BS120 Service Traininig - 01.01.A

BS-120 Service Training
International Service Dept.

Mindray Co.,Ltd.
Agenda
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software upgrade
Maintenance
Work Flow
Yes
claim a assistance from
Mindray and inform
Find a Troubleshooting Got a S/N and details of •Find out causation
problem solution? problem
No
Yes
Need to change
Yes parts?
Under
No
Yes warranty? No
Have spare Adjust and repair it
parts? Contact salesman to
No
purchase the new parts
Repair or replace And ask for RMA
defective parts from Mindray service
department
No Get new parts
Test
Return defective parts
OK? Yes and inform the
Repair or replace
And ask for RMA from tracking number defective parts
Mindray service department
No
Return defective parts
Test
and inform the
OK?
tracking number
Get new parts

Yes
End
•Work flow
MACHINE STRUCTURE
•Machine structure
•Hardware system
(MECHANISM)
•Liquid system
•Optical system Structure

•Test results Work Principle
•Software Troubleshooting
•Maintenance
Basic Configuration
The Biochemistry analyzer consist
s of the analyzing unit (Analyzer),
Work flow operation unit (PC) and output un
it (Printer).
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
The analyzer and the computer are
connected by the cable of serial port.
(R232)
Structure
BS-120 Front View
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Structure
BS-120 Side View
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Question
Work flow
Machine structure
Hardware system
Liquid system
Optical system
How to install a new analyzer?
Test results
Software
Maintenance
Installation of
BS120
Structure
Reagent and sample tray of BS-120
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance 36 position

1~8 sample position
9~36 reagent position
Structure
Mixer and Syringe of BS-120
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Mixer Sample & Reagent Syringe

Structure
Pump & Valve of BS-120
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
•Interior Wash Pump
•Exterior Wash Pump
•EM Valve
Basic assembly
 Reagent/sample probe drive assembly

Work flow
Machine structure  Mixing bar drive assembly

Hardware system
 Reagent and Sample tray assembly
Liquid system
Optical system  Reaction tray assembly


Test results
Software
Light Source Assembly
Maintenance  ISE module Assembly
Structure
Mechanical Structure of BS-120

Sample and Reagent Probe Drive
ISE Assembly
Assembly Mixing bar drive assembly
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Reagent and Sample Tray Assembly Reaction Tray Assembly

Structure
Probe Drive Assembly
Work flow
① Movement is driven by two step
Machine structure
motors
Hardware system
② Signals are feed back by two home
Liquid system
position sensors
Optical system
Test results
③ LLD function
Software ④ Collision detection
Maintenance ⑤ Reagent preheating
Structure
Mixing Bar Drive Assembly
Work flow
Machine structure
① Movement is driven by one motor
Hardware system
② Only one home position sensor
Liquid system
③ No LLD function
Optical system
④ No Collision detection
Test results
⑤ No Reagent preheating
Software
Maintenance
Question
Probe and mixing bar drive assembly
Work flow
Machine structure
Hardware system
How to adjust the position of the mixing bar and
Liquid system
probe?
Optical system
Test results
Software
Maintenance
---------By DEBUG software
Let’s try to adjust the position!

Structure
Reagent/Sample probe drive assembly
Work flow
Machine structure
Hardware system
Installation of
Preheater
Liquid system
Optical system
Test results Arm Base Assembly Reagent pre-heater assembly
Software
Maintenance 4~15 ℃ around 35℃

Reagent Reagent bottle Reagent Reaction Cuvette in
in reagent tray pre-heater reaction tray (37 ℃)
Troubleshooting
Problems of Reagent pre-heater
Symptom Probable Cause

Work flow Corrective Action
Machine structure
Hardware system
① The 24V from Power driv
Liquid system e board is abnormal ① Check the 24V power
② The temperature control p supply
Optical system arameters are improper ② configure the parameters
The temperature could ③ The heater is defective (2. ③ Replace the heater
Test results
not reach 45 ℃ and 46~2.85K Ohm) ，
keep stable ④ Replace temperature
Software ④ The temperature sensor is
sensor
（ 1100 ~1190 Ohm ） def
Maintenance ective
⑤ The cable is not well con ⑤ Reconnect the cable
nected
Structure
Reagent tray & Refrigeration
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Structure
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Structure
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Peltier
Troubleshooting
Problems of Refrigeration
Symptom Probable Cause Corrective Action

