IMagic Service Manual

Intellectual property rights
This service manual and the corresponding products of intellectual property belong to the
Shenzhen iCubio Biochemistry technology Co., Ltd. (hereinafter referred to as "iCubio Company").
Shenzhen iCubio Biochemistry technology Co., Ltd has the right. Without the written consent of
iCubio any individual or organization may not copy, modify, or translate any part of this manual.
Statement
iCubio Corporation has the final explanation on the service.
Meet the requirements of all of the following circumstances, iCubio’s response was that the safety,
reliability and performance responsibility, namely:
1 An assembly operation, expansion, re-adjust, improve and repair should be carried out by the
person with qualification approved by iCubio;
2 The electrical equipment in line with national standards;
3 Products in accordance with the instruction manual for operation.
Maintenance Service
Free Services range:
Products that meet the iCubio warranty products under the scope of the Ordinance can enjoy free
services.
Service fee range:
1 Where the regulations beyond the scope of iCubio's warranty provisions of products, iCubio will
implement fee-based services;
2 Even during the warranty period, due to the following causes of product needs repair: The
man-made damage; improper use; voltage exceeds the specified product range; irresistible
natural disasters; replacement of accessories without iCubio's allow, supplies, or by non-iCubio
authorized person maintenance machine.

Returns procedure
The company returned to iCubio, please follow these steps:
1. To obtain the right to return: contact with iCubio's customer service department, tell the iCubio
product serial number, the serial number is marked on the outside shipping box, if the serial
number cannot be identified, return not be accepted. Please specify product model, serial number,
brief the reason for the return.
2. Shipping: Products shipped to iCubio's service, users must bear the shipping charge (including
customs fees).
Service Manual release date:2015-07,Version: V.2.3
Customer service
Shenzhen iCubio Biochemistry Technology Co.,Ltd
Customer service department
Add: 11/F, Building A, Qiaode Science & Technology Park, No.7 Road, Hi-Tech Industry,
Guangming new district, Shenzhen, China.
Zip： 518106
24-hour hotline: 4006350600
Tel： +86 755-26610931
Fax： +86 755-26610893, 755-61658199

Website： www.icubio.com
Content
Chapter 1 System Specification .................................................................................. 1
Chapter 2 System Profile ............................................................................................ 2
2.1 System Structure： .................................................................................. 2
2.2 Reaction Tray System .............................................................................. 4
2.2.1 Cuvettes ............................................................................................. 5
2.2.2 Light Source System .......................................................................... 5
2.2.3 Filter System ...................................................................................... 6
2.2.4 Heating System.................................................................................. 6
2.2.5 Photoelectricity Conversion System................................................... 7
2.2.6 Machinery Motion &Transfer System ................................................. 7
2.3 Reagent System....................................................................................... 8
2.3.1 Reagent Rack and Sample Rack ....................................................... 8
2.3.2 Probe ................................................................................................. 9
2.3.3 Ram Pump ......................................................................................... 9
2.4 Mixing System ........................................................................................ 10
2.5 Wash System ......................................................................................... 11
2.6 Control System....................................................................................... 11
2.7 System Connection ................................................................................ 12
Chapter 3 System Installation ................................................................................... 13
3.1 Check before Installation ........................................................................ 13
3.2 Installation Requirement......................................................................... 13
3.2.1 Space Requirement ......................................................................... 13
3.2.2 Environment Requirement ............................................................... 13
3.2.3 Power Supply Requirement ............................................................. 14
3.2.4 Temperature and Humidity Requirement ......................................... 14
3.2.5 Water Supply and Dewatering Requirement .................................... 14
3.3 Installation .............................................................................................. 15
3.3.1 Water Bucket Connect ..................................................................... 15
3.3.2 Connect RS232 Serial Cable and Power Line ................................. 15
Chapter 4 Software ................................................................................................... 16
4.1 Software Installation ............................................................................... 16
4.1.1 iChemMini Installation ...................................................................... 16
4.2 Installation Detail .................................................................................... 18
4.2.1 Backup Data Before upgrade ........................................................... 18
4.2.2 Start Install Program ........................................................................ 19
4.2.3 Start Software to Set Parameters. ................................................... 19
4.3 Maintenance........................................................................................... 19
4.3.1 Daily Maintenance ........................................................................... 19
4.3.2 Device Check ................................................................................... 20
4.3.3 Absorbance Test .............................................................................. 20
Chapter 5 System Maintenance ................................................................................ 21
5.1 Daily maintenance.................................................................................. 21
5.2 Weekly Maintenance .............................................................................. 21
5.3 Monthly Maintenance ............................................................................. 21
5.4 Half Year Maintenance ........................................................................... 22
Chapter 6 Service ..................................................................................................... 22
6.1 Reagent and Sample System Failure ..................................................... 22
6.1.1 Reagent Rack .................................................................................. 22
6.1.2 Sample Rack.................................................................................... 23
6.1.3 Refrigeration Assembly .................................................................... 23
6.1.4 Reagent System Fault Point ............................................................ 24
6.1.5 Sample System Failure .................................................................... 24
6.2 Probe System Failure ............................................................................. 24
6.2.1 Probe Drive Assembly ..................................................................... 24
6.2.2 Probe Drive principle........................................................................ 25
6.2.3 Probe System Fault Point ................................................................ 27
6.3 Filter System Failure .............................................................................. 28
6.3.1 Filter System .................................................................................... 28
6.3.2 Filter System Drive Principle ............................................................ 28
6.3.3 Filter wheel system fault point .......................................................... 28
6.4 Reaction Tray System ............................................................................ 29
6.4.1 Reaction Tray Drive Assembly ......................................................... 29
6.4.2 Heat Prevention Assembly ............................................................... 31
6.4.3 Reaction Tray System Fault Point.................................................... 31
6.5 Photoelectricity Detect System............................................................... 32
6.5.1 Detect Principle ................................................................................ 32
6.5.2 Matters Need Attention of Installation .............................................. 33
6.5.3 Photoelectricity Detect System Fault Points .................................... 33
6.6 Liquid Flow Path..................................................................................... 33
6.6.1 Valve ................................................................................................ 36
6.6.2 Disc Pump........................................................................................ 37
6.6.3 Ram Pump ....................................................................................... 37
6.7 Wash Arm System Fault Point ............................................................... 39
6.7.1 Wash Arm Drive Principle ................................................................ 39
6.7.2 Wash Arm System Fault Point ......................................................... 40
6.8 PCBA Replace ....................................................................................... 40
6.8.1 Main Board....................................................................................... 42
6.8.2 Motor Driving Board ......................................................................... 46
6.8.3 Signal Adapt Board .......................................................................... 53
6.8.4 Signal Accept Board (J1) ................................................................. 54
6.8.5 X Axis Motor Interface Board ........................................................... 55
6.8.6 Y Axis Motor Interface...................................................................... 57
6.8.7 Heat & Refrigerate Board ................................................................. 59
6.8.8 Z Axis Interface Board ..................................................................... 63
6.8.9 Liquid Level Detect Board ................................................................ 64
Chapter 7 Debug....................................................................................................... 65
7.1 Debugging of Optical Assembly ............................................................. 65
7.1.1. Confirm Filter wheel Assembly Parameters ..................................... 65
7.1.2. Filter wheel assembly movement confirmation ................................ 66
7.1.3. Fix Lamp Assembly.......................................................................... 67
7.1.4. Debugging of Filter Wheel Specific Position .................................... 68
7.1.5. Debugging of Filter Wheel Gain ....................................................... 70
7.1.6. Lamp Information ............................................................................. 71
7.2 Debugging of Reaction Tray Assembly .................................................. 71
7.2.1 Confirmation of Reaction Tray Assembly Parameters ..................... 72
7.2.2 Debugging of Reaction Tray optocoupler position ........................... 72
7.2.3 Debugging of Reaction Tray Specific Position ................................. 73
7.2.4 Debugging of reaction tray offset ..................................................... 76
7.2.5 Debugging of reaction tray temperature........................................... 77
7.3 Debugging of Ram Pump Assembly ...................................................... 77
7.3.1 Confirmation of Ram Pump Assembly Parameters .......................... 77
7.3.2 Confirmation of Ram Pump Assembly Motion Function ................... 78
7.4 Debugging of Wash Arm Assembly ........................................................ 79
7.4.1 Confirmation of Wash Arm Assembly other Parameters .................. 79
7.4.2 Confirmation of Wash Arm Assembly Motion Function .................... 80
7.4.3 Fix Wash Arm Assembly .................................................................. 80
7.4.4 Debugging of Wash Arm Assembly specific Position ....................... 82
7.5 Debugging of sampling arm ................................................................... 83
7.5.1 Confirmation of sampling arm Parameters ....................................... 84
7.5.2 Debugging of optocoupler shelter position ....................................... 85
7.5.3 Probe Special Position Adjustment .................................................. 89
7.6 Liquid Path Debugging ......................................................................... 104
7.6.1 Pipe line Confirmation .................................................................... 104
7.6.2 Adjustment for Clean cell liquid path .............................................. 105
7.6.3 Wash Arm liquid Path Adjustment.................................................. 106
7.6.4 Deion water- waste liquid level detection ....................................... 106
Chapter 8 Analysis Method ..................................................................................... 107
8.1 End-point method ...................................................................................... 107
8.2 Two-point method ...................................................................................... 107
8.3 Rate method (kinetics) ............................................................................... 107
Attachment 1: The hardware fault code table..................................................... 108
Attachment 2: The software fault code table ...................................................... 112
Attachment 3: Trouble shooting table ................................................................ 114
Chapter 1 System Specification
Test Speed 100～120 Test/H
Configuration Host + PC
System Discrete, Multi-channel, Multi-item
Test Method End Point, Kinetic, Fixed Time
Test Item Defined by software; Request one by one, Profile, calculate item and manual
item.
Sample Volume 2～100uL，0.1μl step up
Sample Rack Only for iMagic series
Sample Position 22, position can be defined by operator.
Probe Liquid level detect and vertical collide protect.
Probe Wash Both inside and outside
Reagent 1 180～350μl,0.1μl step up
Reagent 2 0~200μl,0.1μl step up
Reagent Rack Only for iMagic series
Online Reagent 50, suit for 20ml iCubio reagent bottle
Mix Sample probe mixing after dispense sample and reagent.
Light Source 6V/10W halogen tungsten lamp.
Spectral Method filters ,then spectroscope
Half Wave Width ≤12nm
Wave Length 340nm，405nm，450nm，510nm，546nm，578nm，630nm，700nm
AD Range 0～3.5A.
Cuvettes Light length 5.8mm, repeat use.
Reaction Volume 200～650μl
Reaction Temperature 37±0.1℃
Data Processing Store, output data and diagram, calculate among different items.
Calibrate Method Linear( One point, two point and multi point), Nonlinear)
Q.C. Westgard multi-rule，L-J chart
Dimensions Length×Width×Height: 498X463X434mm
Weight ＜40kg
Operation System Windows XP，Windows7，Windows 8
Interface RS-232
Printer Decided by Windows system
Power Source AC 110/220V, 50/60Hz
Power ≤300W
Work Environment Temperature 15℃～30℃, fluctuate <±2℃/H
Relatively Humidity 35% ～ 80%, Nonfrost.
Water Consume 3L/H
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Chapter 2 System Profile
iMagic series is discrete auto-wash chemistry analyzer, 100-120test/hour, with reagent

refrigeration, sample and reagent dispense, mixing after sample and reagent dispense,
absorbance test and cuvettes auto wash.
Reagent rack for reagent store with refrigeration function, temperature range is 4℃ to 15℃,
sample rack for sample store. 48 cuvettes in reaction tray with auto wash function. Cuvettes can
be replaced separately, and all the cuvettes are reusable. Reaction tray is adopted metal heating,
so the temperature rise fast, suit for STAT test. iMagic series is using single probe for reagent and
sample dispense, and also mixing after dispense sample and reagent. Probe auto wash both
inside and outside, avoid of cross-contamination between different samples and items.
2.1 System Structure：
Shell
Reagent and sample

dispense system
Reaction tray
Auto wash system
Probe
Reaction tray
Sample rack
Reagent rack
Figure 2-1 Front view
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Host switch
RS232
Power switch
Fuse protector
Power socket
Figure 2-2 Back interface
Deionized water sensor

Waste Liquid sensor
Detergent (Optional)
Deionized water 1 Deionized water 2
Waste liquid 1 Liquid waste 2
Figure 2-3 The side of liquid road interface(with auto cleaning)
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Deionized water Waste liquid sensor
sensor
Waste liquid 1
Deionized water 2
Figure 2-4 The side of liquid road interface(without auto cleaning)
Note: the positions of the liquid pipeline from up to down and from left to right are Liquid level
sensor, Liquid level sensor, water injection, water injection, waste liquid, and waste liquid.
2.2 Reaction Tray System
Reaction tray system including cuvettes, light source, filter system, heating system,
photoelectricity conversion system, machinery motion and transmit system.
Figure 2-5 Reaction tray
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2.2.1 Cuvettes
Cuvettes are used for sample and reagent reaction and colorimetric. It’s made of special
material or quartz glass with good transmission performance. Quantity of cuvettes is directly affects
analysis effect. Reaction tray rotates at constant speed, probe dispense reagent and sample into
cuvettes and mix while it stops rotation. While cuvettes bypass photoelectricity detection circuit, it
tests absorbance of sample in the cuvettes. iMagic series has 48 cuvettes, which are auto washing.
Cuvettes placed in metal rack directly, which can be replaced one by one.
Figure 2-6 Cuvettes
2.2.2 Light Source System
Figure 2-7 Light source system
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Light source system supplies compound light for test. The halogen lamp of 6V/10W provides
the light from 300nm to 800nm. Lamp are installed and replaced in assembly.
2.2.3 Filter System
Filter wheel
Figure2-8 Filter system

Filter system is used for transfer light from halogen lamp to filter. There are 8 filters in the filter
wheel to get different wave length light for test by transferring the light from light sources to
monochromatic light.
2.2.4 Heating System
Figure 2-9 Heating system
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iMagic series adopts metal heating method, which install a circle of orange heating belt in the
opposite of heating groove. The temperature rise fast and transfer even to whole tray.
Heat-sensitive sensor to make sure the temperature is 37℃. While temperature is higher than
37℃, heating belt stops heat. Always keep the temperature at 37℃, and make sure the reaction
of reagent and sample in cuvettes is stable.
2.2.5 Photoelectricity Conversion System
Signal processing board
Figure2-10 Photoelectricity conversion system
Photoelectricity conversion system converse the monochromatic light after the filter to electricity
signal, and then calculate absorbance. The monochromatic light from 340nm, 405nm, 450nm,
510nm, 546nm, 578nm, 630nm, 700nm accepted by signal adapt board converse to analog
signal, then converse to digital signal transfer to main board.
2.2.6 Machinery Motion &Transfer System
Step motor
Optocouple
Signal processing
Synchronization
belt
Synchronization
belt gear
Figure2-11 Machinary Motion Transfer System
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Machinery Motion Transfer System controls the rotation of reaction tray for dispensing of reagent,
sample, mixing and wash. Test the absorbance while rotate. Step motor rotates the reaction tray
by synchronization belt. Locate the reaction tray by the sensor and steps of motor.
2.3 Reagent System
Reagent system is for reagent and sample loading and dispensing. It consists of reagent rack,
reagent arm, reagent probe and reagent syringe etc.
2.3.1 Reagent Rack and Sample Rack
Reagent Rack Sample rack
Figure 2-12 Reagent and sample rack
Reagent rack is for reagent loading. With refrigeration function ensure the validity of reagent.
There are total 50 reagent positions for iMagic series. Sample rack are for storing sample, QC
and calibrator. There are totally 22 sample positions for iMagic series. The position for sample,
QC and calibrator can be defined by user.
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2.3.2 Probe
Step motor Collide protect
Synchronization belt
Sample Probe
Figure 2-13 Sample Probe
Sample probe is for reagent and sample dispensing from sample and reagent rack to reaction
cuvette.
Sample probe driving including motion motor and synchronization belt. There are sensors for
horizontal and vertical home position. Locate the probe move horizontal and vertical by steps of
step motor. Steps of step motor adjustment can change the position of the vertical and horizontal.
Sample probe has the function of liquid level detect and vertical collide protection. Liquid
level detect ensure the probe suck reagent and sample under the level of liquid and decrease
carryover. Collide protect make sure that the probe not damage while collide.
2.3.3 Ram Pump
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Figure 2-14 Ram pump
Ram pump is mainly used for sucking and draining reagent and sample, and washing the probe
interior, and transferring the reagent and sample to the related reaction cuvettes.
Step motor drive piston of ram pump move up and down. While the valve is close, the piston down,
ram pump suck reagent and sample. Otherwise, the piston up, it dispense reagent and sample to
cuvettes. While valve open, pump water to wash liquid pipeline.
2.4 Mixing System
DC motor
Probe
Mixing gear Reagent rack
Belt
Figure 2-15 Mixing system
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Mixing system mix after dispense reagent and sample to make sure reaction thoroughly.
iMagic series are using the sampling probe for mixing, it will mix after adding sample and reagents.
Mixing system includes mixing motor, synchronization belt, probe, eccentric gear and mixing
gear. Vertical sensor locates probe vertical home position. Horizontal sensor locates probe home
horizontal position. Step motors drive probe vertical and horizontal motion by synchronization belt.
2.5 Wash System
Clean cell
Wash arm
Figure 2-16 Wash system
Wash system includes two parts, one is to wash the cuvettes after the reaction finish, and
other is to wash the probe interior and exterior.
Wash system consists of pump, valve, liquid pipeline, etc.
iMagic series wash arm is 6 step auto wash, the sequence from right to left: the first, second,
third and forth steps are used to suck the wash solutions and inject deion water; the fifth and sixth
steps are used to suck the clean wash water. According to the 6 steps wash to make sure the
reaction cuvettes are clean and prepare for next time use.
The probe wash include interior and exterior wash. The probe needs to wash after sucking the
reagent / sample and putting to the cuvettes in order to avoid of cross-contamination between
samples and items. The probe also need back to wash well to clean after mixing in order to avoid
of cross-contamination between samples and items.
2.6 Control System
Control system is used to control motion of the whole system and signal processing. It
consists of main board, interface board, liquid pipeline drive board, signal adapt board, etc.
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Main board transfer control instruction from operation software to drive signal and send to
subsystem. Meanwhile, accept feedback signal to operation software.
Interface board accepts drive signal from main board to subsystem and feedback status
signal to main board.
Liquid pipeline drive board accepts drive signal from main board to drive pump and valve.
Signal adapt board accepts analog signal from pre-amplification board and transfer to
digital signal, then transfer to main board. It accepts feedback signal from temperature sensor of
reaction tray and send to main board.
2.7 System Connection
The Analyzer and PC are connected by RS232 port. Connect the RS-232 serial port line to the
computer and the analyzer respectively, and fasten the screws manually. In order to make sure
the machine work normally, the Power supply should be grounding well. The voltage between zero
line and grounding line should be less than 5V. The analyzer and the PC should be connecting to
the same power supply, and the UPS should not be less than 1500w.
The printer connects to PC directly and controlled by PC. There is no direct connection
between the printer and analyzer.
PC
iMagic series
Printer
Figure 2-17 Connection Diagram
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Chapter 3 System Installation
3.1 Check before Installation
Before installation, please check package carefully. If the package is not in good conditaion,
please declare to local distributor.
While unpackaged, please check appearance of instrument and check packing list. If there is
damage or anything lack, please take evidence and declare promptly to local distributor or iCubio
service department.
3.2 Installation Requirement
3.2.1 Space Requirement
Wall
≥50cm
≤300cm
Analyzer PC
Figure 3-1 Space requirement
3.2.2 Environment Requirement
1）Only for indoor installation.

