PS-1 PRESENTATION

Phospholipid Mesoporous for drug delivery

Sindhu M
2017B1A30712P
 INTRODUCTION

• Drug delivery system

• Surface properties determine
interaction with other
molecular species
• Inclusion of proteins to modify
surface properties.

Fig1: Liposome
 INTRODUCTION

Mesoporous particles
• Material containing pores with diameters between 2 and 50 nm.

• Ordered pore structure and high specific surface area

• Applications: Adsorbant, catalysis, drug delivery, enzyme encapsulation

MPP- Mesoporous Phospholipid Particles

• Properties of mesoporous particles and liposomes

• Liposomal properties: High bio compatibility, ability to accommodate both

hydrophilic and hydrophobic guest molecules, easy surface modification

Collagen in liposomes

• To modify surface properties

• As gel matrix for drug delivery

 OBJECTIVES

1. Study the properties of collagen in non polar solvents

2. Preparation of mesoporous particles and study of its properties.

 RESULTS AND DISCUSSION

• 2 alpha bands, 2 beta bands and one

gamma band was obtained as expected,
confirming that the sample did contain type
SDS Fig2:SDS
PAGEPAGE ofofcollagen
collagen
I collagen

• Two more faint bands were also observed

near the gamma region. This could be due
to broken alpha1 or alpha2 chain.

Bands expected for type 1 collagen

Bands expected for type 1 collagen
 RESULTS AND DISCUSSION
UV-Vis Spectrum

• A significant upward shift of spectral

curves was observed as the hydrophobic

nature of solvent increased

• Solvent 3, containing the highest

percentage of t-butyl alcohol is closest to

Fig3: UV-Vis spectrum of collagen in non polar mixtures. Ratios given in figure are
t-butyl alcohol : Hexane.
control.
 RESULTS AND DISCUSSION
Spectrum of collagen in the presence of lipids

(A) (B) (C)

Fig4: UV-Vis spectrum of collagen in the presence of lipids. (A) is the spectrum in solvent 1 and in the presence of 1% and 6% Lipid.
(B) is the spectrum of collagen in solvent2 and 6% lipid. ( C) is the spectrum of collagen in solvent3 and in 1% and 6% Lipid.

• Mixture 1 shows the most variation in spectrum at around 280nm

• In mixtures 2 and 3, lesser variation is observed in the presence of lipid around

280nm
 RESULTS AND DISCUSSION
Fibrillogenesis of collagen ( Control)

• Sigmoidal curve was obtained for

fibrillogenesis of collagen in polar

solvent

• This can be attributed to two

different phases in the process of

fibrillogenesis- Lag phase and the

log phase
Fig5:Absorbance at 313nm as a function of time
Fibrillogenesis of collagen in 0.5M Acetic acid
 RESULTS AND DISCUSSION
Fibrillogenesis of collagen in non polar solvents and in the presence of lipids

Fig4:Absorbance at 313nm as a function of time

• Sigmoidal curve is observed only in solvent 3

• Sigmoidal curve is not obtained in any of the three mixtures in the presence of lipids
 RESULTS AND DISCUSSION
FTIR spectra of prepared mesoporous particles

Wavelength ( 1/cm) Wavelength ( 1/cm)

(A) (B)

Fig7: FTIR spectra of prepared mesoporous particles in 6% HSPC solutions in

(A) 1:2, (B) 1:1 and © 2:1 ratios of t-butyl alcohol and cyclohexane.

Wavelength ( 1/cm)
(C)

• 1741 1/cm : Carbonyl group

• 1260 1/cm: P=O
• 1064 1/cm: P-O-C
• Shift in P=O band: Formation of H-bonded network of HSPC head groups
 FUTURE DIRECTIONS

• Drug loading

• Cellular uptake

• Drug release

• Toxicity studies

• Comparison with other modes of drug delivery