Topic Name:

Recombinant
protein
production

Definition:
Manipulated

protein formed through

Recombinant DNA technology, in
which a large quantity of proteins are
produced through the insertion of the
desired protein gene into an
expression vector which must
contain a promoter so that the protein
is expressed.

HISTORY:
1796:

First protein vaccine produced (cow

pox vaccine) by EDWARD JENNER
1922:First protein pharmaceutical
produced (insulin) by BANTING and BEST
1970s:Recombinant biotechnology
flourished due to discovery of
recombinant DNA by BERG , COHEN and
BOYER
1990s: Many enzymes produced by
recombinant techniques

 1993s:

50% of industrial enzymes

provided by recombinant processes.
Mouse cells are used in the bio
manufacturing of recombinant proteins
(Barnes et al.2000)& also function as
feeder cells for embryonic stem cells.
2009, the number of protein-Based
recombinant pharmaceuticals lincensed
by the US food and drug administration.

IMPORTANCE:
Products

of these industries in turn augment

the fields of medicine, diagnostics, food,
nutrition, detergents, textile, leather, paper,
pulp and skin care products.
Recombinant proteins benefit major sectors of
the
-Biopharmaceutical industry
-Enzymes industry
-Agricultural industry
-Food and fermentation industry