Transpiration: The Effects of Wind and Light on Solanum lycopersicum Katelyn Stapler Biology 156 March 9, 2011

Lab Partners: Magan Domingue Mary Lee Amanda Parham

a tomato plant. Introduction 2 . make transpiration rates faster. not wind. The hypothesis for the experiment is that direct light on the plant will make transpiration occur more rapidly. Results from the experiment prove that light.Abstract Wind and light were used to test the rate of transpiration of Solanum lycopersicum. Transpiration occurred more rapidly in the controled experiment instead of the lighted experiment. Transpiration is the loss of water from the stomata of a plant’s leaves.

The purpose of the experiment is to find out how quickly transpiration would occur with and without light or wind. Obtain a stem from a tomato plant (Sloanum lycopersicum). and humidity can affect transpiration. Materials and Methods A transpirometer apparatus. wind. Open the clamp that is on the line leading to the pipet and 3 . transpiration increases at first and after signs of water loss from the stomata transpiration rate slowly decreases. Submerge the stem in water and attach it to a water-filled tube connected to a pipet (Vodopich and Moore 2010). light. Unsubmerge the stem and tubing and wrap in a papertowel and place the stem into open clamp. Lightly clamp the stem to keep from stopping transpiration from occuring. In higher temperatures transpiration increases due to the rate of change of saturation pressure within the plant (Jarvis and Morrison 1981). Clamp off the micropipet using a spring clamp.Transpiration is the loss of water from the Stomata of plant leaves (Vodopich and Moore 2010). Make sure there are no air bubbles within rubbr tubing and that there is no water on the leaves before starting. Then set up the transpirometer apparatus by opening the screw valve below the reservoir and flushing out the system. The hypothesis for the experiment was that the tomato plant or Solanum lycopersicum would absorb more water with light instead of wind since light causes photosynthesis to occur more rapidly. a tomato plant stem. In the presence of wind. a fan and a lamp were needed for the experiment. The rate of transpiration decreases only when satruation pressure decreases (Raven and Singer 2010). Environmental conditions such as Carbon dioxide levels.

temp: 21C) Light (avg. temp: 22C) Time (min) 0 5 10 15 0 5 10 15 Distance (mm) 2. Table1. Results In Table 1 the overall class results are shown for wind and light.open the screw valve on the reservoir only enough to let water drop from the tip of the pipet. 45x10-5 and the transpiration rate for wind was 8.1mm 3.9mm 4. and tempurature for the light experiment.5mm 6.61x10-6. the transpiration of light was 9.4mm 2. Record data. Record the data every five minutes for fifteen minutes.3mm 1. Overall Class Final Results for the Light and Wind Experiments Trial Light Wind Class Transpiration Rate Average 9.1mm 5.1mm . Table 2 and Figure 1 show the results of an individual group instead of the overall class.4mm Transpiration Rates of the Control Experiment and the Light Experiment 7 6 5 4 4 . Control vs. every five minutes for fifteen minutes. Light Transpiration rates according to temperature differences Experiment Control (avg. As transpiration occurs water will slowly be drained from the pipet.45x10-5 8.61x10-6 Table 2. then close the screw valve. Then repeat the experiment with a fan that is placed 200mm away from the plant or with a lamp that is placed 200mm away from the plant.

Compared with the class data. The reason behind this was believed to be that during the controlled experiment the group did not start measuring and keeping track of time as soon as the experiment started. Light Transpiration Rate Discussion The experimental data collected by the group proves that the hypothesis is wrong in that light does not make transpiration occur more rapidly. transpiration was already well underway when the first measurements were being recorded. the experimental data that the group collected was off. the experimental data collected by the class proves this hypothesis to be correct. 5 . The class experimental data proves that transpiration occurs more rapidly when there is direct light to stimulate the plant.Distance In Milimeters Time in Minutes Figure 1. Thus. The hypothesis that light makes transpiration occur more rapidly is infact correct. Control vs. Yet.

"The Control of Transpiration and Photosynthesis by the Stomata. NY: Cambridge UP. 6 . Cambridge." Stomatal Physiology. and Morrison. 247-56. 1981. P. Print.Literature Cited Jarvis. J.

Mason. Biology. Singer. Kenneth A. and Susan R. IA: McGraw-Hill. George B.. 2010. Biology Laboratory Manual. and Randy Moore. 2010. Johnson. Peter H. 7 . Losos. 9th ed.Raven. 9th ed. Vodopich. Dubuque.. William C Brown Pub. Print. Darrell S. Jonathan B. Print.

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