2）The gradient of table board or ground should be less than 1/200.
3）Bear weight of table board or ground should be more than 500 kg.
4）Draughty.
5）Dust free.
6）Keep out of the sun shine.
7）Keep away from hot source and wind source.
8）Non-corrosiveness gas.
9）No shaking of table board or ground.
10）No noise source or power supply disturbance.
11）Keep away from brush motor or electrical contact device which switch frequently.
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12）Keep away from device emit electromagnetic wave such as mobile phone.
13）Altitude is less than 3000m.
3.2.3 Power Supply Requirement
1） Power supply is wideband AC is 220v, 50Hz. Power voltage fluctuation is ±10%;

Power supply line is 3 cores, good ground lead, The specifications of fuse protector
is:F5L250V.
2）Power of UPS should be greater than 1500w, lest test broke for sudden power lost.
3）Distance between socket and analyzer should be less than 2.5m.
Note.
1. Make sure ground lead well, otherwise its possible damage analyzer.
2. Make sure output of socket fit to requirement of system and with correct fuse.
3.2.4 Temperature and Humidity Requirement
1）Work temperature is between 15℃ to 30℃, fluctuation is less than ±2℃/H.

2）Relatively humidity is between 35%RH to 80%RH, no coagulation.
Note.
Out of the requirement, the system can’t make sure the reliability of data. If temperature and
humidity is out of requirement, please adjust is with air condition.
3.2.5 Water Supply and Dewatering Requirement
1） Quality of water must meet CAP secondary water requirement.

2） Temperature of water should between 15℃ to 32℃.
Organisms Infect Risk.

Please deal with waste liquid according to location discharge standard.
Note.
Quality of water must meet CAP secondary water requirement, otherwise test result maybe
disturbed by water.
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3.3 Installation
3.3.1 Water Bucket Connect
1）Connect wash water bucket. Connect two pipes to interface of analyzer, put another end of pipe
to bottom of bucket.
！！！Note: Please put something heavy at the end of pipe lest the pipe titling from bucket
bottom.
2）Connect waste bucket. Connect two waste liquid pipes to interface of analyzer, put another of
the pipes into waste bucket.
！！！Note: The waste pipes not dip into waste liquid while it is at high level, or else will
caused water overflow while washing needle.
3）Connect Liquid Level Sensor. Connect water and waste liquid level sensor to interface of
analyzer.
3.3.2 Connect RS232 Serial Cable and Power Line
1）Connect RS232 serial cable of accessory package to analyzer and PC.

2）Connect power line of accessory package to analyzer and socket correctly.
！！！Note. Please make sure the switcher of analyzer is off before connection of power line.
3）Switch on the switcher of analyzer, its power on.
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Chapter 4 Software
4.1 Software Installation
4.1.1 iChemMini Installation
4.1.1.1 iChemMini Working Environment
Operation system, Windows XP SP3，Windows 7，Windows 8.

Data base, Microsoft SQL Server 2008 Express Edition.
Other runtime:Microsoft . Net Framework above 4.0;
Language: Chinese/English
Hard disk: 200G；
Memory: 512M;
4.1.1.2 Content of Disc
Open the disc, content show as following.
Figure 4-1 Content of Disc
Ø AutoRun.exe: for opening the installation process. Customer can choose either typical
setup or Custom setup.
Ø Tools: Maintenance tools, only for customer service engineer use.
Ø Setup: Storage path of installation package. Users need not get into this document and
start installation.
Ø Install and Uninstall Guidance A1.0.Doc: Chinese set up and unload guidance.
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Ø Instruction for Installation and Uninstallation A1.0.doc: English set up and unload
guidance.
4.1.1.3 Installation Steps
Software installation is as following steps:

1） Backup data base before install;
2） Start installation program, choose typical setup. According to the needs, we will install
the following components:
a) Install Windows Installer 4.5
Silent install, installation interface did not display, walk-away installation.
b) Install Microsoft .Net Framework 4.0
Silent install, installation interface did not display, walk-away installation. Installation
time is around 10min.
c) Install Microsoft SQL Server 2008 Express Edition
d) Silent install, no installation interface, walk-away installation. Installation time is
around 15min.
e) Install iChemManager.
Default Installation, need to choose language and analyzer mode.
3） Start software to set parameters.
It’s necessary to check configuration of serial port after installation. Open “ChemSoft.ini” in the
fold of installation to find “SerialDeviceSetting=X,38400,0,8,0”, modify X to serial port No. of PC.
Please make sure whether the Baud rate is 38400, if it is not, it should be change to it manually.
Figure 4-2 Set serial port No.
How to check the serial port No: Click “my computer”à “property”à “hardware”à “device
manager”à “serial port” (checking from COM and LPT)
Make sure that the port No. is the same with setting of iChemManager.
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4.2 Installation Detail
4.2.1 Backup Data Before upgrade
Upgrading iChemmanager: software will automatically upgrade and it will compatible with
existed data. Make sure of the safety of database, please backup data before upgrade as
following.
1） Stop service of SQLServer2005.

Click “my computer” à “management”à “service and application program”à “SQL Server
Configuration Manager” à “SQL Server 2005 Services”à “SQL Server (ICUBIO)”à click stop.
2） Backup all data in the fold of install path.

（1） Find install path, default install path is “C:\Program Files\iChemMini”. It can
be found by click shortcut of iChemMini.
（2） Backup the whole fold.

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4.2.2 Start Install Program
Run “AutoRun.exe” on disk to start installation Program. Detailed installation steps, please
refer to documents of disk 《Instruction for Installation and Uninstallation A1.0.doc》
4.2.3 Start Software to Set Parameters.
1） Connect PC and analyzer with RS232 cable. Setting serial port No., default is COM1.
2） Power on.
3） Double click shortcut of “iChemMini” to start software. Select self-check while initialize.
Check whether initialization is finished or not.
4.3 Maintenance
Including daily maintenance, device check and absorbance test.
4.3.1 Daily Maintenance
Figure4-8 Daily maintenance
Aspirating and injection could be done and stop whenever in maintenance interface. Reading
background can get absorbance of 48 cuvettes. Save background to save it for zero calibration.
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4.3.2 Device Check
Figure4-9 Device check
All positions and status of machine can be check in “device check“ interface. It also can
be done to adjust position and modify parameters of device. Optic calibrations adjust the
position of light path.
4.3.3 Absorbance Test
Absorbance test check absorbance of 48 cuvettes to know about their status.
Figure 4-10 Absorbance Test

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Chapter 5 System Maintenance
5.1 Daily maintenance
1）Check water bucket to make sure enough water for test.

2）Empty waste bucket.
3）Check motion of X, Y, Z axis.
4）Check probe veritical and horizontal position at wash cell, reaction tray, reagent rack, sample
rack. Make sure probe does not clot.
5）Check wash weel does not overflow.
6）Check lamp and AD make sure they are normal.
7) Make sure the working tablet and surface of machine clean enough for operate.
5.2 Weekly Maintenance
1）Clean probe with detergent.

2）Test AD while take away cuvettes and save it.
3）Check cuvettes by absorbance test.
4）Clean wash bucket.
5）Clean waste bucket.
6）Clean reaction .
7）Clean sample rack.
8）Cuvettes disinfect.
9）Clean all surfaces of device by gauze with purified water.
10) Clean drainage needle and needle tip by gauze with absolute ethyl alcohol.
5.3 Monthly Maintenance
1）Check whether there are bubbles in ram pump.

2）Chemck lamp.
3）Check tightness of screw.
4）Check wash pipes to make sure they are cleanly and no blocking.
5）Clean x and y axis of sample arm.
6）Clean wash pipes.
7）Clean water filter and liquid level sensor.
8）Clean temperature control slot of reaction tray.
9) Clean anti-dust net.
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5.4 Half Year Maintenance
1）Check lamp.
2）Check ram pump.
3）Check pumps.
4）Check electromagnetic valves.
5）Check cuvettes.
6）Replace water pipes and waste pipes.
7）Replace water filters.
Chapter 6 Service
6.1 Reagent and Sample System Failure
Reagent and sample system is for loading reagent and sample. Reagent system with
refrigeration function ensure reagent stable.
6.1.1 Reagent Rack
There are two big racks and one small rack for loading reagent. Big rack and small rack can
be disassembled and exchanged freely according to use.
Figure 6-1 Reagent Rack
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6.1.2 Sample Rack
Figure 6-2 Sample Rack

There are two racks with 20 positions for normal sample and one rack with 2 positions for stat
test. The sample rack is fixed on the large base plate through the socket head screws. Sample
rack can be taken out independently.
6.1.3 Refrigeration Assembly
Refrigeration assembly is composed of cooling fin, peltier, peltier fixed seat and cooling fan.
Peltier is for reagent rack refrigeration to meet the temperature requirement for reagent freezing
and ensure reagent is always stored in low temperature environment to maintain stability.
Work principle of peltier is that one end emits heat and other end absorbs heat while current
pass through thermocouple. When peltier is power on, the heat of cold end is transferred to hot
end, so the cold end get colder and hot end get hotter.
Cooling fan
Peltier
Cooling fin
Figure 6-3 Refrigeration Assembly
Refrigeration assembly is composed of at least 1 Peltier, 1 cooling fan and 1 cooling fin to ensure
the refrigeration effect.
23 / 120
6.1.4 Reagent System Fault Point
Cooling Fan: While cooling fan is failed, peltier would not work, it means no refrigeration function
for reagent rack. Firstly check whether fan is working or not. If not, check the input 12V DC voltage
of fan is ok or not, if 12v is ok, it’s sure that fun is failed.
Peltier: While peltier is failed, there is no refrigeration function for reagent rack or with bad
refrigeration effect. If input 12V DC voltage is ok, it can be sure that peltier is failed. It should be
replaced.
Hall element sensor: It is used to confirm home position of reagent rack. While hall element is
failed, system will not detect the reagent rack home position. If input 3.3V DC voltage is ok, and
the magnet on the reagent rack is normal, then the hall element is failed and need to be replaced.
6.1.5 Sample System Failure
Hall element is used to confirm home position of reagent rack. While hall element is failed, system
will not detect the reagent rack. If input 3.3v DC voltage is ok, and the magnet on the reagent rack
is normal, then the hall element is failed and need to be replaced.
6.2 Probe System Failure
6.2.1 Probe Drive Assembly
Probe drive assembly is composed of linear bearing, lead screw, guide rail, motors of XYZ axis,
synchronization belt, belt gear, mixing motor and sample probe etc., the structure is as below:.
24 / 120
Figure 6-4 Probe Assembly
6.2.2 Probe Drive principle
Sample probe drive assembly is used to support sample probe, drive probe to move at x, y, z
dimension and mix to realize the probe movement between different position.
Z axis motor
Y axis motor
Mix motor
X axis motor
Y axis syn belt
X axis guide Y axis guid rail
X axis syn belt

Sample probe
Figure 6-5 Probe Drive Assembly
While x lead screw rotate, it drives probe move along x axis. Y synchronization belt drive by y axis
motor make probe move along y axis. Z motor drive z synchronization belt make probe up and
down according to drive instruction. Mixing motor drive eccentric gear make probe mixing.
25 / 120
Belt Liquid level detect
board
Drive gear
Collide protect
Adjusting screw
Eccentric gear
nut
Z synchronization belt
Z guide rail
Y home position sensor