Work flow
Machine structure
Hardware system ① The cable is not well ① Reconnect the cable

connected
Liquid system ② Replace 12V/5V board
② The 12V/5V board is
defective ③ Replace Reagent
Optical system The reagent tray could refrigeration board
③ The Reagent refrigeration
not be cooled. ④ Replace the Peltier
Test results board is defective
④ The Peltier is defective ⑤ Replace temperature
Software sensor
Maintenance ⑤ The temperature sensor is
defective
Structure
Reaction tray assembly
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
•Offer positions for reaction cuvette
•Provide proper temperature for reaction
•Provide positions for photoelectric measurement
Structure
Step motor
Step sensor
Work flow
Machine structure
Hardware system
Liquid system
Optical system Home position

sensor
Test results
Software
Maintenance
• Rotary movement is driven by one Step Motor
• Signals are feed back by Home Position Sensor and Step Sensor
Structure
Reaction Segment
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance Base for the Reaction Segment

Structure

Fans with feed back signal
Work flow Temp Sensor

Machine structure
Hardware system
Liquid system
Optical system Photodiode Board

Test results
Software
Maintenance
Overheat Protector
Troubleshooting
Temperature problems in Reaction tray
issues Probable Cause Corrective Action

Work flow
① The 220V/110 input is ab
Machine structure
normal
Hardware system ② The ambient temperature
is over 37 ℃ ① Check the power supply
Liquid system ③ The temperature control p ② Install air condition.
Optical system
arameters are improper ③ configure the parameters
The temperature ④ The heater is defective (T ④ Replace the heater
Test results op heater:437.8~483.8 Ohm
could not be stable at ⑤ Replace temperature
， Bottom heater:131.4~145.
37 degree. 2 Ohm ) sensor
Software
⑤ The temperature sensor is ⑥ Reconnect the cable
Maintenance （ 1100 ~1190 Ohm ） def
ective
⑥ The cable is not well con
nected
Troubleshooting
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Troubleshooting
Replacing the Lamp….

Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Troubleshooting
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Work principle
HARDWARE SYSTEM
Work flow
Machine structure
Hardware system
Liquid system
(Printed Circuit Boards)
Optical system
Test results Connection,

Software Function,
Maintenance Typical troubleshooting.
Work principle
Hardware Frame
NOTE:
Red: PCB Boards;
Blue: Field Components; PC Fans Peltiers
Black: Outside Parts.
Work flow
Machine structure Refrigeration

Position Sensors ISE Power
Board Board
Hardware system
Sensors in Tanks
AC Power Supply
Reaction Disk
Liquid system Temp Transducers Temperature
Main Power Supply Boards
(110/220V)
Sampling Board
Optical system
Probes LLD Boards
Board PFC Board
12V&5V Board
24V Board
Test results
Photo-electric
A/D Board
Boards
Software
Reac. Tray
Lamp
Heaters
Maintenance
Power Drive Board
Step Reagent
Pumps Valves DC Motor
Motors Preheater
Work principle
AD Conversion Board Liquid level sensor
Position Sensors
FPGA Download Interface
LLD Interface
Power supply
Power
Feedback signal of Drive
Fans Board
Reaction Temp
Sampling Board
Serial Port ISE Module

Work principle
Main Board (CPU Board)
Work flow Functions:

Machine structure  Control center of chemistry analyzer.
Hardware system  Multi-CPU processor system.
Liquid system
 Temperature control loop.
Optical system
 Power drive board control.
Test results
Software
 A/D conversion board control.
Maintenance  Signal collection of sensors.
Work principle
Jumper on Main Board
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Disable Modification Enable Modification
Software
Maintenance
Change the jumper’s position to enable the modification.
Work principle
Power Drive Board (PDB)
Functions:
Work flow
 Power drive components.
Machine structure
Hardware system
 Receive control signals for pumps, valves,
Liquid system motors and temperature switch from
Optical system main board.
Test results  Drive pumps, valves, motors and temperature
Software
Maintenance switch directly according to the signals.
Motor of Filter Wheel
Power Drive Board of BS120

