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Introduction - Basic Principle of GC - Schematic Diagram of GC - Components of GC - Advantages & Disadvantages - Applications
Introduction - Basic Principle of GC - Schematic Diagram of GC - Components of GC - Advantages & Disadvantages - Applications
• Introduction
• Basic Principle Of GC
• Schematic Diagram of GC
• Components of GC
• Advantages & Disadvantages
• Applications
Introduction
Gas chromatography (GC)
•Analytical Technique
• Developed by Martin and Synge in 1941
• They suggested the use of gas-liquid partition chromatograms for
analytical purposes.
• It is a process of separating component(s) from the given crude
by using a gaseous mobile phase.
• Two major types:
• Gas-solid chromatography
(stationary phase: solid)
• Gas-liquid chromatography
(stationary phase: immobilized liquid)
Basic Principle of GC
• It involves a sample being vaporized and injected onto the
head of the chromatographic column. The sample is
transported through the column by the flow of inert, gaseous
mobile phase. The column itself contains a liquid stationary
phase which is adsorbed onto the surface of an inert solid.
Schematic diagram of a gas chromatograph
Components of Gas chromatography
• Carrier gas
- He (common), N2, H2, Argon
• Sample injection port
- micro syringe
• Columns
2-50 m coiled stainless steel/glass/Teflon
• Detectors
-Flame ionization (FID)
-Thermal conductivity (TCD)
-Electron capture (ECD)
-Nitrogen-phosphorus
-Flame photometric (FPD)
-Photo-ionization (PID)
Carrier gas
Advantages:
• The technique has strong separation power and even complex mixture can be
resolved into constituents
• The sensitivity of the method is quite high
• It gives good precision and accuracy
• The analysis is completed in a short time
• The cost of instrument is relatively low and its life is generally long
• The technique is relatively suitable for routine analysis
Disadvantages:
• Limited to volatile samples
• Not suitable for samples that degrade at elevated temperatures (thermally labile)
• Not suited to preparative chromatography
Applications of Gas Chromatography
• Separation and identification of volatile materials, plastics,
natural and synthetic polymers, paints, and microbiological
samples
• Miscellaneous-analysis of foods like carbohydrates, proteins,
lipids, vitamins, steroids, drug and pesticides residues, trace
elements
• Pollutants like formaldehyde, carbon monoxide, benzene, DDT
• Inorganic compound analysis
• Dairy product analysis- rancidity