shut
Little idler
Figure 6-6 Probe Z drive system
Collide protect sensor

Liquid level detect
board
Collide protect sensor
Clamp ring shut
Z up home position
Mixing motor sensor
Sensor shut
Eccentric gear
Lead rail slider
Probe
Collide protect
Figure 6-7 Collide Protect
While replace and maintain probe, firstly take away Z axis cover. Then do as following step, loose
collid protect sensor shut, pull out sample pipe, back-out clamp ring, take out hold-down spring,
take out probe from down to up. While install probe, please do according to reverse steps as
26 / 120
above. While probe installed, it’s necessary to check and adjust probe position by software. It can
be done in maintenance to select adjust.
6.2.3 Probe System Fault Point
Motor: While initialization or testing, the x, y or z axis doesn’t move and present that can’t detect
home position, if 24v DC voltage of motor is ok, step motor is fault.
Optocoupler: While initialization or testing, XYZ axis movement can’t detect home position,
please make sure that optocoupler shut of XYZ axis direction can move to correct position to shut
the sensor. If there is interference and collision, then check the 5v DC voltage for sensor to check
if it is correct. If OK, then the optocoupler is failed. The last point is sensor. While sensor for
collide protect is failed, the collide signal can’t send to main board, so system will give incorrect
drive signal lead to probe collide and damage it.
Liquid Level detect board: While liquid level detect board is failed, it can’t detect correct liquid
level signal and give wrong signal to main board. If probe is normal, but the liquid level detect is
not correct, the problem is liquid level detect board.
Sample probe: The probe and liquid level detect board finish liquid level detect function together.
While liquid level detect board is normal, the liquid level signal is fixed, the fault point is probe.
Collide protect assembly: While collide protect assembly has problem, probe can’t back to
correct position, the sensor shut is not in the sensor. So collide signal last while analyzer is power
on, analyzer will not work. Please check the probe’s position, the collide protect sensor and shut.
Little idler : it is used to fix synchronization belt. While it is loose, the synchronization belt will
loose and lead probe vertical motion failure. If it broke, synchronization belt can’t transfer motion
and lead probe vertical motion failure.
Liquid level detect line: It transfers the liquid level signal to main board, while it broke, the signal
transfer wrongly, main board will get wrong liquid level information.
Synchronization belt: It is used to transfer motion, while it is loose or broke, the motion
transmission will interrupt and lead probe move incorrectly and damage it. First check whether the
synchronous belt press block is loose or not compressed, and then check whether the driving
wheel or idler is loose, and then check whether the synchronous belt is fractured, if fractured and
need to change in time.
Mixing assembly: While mixing assembly got fault, it can’t mix reagent and sample normally. First
check whether the stirring belt is loose, and then check whether mixing motor wire welding, and to
check whether the motor power supply is normal (12 v), if the above checkpoint are normal, then
motor fault can be identified .
27 / 120
6.3 Filter System Failure
6.3.1 Filter System
Filter wheel system is composed of filter wheel motor, synchronization belt, belt wheel, gear, lamp,
lens, optocoupler etc.
Lamp→Lens→Filter→Cuvettes→Photoelectricity accepter→Signal adjust→Main board
6.3.2 Filter System Drive Principle
Filter system get monochromatic light from compound light of halogen lamp for testing. There are
8 filters in different wave length in the filter wheel. The wave length are 340nm, 405nm, 450nm,
510nm, 546nm, 578nm, 630nm, 700nm.
Convex lens
Halogen lamp
Lamp holder
Figure 6-9 Light system
6.3.3 Filter wheel system fault point
Halogen lamp: it emit compound light for testing, the light strength should be enough. While its
time is over one year or broken, it should be replaced. Open the lamp cover and loosen the fix
28 / 120
screws, the lamp assembly can be taken out freely. Replace new lamp assembly and fasten fix
screws, recover the lamp cover.
Note that halogen lamp is very hot, before replacing, should power off and wait for more than 30
minutes lest scald.
Optical filter wheel: There are 8 filters in the filter wheel, it rotates drive by step motor. There is one
sensor for home position of filter wheel, and position by steps of step motor. While filter wheel
rotate wrong, test result will be wrong. There are synchronization belt, gears, sensor, step motor
and input voltage of step motor should be check while there is failure of filter system.
6.4 Reaction Tray System
Reaction tray loads cuvettes and rotates them to designated location. It cooperate with probe,
wash needs to finish dispense reagent, sample to cuvettes and mix.
There is resistance wire heats reaction tray and temperature sensor to control the temperature in
the cuvettes is constant 37 ℃.
Figure 6-11 Reaction Tray
Reaction tray including of reaction drive assembly and reaction tray heat preservation assembly.
6.4.1 Reaction Tray Drive Assembly
It’s composed of reaction tray and reaction tray driving assembly. It loads cuvettes and rotate them
drive by step motor. While cuvettes get to pointed position for dispense reagent and sample,
mixing, wash, it will stop for action. While cuvettes pass photoelectricity detects position,
photoelectricity signal is detected.
Reaction tray drives assembly including of code disc assembly, motor assembly, and drive axis
assembly.
29 / 120
Code disc assembly is used for home position and tray rotate steps count. It is composed of rotate
code disc, sensor and sensor support.
Sensor
Sensor support
Rotate code disc
Figure 6-12 Code disc assembly
Drive motor assembly drives synchronization belt to rotate reaction tray. It records home position
while disc pass home position sensor, and then to count rotate steps.
Step motor
Synchronization
gear
Synchronization
belt
Engaged gear
Figure 6-13 Drive motor assembly
Drive axis is used for transfer rotation from step motor to reaction tray.
30 / 120
Axis
Bearing seat
Figure 6-14 Drive axis assembly
6.4.2 Heat Prevention Assembly
It is composed of reaction tray seat, heat belt, temperature sensor. It prevents heat diffuse fast to
ensure the temperature of reaction tray is stable.
Heat belt
Temperature
Reaction tray
seat
Figure 6-15 Heat Prevention Assembly
Reaction tray seat is made of metal, heat transfer fast and stable. There is orange heat belt to heat
reaction tray. Temperature sensor fixed in reaction tray to ensure the temperature is 37 ℃. While
it is lower than 37 ℃, heat belt work, while it higher than 37 ℃, heat belt stop work, so it is stale
for 37±0.1℃.
6.4.3 Reaction Tray System Fault Point
While step motor is broken, reaction tray will not rotate or rotate slowly. Firstly check whether
synchronization belt is loose. Then check input 24V DC voltage of step motor is ok.
31 / 120
Sensor is used for home position and rotates steps count, if sensor is failed, can’t detect home
position will be presented. To confirm sensor’s fault please check input 5v DC voltage of sensor is
normal.
Temperature sensor is for temperature control, while it is failed, reaction tray will not be heated or
always be heated, but temperature display is abnormal.
While heat belt work, temperature increase, otherwise it decrease. If heat belt is failed,
temperature of reaction tray can’t get to 37℃. To confirm it, firstly check whether input 24v DC
voltage of heat belt is normal. Then confirm the resistance of heat belt is between 16Ω and 18Ω.
6.5 Photoelectricity Detect System
Photoelectricity detect system is main part of chemistry analyzer. Its property decides the
precision and veracity of test result.
6.5.1 Detect Principle
Light source direct sunlight, convex lens can be converted into parallel rays, become bandwidth
monochromatic through interference filter, photodetector received monochromatic after absorbing
colorimetric by solution, photodetector converts intensity signal to electrical signals, FPU able to
calculate the concentration of solution by absorbing light range. Multi-Colorimetric wavelength of
Photometer system is realized by filter of filter wheel, the filter wheel turning that required
wavelength for achieve colorimetric measurement of each wavelength.
Lamp→Lens→Filter→Cuvettes→Photoelectricity accepter→Signal adjust→Main board
Signal adapt
board support
Signal adapt
board
Filter casing
Figure 6-17 Photoelectricity detect assembly
32 / 120
6.5.2 Matters Need Attention of Installation
While install filter, filter can’t be touch by finger or others hard thing, otherwise absorbance will be
affected. Jump ring should be fixed with tools such as screwdriver, there should be no scratch or
dirty of lens.
Place one paper in reaction groove, when the light focus at one point, it means that lens is in
correct position.
Filter wheel should be rotated lightly into axis and fixed with socket head cap screw.
While install signal adapt board, the photodiode should be buried in filter casing avoid stray light.
Lens hood should be installing after signal adapt board installation.
6.5.3 Photoelectricity Detect System Fault Points
Optical Filter: Generally characterized by a single wavelength absorbance on the high side or AD
value is low, may also be a small absorbance or AD value is very high, need to eliminate the
situation of light saturation.
Signal adapt board: it accept light signal and transfer to electrical signal then converts to digital
signal. While it is failed, the AD and absorbance will be abnormal, it need to analyze carefully.
Cuvettes problem: generally shows in the form of individual phenomenon, when photoelectric
acquisition position incline will cause high absorbance or low AD value for most of the
wavelengths, through adjusting light exact position to confirm the location problem of the cuvettes.
Halogen light: it emits enough strong compound light for test. While it broke or decayed, the light
strength decrease, AD for all filters will be low, especially for 340nm.
Data cable: when the data cable which connect AD board and photoelectric receiving plate loosen
or poor contact, can lead to abnormal AD values of individual wavelengths, in extreme cases, can
also lead to abnormal AD for all the wavelengths.
6.6 Liquid Flow Path
Liquid flow path including of pump, valve, ram pump and pipes. The liquid flow path of each
model is blow
33 / 120
Figure 6-18 Liquid Flow Path ( with auto cleaning)
34 / 120
Figure 6-19 Liquid Flow Path (with detergent )
35 / 120
Figure 6-20 Liquid Flow Path (without auto cleaning)
6.6.1 Valve
Breaker
press valve Solenoid
valve
Figure 6-21 Valves
36 / 120
Solenoid valve is used to control liquid pass or not to probe. If there is dirty or some other things
in the valve, the valve can’t open or close correctly lead to probe dropping, reagent and sample
dispense failure and so on. Breaker press valve is used to control back suck while dispense
clean water to cuvettes. While it broke, cuvettes will outflow while dispense clean water. Both
valve input voltage is DC 12v.
6.6.2 Disc Pump
Figure 6-22 Disc Pump

Application Module: Pump 1 and pump 2 are responsible for water supplying for washing arm, ram
pump and washing cup. Pump 3 and pump 4 are responsible for aspirating water of washing arm.
Function: when disc pump is on power, cleaning water can flow to washing cuvettes based on
pressure and speed from the pump entrance and exit. Therefore, the needle and stirrer can be
washed. The pure water can flow to wash arm, wash cuvettes, injector and so on. The power is
both pump driver is 24V.
While pump broken, the probe, stirrer unable be washed, which influence test result . If pump
which providing water for stirrer washing is broken, there will be no water for cuvettes wash. It will
result in cross-contamination and influence test result. If drainage pump failed, the water will
overflow.
.
6.6.3 Ram Pump
Ram pump dispense reagent and sample accurately together with valve.
37 / 120
Figure 6-23 Ram Pump
Application components: the structure of the plunger pump: by micro step by step motor, pump
body, pump head, ceramic plunger, fixed frame, optical coupling, etc.
Function:
1, Sample taking and adding:

When the ram pump take the sample, valve connecting the water pump will stop supply water. The
ram pump piston will be pulled down by driver assembly and the piston be vacuum, which create
negative pressure for internal liquid path. The sampler absorbs the sample. Its precision is
controlled by step motor. When adding sample, it make returning-fill in piston, which bring positive
pressure for liquid path. It can drain out the absorbed liquid, finishing the sampling process.
2, Inner wall washing of sample probe
While washing the sample probe inner wall, plunger pump piston moves up and down,
electromagnetic valve connected pump open water, water flow from electromagnetic valve to
sample probe, to rinsing the sample probe inner wall, finish washing action with washing cups
washing to reduce the pollution of the cross and carry.
Plunger pump drive: plunger pump piston is fixed by the piston seat, and then fixed on the
screw nut of stepping motor, the step motor work makes the screw rotation. So by motor work can
realize the up and down movement of plunger piston pump, piston internal filling and empty,
38 / 120
Through light coupling and coupling shut to record the initial position of the piston, with the motor
steps control, to realize the precision injection of plunger pump.
6.7 Wash Arm System Fault Point
Washing arm system is used for washing reaction cup by the action of sucking the waste liquid
from the reaction cup -inject into the reaction cup distilled water – suck detergent from reaction cup.
iMagic series can realize 6 steps auto washing. The first, second, third, fourth steps are for
absorbing the detergent from the reaction cup and inject into deionized water inside the reaction
cup, the fifth step is for sucking the clean water from the reaction cup, the sixth step is for wiping
siphon off the rest of the water in the cup, through 6 steps to ensure the reaction cup clean after
cleaning for the next cycle use.
Wash probe
Probe fix socket
Drier tip
Figure 6-24 Wash Arm
6.7.1 Wash Arm Drive Principle
Wash arm system is composed of step motor, synchronization belt, hall element, U type
bearing, support, wash needle and needle seat. Wash needs go down and up drived by step
motor.
39 / 120
Wash needle
Needle seat
assembly
Support
Hall element
U type bearing
Step motor
Synchronization belt
Figure 6-25 Wash Arm
6.7.2 Wash Arm System Fault Point
While DC 24v for step motor is correct, but the wash arm does not move and present that it can’t
detect home position, it can be sure that step motor is broken.
While wash arm move to home position but at present it can’t detect home position and step motor
sound abnormal, it should be hall element problem or magnet fixed in the arm is failed.
Little idler is used to tight synchronization belt, while it is loose, synchronization belt can’t transfer
rotation of step motor to vertical motion of wash arm correctly.
Synchronization belt transfer rotation of step motor to vertical motion of wash arm, if it is loose or
broken, wash arm will move incorrectly.
Clean needles are used to aspirate or suck liquid from cuvettes, while needles fixed incorrectly, it
will lead to collide or aspirate abnormal. Or needles is clot, the needle can’t aspirate water into
cuvettes or can suck waste liquid from cuvettes. Cuvettes will outflow.
6.8 PCBA Replace
M Serial PCBA control flow show as following diagram.
40 / 120
Note：R.F.W.P Board means Reaction tray, Filter rotator, Wash arm, Pump interface board.
41 / 120
PCBA interface：
6.8.1 Main Board
1. Power Interface (J17)

J17 +5V GND
1
2
3 +12V
4
5 -12V
C0N5
Pin Function
1 +5V
2 Digital Ground
3 +12V
4 Analog Ground
5 -12V
2. Reagent, Sample Rack, Panel LED Interface (J12)
J12
GND 1 2 GND
RUN_LED1 R_P_LED1
3 4
P_COVER1 P_COVER2
5 6
M_COVER1 S_RACK1
7 8
S_RACK2 SJ_R1
9 10
VCC_3.3V 11 12 VCC_3.3V
SJ_R2 SJ_R3
13 14
SJ_R5 SJ_R4
15 16
EM_LAMP EMG_R
17 18
GND 19 20 GND
Header 10X2
Pin Function
1,2,19,20 Digital Ground
11,12 +3.3V
3 RUN_LED1
4 R_P_LED1
5 P_COVER1
6 P_COVER2
7 M_COVER1
8 S_RACK1
9 S_RACK2
10 SJ_R1
13 SJ_R2
14 SJ_R3
15 SJ_R5
16 SJ_R4
17 EM_LAMP
42 / 120
18 EMG_R
3. Photoelectricity Signal Sampling Interface (J13)
GND
C156
J13 0.1uF
1 2
C157 0.1uF AD_MOSI AD_SPCLK
GND 3 4
GND 5 6 GND
AD_CS AD_MISO C155 0.1uF
7 8 GND
S_SDI SPI_REQ
9 10
C158 0.1uF +9V -9V
GND 11 12
S_CS1 S_SDO C154 0.1uF
13 14
S_SCLK S_CS0
15 16
C153 17 18
GND
19 20
0.1uF
+5V +5V
Header 10X2
GND
Pin Function
1,2,17,18 Analog Ground
19,20 +5V
5,6 Digital Ground
11 +9V
12 -9V
3 AD_MOSI
4 AD_SPCLK
7 AD_CS
8 AD_MISO
9 S_SDI
13 S_CS1
14 S_SDO
15 S_SCLK
16 S_CS0
4. Liquid, Reaction Tray, Wash Arm & Ram Pump (J10)
43 / 120
J10
GND 1 2 GND
C161 220pF W_STEP W_EN
GND 3 4
W_DIR W_LP
5 6
VAVLE51 VAVLE41
7 8
VAVLE31 VAVLE21
9 10
VAVLE11 PUMP51
11 12
C163 220pF PUMP61
GND 13 14 GND
PUMP41 PUMP31
15 16
PUMP21 PUMP11
C170 220pF 17 18
GND CODE_CLK CODE_CS
19 20
CODE_DO MSDI C165 220pF
21 22
C159 220pF MSCLK MCS1
GND 23 24
FYP_EN1 FYP_DIR1
25 26
C160 220pF FYP_STEP1 CODE_PDIO C167 220pF
GND 27 28
FYB_POS MSDO
29 30 GND
C166 220pF 1WIRE3 FYP_0 C168 220pF
GND 31 32
1WIRE1 1WIRE2
33 34
H_W_CON R1_H
35 36
C164 220pF LAMP_LP1
GND GND 37 38
LAMP_SW1 R1_VAL C169 220pF
39 40
PT_CON R1M_LP
41 42
R1M_STEP R1M_EN
43 44
R1M_DIR R1JECT_HOM
C162 45 46
W_V_HOM W_COLLIDE
47 48
220pF
49 50 GND
Header 25X2
GND
Pin Function
1,2,14,37,49,50 Analog Ground
3,4,5,6 Wash Arm Motor Driving
7,8,9,10,11 Valve Signal1-5
12,13,15,16,17,18 Pump Signal 1-6
19,20,21,28 Bar Code Signal
22,23,24,30 SPI Read & Write of TMC262
25,26,27 Reaction Tray Motor Driving
29 Reaction Tray
Position(FYP_POS)
32 Reaction Tray Home
Position(FYP_0)
31 1WIRE1-Reaction Temperature
35 H_W_CON-Reaction Tray
Temperature Control
36 R1_H-Reagent 1 Heat Control
38 LAMP_LP1-Lamp Low Power
39 LAMP_SW1-Lamp Switch
40 R1_VAL-Reagent Valve
41 PT_CON-Reaction Tray
Temperature Control
46,44,45,46 Ram Pump Motor Control
47 W_V_HOM-Wash Arm Vertical
Sensor
48 W_COLLDE-Wash Arm Collide
44 / 120
Protect
5. XYZ & Filter Motor Signal (J11)
J11
GND 1 2 GND
C171 Y_EN1 Y_DIR1
3 4
C173 Y_STEP1 X_EN1
GND 5 6
X_STEP1 X_DIR1
7 8
220p X_CS Y_COUNT
9 10
220p Y_END1 Y_0
11 12
GND X_0 X_COUNT
13 14
GND 15 16 GND
X_CAL2 X_CAL1
17 18
X_END R2_CO
19 20
R1_CO Z_LP1
21 22
C172 Z_EN1 Z_DIR1
23 24
Z_STEP1 Y_LP1
GND 25 26
MIX_M LEVEL_TEST
27 28
220p Z_COLLIDE
29 30 GND
Z_CAL Z_COUNT
31 32
Z_0
GND 33 34
Y_CAL2 Y_CAL1
35 36
Q_LEVEL WA_LEVEL
37 38
W_LEVEL F_POS
39 40
F_HOM Q_CCW-
41 42
Q_CCW+ Q_CW-
43 44
Q_CW+ F_LP
45 46
C174 F_EN F_DIR
47 48
F_STEP
GND 49 50 GND
220p Header 25X2
Pin Function
1,2,15,16,30,33,50 Analog Ground

