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  • Dark Field Microscope

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    SamShane
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    The dark field microscope uses only light scattered by the specimen to produce an image of an illuminated specimen against a dark background. It has an opaque disc that blocks straight light and a condenser lens to focus only scattered light, allowing observation of unstained live samples in liquid. Dark field microscopy provides a cheaper alternative to phase contrast microscopy and enables visualization of specimens not visible with brightness contrast alone, such as diagnosing syphilis by examining Treponema pallidum bacteria.

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    Microscope

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    The dark field microscope uses only light scattered by the specimen to produce an image of an illuminated specimen against a dark background. It has an opaque disc that blocks straight light and a condenser lens to focus only scattered light, allowing observation of unstained live samples in liquid. Dark field microscopy provides a cheaper alternative to phase contrast microscopy and enables visualization of specimens not visible with brightness contrast alone, such as diagnosing syphilis by examining Treponema pallidum bacteria.

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    9 views8 pages

    Dark Field Microscope

    Uploaded by

    SamShane
    The dark field microscope uses only light scattered by the specimen to produce an image of an illuminated specimen against a dark background. It has an opaque disc that blocks straight light and a condenser lens to focus only scattered light, allowing observation of unstained live samples in liquid. Dark field microscopy provides a cheaper alternative to phase contrast microscopy and enables visualization of specimens not visible with brightness contrast alone, such as diagnosing syphilis by examining Treponema pallidum bacteria.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 8

    Dark Field Microscope

    Description
    - A type of microscope that makes use of only light scattered by a
    specimen

    - It ignores other light not coming from the specimen

    - Thereby producing an image of an illuminated specimen in a dark


    background
    Principle
    Opaque disc & Condenser lens

    - Disc only allows outer ring of light

    - Focused by condenser lens

    Only the light scattered by specimen is collected

    - Causing an illuminated image of specimen in


    dark background
    Significance & Usage
    Usage

    - Used for live & unstained specimens

    - Used to view specimen suspended in liquid sample

    Significance

    - Cheaper alternative of phase contrast

    - Enables you to see things not visible with the help of brightness contrasts
    Other Facts
    - J.J. Lister invented the dark field microscope in 1830

    - Dark Field Microscopy is used to diagnose Syphilis by examining the


    presence of Treponema pallidum
    Sources
    http://www.ruf.rice.edu/~bioslabs/methods/microscopy/dfield.html

    https://www.cdc.gov/std/syphilis/manual-1998/chapt5.pdf

    http://www.mlo-online.com/darkfield-microscopy-for-point-of-care-syphilis-diagnosi
    s.php

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