Motor of Syringe
Motor of Reaction
Compartment
H-Motor of Probe
FPGA
Motor of Download Interface
Reagent
Compartment
Reagent
Motor of Pre-heater
Mixer Main Board
V-Motor of Probe Power

Control Signal of Lamp Mix Motor Valves & Pumps
Supply
Work principle
Reaction-Temperature Sampling Board
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
 Collect the Temp signal.
Maintenance
 Convert analog signal to digital signal.
 Transmit the signal to main board.
Work principle
A/D Conversion Board
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
 Filter, amplify and adjust the gains of
Maintenance
different channels of photo-electric signal.
 Convert analog signal to digital signal.
 Multi-selective gating system.
Work principle
Photoelectric Conversion Board
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Photodiode
Test results
Software Also called pre-amplification board.

Maintenance Convert optical signal to electric signal.
Amplify the electric signal.
Work principle
Reagent Refrigeration Board
Work flow J71~74 Fans for J89 Peltiers

Reaction Disk
Machine structure
J15 Fogbroom /
Hardware system J77 Lamp Fan Temp Protective Switch
Liquid system
J75 Drive
Optical system Board Fan J88 Reagent Tray
Temp Sensor
Test results
J87 Main Board
Software J84,85 Refrigeration
Fans
Maintenance
J86 12V/5V Board

12VFan 12V 12V
GNDFan GND GND
Work principle
Work flow Functions:

Machine structure  Provide Power of the two peltiers.
Hardware system
 Loop control of reagent refrigeration.
Liquid system
 Supply power to fans
Optical system
 Fans feedback from reaction disk.
Test results
Software
Maintenance
Work principle

Description
Meaning Marker Color
On Off
12V goes
Work flow 12VLED Green 12V works normally
wrong
Power supply
12VFAN works 12VFAN goes
Machine structure 12VFAN_LED Green
normally wrong
Hardware system Peltiers work Peltiers go
Peltiers PELTIER_LED Green
normally wrong
Liquid system FAN1_LED Red
Corresponding
Temperature FAN2_LED Red Corresponding fan
Optical system fan works
control fans FAN3_LED Red goes wrong
normally
Test results FAN4_LED Red
Refrigeration Refrigeration
Software GREEN_LED Green temperature is temperature is
within 0-15℃ beyond 0-15℃
Maintenance
Refrigeration
YELLOW_LED Yellow temperature is temperature is
temperature
below 0℃ above 0℃
RED_LED Red temperature is temperature is
above 15℃ below 15℃
Work principle
Liquid Level Detection Board
VR1 Adjust Point

J66 Reagent /
Work flow LED
Anti-Collision
Sample Probe
LLD Signal
Machine structure
GND
12V
Hardware system
Liquid system
Optical system
Test results
Software
Functions:
Maintenance
 Detect liquid level (reagent probe, sample probe).
 Supply power of pre-heating module.
 Anti-collision function.
Work principle
Liquid Level Detection Board

Caution: The voltage between TP2 and TP4 should be around
4.5V, otherwise, adjust VR1 to match it.
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software TP2 Voltage TP6 Anti-Collision

TP4 GND Signal
Testing Point
Maintenance
Work principle
Power supply assembly

110/220V Top heater
Switch Board &Bottom heater
AC
Work flow
AC input 110V/220V
Machine structure
Hardware system
24V
Liquid system
Optical system
24V board
Test results APFC
Software
Maintenance C12V,5V
LAMP A12V B12V

ISE Power Board
PFC board 12V/5V board
Work principle
Power supply assembly
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
PFC Board
24V Board
12V/5V Board
Work principle
PFC Board
P3 Analyzer power switch

Work flow
Machine structure
P1 AC input
Hardware system
Liquid system
P4
Optical system 390V output P6 Lamp control signal
Test results P2 VDD P5 Output for lamp
Software
Maintenance • EMI filter network

• AC/DC conversion, supplying the +390V and VDD voltage to 24V board and
12V&5V board;
• Supplying stable 12V(4.5A) voltage for the lamp;
• Control the C12V, 5V and 24V outputs through the analyzing unit switch.
Trouble Shooting
Failures caused by PFC board
• Fans do not work.