3,4,5 Y Axis Motor Driving
6,7,8,9 X Axis Motor Driving
10 Y_COUNT-Y Axis Motion Count
Signal
11 Y_END1-Y Axis End Position
12 Y_0-Y Axis Home Position
13 X_0-X Axis Home Position
14 X_COUNT-X Axis Count Signal
17 X_CAL2-Y Axis Motion Count
Signal
Signal
19 X_END-X Axis End Position
20 R2_CO-Reagent 2 Refrigerate
22,23,24,25 Z Axis Motor Driving
45 / 120
26 Y_LP1-Y Axis Motor Low Power
27 MIX_M-Mixing Motor
28 LEVEL_TEST-Liquid Level Detect
Signal
29 Z_COLLIDE-Z Axis Collide
Protect
31 Z_CAL-Z Axis Motor Count
32 Z_COUNT-Z Axis Motor Motion
Count
34 Z_0-Z Axis Motor Home Position
35 Y_CAL2-Y Axis Motor Count 2
37 Q_LEVEL-Detergent Level
38 WA_LEVEL-High Concentration
Waste Liquid Level
39 W_LEVEL-Waste Liquid Level
40 F_POS-Filter Rotator Motor
Position
41 F_HOM-Filter Rotator Motor
Home Position
42 Q_CCW--Wash Liquid Motor
Driving
43 Q_CCW+- Wash Liquid Motor
Driving
44 Q_CW--Wash Liquid Motor
Driving
45 Q_CW+- Wash Liquid Motor
Driving
46,47,48,49 Filter Rotator Driving Signal
6.8.2 Motor Driving Board
1. Liquid, Reaction Tray, Wash Arm & Ram Pump Motor Signal (J1)
46 / 120
J1
GND 1 2 GND
QXA_V_STEP QXA_V_EN
3 4
QXA_V_DIR QXA_V_LP
5 6
VAVLE51 VAVLE41
7 8
VAVLE31 VAVLE21
9 10
VAVLE11 PUMP51
11 12
PUMP61
13 14 GND
PUMP41 PUMP31
15 16
PUMP21 PUMP11
17 18
CODE_CLK CODE_CS
19 20
CODE_DO MSDI
21 22
MSCLK MCS1
23 24
FYP_EN1 FYP_DIR1
25 26
FYP_STEP1 CODE_PDIO
27 28
FYP_POS MSDO
29 30
1WIRE3 FYP_0
31 32
1WIRE1 1WIRE2
33 34
H_W_CON R1_H
35 36
LAMP_LP1
GND 37 38
LAMP_SW1 R1_VAL
39 40
PT_CON R1M_LP
41 42
R1M_STEP R1M_EN
43 44
R1M_DIR R1JECT_HOM
45 46
QXA_V_HOM QXA_V_COLLIDE
47 48
GND 49 50 GND
Header 25X2
Pin Function
1,2,14,37,49,50 Analogy
3,4,5,6 Wash Arm Motor Driving
7,8,9,10,11 Valve Single 1-5
12,13,15,16,17,18 Pump Signal 1-6
19,20,21,28 Bar Code Signal
22,23,24,30 SPI Read & Write of TMC262
25,26,27 Reaction Tray Motor Driving
29 Reaction Tray
Position(FYP_POS)
32 Reaction Tray Home
Position(FYP_0)
35 H_W_CON-Reaction Tray
Temperature Control
36 R1_H-Reagent 1 Heat Control
38 LAMP_LP1-Lamp Low Power
39 LAMP_SW1-Lamp Switch
40 R1_VAL-Reagent Valve
41 PT_CON-Reaction Tray
Temperature Control
46,44,45,46 Ram Pump Motor Control
47 / 120
47 W_V_HOM-Wash Arm Vertical
Sensor
48 W_COLLDE-Wash Arm Collide
Protect
2. XYZ Axis & Filter Rotator Motor Signal (J2)
J2
GND 1 2 GND
Y_EN1 Y_DIR1
3 4
Y_STEP1 X_EN1
5 6
X_STEP1 X_DIR1
7 8
X_CS Y_COUNT
9 10
Y_END1 Y_0
11 12
X_0 X_COUNT
13 14
GND 15 16 GND
CODE_CS2 X_CAL1
17 18
X_END R2_CO
19 20
R1_CO Z_LP
21 22
Z_EN Z_DIR
23 24
Z_STEP Y_LP1
25 26
MIX_M LEVEL_TEST
27 28
Z_COLLIDE
29 30 GND
Z_CAL Z_COUNT
31 32
Z_0
GND 33 34
CODE_CS1 Y_CAL1
35 36
Q_LEVEL WA_LEVEL
37 38
W_LEVEL F_POS
39 40
F_HOM Q_CCW-
41 42
Q_CCW+ Q_CW-
43 44
Q_CW+ F_LP1
45 46
F_EN1 F_DIR1
47 48
F_STEP1
49 50 GND
Header 25X2
Pin Functionu
1,2,15,16,30,33,50 Analog Ground
3,4,5 Y Axis Motor Driving
6,7,8,9 X Axis Motor Driving
10 Y_COUNT-Y Axis Motion Count
Signal
11 Y_END1-Y Axis End Position
14 X_COUNT-X Axis Count Signal
Signal
Signal
22,23,24,25 Z Axis Motor Driving
48 / 120
26 Y_LP1-Y Axis Motor Low Power
27 MIX_M-Mixing Motor
Signal
29 Z_COLLIDE-Z Axis Collide
Protect
31 Z_CAL-Z Axis Motor Count
Count
37 Q_LEVEL-Detergent Level
38 WA_LEVEL-High Concentration
Waste Liquid Level
39 W_LEVEL-Waste Liquid Level
40 F_POS-Filter Rotator Motor
Position
41 F_HOM-Filter Rotator Motor
Home Position
42 Q_CCW--Wash Liquid Motor
Driving
43 Q_CCW+- Wash Liquid Motor
Driving
44 Q_CW--Wash Liquid Motor
Driving
45 Q_CW+- Wash Liquid Motor
Driving
46,47,48,49 Filter Rotator Driving Signal
3. Ram Pump & Wash Arm Motor signal (J3)
49 / 120
+12V
J3
R1M_A1 R1M_A2 D11
1 2 5817 C70
R1M_B1 R1M_B2
3 4
R1_VALO
5 6
GND 7 8 +12V
0.1uF
9 10
C89 0.1uF QXA_V_COLLIDE QXA_V_HOM
11 12
R1JECT_HOM
GND 13 14
15 16 VCC_3.3V
QXA_V_B2 QXA_V_A1 C91 C92
17 18
QXA_V_A2 QXA_V_B1 0.1uF 0.1uF
19 20
Pin Function
5,7,9,13,15 Analog Ground
8 +12V
16 +3.3V
1,2,3,4 Ram Pump Motor A & B Phase
Winding
6 R1_VALO-Ram Pump Valve Control
11 QXA_V_COLLIDE-Wash Arm Collide
Protect
12 QXA_V_HOM-Wash Arm Position
14 R1JECT_HOM-Ram Pump Motor
Home Position
17,18,19,20 Wash Arm Motor A & B Phase
Winding
4. Reaction Tray, Filter Rotator Motor Signal (J5)
50 / 120
J5
FYPB2
VCC_3.3V 1 2
FYPB1
3 4
FYPA2
5 6
FYPA1
7 8
9 10
11 12
C93 FYP_0 CODE_CS
13 14
FYP_POS CODE_CLK
15 16
CODE_PDIO CODE_DO
17 18
0.1uF VCC_3.3V
19 20
F_A1
21 22
F_B1 F_HOM C88 0.1uF
23 24
F_A2 F_B2
25 26
F_POS C87 0.1uF
27 28
Header 14X2
Pin Function
10,11,12,19,20,27 Analog Ground
1,3,22 +3.3V
2,4,6,8 Reaction Tray Motor A & B Phase
Winding
11 FYP_0-Reaction Tray Home Position
15 FYP_POS-Reaction Tray Position
14,,16,17,18 Reaction Tray Motor Feedback
21,23,24,25 Filter Rotator Motor A & B Phase
Winding
24 F_HOM-Filter Rotator Home Position
28 F_POS-Filter Rotator Poistion
5. XYZ Axis Motor Signal (J6)
J6
C69 0.1uF MIX_MO
1 2 +12V
C81 C80 C79 C78 C77 C76 C75 C73 C72 C71 Y_B1
3 4
Y_A1
GND 5 6
0.1uF 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF CODE_CS1 Y_A2
7 8 C82 C83 C84 C85 C86
CODE_PDIO Y_B2
9 10
+24V 11 12 +24V 0.1uF 0.1uF 0.1uF 0.1uF 0.1uF
Z_0 R1_H
13 14
CODE_CLK CODE_DO
15 16
Z_COUNT X_COUNT
17 18
Y_0
GND 19 20
Z_COLLIDE Y_COUNT
21 22
LEVEL_TEST
23 24 VCC_3.3V
X_END
25 26
CODE_CS2
27 28 GND
X_0 X_B2
29 30
Y_END1 X_B1
31 32
X_A2
33 34
X_A1
GND 35 36
Z__B2 Z__B1
37 38
Z__A2 Z__A1
39 40
Pin Function
3,5,19,28,33,35 Analog Ground
24,26 +3.3V
2 +12V
11,12 +24V
1 Mixing Motor Signal
4,6,8,10 Y Axis Motor A & B Phase Winding
7,9,15,16 X Axis Motor Position FeedBack
14 R1_H-Reagent Pre-heat Control
51 / 120
18 X_COUNT—X Axis Motor Position
22 Y_COUNT-Y Axis Motor Position
21 Z_COLLIDE-Z Axis Collide Protect
25 X_END-X Axis Motor End Position
27 CODE_CS2-Motor Position
Feedback CS(Chip Selection) Signal
31 Y_END1-Y Axis Motor End Position
30,32,34,36 X Axis Motor A & B Phase Winding
37,38,39,40 Z Axis Motor A & B Phase Winding
6. Heat, Refrigerate, Pump Control Signal

J8
LAMP_SW1
1 2 GND
PT_CON LAMP_LP1
3 4
+5V 5 6 +5V
WA_LEVEL Q_LEVEL
7 8
W_LEVEL
9 10 +12V
11 12
GND 13 14 GND
15 16
GND R2_CO
17 18
R1_CO
19 20 +12V
PUMP21 PUMP11
21 22
PUMP41 PUMP31
23 24
25 26 GND
VAVLE21
GND 27 28
VAVLE31 VAVLE51
29 30
+24V 31 32 +24V
Header 16X2
Pin Function
2,11,12,13,14,15,16,17,25,26,27 Analog Ground
24,26 +3.3V
2 +12V
11,12 +24V
1 Mixing Motor
7,9,15,16 X Axis Motor Position Feedback
18 X_COUNT-X Axis Motor Position
52 / 120
Feedback CS Signal
7. Power Interface (J7)
C67 C68
470uF/50V 0.1uF
J7 +24V
1
1
2 +12V
2
3 C66 0.1uF
3
4
4
CON4
C65 470uF/25V
Pin Function
2,4 Analogy Ground
1 +24V
3 +12V
6.8.3 Signal Adapt Board
1. Signal Interface (J1)

J1
1 2
C23 0.1uF AD_MOSI AD_SPCLK C28 0.1uF
3 4
5 6
AD_CS AD_MISO C27 0.1uF
7 8
C24 0.1uF S_SDI SPI_REQ
9 10
+9V -9V
11 12
S_CS1 S_SDO C26 0.1uF
13 14
S_SCLK S_CS0
15 16
0.1uF C25
17 18
+5V 19 20 C30
C5
C29 +5V 470U/25V
Header 10X2
C32 470U/25V 0.1uF
0.1uF
C31
C33 470U/25V
0.1uF
Pin Function
1,2,17,18 Analogy Ground
19,20 +5V
5,6 Digital Ground
53 / 120
11 +9V
12 -9V
3 AD_MOSI
4 AD_SPCLK
7 AD_CS
8 AD_MISO
9 S_SDI
13 S_CS1
14 S_SDO
15 S_SCLK
16 S_CS0
2. Signal Accept Board Interface (J2)
J2
1
1 +9V
2
2
3 S
3
4
4 -9V
CON4
Pin Function
1 +9V
2 Analogy Ground
3 S(Photoelectricity Signal)
4 -9V
6.8.4 Signal Accept Board (J1)
J1
1
1 +9V
2
2
3 Signal
3
4
4 -9V
CON4
Pin Function
1 +9V
2 Analogy Ground
3 Signal(Photoelectricity Signal)
4 -9V
54 / 120
6.8.5 X Axis Motor Interface Board
1. XYZ Axis Motor Signal Interface (J1)

J1
MIX_MO
1 2 +12V
Y_B1
3 4
Y_A1
GND 5 6
CODE_CS1 Y_A2
7 8
CODE_PDIO Y_B2
9 10
+24V 11 12 +24V
Z_0 R1_H
13 14
CODE_CLK CODE_DO
15 16
Z_COUNT
17 18
Y_0
GND 19 20
Z_COLLIDE
21 22
LEVEL_TEST
23 24 VCC_3.3V
X_END
25 26
CODE_CS2
27 28 GND
X_0 X_B2
29 30
Y_END1 X_B1
31 32
X_A2
33 34
X_A1
GND 35 36
Z__B2 Z__B1
37 38
Z__A2 Z__A1
39 40
Pin Function
3,5,19,28,33,35 Analogy Ground
24,26 +3.3V
2 +12V
11,12 +24V
1 Mixing Motor Signal
22 Y_COUNT- Y Axis Motor Position
27 CODE_CS2-Motor Position CS
Signal
2. YZ Axis Motor Signal Interface (J3)
55 / 120
J3
MIX_MO
1 11 GND
Z_COUNT
+12V 2 12
CODE_DO
3 13
Y_END1
4 14 GND
CODE_CS2 CODE_CLK
5 15
R1_H
VCC_3.3V 6 16
7 17 GND
LEVEL_TEST Z_0
8 18
Z_COLLIDE CODE_PDIO
9 19
Y_0
10 20 GND
Pin Function
6,7 +3.3V
2,3 +12V
4 Y_END-Y Axis Motor End Position
1 MIX_MO-Mixing Moto
Count
5,13,15,19 Motor Position Feedback
16 R_H-Reagent Pre-heat
9 Z_COUNT-Z Axis Collide Protect
18 Z_0-Z Axis Home Position
3. Y Axis Motor Phase Winding Signal Interface (J6)
J6
Y_B2
1
Y_A2
2
Y_A1
3
Y_B1
4
Header 4
4. Z Axis Motor Phase Winding Signal Interface (J8)

J8
Z__B2
1
Z__A2
2
Z__A1
3
Z__B1
4
Header 4
5. X Axis Motor Position Feedback Signal Interface (J7)
56 / 120
CODE_CLK
R2 C1
4.7K 100pF
VCC_3.3V J7
6
CODE_CS1
5
GND 4
CODE_DO
3
C2 2
R3
100pF 1
4.7K
GND
GND
CODE_PDIO
R4 C3
4.7K 100pF
GND
6. X Axis Motor Photo Winding Signal Interface (J2)

J2
X_B2
1
X_B1
2
X_A2
3
X_A1
4
Header 4
7. X Axis End Position(X_01 Interface (J4)
J4
VCC_3.3V 1
X_END
2
GND 3
Header 3
8. X Axis Home Position (X_0) Interface (J5)

J5
VCC_3.3V 1
X_0
2
GND 3
Header 3
6.8.6 Y Axis Motor Interface
1. YZ Axis Motor Signal Interface(J1)

J1
MIX_MO
1 2 +12V
Y_END1
+12V 3 4
CODE_CS2
5 6 VCC_3.3V
LEVEL_TEST
VCC_3.3V 7 8
Z_COLLIDE Y_0
9 10
Z_COUNT
GND 11 12
CODE_DO
13 14 GND
CODE_CLK R1_H
15 16
Z_0
GND 17 18
CODE_PDIO
19 20 GND
Pin Function
57 / 120
6,7 +3.3V
2,3 +12V
4 Y_END-Y Axis End Position
1 MIX_MO-Mixing Motor
12 Z_COUNT-Z Axis Motor Count
5,13,15,19 Motor Position Feedback
2. Z Axis Motor Signal Interface (J3)
J3
Z__B2 Z__A2
1 2
Z__A1 Z__B1
3 4
LEVEL_TEST
VCC_3.3V 5 6
Z_COLLIDE Z_COUNT
7 8
R1_H
9 10
Z_0
11 12 +24V
+24V 13 14
15 16 GND
GND 17 18
MIX_MO
19 20 +12V
Header 10X2H
Pin Function
14,15,16,17,18 simulation
12,13 +24V
20 +12V
5 +3.3V
11 Z_O-Z Axis Home Position
3. Y Axis Motor Phase Winding Interface (J6)
J6
Y_B2
1
Y_A2
2
Y_A1
3
Y_B1
4
Header 4
4. Z Axis Motor Phase Winding Interface (J8)
58 / 120
J8
Z__B2
1
Z__A2
2
Z__A1
3
Z__B1
4
Header 4
5. X Axis Motor Position Feedback Interface (J7)
VCC_3.3V J7
6
CODE_CS2
5
CODE_CLK
4
CODE_DO
3
CODE_PDIO
2
1
GND
6. Y Axis Motor Phase Winding Interface (J2)
J2
Y_B2
1
Y_A2
2
Y_A1
3
Y_B1
4
Header 4
7. Y Axis End Position (Y_END1) Interface (J4)
J4
VCC_3.3V 1
Y_END1
2
GND 3
Header 3
8. Y Axis Home Position (Y_0) Interface (J5)
J5
VCC_3.3V 1
Y_0
2
GND 3
Header 3
6.8.7 Heat & Refrigerate Board
1. Heat & Refrigerate Board Interface
59 / 120
C11
GND
0.1uF
J7
LAMP_SW1
1 2
C14 0.1uF PT_CON LAMP_LP1 C15 0.1uF
3 4
+5V 5 6 +5V
WA_LEVEL Q_LEVEL
7 8
W_LEVEL
9 10 +12V
11 12
GND 13 14 GND
15 16
GND R2_CO
17 18
R1_CO
19 20 +12V
PUMP21 PUMP11
21 22
PUMP41 PUMP31
23 24
25 26 GND
VAVLE21
GND 27 28
VAVLE31 VAVLE51
29 30
+24V 31 32 +24V
Header 16X2
Pin Function
2,11,12,13,14,15,16,17,25,26,27 Analog Ground
24,26 +3.3V
2 +12V
11,12 +24V
1 Mixing Motor
Feedback CS Signal
2. Power Interface (J1 J5)
J1
1 J5 +24V
1 +12CV 1
2 1
2 2
2
+5CV
3 3
3 3
4 4
4
4 AGND 5
5
CON4 CON5
3. Valve Control (J10)

