solitary whereas a domestic cat is rather sociable. Besides,
they can also be differentiated through their weight. For example, leopards weigh 50 to 90 kg
averagely while the average weight of domestic cats is 4 to 5 kg. In addition, leopards have a
much larger size compared to domestic cats. Specifically, the average size of leopards is around
210 cm long from head to body with around 90 cm long tails and the average size of domestic
cats is around 46 cm in head-to-body length with around 30 cm long tails. Lastly, leopards differ
from domestic cats in the number of teeth. In particular, leopards have 24 to 26 baby teeth and 32
permanent teeth whereas domestic cats have 26 baby teeth and 30 permanent teeth.

There are several ways to distinguish and identify selected animals. Firstly, the selected
animals are distinguished and identified based on morphology. For example, kiwi, duck and
penguin have beaks. Bison and bulls have horns. Moreover, hermit crabs have shells. Octopus
and sea star have suction cups. Secondly, they are also distinguished and identified by anatomy.
Such as, all selected animals with backbone have lungs except fish. Thirdly, they can be
distinguished and identified based on their behaviour. For example, spiders can spin webs. All
selected animals with backbone are endothermic animals, except sea turtle, frog, fish and snake.
For animals without backbone, only spiders and cockroaches can breathe air. Finally, selected
animals can be distinguished and identified by their modes of reproduction. For instance, kiwi,
sea turtle, frog, duck, fish, snake and penguin are oviparous animals.

Animals are divided into categories based on shared features and evolutionary links.
Taxonomy is the study of the classification of organisms. There are several main factors that
used to classify animals. Firstly, the classification of animals is based on morphology. Body
shape, size, bone structure, and the presence or absence of certain features, such as wings, fins
and limbs are all important considerations in animal taxonomy. Physical characteristics can assist
in discovering similarities and differences between species. Secondly, the other factor is
anatomy. Internal features and organ systems are important factors in animal taxonomy. The
arrangement and complexity of organs, skeletal features, and other anatomical details aid
biologists in classifying animals. Thirdly, one of the factors is embryonic similarities. The study
of embryonic development sheds light on the evolutionary links between animals. Early
developmental similarities and variances can aid in determining evolutionary links. Moreover,
classification of animals can also be based on the genetic similarities (DNA). Animal
classification has been transformed by molecular data such as DNA sequences and genetic
research. Genetic similarities and differences can indicate evolutionary links and provide more
precise insights into species relatedness. Furthermore, animals can be classified by their
evolutionary relationship, which includes fossils. Extinct creatures and their links to extant
organisms are revealed by fossil evidence. Palaeontologists utilise fossils to piece together the
evolutionary history of different animal groups. Lastly, one of the factors is about the
biochemical similarities. To identify ancestry, scientists examine nucleotide sequences in DNA
and proteins in various animals. If the species are closely related, they will have comparable
nucleotide sequences in their DNA and amino acid arrangements in proteins (Oswald Richards,
n.d). The more similar the amino acid sequence, the more closely related the organisms are.

5.0 References

Biologydictionary.net Editors. (2020, June 11). Phylogeny. Biology dictionary. Retrieved from
https://biologydictionary.net/phylogeny/

Biologydictionary.net Editors. (2017, January 13). Cladogram. Biology dictionary. Retrieved


from https://biologydictionary.net/cladogram/

Sourav Bio. (2023, May 22). Difference between cladogram and phylogenetic tree.
Microbiology.note. Retrieved from
https://microbiologynote.com/difference-between-cladogram-and-phylogenetic-tree/

Anne Marie Helmestine. (2020, January 10). What Is a Cladogram? Definition and Examples.
ThoughtCo. Retrieved from
https://www.thoughtco.com/cladogram-definition-and-examples-4778452#:~:text=A%20cladogr
am%20is%20a%20type,groups%20branching%20off%20from%20it.

Oswald, R.(n.d). Evidence of Evolution. SlidePlayer. Retrieved from


https://slideplayer.com/slide/7784393/

Leopard | Description, Habitat, & Facts | Britannica (2023, Jun 20). Retrieved from
https://www.britannica.com/animal/leopard

Cat - Wikipedia (2023, July 14). Retrieved from


https://en.wikipedia.org/wiki/Cat#:~:text=It%20averages%20about%2046%20cm,(9%20and%20
11%20lb)

Clouded Leopard Teeth: Everything You Need To Know - AZ Animals (a-z-animals.com) (2022,
Jan 10). Retrieved from
https://a-z-animals.com/blog/clouded-leopard-teeth-everything-you-need-to-know/
Interesting Facts About Cat Teeth | PetMD (2019, 2 Dec). Retrieved from
https://www.petmd.com/cat/care/9-interesting-facts-about-cat-teeth

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