Gram Negative

Cocci
BY: PAUL AERON E. BANSIL, RMT
Gram Negative Cocci

◦ Neisseria
◦ Moraxella

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Neisseria

◦ Obligate Aerobes
◦ Non-Motile, Non Hemolytic
◦ Microscopic: Gram Negative
diplococci or Coffee bean or Kidney
Shaped
◦ Culture: small, gray-white opaque,
convex and glistening
◦ Oxidase (_), Catalase (+)
◦ CHO Fermenters
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 It is used for the collection of
TRANSITION HEADLINE

specimen for N. gonorrhea

1 DACRON/RAYON SWABS

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Neisseria gonorrheae

◦ MOT: Sexual Contact

◦ Leading cause of STDs
◦ Common Pili
◦ Requires Iron for Growth

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Neisseria gonorrheae

◦ Gonorrhea
◦ Purulent Urethritis/Arthritis
◦ Ophthalmia Neonatorum

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Neisseria gonorrheae

◦ Transport Medium
◦ Cotton Swabs should be avoided
◦ Dacron/Rayon Swabs
◦ Candle Jars

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Laboratory Diagnosis for N.
gonorrheae
◦ Gram Staining
◦ Culture
- BAP and CAP
- Thayer Martin Agar
- Modified Thayer Martin Agar
- Martin Lewis Agar
- New York City Medium
o CHO Utilization Test
o Oxidase Test
o Superoxol Test 8
Neisseria meningitidis

◦ Meningococcal meningitis
◦ Leading cause of bacterial
meningitis
◦ Waterhouse Friderichsen Syndrome
◦ Requires iron for growth

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Non-pathogenic Neisseria

◦ N. cinerea
◦ N. flavescens
◦ N. lactamica
◦ N. sicca – Bread Crumb Like Colonies
◦ N. elongata – Rod Shaped GN cocci

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Moraxella catarrhalis

◦ 3rd most common cause of Otitis

Media in Children
◦ Only member of the family
Neisseriaceae that hydrolyze
DNAse
◦ Hockey-puck colonies
◦ Does not ferment any sugar in the
CTA medium
◦ Reduces Nitrate to Nitrite
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CHO UTILIZATION TEST

◦ Medium: Cystine Trypticase Agar

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