60 / 120
+24V
BAK2_V
J10
+24V
6
5
4
IN_SUCK
3
2 +24V
1
MHDR1X6
NEEDLE_O_WASH1
Pin Function
2,4,6 +24V
1 BAK2_V-Ram Pump Valve
3 IN_SUCK-Suck Valve
5 NEEDLE_O_WASH-Probe Outside
Wash Valve
4. Pump Control Interface (J11)
+24V
NEEDLE_IN
J11 +24V
8
7 INCUBER_IN
6
5
4
+24V
3
2
1
H_CON_OUT
MHDR1X8
+24V
DRY_OUT
Pin Function
2,4,6,8 +24V
1 DRY_OUT-Dry Waste Pump
3 H_CON_OUT-High Concentration Waste
Pump
5 INCUBER_IN-Cuvettes Water Pump
7 NEEDLE_IN-Probe Inside Wash Pump
5. Water, Waste Liquid Level Detect (J6)
J6 +5V
W_LEVEL R9 10K
1
2
WA_LEVEL R10 10K
3
4
Header 4
C12 C13
0.1uF 0.1uF
Pin Function
61 / 120
2,4 Ground
1 W_LEVEL-Water Liquid Level
3 WA_LEVEL-Waste Liquid Level
6. Wash Liquid Level Signal (J9)
J9 +5V
1
Q_LEVEL
2
3 C19
Header 3 0.1uF
7. Refrigerate Signal (J8)
+12CV
J8
1
R1_COOL
2
3
R2_COOL
4
Header 4
Pin Function
1,3 +12V
2 R1_COOL-Refrigerate Signal 1
4 R2_COOL-Refrigerate Signal 2
8. Heat Signal (J3)
+24V
J3
1
HEATOR
2
CON2
9. Lamp Control (J2)
62 / 120
J2
CON2
2
1
2
1
+5CV
LAMP_CON
6.8.8 Z Axis Interface Board
1. Main Signal Interface

J1
Z__B2 Z__A2
1 2
Z__A1 Z__B1
3 4
LEVEL_TEST
VCC_3.3V 5 6
Z_COLLIDE Z_COUNT
7 8
Z_CAL R1_H
9 10
Z_0
11 12 +24V
+24V 13 14
15 16 GND
GND 17 18
MIX_MO
19 20 +12V
Header 10X2H
Pin Function
14,15,16,17,18 Analog Ground
12,13 +24V
20 +12V
5 +3.3V
2. Liquid Level Detect Board Interface
+12V J3 +12V
1 2
MIX_MO MIX_MO
3 4
Z_COLLIDE
5 6 GND
LEVEL_TEST
GND 7 8
9 10
Header 5X2 VCC_3.3V
63 / 120
Pin Function
1,2 +12V
10 +3.3V
6,7,9 Ground
3,4 MIX_MO-Mixing Signal
3. Z AxisMotor Phase Winding Interface
J2
Z__B2
1
Z__A2
2
Z__A1
3
Z__B1
4
Header 4
4. Z Axis Position Interface

J4
VCC_3.3V 1
Z_0
2
GND 3
Header 3
5. Probe Pre-heat Interface

J5
GND 1
+24V 2
R1_H
3
Header 3
6.8.9 Liquid Level Detect Board
1. Main Signal Interface

+12V J1 +12V
1 2
MIX_MO MIX_MO
3 4
Z_COLLIDE
5 6 GND
LEVEL_TEST
GND 7 8
9 10
Header 5X2 VCC_3.3V
Pin Function
1,2 +12V
10 +3.3V
6,7,9 Ground
3,4 MIX_MO-Mixing Signal
2. Probe Signal Interface
64 / 120
J4
1
1
2
2
CON2
3. Mixing Motor Interface
J6
MIX_MO
1
+12V 2
Header 2
Chapter 7 Debug
Service engineer can check if the probe, disk, fluid pipeline, optical path of iMagic series auto
biochemistry analyzer are normal, and modify motion parameters, and execute individual or
combined command by debugging in software.
For iMagic series, it need to log in with advanced user and then can do debugging under
maintenance interface.
7.1 Debugging of Optical Assembly
Before debug optical assembly, the followings should be done firstly.

l Take out reaction tray.
l Cover the reaction tray lid.
l Parameters of filter wheel are correct.
7.1.1. Confirm Filter wheel Assembly Parameters
Filter wheel assembly parameters need to confirm are [filter wheel wavelength interval] and [the
maximum micro step number of one round of filter wheel]. The following table is the parameter
value of current M7 filter wheel assembly.
Parameter Name Parameter Value
filter wheel wavelength interval 646(demo machine)/711(trial produce)
the maximum micro step number of one round 5818(demo machine)/6400(trial
of filter wheel produce)
Turn on successively the software interface 【Device】-【checking】-【query parameter】, and then

select ‘filter wheel’, and select the parameters in above table, and click button【query parameter】,
65 / 120
then check the parameter value in the top right corner. If the query parameter value differs from
the value in above table, please adjust the parameter value of the table and recheck after
configuration.
7.1.2. Filter wheel assembly movement confirmation
a) Open the filter wheel assembly cover.

b) Turn on successively the software interface 【Device】-【Checking】-【reset】, and then
click button select ‘filter wheel’ from assembly name textbox, and select 【0】from axial
number, then click 【mechanical reset】and observe if the filter wheel are making
mechanical reset movement counterclockwise. If it moves clockwise, there may be some
problem with the motor cable. Close reset interface after finish reset.
66 / 120
Note:
1. the sign of sucessful mechanical reset: the 340nm wavelength filter shall stay in
position of light aperture if ther mechanical reset finish regardless of which position the
filter wheel starts. Refer to the following position with red mark.
Blank
filter
2. The 340nm filter is the next filter to blank filter clockwise.
7.1.3. Fix Lamp Assembly
Adjustment target: fix lamp assembly at proper position and make sure that the light signal
reception board can get the strongest signal.
Adjustment steps:
a) Fix lamp on the lamp holder, as shown in the figure.
67 / 120
b) Fix the filter wheel assembly cover on the support and fix the lampholder on the cover. Do
not fix tightly. Make mechanical reset for filter wheel, and adjust the lampholder position
after finish reset. Test the output voltage value of singal reception board by
multimeter(test point is J2-3 on signal board, refer to the figure）。Fix the lampholder until
the value of multimeter reaches to the maximum(generally the voltage can be adjusted to
0.8v~1.6v）。
Lamp holder
adjustment
position
Signal reception
board. Output
voltage test point
c) Dismantle cover, reinstall the lamp cover and lid into the support.
Note: The voltage may be over 1.6V for the reason of lamp filament position change. Then
adjust the direction of lamp to correct.
7.1.4. Debugging of Filter Wheel Specific Position
Don’t debug filter wheel specific position until lamp is well fixed. The debugging parameters of filter
wheel specific position are including below:
Ø Filter wheel initial position
Ø Position of wavelength 1 of the filter wheel
After debug the [Filter wheel initial position], [Position of wavelength 1 of the filter wheel] will
update automatically. No need re-debugging.
68 / 120
Debug target: after filter wheel initializing, 340nm filter will move to optics aperture and the signal
of 340nm wavelength received by signal reception board is the strongest.
Debug step:
a) Test output voltage of signal reception board by multi-meter. (test point is J2-3 on the signal
board, as shown in the figure)
b) Micro adjusts the position of 340nm wavelength. Turn on successively the software interface
【Device】【 - Checking】【 - movement operation】, and then select ‘filter wheel’ from assembly name
textbox, and select 【0】from axial number, then input micro adjust step number under
【steps/coordinate】button (the steps can be positive or negative, positive means rotate
anti-clockwise and negative means rotate clockwise). Click 【defined movement steps】button, and
observe the voltage value of signal reception board, then record the maximum value after the
output voltage comes to the maximum. (Generally the voltage can be adjusted to 0.8v-1.6v) .
c) As the above figure show, select [Filter Wheel initial position] under the drop-down list of
[Spec position ID] in the area of [Move to spec position], and click 【save as spec position
coordinate】 to save.
69 / 120
d) Confirm filter wheel specific position. Under the interface of 【reset】, make mechanical
reset for filter wheel repeatedly. And observe if the output voltage value of signal reception board
is the same as that of step b micro adjustment. If it is not the same, repeat step b and c. On the
contrary, filter wheel specific position adjustment finish.
7.1.5. Debugging of Filter Wheel Gain
Debug target: While filter wheel switch to different wavelength, signal processing board output
voltage is approaching to the maximum value (about 8.2v~8.3v). (AD value is 53000~55000)
Debug step:
a) Turn on the interface of [reset], make mechanical reset for filter wheel.
b) turn off the interface of [reset], and turn on [A/D reading], and then click [reading] to get the
current AD value of the wavelength 1~8 (the ideal AD value of each wavelength shall be among
53000~55000, which can be adjusted by adjusting the amplification gain of each wavelength.)
c) Adjust the amplification gain of each wavelength, take wavelength 1 as an example, if the AD
value of wavelength 1 is above 55000, then we shall reduce the amplification gain of wavelength 1.
Operation steps: turn on the interface of [query parameter], and select [reaction disk wavelength 1
gain] , and click [query parameter], and then reduce the wavelength 1 parameter value, and then
click [configuration parameter] to save the updated wavelength 1 parameter value.
d) Confirm if the debugging of amplification factor is correct. Turn on the interface of [A/D reading],
and click [reading] button, and observe if the AD value of wavelength 1 is reduced after reading.
Repeat step c operation and ensure the AD value of wavelength 1 is between 50000~55000, then
the gain adjustment of wavelength 1 is finished. If the result is out of the range, there may be the
problem of signal processing board, or the parameter of [filter wavelength interval], [filter wheel
maximum micro step of one round] are wrong.
e) Debug the other wavelength amplification gain as per the same steps.
70 / 120
Note:
1. The gain parameter of different wavelength can be adjusted and confirmed at the same time.
2. If it always be out of the allowable range, it maybe the problem of signal conditioning board, or
the parameter problem of [filter wheel wavelength interval] and [maximum micro steps of one
round of filter wheel] of filter wheel.
3. There is a precondition if the filter wheel moves to specific wavelength position, namely, it must
be moved from wavelength 1 to 8 in turn, without any skip. For example, if you want to move the
filter wheel to wavelength 7, the correct step is making mechanical rest for the filter wheel (move to
wavelength 1), and then move in turn to wavelength 2, 3, 4, 5, 6, and 7. You cannot move to
wavelength 7 directly after mechanical reset.
7.1.6. Lamp Information
Now the machine adds the function of halogen tungsten lamp service time statistic. The halogen
tungsten lamp service time statistic shall be zero before put in storage.
Operation steps as below：
a) Under the interface of 【Device】-【Checking】，turn on 【lamp information】 interface；
b) Click 【clear】button, and clear the halogen tungsten lamp service time statistic；
c) Click 【query】button, the halogen tungsten lamp service time shall be 0；
Note: 1. Make sure do 【clear】operation after change new halogen lamp;
2. the function is only applicable for the upper computer version of 1.0.5.3 or above.
3. the function is only applicable for the lower computer version of 00020006 or above.
7.2 Debugging of Reaction Tray Assembly
l There is a precondition to debug reaction tray assembly, namely, washing arm assembly
should stay at home position.
71 / 120
7.2.1 Confirmation of Reaction Tray Assembly Parameters
The parameters of reaction tray assembly need to confirm are as below:

Reaction tray cup interval 400
Reaction tray target temperature 370
Reaction tray maximum micro-step of one round 19200
Reaction tray AD compensation value 0
Reaction tray leakage detection 0(No)/1(Yes)
Turn on successively “Device” – “Checking” – “query parameter” interface, and then select
“reaction tray” from assembly name, and then select corresponding parameter name to check the
parameter value. If they are different from the values in the above table, please configurate the
values as per the above table. And then query again to confirm.
7.2.2 Debugging of Reaction Tray optocoupler position
Debug target: ensure the time of reaction cuvettes passing the light source and code disk gap
detection is synchronous.
Debug steps:
a) Make mark in the light source position of incubation disk in the reaction tray assembly. Then
install reaction tray, and screw up reaction nut. Take off reaction tray motor cable, but switch on
the machine, then manually move the reaction tray and ensure the reaction cuvettes edge are
aligning with the mark position of light source.
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Cup edge
Light source
center
b) Debugging of Reaction Tray optocoupler position. Keep the code disc position static, take away
reaction tray, test optocoupler signal by multimeter (test point is J3-2 on the step motor connection
board, or the TP test point beside J3). Move left and right the reaction tray position, and test the
voltage change by multimeter. Fix reaction tray optocoupler if the voltage change from 3.3V to
1.0V or so.
Test
point
c) Confirm if the reaction tray position debugging finish. Install reaction tray, rotate it by hand
and confirm that the optocoupler signal is changing from 3.3V to 1.0V while cuvettes border
passing the light source position. If it is ok, the debugging of reaction tray optocoupler position
debugging finish. If it is not ok, debug again.
7.2.3 Debugging of Reaction Tray Specific Position
The debugging parameters of reaction tray specific position are as below:

Ø Reaction tray 1st cup position
Ø Reaction tray initial position
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[Reaction tray 1st cup position] will update automatically if [reaction initial position] debugging
finish.
Debug target: adjust the reaction tray 1st cup position right under sampling position.
Debug steps:
a) Dismantle the washing head of the washing arm, and take away reaction tray. Cover one gap of
the reaction tray code disk with a copper sheet, the covered gap is gap 0, and then count to gap 8
clockwise, and cover the gap 8. Install the reaction tray, and tighten up the reaction tray nut, and
close the reaction tray cover.
b) turn on successively the interface [device]- [Checking]-[reset], and then select [reaction tray]
from assembly name, axis number [0], and click [mechanical reset] button, then close reset
interface after the reaction tray mechanical reset finish.
c) Micro-adjust reaction tray position and ensure the 1st cuvettes is right under the sample
position. Open the “movement operation” interface, select “reaction tray” from assembly name
and axis number “0”, and input step number in “step/coordinate” column.(step number can be
positive and negative, positive means reaction tray move anticlockwise, and negative means
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reaction tray move clockwise) . Click “defined movement steps” button, and finish micro step
number adjustment. When the reaction tray 1st cup is adjusted to right under sampling
position, select “reaction tray 1st position” from “specific position number” and click “save as
specific coordinate” to finish the adjustment of reaction tray 1st cup position.
a) Select “reaction tray initial position” from “specific position ID” column, and click “save as
specific coordinate” button to finish the debugging of reaction tray initial position.
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Close “movement operation” interface, and open “reset” interface, and remake mechanical
reset for reaction tray. If the 1st cup is right under the sampling position after the reset finish, it
means the reaction tray specific position debugging finish; if it is not ok, continue steps b-c.
7.2.4 Debugging of reaction tray offset
Debug target：Make sure the reaction cuvettes stop right under the sampling position after the
reaction tray move to a specific cuvettes position.
Reaction cuvettes position offset refers to the micro-step numbers between the recent code
disk gaps detected by optocoupler in the reaction tray to the stop position when the reaction
cuvettes stop right under the sampling position.
Debug steps:
a) Get the current machine offset。 Query and record the parameter of [reaction cuvettes
offset] in the “query parameter” interface.
b) Verify if the configuration is correct. Remove the wash head of wash arm, and
close the reaction tray. Click [drain up] button on [daily maintenance] interface. Observe if
the reaction cuvettes are right under the sampling position everytime the reaction tray
moves one cuvettes. If yes, it means the offset don’t need re-configuration. Stop the drain
up operation after the reaction tray runs for one cycle. If it is not right under the sampling
position, stop the drain up operation, and re-configurate the offset.
c) Re-configurate offset. Re-configurate 【reaction tray offset】parameters on the

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interface of query parameter. Verify as per step b after configuration finish. Do not stop
until the verification is correct.
Note：If some cuvettes are right under the sampling position, and some cuvettes are not
right under the sampling position during the drain up operation, it means the code disk of
reaction tray is marked unevenly. If the reaction cuvettes are located in anticlockwise position
of the sampling position, it means the offset value is too big, otherwise, it is small.
7.2.5 Debugging of reaction tray temperature
Preparation steps：
a) add water to reaction cuvettes；
b) put the thermometer sensor into the reaction cuvettes filled with water. The
sensor cannot contact with cuvettes wall；
c) put the thermometer sensor into the reaction cuvettes filled with water, and the
sensor cannot contact the reaction cuvettes wall, and then observe the change of
temperature. If the change is less than 0.1℃（about 30min）, record the temperature value;
if the temperature value is 37±0.1℃, the temperature is ok.
d) If the actual temperature of reaction cuvettes is lower, it needs set the [reaction
tray temperature modification value] parameter; if the actual temperature is higher, the
modification value is negative, if the actual temperature is lower, the modification value is
positive. The modification value is 10 times the actual modification temperature. For
example, the modification value shall be 15 if it needs to modify 1.5℃；
7.3 Debugging of Ram Pump Assembly
7.3.1 Confirmation of Ram Pump Assembly Parameters
The ram pumps parameters need to confirm are as below:

Syringe micro step number per ul 566( icubio )/640( Keyto )
Syringe reverse compensation interval 4
Syringe retract volume 4
Turn on successively [Device]-[Checking]-[query parameter] interface, select [syringe] from
assembly name, and then select corresponding parameter name and click [query parameter] to
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observe the values in parameter values column. If the values are different from the above table, it
needs to configurate to the values shown in the table. And then re-query and check.
7.3.2 Confirmation of Ram Pump Assembly Motion Function
Turn on successively [Device]-[Checking]-[reset] interface, select [syringe] from assembly

name, and then select axis number [0], and click [mechanical reset] button to observe if the ram
pump make mechanical reset. Close the reset interface after the reset finish.
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7.4 Debugging of Wash Arm Assembly
The preconditions of debugging wash arm assembly are as below:

l Debugging of reaction tray assembly finish.
l Reaction tray initializing finish.
7.4.1 Confirmation of Wash Arm Assembly other Parameters
The parameter values of wash arm assembly need to confirm are as below:
Parameter name Parameter Value
Waste liquid aspiration time 1625
Wash time 925
Wash position steps 800
Wash well position mode 0(wash well position is zero position of
Y axis.)/1(wash well position is zero
position of both X and Y axis)
Wash probe steps 0(4 steps)/1(6 steps)
Back-aspiration time 400
Peristaltic pump 0(other machine)/1(with peristaltic
pump)
Turn on successively [Device]-[Checking]-[reset] interface, select [wash mechanism] from

assembly name, and then select “waste liquid aspiration time” and “wash time”, and click [query
parameter] button to observe the parameter value on the top right corner. If the parameter value is
not the same as that in the above table, it needs configurate to the values in the above table. And
then re-query and confirm.
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Note: instructions for wash well position mode:
There are two versions for the X-axis zero position.
Version 1: X-axis zero position is in sampling position, namely wash well position mode is 0;
Version 2: X-axis zero position is in wash well position, namely the wash well position mode is 1;
The two versions are depending on machine glass panel, if the wash well position on the glass
panel is on the left of sampling position, the wash well position mode is 0; if the wash well position
on the glass panel is on the right of sampling position, the wash well position mode is 1.
7.4.2 Confirmation of Wash Arm Assembly Motion Function
Turn on successively [Device]-[Checking]-[reset] interface, select [wash mechanism] from

assembly name, and then select axis number [0], and click [mechanical reset] button to observe
if the wash arm make mechanical reset. Close reset interface after reset finish.
7.4.3 Fix Wash Arm Assembly
Debug Target: after reaction tray reset, the wash probe should be right above the reaction
cuvettes.
Debug step:
a) Make mechanical reset for wash arm.
b) Make mechanical reset for reaction tray, and fix the position of cuvettes.
c) As show in the figure, unscrew the four screws under the wash arm, and move the wash arm
assembly left and right ensure the wash probe is located in the middle of reaction cuvettes. And
then fix the screws.
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Wash Arm (left side) Wash Arm (right side)
d) As shown in the figure, unscrew the 2 screws above the wash head, and move the wash head
back and forth, ensure the wash probe is in the middle of reaction cuvettes.
e) Fixation of wash arm finish.
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7.4.4 Debugging of Wash Arm Assembly specific Position
The parameters of wash arm assembly specific position need to debug include:
a) Wash mechanism reaction cuvettes base position
b) Wash mechanism reaction cuvettes downward wash position.
【wash position underneath reaction cuvettes】will update automatically after【Wash
mechanism reaction cuvettes base position】debugging finish. If 【wash position
underneath reaction cuvettes】are not proper, it can be adjusted separately.
Debug steps：
a) as shown in the figure, the wash probe must be fixed tightly.
b) as shown in the figure, the 6 wash needles head must be horizontal, but the 2 single needle can
be a little bit longer.
Wash needle
fix position
Needle head shall

be horizontal
Debug target: ensure the reaction cuvettes can be clean up and drain up when the reaction
cuvettes pass by the wash station.
Debug step:
a) Make mechanical reset for the reaction tray.
b) Make mechanical reset for the wash station.
c) turn on “motion operation” interface, and select “wash mechanism” from assembly name, and
axis ID 【0】, and click the drop-down list of [Specific position number] column, and then select
“wash mechanism reaction cuvettes base position”, and then click “move to specific position”
button, the wash arm will move to reaction cuvettes base.
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d) Input step number in “step/coordinate” column (the micro step can be positive and negative,
positive means wash arm shall be adjusted downward, negative means wash arm shall be
adjusted upward), click “defined motion step” button to adjust. Meanwhile, observe the drain up
needle (the left single needle). Stop adjustment when the drain up needle was raised up slightly by
the cuvettes. And click “save as specific coordinate” button to save parameters of “wash
mechanism reaction cuvettes base position”.
e) Input -800 in “step/coordinate” column(wash arm move upward 800 micro steps), and click
“defined movement steps” button, click the drop-down list of [Specific position number] column,
and then select “wash mechanism reaction cuvettes downward wash position”, and then click
“save as specific coordinate” button to save the parameters of “wash mechanism reaction cuvettes
downward wash position ”.
f) Confirmation of wash arm specific position. Make mechanical Reset for wash arm under “reset”
interface, and run the operation of wash arm moving to wash mechanism reaction cuvettes base
position under the “movement operation” interface. Then observe if it is accordance with
adjustment result (drain up needle rise up slightly). If it is accordance, the adjustment of wash
mechanism reaction cuvettes base position finish. If it is not, continue adjustment. Run the
operation of moving the wash arm to wash mechanism reaction cuvettes downward wash position,
and observe if move to the preset position. If yes, the adjustment finish; if no, continue adjustment.
7.5 Debugging of sampling arm
The precondition for debugging sampling arm:

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l Debugging of reaction tray assembly finish
l Reaction tray finish initializing(when cleaning pool position mode is “0” should adjust
X-axis optocoupler shelter position in order to adjust X-axis optocoupler shelter position)
l Trough E loading (in order to adjust the Y axis optocoupler shelter position)
l The sampling needle shall be above machine panel after Z-axis back to zero. (in order to
adjust Z-axis optocoupler shelter position)
7.5.1 Confirmation of sampling arm Parameters
Below table is the parameter value of sampling arm set.

S needle inner wall wash time 1125
S needle outer wall wash time 1125
S needle descending depth after 10
detecting liquid level
R1 needle descending depth after 70
R2 needle descending depth after 50
Air dose aspirated by S probe before S 4
aspiration
Excess aspirating volume aspirated by 4
S probe while aspirating S
Stirring time 3250
Sampling mixing duty cycles 3
Cleaning mixing duty cycles 4
stirring step 940
needle wash step 800
R1 limitation step 3100
R2 limitation step 2100
S limitation step 2650
distance between slot A and D 2365
distance between slot B and D 1645
distance between slot C and D 725
distance between slot E and D -945
distance between slot F and D -2035
distance between slot G and D -2715
distance between slot H and D -3495
period fix time 6500 (100T/H) 5100(120T/H)
reagent rack/reagent bottle base step 3100 (apply to bottom version above
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00020008)
reagent rack standard cup base step 2100 (apply to bottom version above
00020008)
reagent rack micro cup base step 1 1650 (apply to bottom version above
00020008)
reagent rack blood tube base step 2 2750 (apply to bottom version above
00020008)
sample rack standard cup base step 2650 (apply to bottom version above
00020008)
sample rack micro cup base step 1 2200 (apply to bottom version above
00020008)
sample rack blood tube base step 2 3350 (apply to bottom version above
00020008)
Turn on successively [Device]-[Checking]-[query parameter] interface, select [needle] from

assembly name, and then select corresponding parameters in the above table and check the
parameter values in the top right corner. If it is different from the values in the above table, it needs
to re-configurate to the parameter values in the table and then recheck and confirm.
7.5.2 Debugging of optocoupler shelter position
X-axis optocoupler shelter debugging depend on the cleaning pool position mode
7.5.2.1 Debugging of X-axis optocoupler shelter
Debug target: make sure sampling probe should be right above the reaction tray sampling position
after X-axis back to zero.
When cleaning pool position mode “0”, make sure sampling probe should be right above the
reaction tray sampling position after X-axis
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When cleaning pool position mode “1”, make sure sampling probe should be slant to the right
of cup edge position.
Near to the
the right
side of cup
Debug step:
a) make sure the Z-axis optocoupler shelter block the zero position of Z-axis; and then take away
Z-axis motor cable;
b) Switch off the machine, move manually the sampling needle to the position that right above
sampling positive.
c) Switch on the machine, and turn on “reset” interface, make mechanical reset for wash arm and
reaction tray.
d) Select “needle” from assembly name button on “reset” interface, and select X-axis from axis
number, and then click “zero position calibration”. After calibration, manually move the Z-axis to
the reaction cuvettes position, and observe if the sampling needle is right above the reaction
cuvettes. If it is not in the right above position, manually move the Z-axis back to zero position
optocoupler position, and redebug the X-axis optocoupler shelter position.
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e) debugging of X-axis optocoupler shelter. As shown in the figure. Unscrew adjustment
screws of X-axis shelter, slighly move optocoupler shelter left and right (if the needle close to near
optocoupler, move the optocoupler shelter right; if the needle close to distance optocoupler, move
the optocoupler left), and fix screws, and make zero positon operation for x-axis.
Holder fix screws Holder
f) repeat step e, until the sampling needle is adjusted to the right above positon of cuvettes.
7.5.2.2 Debugging of Y-axis optocoupler shelter
Debug target: make sure the Y-axis direction of the sampling needle is located right above the first
position of slot E, as shown in the figure.
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Debug step:
a) make sure the Z-axis optocoupler shelter block the zero position of Z-axis; and then take away
Z-axis motor cable;
b) switch off the machine, move manually the sampling needle to the position that right above the
1st position of slot E.
c) switch on the machine, select “needle” from assembly name button on “reset” interface, and
select Y-axis from axis number, and then click “zero position calibration”. After calibration,
manually move the Z-axis to the reagent bottle position, and observe if the sampling needle head
is right above the reagent bottle. If it is not in the right above position, manually move the Z-axis
back to zero position optocoupler position, and redebug the Y-axis optocoupler shelter position
d). debugging of Y-axis optocoupler shelter. As shown in the figure. Unscrew the adjustment
screws of Y-axis shelter, slighly move optocoupler shelter left and right (if the needle close to near
optocoupler, move the optocoupler shelter ahead; if the needle close to distance optocoupler,
move the optocoupler backward), and fix screws, and make zero positon operation for Y-axis.
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Adjustment
Adjustment Screw
Screwfor Y
for Y Axis Blanks
Axis Blanks
e) Repeat step d, until the sampling needle is adjusted to the position right above the first positon
of slot E.
7.5.3 Probe Special Position Adjustment
Probe special position includes initial position, clean position, sample position, reagent position,
and reaction tray sampling position. And each one involves the x, y, z three directions of
debugging, relatively cumbersome and complex.
7.5.3.1 Initial Position and Clean Position
The specific position parameter related to sampling arm initial position and cleaning position
include:
Ø X axis initial position
Ø X axis cleaning horizontal position
Ø Y axis initial position
Ø Y axis cleaning horizontal position
Ø Z axis cleaning pool upside position
Ø Z axis cleaning pool downside position
At present the design of the M7 initial position and cleaning position are the same one, so only
need to set-up a good 【X axis initial position】 and 【 Y axis initial position】, then【X axis cleaning
level position 】 and 【 Y axis cleaning level position 】will automatically finish updating.
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Calibration target: adjust the X axis and Y axis direction of sampling probe to move right above the
clean cell, then the Z axis direction can move directly to the clean cell bottom 2~3mms. As shown
in the picture below:
X Axis direction adjustment

When the clean cell position mode is 0:
The adjustment steps are as below:
A) On the interface of 【Device】【Checking】【Reset】, do mechanical resetting for probe X axis
and do home position calibration for probe Y axis respectively.
B) Fine-tune the X axis right above to the clean position direction. In 【Device】-【Checking】-
【Movement operation】 interface, choose the parts name 【Probe】,axis number【X axis】,
in the column of 【steps/ coordinate 】 write the steps number needed to fine-tune, (fine-tune
steps could be positive or negative, the positive means the x axis is far away from x axis
home position sensor position, the negative means the x axis is close to the x axis home
position sensor position), click on the button 【movement steps specified 】 for fine-tuning, at
the same time, observe the sample probe, until the sampling probe has been fine-tuned right
above to the clean cell X axis direction, then stop fine-tuning.
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C) Save the X axis specific position. Choose axis number 【X axis】, select 【X axis initial
position】 in the drop-down menu of 【specific position number】, then click 【save as particular
coordinate】 button to save.
D) Confirmation of X axis specific position. First do mechanical reset for x axis in the 【reset】
interface, then in the 【movement operation】 interface choose respectively the 【x axis initial
position】 and 【x axis clean level position】 ,and click on the button 【move to a specific
position】 to confirm whether x axis has moved right above the clean cell. If yes, then
debugging is completed, otherwise, should continue to fine tune.
When the clean cell position mode is 1: X axis initial position and clean position are at home
position.
Adjustment steps:
a) On the interface of【Query parameter】, choose parts name 【probe】, configure【X axis initial
position】 and 【X axis clean cell horizontal position】, the parameter should be 0.
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Y Axis direction adjustment
Since the clean position and initial position are the home position of Y axis, so only need to
configure【Y axis initial position】 and 【Y axis clean cell level position】on the interface of 【Query
parameter】to be 0 will be good.
Z axis direction adjustment

T he specific position of Z axis clean cell position should be adjusted to the position up to Z aixs
clean cell and the Z axis clean cell downside position.
Adjustment steps:
a) Confirm that the sample probe is right above the clean cell. If not, then move the sample probe
to be right above the clean cell on the interface of 【Movement Operation】.
b) Do Mechanical reset for probe Z axis on the interface of 【Reset】.
c) Flat tuning. On the interface of 【Movement Operation】, choose the parts name 【Probe】, axis
number 【Z axis】,then write the flat tuning steps 200 in the steps/ coordinate column (Z axis
move 200 micro steps down
d) Fine tuning. In the column of 【steps/ coordinate 】 write the steps number needed to fine-tune,
(fine-tune steps could be positive or negative, the positive means the Z axis move downwards,
the negative means the Z axis moves upwards), click on the button 【movement steps specified 】
for fine-tuning, at the same time, observe the sample probe, until the sampling probe tip has been
fine-tuned right at the clean cell level, then stop fine-tuning.
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e) Save the Z axis cleaning station related specific position coordinates. In the drop-down menu of
the【particular position number 】 column to selec 【Z t axis cleaning cell above position】, and then
click 【save for a specific position coordinates 】button to save.
f) Confirmation of Z axis specific position. First do mechanical reset for Z axis in the 【reset】
interface, then in the 【movement operation】 interface to choose parts name 【Probe】 and axis
number 【Z axis】, choose the【Z axis clean cell above position】in the drop-down menu of the
【specific position number 】column, and click on the button 【move to the specific position】 to
confirm whether the probe tip has moved to the cell horizontal position. If yes, then the adjustment
has finished, otherwise, need to continue to adjust. choose the【Z axis clean cell below position】
in the drop-down menu of the【specific position number 】column, and click on the button 【move
to the specific position】 to confirm whether the sampling probe will run into the bottom of the clean
cell or not, if yes, then need to separately fine-tune the 【Z axis clean cell below position】
coordinates.
7.5.3.2 Sampling Position
The premise of sampling position adjustment must be the reaction tray has good set-up, and has
completed the initialization.
At present M7 design distinguishes the positions by S adding position, R1 adding position and R2
adding position, actually the three positions are the same position. So, after adjusted the position,
need to save at the same time S adding position, R1 adding position and R2 adding position.
For the sampling position, those specific position parameter related to adjustment include:
Ø X axis reaction tray S adding position
Ø X axis reaction tray R1 adding horizontal position
Ø X axis reaction tray R2 adding horizontal position
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Ø Y axis reaction tray S adding position
Ø Y axis reaction tray R1 adding horizontal position
Ø Y axis reaction tray R2 adding horizontal position
Ø Z axis reaction tray sampling position above position
Ø Z axis reaction tray sampling position below position
At present only need to well adjust 【X axis reaction tray adding S position】 and
【Y axis reaction tray adding S position】, and then the following positions coordinate will
automatically update: 【X axis reaction tray adding R1 position】【X axis reaction tray adding R2
position】【Y axis reaction tray adding R1 position】【Y axis reaction tray adding R2 position】,
after well adjusted 【Z axis reaction tray sampling position above position】, 【Z axis reaction tray
sampling position below position 】coordinate will update automatically.
Calibration target: adjust the X axis and Y axis direction of sampling probe to move right above the
sampling position, then the Z axis direction can move down directly to reaction cup rim position
below to sampling position and 1mm far away from the reaction cup bottom. As shown in the
picture below:
l X axis direction adjustment