Work flow
Machine structure • No click sound in the Power Supply

Hardware system when switching on the main switch.
Liquid system • Indicator of analyzer unit switch is off
Optical system when switching on.
Test results
• No power input on Main Board and
Software
Power Drive Board.
Maintenance
Work principle
24V Board
P404 AC input
Work flow
Machine structure P406 Heater power

output
Hardware system
Liquid system
P6 heater control
Optical system
P4 390V input
P5 24V output
Test results
P2 VDD input
Software
Maintenance
Transferring AC to the heater and controlling the heater

Converting the 390V to 24V
Trouble Shooting
Failures caused by 24V board
Power is switching on, fans are working

Work flow
and cable communication are normal.
Machine structure
 Error messages such as XXX can not
Hardware system
reach its initial position, while instrument
Liquid system
self-checking.
Optical system
 All motors/pumps/valves do not work.
Test results
Software
Maintenance
Work principle
12V& 5V Board
P353 B12V /C12V

Work flow output
Machine structure P406 C12V/5V output

(PDB)
Hardware system
P352 C12V/5V output
Liquid system (Main board)
P351 power fan
Optical system
P301 390V input
Test results
P2 VDD &SW
Software
Maintenance
Convert the 390VDC to B12V(10A), C12V(4.7A) and 5V

Trouble Shooting
Failures caused by 12V&5V Board
•Case 1
Work flow • Works of fans are failed
Machine structure • Work of refrigeration is failed
Hardware system • Work of pumps and valves is failed
Liquid system • Mechanic reset is ok
Optical system • Indicator of analyzer unit switch is normal
Test results
•Case 2
Software
Maintenance
• Works of fans are normal
• Work of cable communication with PC is failed
Trouble Shooting
ISE Power Supply Board
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Convert 12V to 24V & Provide the 24V to the ISE Assembly
Work Principle
Work flow
Machine structure
Hardware system
Liquid System
Liquid system
Introduction
Optical system
Principle
Test results
Typical troubleshooting.
Software
Maintenance
Work Principle
LLD (Liquid Level Detection ) Principle
Probe tip touches

liquid level
Work flow
Machine structure
Capacitance between
Hardware system inner and outer probe
tube is changed
Liquid system
Optical system The change is

converted to
Test results
voltage change
Software
Maintenance Voltage signal is

transmitted to main
board
Work Principle
Syringe ,Pumps and valves
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
1.Sample volume/precision 1.Reagent&Sample valve

3-45μl / 0.5μl 2.Interior wash pump
2.Reagent volume/precision 3.Exterior wash pump
30-450μL/ 1μL
Work Principle
Liquid system flow
Syringe
Work flow Probe wash well Mixing bar
wash well Probe
Machine structure Reagent valve
Hardware system
Reagent/sample
Liquid system Interior wash compartment
pump Exterior wash
Optical system pump
Test results
Software
Maintenance
Deionized water Waste water

tank tank
Work Principle
Liquid system flow
Syringe
Work flow Probe Mixing bar
wash well
Machine structure
Reagent valve
Hardware system
Reagent/sample
Liquid system
compartment
Interior wash Exterior wash Probe wash well
Optical system pump pump
Test results
Software
Maintenance
Deionized water Waste water

tank tank
Troubleshooting
LLD (Liquid Level Detection ) failures
 LLD error in reagent/sample disk when

Work flow
aspirating.
Machine structure
 LLD error in reaction disk when dispensing.
Hardware system
Liquid system
 LLD error of the reagent/sample probe in
Optical system
wash well.
Test results
Software
Maintenance
Troubleshooting
LLD failures in reagent /sample tray
Failure Probable Cause Solution

① LLD board is faulty ① Replace the LLD board
Work flow
② LLD voltage(TP2) is too ② Adjust the LLD voltage to about
Machine structure high or too low 4.5 volts
The detection
③ No liquid or liquid ③ Add reagent or sample into the
Hardware system indicator (LED) is
volume is too little bottle or tube
off or always on.
Liquid system ④ Probe is broken ④ Replace the probe
⑤ 12V of LLD is not ⑤ Check the 12V on main board
Optical system available or 12V/5V power supply board.
Test results
① The cable between LLD ① Measure the signal on LLD
Software The detection and Main board is ill board, Probe connection board
indicator is normal connected and CPU board, and reconnect
Maintenance but the probe is the cable.
failed to aspirate
② Main board is faulty.
and dispense
② Replace Main board.
Troubleshooting
LLD error in reaction tray

