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Nucleic acid
Ribonucleic acid, RNA
Section 1. Composition of nucleic acids
Nucleosides phosphate
Bases Sugar
purines ribose
pyrimidines deoxyribose
1. Bases
Purines and pyrimidines
Purines :
adenine , guanine
Pyrimidines :
cytosine, thymine
2. Ribose (in RNA) and deoxyribose (in DNA)
b
b
HOCH2 O OH HOCH2 O OH
OH OH OH (no O)
ribose deoxyribose
8
CH2OH CH2OH
OH 5 OH
5
4 1 b 4 1
H
H H H 3 2 H H
H 3 2 H
OH OH OH H
Ribose Deoxyribose
3. Ribonucleosides
Ribonucleoside = ribose/deoxyribose +
bases
NH2
O O N
O- P O CH2
-
O
O
OH
13
Base Sugar Nucleoside
Adenine (A) ribose Adenosine
Guanine (G) ribose Guanosine
Cytosine (C) ribose Cytidine
Uracil (U) ribose Uridine
14
4. Nucleotides
Nucleotide = nucleoside + phosphate
NH2
N
CMP
O O N
O- P O CH2
-
O
O
3
3,5-phosphodiester bond NH2
OH N
N
O 5 N N
O P O CH2
-
O AMP
O
OH 16
DNA
dAMP Deoxyadenosine monophosphate
dGMP Deoxyguanosine monophosphate
dCMP Deoxycytidine monophosphate
dTMP Deoxythymidine monophosphate
RNA
AMP adenosine monophosphate
GMP guanosine monophosphate
CMP cytidine monophosphate
UMP uridine monophosphate
17
5. Nucleoside diphosphates and
triphosphates
6. Polynucleic acid chain
James D. Watson
DNA contains two strands of nucleotides
Hydrogen (H) bonds hold the two strands
in a double-helix structure
A helix structure is like a spiral stair case
Bases are always paired as AT and GC
Thus the bases along one strand
complement the bases along the other
25
Two H bonds for A-T
Three H bonds for G-C
26
Key points on DNA double helic structure
(1) DNA is composed of two strand wound
round each other to form a double helix.
The two DNA strands are organized in an
antiparallel arrangement: the two strands
run in opposite directions, one strand is
oriented 53 and the other is oriented 3
5.
(2) The bases on the inside and the sugar-
phosphate backbones on the outside.
(3) The diameter of the double helix is 2 nm,
the distance between two base is 0.34 nm,
each turn of the helix involves 10 bases
pairs, 34 nm.
(4) The bases of two strands form hydrogen
bonds to each other, A pairs with T, G pairs
with C. this is called complementary base
pairing.
(5) stable configuration can be maintained by
hydrogen bond and base stacking force
The antiparallel
nature of the DNA
double helix
Conformational variation in double-
helical structure
B-DNA
A-DNA
Z-DNA
3. Tertiary structure : Supercoils
Supercoils: double-stranded
circular DNA form supercoils.
The DNA in a prokaryotic cell is a supercoil.
sentence gene
book DNA
Section 3. Structures and functions of RNA
1. Types :
mRNA: messenger RNA, the carrier of genetic
information from DNA to translate into protein
tRNA: transfer RNA , to transport amino acid
to ribosomes to synthesize protein
rRNA: ribosome RNA, the components of
ribosomes
hnRNA: Heterogeneous nuclear RNA
snRNA: small nuclear RNA
Ribosome
RNA molecules are largely single-stranded but
there are double-stranded regions.
The carrier of genetic information from
DNA for the synthesis of protein.
Composition: vary considerably in size
(500-6000 bases in E. coli)
Eukaryotic mRNA Structure
A component of ribosomes.
Ribosomes are cytoplasmic structures
that synthesize protein, composed of
both proteins and rRNA.
The ribosomes of prokaryotes and
eukaryotes are similar in shape and
function. The difference between them is
the size and chemical composition.
rRNA
RIBOSOM
Ribosom
Ribosom Ribosom
Mitokondria
prokariot eukariot & kloroplas
Unit 70S Unit 80S Unit 70S
Subunit 30S Subunit 50S Subunit 40S Subunit 60S Subunit 30S Subunit 50S
The organization and composition of
prokaryotic and eukaryotic ribosomes
The proposed
secondary structure
for E. coli 16S rRNA
Transfer RNA (tRNA)
Function: Transport amino acids to ribosomes for
assembly into proteins.
Primary Structure :
Average length: 75 bases
Modified bases: pseudouridine
methylguanosine
dihydrouridine
The sequence CCA at the 3 terminus
Secondary structure: warped cloverleaf
RNA Protein
DNA