Since X axis sampling position is also the home position of X axis, thus, the sampling position of x
axis have already been well adjusted when the x axis sensor block slice is adjusting, only need to
do home position calibration for x axis, then save the current coordinate as the samping position
coordinate.
Adjustment steps:
When the clean cell position mode is 0: X axis sampling posiiton is home position.
Adjustment steps:
a) On the interface of【Query parameter】, choose parts name【probe】, configure【X axis reaction
tray S adding position】,【X axis reaction tray R1 adding horizontal position】and 【X axis reaction
tray R2 adding horizontal position】, the parameter should be 0.
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When the clean cell position mode is 1:
Adjustment steps:
a) On the interface of【Device】-【Checking】-【Reset】, do mechanical reset for probe
Z axis and Y axis in turn, then do home position adjustment for X axis.
b) On the interface of【Movement operation】, choose parts name【probe】, axis number
【Y axis】, then choose【Y axis reaction tray S adding position】 in the drop-down
menu of 【specific position number】column, then click the button【move to the
specific position】to move the Y axis to the sampling position.
c) Flat tuning. On the interface of 【Movement operation】, choose parts name【probe】,
axis number 【X axis】, then choose【X axis reaction tray S adding position】 in the
drop-down menu of 【specific position number】column, then click the button【move
to the specific position】to move the probe X axis direction to the sampling position.
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d) Fine-tuning. In the column of 【steps/ coordinate 】to write the steps number needed
to fine-tune, (fine-tune steps could be positive or negative, the positive means the x
axis is moving far away from x axis home position sensor position, the negative
means the x axis is moving close to the x axis home position sensor position), click
on the button 【movement steps specified 】 for fine-tuning, at the same time,
observe the sample probe, until the sampling probe has been fine-tuned right above
to the reaction cup below to sampling position, then stop fine-tuning.
e) Save X axis sampling station related specific position coordinate. Then choose【X
axis reaction tray S adding position】 in the drop-down menu of 【specific position
number】column, then click the button【save as the specific position coordinate】to
save.
f) Confirmation of Y axis sampling position related specific position. First do
mechanical reset for Y axis in the 【reset】 interface, then in the 【movement
operation】 interface to choose parts name 【Probe】 and axis number 【Z axis】,
choose the 【x axis reaction tray S adding position】, 【x axis reaction tray R1 adding
position】【x axis reaction tray R2 adding position】in the drop-down menu of the
【specific position number 】column, and click on the button 【move to the specific
position】 to confirm whether the probe X axis direction has moved to right above the
reaction cup below to sampling station. If yes, then the adjustment has been finished.
Otherwise, need to continue to adjust.
Y axis direction adjustment
Adjustment steps:
a) On the interface of【Device】-【Checking】-【Reset】, on mechanical reset for
probe Z axis and Y axis in turn, then do home position adjustment for X axis.
b) On the interface of 【Movement operation】, choose parts name【probe】, axis
number 【Y axis】, then choose【X axis reaction tray S adding position】 in the
drop-down menu of 【specific position number】column, then click the button
【move to the specific position】to move the X axis to the sampling position.
c) Flat tuning. On the interface of 【Movement operation】, choose parts name
【probe】, axis number 【Y axis】, then choose【Y axis reaction tray S adding
position】 in the drop-down menu of 【specific position number】column, then
click the button【move to the specific position】to move the probe Y axis direction
to the sampling position.
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d) Fine-tuning. In the column of 【steps/ coordinate 】 to write the steps number
needed to fine-tune, (fine-tune steps could be positive or negative, the positive
means the Y axis is moving far away from Y axis home position sensor position,
the negative means the Y axis is moving close to the Y axis home position sensor
position), click on the button 【movement steps specified 】 for fine-tuning, at the
same time, observe the sample probe, until the sampling probe has been
fine-tuned right above to the reaction cup below to sampling position, then stop
fine-tuning.
e) Save Y axis sampling station related specific position coordinate. Then choose
【Y axis reaction tray S adding position】 in the drop-down menu of 【specific
position number】column, then click the button【save as the specific position
coordinate】to save.
f) Confirmation of Y axis sampling position related specific position. First do mechanical reset for Y
axis in the 【reset】 interface, then in the 【movement operation】 interface to choose
parts name 【Probe】 and axis number 【Z axis】, choose the 【Y axis reaction tray S
adding position】, 【Y axis reaction tray R1 adding position】【Y axis reaction tray R2
adding position】in the drop-down menu of the【specific position number 】column, and
click on the button 【move to the specific position】 to confirm whether the probe Y axis
direction has moved to right above the reaction cup below to sampling station. If yes,
then the adjustment has been finished. Otherwise, need to continue to adjust.
Z Axis direction Adjustment
a) Make sure that the sampling probe is right above the reaction cup below to the
sampling station. If not, then move the sampling probe to the right above position on the
interface of 【movement operation】.
b) Do mechanical reset for Z axis on the 【reset】 interface.
97 / 120
c) On the 【movement operation】 interface to choose parts name 【Probe】 and axis
number 【Z axis】, in the column of 【steps/ coordinate 】 to write the steps number
needed to fine-tune, (fine-tune steps could be positive or negative, the positive means
the Z axis moves downwards, the negative means the Z axis moves upwards), click on
the button 【movement steps specified 】 for fine-tuning, at the same time, observe the
sample probe, until the sampling probe has been fine-tuned right at the reaction cup rim
postion, then stop fine-tuning.
d) Save Z axis reaction tray sampling position above position. Then choose【Z axis
reaction tray sampling position above position】 in the drop-down menu of 【specific
position number】column, then click the button【save as the specific position coordinate】
to save.
e) Confirmation of Z axis sampling position related specific position. First do mechanical
reset for Z axis on the 【reset】 interface, then in the 【movement operation】 interface
to choose parts name 【Probe】 and axis number 【Z axis】, choose respectively the 【Z
axis reaction tray sampling position above position】, 【Z axis reaction tray sampling
position below position】in the drop-down menu of the【specific position number 】column,
and click on the button 【move to the specific position】 to confirm whether the probe
move to the cup rim position and will not run into the bottom of the cup. If yes, then the
adjustment has been finished. Otherwise, need to continue to adjust.
7.5.3.3 Reagent/ Sample Position
Reagent / sample positions area are divided by 8 grooves: A, B, C, D, E, F and G. So the position
needed to adjust are: 10# for groove A, # 10 for groove B, # 10 for groove C , # 10 for groove D, #
10 for groove E, # 10 for groove F, # 10 for groove G, # 2 for groove H and all types of bottom
position of sample container. The detailed position on the machine is shown as below picture.
98 / 120
D groove 1#
A groove 10# C groove 10# E groove 10# H groove 2#
B groove 10# D groove 10# G groove 10#
F groove 10#
The specific positoon paramter of reagent position related to the adjustment includes:
Groove A position
X axis 10# at groove A horizontal position.
Y axis 10# at groove A position.
Groove B position
X axis 10# at groove B horizontal position.
Y axis 10# at groove B position.
Groove C position
X axis 10# at groove C horizontal position.
Y axis 10# at groove C position.
Groove D position
X axis 10# at groove D horizontal position.
X axis 1# at groove D horizontal position.
Y axis 10# at groove D position.
Z axis D groove cup rim above position.
Groove E position
X axis 10# at groove E horizontal position.
Y axis 10# at groove E position.
Groove F position
X axis 10# at groove F horizontal position.
Y axis 10# at groove F position.
Groove G position
X axis 10# at groove G horizontal position.
Y axis 10# at groove G position.
99 / 120
Sample bottom position
Ø Z axis reagent rack reagent bottle bottom position.
Ø Z axis reagent rack standard cup bottom position.
Ø Z axis reagent rack trace cup bottom position
Ø Z axis sample rack blood tube bottom position
Ø Z axis sample rack standard cup bottom position
Ø Z axis sample rack trace cup bottom position
Adjustment of sample bottom position should on PC version 1.0.6 or more, and firmware version
00020008 or more.
At present only need to well adjust 【The horizontal position of X axis 10# position at groove D】,
【The horizontal position of X axis 1# position at groove D】and 【Y axis 10# position at groove D】,
then all the X and Y direction coordinate of reagent and sample position are well adjusted
accordingly. And for the Z axis direction only need to adjust【Z axis cup rim above position at
groove D】, then confirm and fine-tune other position.
7.5.3.3.1 X axis and Y axis direction adjustment
Adjustment target: adjust the sampling probe to right above the 10# reagent bottle at groove D.
a) On the interface of 【Device】【- Checking】【- Reset】, do respectively mechanical reset for probe
X axis and Y axis.
b) On the interface of【Movement operation】, move X axis to 【X axis 10# horizontal position at
groove D】, move Y axis to 【Y axis 10# position at groove D】.
100 / 120
C) Fine-tune X axis and Y axis, then observe sampling probe until the sampling probe has been
adjusted right above the 10# bottle rim position at groove D.
d) Save the specific position parameter related to D groove positions X axis and Y axis. Choose
respectively 【The horizontal position of X axis 10# position at groove D】and 【Y axis 10# position
at groove D】in the drop-down menu of【specific position number】column. Then click the button
【save as specific position coordinate】to save the parameter of 【The horizontal position of X axis
10# position at groove D】and 【Y axis 10# position at groove D】.
e) Confirmation of specific position parameter related to the reagent and sample positions X axis
and Y axis. First do mechanical reset for probe X axis and Y axis on the 【reset】interface, then on
the 【movement operation】 interface move respectively the X axis to【The horizontal position of
101 / 120
X axis 10# position at groove A】, 【The horizontal position of X axis 10# position at groove B】
【The horizontal position of X axis 10# position at groove C】【The horizontal position of X axis 10#
position at groove D】【The horizontal position of X axis 10# position at groove E】【The horizontal
【The horizontal position of X axis 1# position at groove
position of X axis 10# position at groove F】
G】, 【The horizontal position of X axis 2# position at groove H】and move Y axis to【Y axis 10#
position at groove A】, 【Y axis 10# position at groove B】, 【Y axis 10# position at groove C】,
【Y axis 10# position at groove D】, 【Y axis 10# position at groove E】, 【Y axis 10# position at
groove F】, 【Y axis 2# position at groove H】 .
Note: If there are abnormal position, could adjust them separately and save, then the machine
automatically update the wrong position to the relatively benchmark position steps, to ensure that
the next adjustment for the benchmark 10# position at groove D will not repeat the same problem.
7.5.3.3.2 Z axis direction adjustment
At present the machine has newly increased three types of sample container supported: Hitachi
sample cup Φ 16 x38 specifications (standard cup) bottom position, blood tube bottom position,
trace cup bottom position. So increased below positions:
Ø Z axis reagent rack reagent bottle bottom position.
Ø Z axis reagent rack standard cup bottom position.
Ø Z axis reagent rack trace cup bottom position
Ø Z axis sample rack standard cup bottom position
Ø Z axis sample rack trace cup bottom position
Micro cup bottom position is based on the customer actual micro cup bottom. The factory default
location is 450 micro steps higher than the stand cup bottom position and is just above the
standard cup. That means after confirmed the stand cup bottom position, send to -450 micro steps
position.
Blood tube bottom position is based on the customer actual whole blood collecting volume . After
centrifugal, the junction position of dope and serum is just the blood tube bottom position. If set is
at the dope position, then may have the risk of needle blocking. The factory default whole blood is
2 ml. Take the 1 cm depth of dope as standard debugging. That means the position at 350 micro
steps above the reagent rack position blood tube bottom position, after moved to the reagent rack
blood tube bottom position, then send Z axis to the position with -350 micro steps moved. Or move
from the 700 micro steps below sample rack stand cup bottom position to sample rack standard
cup bottom position, send Z axis to the position with 700 micro steps moved.
The Z axis direction only set-up, Z axis close D tank top position 】【 other location steps have
been according to the set of synchronous updating.
Adjustment target: Z axis can move directly to right above the cup rim position of
groove D at Z axis.
Adjustment steps:
a) Confirm that the sampling probe is right above the 10# reagent bottle of groove D.
102 / 120
b) Do mechanical reset for Z axis on the interface of 【Reset】.
c) Open the probe Z axis operation interface on the interface of 【movement operation】, in the
column of 【steps/ coordinate 】 to write the suitable micro steps number, (fine-tune steps could
be positive or negative, the positive means the Z axis moves downwards, the negative means the
Z axis moves upwards), click on the button 【movement steps specified 】to move the Z axis to
adjust the sampling probe to the reagent bottle mouth position.
d) Save the current position as reagent mouth above position coordinates. Choose 【Z axis
groove D cup mouth above position】in the drop-down menu of 【specific position number】, then
click the button 【save as specific coordinate】to save.
e) Specific position confirmation related to Z axis reagent rack station. First, do mechanical reset
for probe Z axis on the interface of 【reset】, then on the interface of【movement operation】to move
respectively the probe Z axis to 【Z axis groove D cup mouth above position】, 【Z axis reagent
rack reagent bottle bottom position】【Z axis reagent rack stand cup bottom position】【Z axis
reagent rack micro cup bottom position】, 【Z axis reagent rack blood tube bottom position】, 【Z
axis sample rack stand cup bottom position】【Z axis sample rack micro cup bottom position】 and
【Z axis sample rack blood tube bottom position】
If there are abnormal position, could adjust them separately and save, then confirm whether the
sampling probe move to the right position. If no, then need to separately adjust the abnormal
station and save, then the machine will automatically update the wrong position to the relatively
benchmark position steps, to ensure that the next adjustment for the benchmark 10# position at
groove D will not repeat the same problem.
,
7.5.3.3.3 Reagent / sample rack slope adjustment
Adjust 1# position horizontal position of X axis groove D.

Before adjust the reagent / sample rack slope, 【10# position horizontal position of X axis groove
D】must be well adjusted at first.
First, reset Y axis.
Then, fine-tune the sampling prove to right above the x direction of【1# position horizontal position
of X axis groove D】.
At last, choose【1# horizontal position of X axis groove D】in the drop-down menu of the【specific
position coordinate】column, then click the button【save as the specific position coordinate】, then
save the parameter of【1# position horizontal of X axis groove D】.
7.5.3.3.4 Confirmation of reagent position in reagent storehouse.
Open the interface of【Reagent】, click the button【multi position check】to open more position
detection interface, click the button【Check】 to detect the reagent position residual in the reagent
storehouse. The sample arm will move to the related reagent position to detect in turn, then can
confirm the accuracy of reagent position.
103 / 120
Note: During the confirmation process of reagent position, some of individual reagent bottle can be
filled with water to confirm the effectiveness of the liquid level detection. If the sample probe reset
rapidly after detected the liquid level, then the liquid level detection function if normal, otherwise, it
is abnormal..
7.5.3.3.5 Confirmation of sample position at F/G/H groove
1, Open the probe operation interface of 【Device】-【Checking】-【movement operation】.

2, Firstly, move the X axis respectively to 【10# position horizontal position of x axis groove F】,
【10# position horizontal position of x axis groove G】and【2# position horizontal position of H axis
groove F】
3, then, move the Y axis respectively to 【2# position of Y axis groove F】, 【2# position of Y axis
groove G】and 【1# position of Y axis groove H】
4, Observe whether the sample probes are respectively right above the groove F 2# cup mouth,
groove G 2# cup mouth and groove H 1# cup mouth, if yes, means the confirmation is successful.
Otherwise, need to reset the interval between the Y axis sample position in sample rack.
7.6 Liquid Path Debugging
7.6.1 Pipe line Confirmation
1, Firstly, make sure that membrane of water pump is not pressed by the pump block, otherwise, it
may hurt the water pump. Shown as below picture:
104 / 120
Secondly, make sure that no connect error of pipe and no bend of light path.
Thirdly, make sure there is enough Deion water in water tank.
Fourthly, make sure that waste liquid is one third lower than the height of the whole waste tank.
7.6.2 Adjustment for Clean cell liquid path
Adjustment target: to make sure that there is enough water pressure for probe exterior and interior
wash, meanwhile, no water overflow or spill.
Adjustment steps:
a) Open the interface of 【device】, 【checking】, 【initialization】 to initialize the analyzer.
b) Open the interface of【switch control】, then open the interface of 【sample probe interior
washing】.
c) Observe the needle cleaning related water pipes for joint leakage phenomenon, and at the
same time observe the needle inside whether there is water injection and the probe outer wall
cleaning water can arrive the clean cell in time or not, but not overflow. The water volumes for
needle outer wall clean are adjusted by the valve connected by the outer wall inlet pipe.
105 / 120
7.6.3 Wash Arm liquid Path Adjustment
Adjustment target: when wash cuvettes, the short wash probe has water to inject and no water
overflow from the cuvettes, the long wash probe and single probe can aspirate cleaning water,
after cleaning mechanism, the water in the cuvettes can be sucked dry, the water pipe joint has no
leakage phenomenon, after the completion of cleaning, cleaning head has no dripping
phenomenon.
a) Open the interface of 【device】, 【checking】, 【initialization】 to initialize the analyzer.
b) Open the interface of 【device】, 【daily maintenance】, then click the button 【wash】 to wash
the instrument cuvettes.
c) Observe whether has achieved the purpose of debugging.

Fault points of wash arm liquid path are as follows:
1, water pump and valve failure.
2, water pump and magnetic valve connection error.
3, Pipes connect error.
4, Pipes bend.
5, Junction box is blocked.
6, leakage of pipe connector.
7.6.4 Deion water- waste liquid level detection
Connect the Deion water- waste liquid sensor, then put the Deion water sensor into the Deion
water barrel, when the Deion water immersed sensor, click on the button【probe cleaning】, there
will have no alarming for Deion water shortage. If the Deion water didn’t immerse the sensor, then
click the button【probe cleaning】, there will have deion water shortage alarming under the testing
software.
Note: 1, this function should be operated on PC version 1.0.5.3 or more.
2, this function only suitable for the firmware version 00020006 or more.
7.6.5 Confirmation of liquid level detection function

When adjust the sampling arm, during the process of reagent position confirmation, some of
individual reagent bottle can be filled with water, confirm the effectiveness of the liquid level
106 / 120
detection function. If the sampling probe reset rapidly after detected the liquid level, then the liquid
level detection function is normal, otherwise, it is abnormal.
Chapter 8 Analysis Method

There are several analysis methods as below:
8.1 End-point method
End-point method, also known as balance method, is the ideal type of analysis. Items
measured will convert into the reaction products over a period of reaction time, when the reaction
reached equilibrium, it can be said to have reached the end of reaction, and the reaction solution
absorbance will no longer change. Analyzer will choose the parameter set point to read the
absorbance values, and then calculate the results. And the End-point method is divided into one
point method and two point methods.
8.2 Two-point method
Fixed time method means to select two metering point on the curve of time and absorbance, both
of which are not reaction initial absorbance nor end absorbance, the absorbance difference
between these two points are used for result calculation, known as the fixed time method.
This analysis can help solve some of the reactions of non-specific problems.
8.3 Rate method (kinetics)
Rate method, also known as continuous monitoring method. It’s used in the determination of
enzyme activity or enzymatic determination of metabolites, during this time to continuously choose
the linear phase absorbance values in the time and absorbance curve, and to calculate the results
based on the linear unit absorbance changing values. The so-called linear phase is each point
differential absorbance values are equal.
107 / 120
Attachment 1: The hardware fault code table
Fault Code Description Trouble shooting

