This document provides guidance on how to effectively read and understand a scientific research paper. It outlines the typical structure of a paper, including the title, authors, abstract, introduction, methods, results, discussion, and references sections. It explains what kind of information is typically included in each section and offers tips for reading, such as taking notes on the research question, hypothesis, conclusions, and significance. The goal is to help readers comprehend and critically evaluate the paper.
This document provides guidance on how to effectively read and understand a scientific research paper. It outlines the typical structure of a paper, including the title, authors, abstract, introduction, methods, results, discussion, and references sections. It explains what kind of information is typically included in each section and offers tips for reading, such as taking notes on the research question, hypothesis, conclusions, and significance. The goal is to help readers comprehend and critically evaluate the paper.
This document provides guidance on how to effectively read and understand a scientific research paper. It outlines the typical structure of a paper, including the title, authors, abstract, introduction, methods, results, discussion, and references sections. It explains what kind of information is typically included in each section and offers tips for reading, such as taking notes on the research question, hypothesis, conclusions, and significance. The goal is to help readers comprehend and critically evaluate the paper.
Modified from Kelly Hogan’s “How to read a scientific paper”
Typical anatomy of a paper • Title & authors • Abstract (or summary) • Introduction • Methods (or Materials & Methods) • Results (including figures/tables) • Discussion (or Conclusions) • Acknowledgements • References Title • Very descriptive • May state the main finding right in the title • Is not about being creative and “catchy” (usually)
Examples The evolution of social monogamy in mammals.
mTORC1 phosphorylation sites encode their
sensitivity to starvation and rapamycin. Authors • Order of authors is important • What can you tell from the order? • You can often associate the work with the lab (the PI’s lab)
Example The evolution of social monogamy in mammals.
D. Lukas and T. H. Clutton-Brock
Abstract • Purpose of the study • Major findings • Relationship between findings & the field overall • Gives you the highlights of the whole paper Introduction • Presents background information • Sets the stage so the reader can see why the work described in this paper is significant • Written for a fellow scientist (but possibly a scientist in another field – needs to be understandable) Introduction • Structure is usually: – Accepted state of knowledge in the field – Focus on a particular aspect of the field, sometimes on the set(s) of data that lead directly to the work of this paper – Statement of research problem/question & hypothesis – Conclusions (authors will sometimes put a spoiler at the end of the introduction) Reading the Introduction • If you’re using a paper for a research project, you should jot these things down as you take notes: – What knowledge gap or data set led to the work of this paper? – What is the research question/problem? – What is the hypothesis being tested? – What are the basic conclusions stated at the end of the introduction? Methods (or Materials & Methods) • Should be detailed enough for someone else to replicate the work (volumes, concentrations, times, etc.) • Author assumes the reader is scientifically literate and understands the basics (e.g., how gel electrophoresis works) • In reality, methods are often compressed and a citation may be included. You may need to look up the cited paper for more detail. Should you read the methods at all?
• For your first assignment in this class…Yes!
• When doing a literature search, you can often just skim the methods • When you’re reading the results, you will likely have to flip back to the methods a few times to clarify how the experiment was done Results • Describes the outcome of the experiments that were done to answer the research question posed in the introduction • Results often simply stated without any interpretation (interpretation comes in the Discussion) • Figures & tables show the reader the outcomes of the experiments Reading the Results • Read the through the text, and examine the figures as they are referred to within the text • With each experiment/figure you should take note of: – The basic procedure – The question they were trying to answer – The results – The conclusion you can draw from that experiment/figure – Criticisms • You might need to look up methods you’re not familiar with to fully understand the results (Wikipedia is handy for this) Discussion • Data is analyzed/interpreted. The authors explain to the reader what they think the data show (You don’t have to agree with their interpretations!) • Findings are related to other findings in the field. These findings might: – Contribute to knowledge in the field – Correct errors about the topic – Etc. • The authors tell the reader how/why the work is significant Reading the Discussion • When you’re taking notes, try to answer these questions: – What conclusions do the authors draw? – Make note of what is fact and what is their opinion/interpretation – Describe why these data are significant to the field Overall Reflection & Criticism • You read the whole paper! Hang on, don’t put it down yet. Spend a little time on the big picture. • Do you agree with the authors’ rationale for setting up the experiments the way they did? • Is it clear that their experiments support the major finding they are claiming? • Do you see patterns/trends in their data that are problems that weren’t mentioned? • Do you agree with their conclusions? Are they over- generalized or too grand? • What further questions do you have? What would you suggest they do next? Tips for success when reading papers • Spend time with each paper initially. If you are using this paper to research/write something for a class, your time investment now will pay off later. – Look up details/methods you’re unsure of – Summarize things for yourself by taking good notes – Imagine you have to teach this paper to a classmate. This will help you make sure you understand it. References • Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, Turk BE, Yaffe MB, Sabatini DM. 2013. mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Science 341: 364. • Lukas D, Clutton-Brock TH. 2013. The evolution of social monogamy in mammals. Science 341: 526-530.