Experimental Designs

Farid Hariri 95002309

Professor: Dr. Baghban Salehi
Spring 1396
Enhancement of bioleaching of a spent
Ni/Mo hydroprocessing catalyst
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Abstract
Statistically based experimental designs were applied to
screen and optimize the bioleaching of spent
hydrocracking catalyst.
Eleven factors were examined for their significance on
bioleaching using a PlackettBurman factorial design.
Four significant variables were selected for the
optimization studies.
The combined effect of these variables on metal
bioleaching was studied using a central composite
design (CCD).
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PlackettBurman factorial design

To select the significant variables for biological metal
extraction, factors were tested and identified via the
PlackettBurman design experiment. Because Mo was
the most important heavy metal present in the spent
catalyst, its recovery (%) was used as the response in
the PlackettBurman design experiment.
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PlackettBurman factorial design

S. No. Variables Unit Lower values Higher values
1 Temperature C 22 35
2 pH 5 9
3 Shaking rate Rpm 110 160
4 Particle size m 38 220
5 Pulp density % w/v 1 5
6 Inoculation size % w/v 2 10
7 Preculture time Day 4 10
8 Sucrose conc. g/L 80 120
9 NaNO3 conc. g/L 1 2.5
10 KH2PO4 conc. g/L 0.25 0.75
11 Yeast extract conc. g/L 0.5 3
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PlackettBurman factorial design

Screening:
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PlackettBurman factorial design

Screening:
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PlackettBurman factorial design

Screening:
T-value of effect

Rank
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PlackettBurman factorial design

Screening:
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PlackettBurman factorial design

The variables with higher absolute effect factors were
considered to have a greater impact on the biological
metal extraction.
Yeast extract concentration
NaNO3 concentration
Pulp density
Sucrose concentration
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Central Composite Design (CCD)

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Central Composite Design (CCD)

Step 1:
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Central Composite Design (CCD)

Step 2:
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Interactions: AB
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Interactions: AC
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Interactions: AD
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Interactions: BD
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Interactions: CD
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Molybdenum Analysis
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Molybdenum Analysis
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Molybdenum Analysis
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Molybdenum Analysis
Predicted vs. actual plot for Mo recovery (%):
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Molybdenum Analysis
Contour:
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Molybdenum Analysis
3D Surface:
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Nickel Analysis
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Nickel Analysis
Predicted vs. actual plot for Ni recovery(%):
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Nickel Analysis
Contour:
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Nickel Analysis
3D Surface:
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Aluminum Analysis
R-Squared = 0.9202
Adj R-Squared = 0.8554

0.9202 0.8554 < 2 Significant

= +12.10 0.88 0.27 + 0.25 0.84 + 0.29
+ 0.62 0.32 0.32 0.36 0.792
0.392 0.088 2
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Aluminum Analysis
Predicted vs. actual plot for Al recovery(%):
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Aluminum Analysis
Contour:
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Aluminum Analysis
3D Surface:
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Optimization
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Optimization

Constrains:
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Optimization
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Optimization

Correlation Confirmation Confidence Interval (95%)

Responses (%) Target
predicted experiment (%)
Low High

107.4
Mo recovery Maximize 98.2 98.8 0.9 87.7

52.9
Ni recovery Maximize 48.3 46.5 0.6 38.5

Al recovery Maximize 14.3 13.7 0.4 11.7 16.9

Thank you for your attention