X Axis calibration failure(unable to detect Connect with distributor or
0x01
optical coupler) iCubio
X Axis is not calibrated before Do mechanical resetting for X
0x02
movement/calibrations failure axis by PC software
Connect with distributor or
0x03 X Axis movement is time out
iCubio
0x04 X Axis movement lost steps
iCubio
X Axis terminal emergency stop protection
(Machine self-protection to forbid Reset again for the x axis
0x05
continuing movement to the terminal mechanical position
direction when the X axis is at the terminal)
X Axis home position emergency stop
protection (Machine self-protection to
Reset again for the x axis
0x06 forbid continuing movement to the home
mechanical position
position direction when the X axis is at the
home position)
Reset again for the x axis
0x07 X Axis abnormal (illegal suspension)
mechanical position
Y Axis calibration failure (unable to detect Connect with distributor or
0x11
sensor) iCubio
Y Axis is not calibrated before movement / Reset again for the Y axis
0x12
calibrations failure mechanical position
0x13 Y Axis movement is time out
iCubio
0x14 Y Axis movement lost steps
iCubio
Y Axis terminal emergency stop protection
(Machine self-protection to forbid Reset again for the Y axis
0x15
direction when the Y axis is at the terminal)
Y Axis home position emergency stop
protection (Machine self-protection to Reset again for the Y axis
0x16
forbid continuing movement to the home mechanical position
position direction when the Y axis is at the
108 / 120
home position)
Reset again for the Y axis

0x17 Y Axis abnormal (illegal suspension)
mechanical position
Z Axis calibration failure (unable to detect Connect with distributor or
0x21
sensor) iCubio
Z Axis is not calibrated before movement / Reset again for the Z axis
0x22
calibrations failure mechanical position
0x23 Z Axis movement is time out
iCubio
0x24 Z Axis movement lost steps
iCubio
Z Axis terminal emergency stop protection
(Machine self-protection to forbid Reset again for the Z axis
0x25
direction when the Z axis is at the terminal)
Z Axis home position emergency stop
Reset again for the Z axis
mechanical position
position direction when the Z axis is at the
home position)
Adjust probe manually and
reset to confirm every
0x27 Z Axis collision protection
specified positions normal of
not.
0x28 Z Axis level stop Temporary unrealized

0x29 Z Axis abnormal(illegal suspension)
mechanical position
0x2A Z Axis is not at home position mechanical position and do
the x/y axis related operation
Back to mechanical reset of Z
After adding sample, Z axis is not at zero
0x2B axis, then to X/Y related
position
operations
Syringe calibrations failure (unable to Connect with distributor or
0x31
detect sensor) iCubio
Syringe is not calibrated before movement Reset again for the ram
0x32
/ calibrations failure pump mechanical position
0x33 Syringe movement is time out
iCubio
0x34 Syringe movement lost steps Connect with distributor or
109 / 120
iCubio
Syringe terminal emergency stop
Reset again for the ram
0x35 forbid continuing movement to the terminal
pump mechanical position
direction when the Syringe is at the
terminal)
Syringe home position emergency stop
position direction when the Syringe is at
the home position)
0x37 Syringe abnormal(illegal suspension)
Wash mechanism calibration Connect with distributor or
0x41
failure(unable to detect sensor) iCubio
Reset again for the wash
Wash mechanism is not calibrated before
0x42 mechanism mechanical
movement / calibrations failure
position
0x43 Wash mechanism movement is time out
iCubio
0x44 Wash mechanism movement lost steps
iCubio
Wash mechanism terminal emergency
stop protection (Machine self-protection to Reset again for the wash
0x45 forbid continuing movement to the terminal mechanism mechanical
direction when the wash mechanism is at position
the terminal)
Wash mechanism home position
emergency stop protection (Machine
self-protection to forbid continuing
movement to the home position direction
position
when the wash mechanism is at the home
position)
Wash mechanism abnormal (illegal
suspension)
position
Reset again for the wash arm
0x48 Wash arm is not at home position
mechanical position
Filter wheel calibration failure(unable to Connect with distributor or
0x51
0x52 Filter wheel is not calibrated before Reset again for the filter
110 / 120
movement / calibrations failure wheel mechanical position
0x53 Filter wheel movement is time out
iCubio
0x54 Filter wheel movement lost steps
iCubio
Reset again for the filter
0x55 filter wheel abnormal(illegal suspension)
wheel mechanical position
0x56 Filter wheel rotation is too slow
iCubio
0x57 Filter wheel time sequence lost control
iCubio
Reaction tray calibration failure(unable to Connect with distributor or
0x61
Reaction tray is not calibrated before Reset again for the reaction
0x62
movement / calibrations failure tray mechanical position
0x63 Reaction tray movement is time out
iCubio
0x64 Reaction tray movement lost steps
iCubio
Reaction tray abnormal ( illegal Reset again for the reaction
0x65
suspension) tray mechanical position
0x66 Reaction tray rotate too slow/no rotation
iCubio
0x67 Reaction tray correction failed
iCubio
0x71 Cannot detect liquid level
iCubio
Increase reagent or sample
0x72 Residual volume is not enough
volume
Reaction tray still move while time Connect with distributor or
0x73
sequence started iCubio
0x81 Communication data lost
iCubio
0x82 Communication data do not support
iCubio
0x83 Probe mistake during cycle testing
iCubio
X Axis failed to come back to home Connect with distributor or
0x84
position during periodic testing iCubio
X Axis failed to come back to home Connect with distributor or
0x85
position during periodic testing iCubio
0x90 Coordinate errors of Reaction plate Connect with distributor or
111 / 120
iCubio
0x91 Photoelectric data acquisition error
iCubio
Some parts movement failed during Connect with distributor or
0xAB
periodic testing iCubio
Some parts movement failed during
periodic testing, then cancelled the Connect with distributor or
0xAC
following operation but just to read iCubio
optoelectronic data.
Position information error happened during Connect with distributor or
0xB1
residual checking testing iCubio
0xB3 Parameter configuration failure
iCubio
0xB4 parameter query failure
iCubio
0xC1 Pure water shortage
iCubio
0xC2 waste liquor overflow
iCubio
0xC3 Reaction tray overflow
iCubio
0xD1 parameter error
iCubio
0XD2 Can't find the extension information
iCubio
0XD3 results query failure
iCubio
0xD4 Preset switch loss
iCubio
0xD5 Off switch loss
iCubio
0xD6 Command is not supported
iCubio
0xD7 Distance setting loss
iCubio
Attachment 2: The software fault code table
Trouble Fault Description Trouble shooting
112 / 120
unit code
0xF1 Samples shortage supplement samples
0xF2 Reagent shortage Supplement reagent
IU
0xF3 Abnormal reagent blank check reagent validity
0xF4 Diluents shortage Supplement diluents
0x00 Open wrong communication equipment Connect with distributor or
iCubio
0x01 Communication request is refused Connect with distributor or
iCubio
0x02 Communication frame packaging error Connect with distributor or
iCubio
0x03 Communication request timeout Connect with distributor or
iCubio
0x04 Communication result timeout Connect with distributor or
iCubio
0x05 Communication frame head error Connect with distributor or
CM
iCubio
0x06 Communication frame tail error Connect with distributor or
iCubio
0x07 Abnormal communication frame length Connect with distributor or
iCubio
0x08 Communication frame content error Connect with distributor or
iCubio
0x09 Communication frame validation error Connect with distributor or
iCubio
0x0A Other communication error Connect with distributor or
iCubio
0x00 Check reaction cup and
Abnormal cup more than threshold
IM light way
0x01 Abnormal reaction blank Cleaning reaction cup
0x01 Check whether the reagent,
Reagent, sample frame is not in place
sample frame is in place
TF
0x02 Sample shortage, skip the test Supplement sample
0x03 Reagent shortage, skip the test Supplement sample
0x0B Connect with distributor or
Temperature anomaly
iCubio
DC
0x0C Connect with distributor or
Light source anomaly
iCubio
113 / 120
Attachment 3: Trouble shooting table
Function
No. Troubles Troubleshooting
module
AC power supply is abnormal
Fuse failure
No action while power
Switcher is broken
on
Power board failure
Main board failure
Displayer failure
Windows system failure
Can’t log in windows PC main board failure
1 Power on
Hard disk failure
PC power supply is abnormal
RS232 cable is loose or connect
incorrectly
Can’t connect to Serial Port set incorrectly
analyzer Serial port of PC failure
Serial port of analyzer failure
Main board failure
Sample probe position is not directly
above the cuvettes, lead probe
scratch cuvettes wall while mixing
Reaction tray home position is not
directly beneath the sample probe,
cuvettes scratch lead probe scratch cuvettes wall
while mixing
Cuvettes offset modulation inaccurate
leads to the reaction tray stopped,
Reaction tray
2 offset is not adjusted correctly
Module
Reaction tray lost steps
Gear on the drive axis is loose
Gear is wear down
Reaction tray lost
Gears match up too tight lead step
steps
motor lost steps
Driving belt is loose
Reaction tray interval Gear is wear down

too large and sound
Gear is loose
114 / 120
abnormal Gland nut of reaction is loose
Reaction cuvette drive motor is
broken
Reaction cuvette drive circuit board
Reaction tray can’t failure
rotate normally Connecting line is loose
Reaction tray home position sensor
failure
Gear is wore down or loose
Cuvettes 1 is not
Home position adjusted incorrectly
directly beneath
sample adding
Home position sensor failure, reset
position after
failure
mechanical resetting
Waiting time is not enough from
power on
Temperature sensor failure
Temperature
Abnormal output of main board
abnormal
heating voltage
Heating belt failure
Main board failure
Heat belt failure
Temperature can’t Main board control signal error
reach 37℃ Abnormal main board heating voltage
Temperature sensor failure.
Lamp decayed
Gains adjustment is not enough
Filter wheel rotate abnormally
Filter wheel home position and
wavelength 1 position is not corrected
adjusted
Signal accept board is broken( check
Air / water blank AD
Light path if power supply normal or not, the
3 value is low
assembly silicon photodiode is normal or not)
Signal conditioning board is broken
(check if power supply normal or not,
digital adjustable resistance IC and
AD sampling IC is normal or not)
Main board is broken (Can't normally
output spi signal/U26)
Air / water blank AD Gains adjustment is too large.
115 / 120
value saturation Stray light affects too much
Main board is broken (Can't normally
output spi signal/U26)
Signal conditioning board is broken
(check if digital adjustable resistance
IC and AD sampling IC is normal or
not)
Filter wheel step motor control signal
is abnormal. (Technology direction)
Filter wheel step motor is broken
Filter wheel rotate
Filter wheel home position sensor is
abnormally.
broken (signal cannot output
normally)
Filter motor drive circuit is broken
Lamp failure or poor contact
(including lamp cable connector)
Lamp lose, skew, loose or the lamp
340nm AD is low seat is installed incorrectly.
(normal AD is Lamp seat cover screw does not tight
0.8v~1.1v) cause the lamp cover fall or shift
Lamp line connects is bad.
Signal accepter, light guide beam,
light source are not in a straight line.
Cuvettes are dirty.
Reaction tray home position sensor is
Absorbance fluctuate
adjusted incorrectly (Should be
a lot while test during
adjusted to the time when the
the rotation of reaction
cuvettes edge just pass through the
tray
light source, and the sensor signal
jumps from 1 to 0)
Probe position adjusted incorrectly
Reagent, sample, cuvettes placed
incorrectly.
X, y, z step motor lost steps or
Probe collide
blocked
Sample arm
4 Reaction tray cover placed incorrectly
Module
Reaction tray module destroyed
Liquid levels detect board failure
Home position failure
x、y、z axis step motor
Line process in poor connect (blocked
works abnormally
or lost steps)
116 / 120
Motor interface board failure
Drive board failure
Main board failure
Probe move to top
over and sound Z axis step motor home position
abnormal as failure
mechanical blocked
Probe move to right
over step home
position sensor and X axis step motor home sensor failure
sound abnormal as
mechanical blocked
Probe move back over
step home position
Y axis step motor home position
sensor and sound
failure
abnormal as
mechanical blocked
Can’t detect liquid Line process in poor connect
level Probe failue
Main board failure
There is liquid between outer layer
Liquid levels detect and inner layer of probe.
some time bad Line process in poor connect
X direction sample position incorrect
means x axis home position adjust
incorrectly
Y direction wash position incorrect
means y axis home position adjust
incorrectly
Special position move
Others special position incorrect
incorrectly
means software setting incorrect
Power supply for step motor is
abnormal
Interface board failure
Step motor drive board failure
Main board failure
Teflon pipe and the probe didn’t
Sample probe drop
connect tightly.
liquid
Probe got weld crack
117 / 120
Probe is broken
Peltier failure
Peltier install incorrectly
Temperature of Peltier power supply is abnormal
reagent rack can’t Radiating fan failure or not stable
meet requirement Radiating fan power supply is
abnormal
Power board failure
Line process in poor connect
DC mixing motor failue
Interface board failure.
Sample probe mix
Mixing circuit of motor driver board
abnormally
5 Mixing module failure
Synchronization belt is broken or got
loose
Probe collides or Eccentricity gears wear down
scratches cuvettes. Motor lost steps
Wash head position adjusted
incorrectly
Reaction tray does not rotate or rotate
Wash needle collide incorrectly
Reaction tray cover placed incorrectly
Wash needle installed incorrectly
Wash needle is out of shape.
up-and-down motion synchronization
belt is broken
Synchronization belt clamp broke
Wash arm can’t go Vertical step motor failure
Washing
6 down or up Line process in poor connect
Mechanism
Step motor power supply abnormal
Interface board failure
Main board failure.
Lack of clean water
topping-up pump is dirty, clotted, or
failed
No water inject into
Junction piece failure
cuvettes while
Water pipe is folded
washing
Pump, valve connects incorrectly
Flow path connect incorrectly
Liquid drive board failure
118 / 120
Reaction tray move incorrectly
Wash needle clean position is not low
enough to enter cuvettes
Suck pump is dirty, clotted or failed.
Clean water overflow
Suck pipe is clotted, suppressed or
while wash cuvettes
got air leakage
Pump, valve connects incorrectly
Flow path pipe connect error.
Liquid drive board failure.
Suck pump is dirty, clotted or failed
Suck pipe is clotted, suppressed or
Residual in cuvettes is
got air leakage
too much after
Probe position should be adjusted to
washing
the bottom of the cuvettes
Flow path junction piece failure
Suck valve failure
Aspirating needle drop Injection pump diaphragm is dirty
liquid Injection pump control signal error
Flow path drive board failure.
Probe clot
Injection pump is dirty, blocked or
failed.
Probe got no inside Junction piece failure.
wash water spray Pipes connect incorrectly
Ram pump valve failure
Pump or valve connects incorrectly
Flow path drive board failure.
Probe wash pump failure or is dirty
Liquid junction piece failure
Pipes folded
Outside wash water valve adjusted to
Wash cell got no water
be too small
Pump or valve connects incorrectly
Liquid pipes connect incorrectly
Flow path drive board failure
The injection probe of Pump connects incorrectly
the wash arm inject
while washing the Liquid pipes connect incorrectly
sample probe
The sample probe Pumps connect incorrectly
119 / 120
inject while washing
Liquid pipes connect incorrectly
cuvettes
Wash arm move to the
top and sound
Home position sensor failure
abnormal as
mechanical blocked
Ram pump leakage
Do not dispense Ram pump motor failure
reagent and sample Interface board failure.
Sensor failure
There are bubbles in the pump, or
Dispense volume pump leakage problem
incorrect Pulse equivalent settings incorrect
Valve failure.
Line process in poor connect
7 Ram pump
Stepper motor power supply is
Ram pump motion abnormal
abnormal Home position sensor is broken
Interface board failure.
Main board failure.
Sample probe move to
top and sound
Home position sensor failure.
abnormal as
mechanical blocked
Can’t run software by File lost or can't start the
the shortcut on corresponding program of the
Software
8 desktop. installation directory
Management
Data base initialization Data base is broken.
error. SQL server does not start
120 / 120

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iCubio Corporation has the final explanation on the service.

reliability and performance responsibility, namely:

person with qualification approved by iCubio;

2 The electrical equipment in line with national standards;

3 Products in accordance with the instruction manual for operation.

Free Services range:

Service fee range:

implement fee-based services;

natural disasters; replacement of accessories without iCubio's allow, supplies, or by non-iCubio

authorized person maintenance machine.

The company returned to iCubio, please follow these steps:

brief the reason for the return.

Service Manual release date:2015-07,Version: V.2.3

Shenzhen iCubio Biochemistry Technology Co.,Ltd

Customer service department

Guangming new district, Shenzhen, China.

24-hour hotline: 4006350600

Tel： +86 755-26610931

Fax： +86 755-26610893, 755-61658199

iMagic series is discrete auto-wash chemistry analyzer, 100-120test/hour, with reagent

2.1 System Structure：

Reagent and sample

Figure 2-1 Front view

Figure 2-2 Back interface

Deionized water sensor

Deionized water 1 Deionized water 2

Waste liquid 1 Liquid waste 2

Figure 2-3 The side of liquid road interface(with auto cleaning)

Figure 2-4 The side of liquid road interface(without auto cleaning)

2.2 Reaction Tray System

Figure 2-5 Reaction tray

Figure 2-6 Cuvettes

2.2.2 Light Source System

Figure 2-7 Light source system

2.2.3 Filter System

Figure2-8 Filter system

2.2.4 Heating System

Figure 2-9 Heating system

2.2.5 Photoelectricity Conversion System

Signal processing board

Figure2-10 Photoelectricity conversion system

2.2.6 Machinery Motion &Transfer System

Figure2-11 Machinary Motion Transfer System

2.3 Reagent System

2.3.1 Reagent Rack and Sample Rack

Reagent Rack Sample rack

Figure 2-12 Reagent and sample rack

Step motor Collide protect

Figure 2-13 Sample Probe

2.3.3 Ram Pump

2.4 Mixing System

Mixing gear Reagent rack

2.5 Wash System

Figure 2-16 Wash system

2.6 Control System

2.7 System Connection

Figure 2-17 Connection Diagram

3.1 Check before Installation

3.2 Installation Requirement

3.2.1 Space Requirement

Figure 3-1 Space requirement

3.2.2 Environment Requirement