Work flow
Machine structure
Hardware system ① Probe detect level by • Check probe , connection

mistake so that probe
cable ,main board and LLD
Liquid system aspirate air
board
No liquid surface ② There are bubbles in
Optical system reagent bottle • Remove the bubbles
detected when
Test results sample probe ③ Reagent leakage • Syringe is not fixed well or
dispensing replace the piston of reagent
Software ④ The sample adding syringe and EM valve
position and reaction
Maintenance vertical limit position need • Adjust the corresponding
to be adjusted. parameters or upgrade
software
Troubleshooting
LLD error in wash well

Work flow No water in wash well
① The deionized water tank is not ① Click “Probe stop” and add
Machine structure enough or empty deionized water
Hardware system ② The exterior wash pump is ② Replace exterior wash pump
defective
③ Check and replace the
Liquid system ③ The tubing or the single-way blocked parts
No liquid surface valve or the 3-way connector is
Optical system detected when ④ Connect the tubing
blocked;
washing probe ④ The tubing is disconnected
Test results
Software
Maintenance
Water available in wash well
The same as LLD on reagent tray
① LLD function is abnormal
Work Principle
Work flow
Machine structure
Hardware system
Optical System
Liquid system
Optical system
Principle
Test results Typical troubleshooting
Software
Maintenance
Work Principle
Optical system
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
 8 Wavelength of Filters: 340nm, 405nm, 450nm, 510nm,
546nm, 578nm 、 630nm, 670nm.
 Lamp: 12V, 20W
Troubleshooting
Lamp Background
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Troubleshooting
Adjustment of Optical System
Work flow Procedure of Optical System Adjustment:

Machine structure
Hardware system 1. Photometric Position of Reaction Disk

Liquid system 2. Filter Offset
Optical system 3. Photoelectric Gain
Test results 4. Confirm Light Background and Dark Current
Software
Maintenance
Troubleshooting
Photometric Position of Reaction Disk

1. Turn off analyzing unit
Work flow 2. Connect two probes of oscillograph to AD start signal R
Machine structure C and the analog signal V3 respectively, both earth term
inals connected to GND.
Hardware system
Liquid system 3. Turn on the main power and the analyzing power.
Optical system 4. Enter Debug software, select Setup button on the up-rig
Test results ht of the window and input password of bs120
Software
5. Place 8 new segments to the reaction compartment.
Maintenance
6. Select the Command-Main Unit-Ordinary Rotate & M
easure Instruction, and then select Run. At the same ti
me, press Start on the oscillograph and collect signals.
7. Snap the waves
Connect two probes of oscillograph to AD start signal RC and

the analog signal V3 respectively, both earth terminals connected to GND.
Troubleshooting
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
AD start signal should be in the
Maintenance
middle of the photometric analog
signal.
Troubleshooting

AD start signal is not in the
middle of photometric analog
Work flow signal
Machine structure
Hardware system Move coder sensor of reaction

disk to adjust the start signal
Liquid system
Optical system
Test results AD start signal to the AD start signal to the

left of the Middle right of the Middle
Software
Maintenance
move the sensor along move the sensor

clockwise counter-clockwise
Troubleshooting
Filter Offset
1. Enter debug software

Work flow
2. Select Photoelectric-Filter Offset, and then click Star
Machine structure t
Hardware system 3. Wait for 3-4 minutes till the process finished
4. Click ok, then a pink line will be at the center of the firs
Liquid system
t peak, calculated by the software itself
Optical system 5. the offset of the filter equals to the value in the text box
Test results minus 100
6. Confiugure the filter offset in PARA and Speed-Reacti
Software
on Unit.
Maintenance
Troubleshooting
Filter Offset
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Troubleshooting
Photoelectric Gain Adjustment

1. Enter Debug software, select Photoelectric-Others-Test Gain, then Start.
2. After finished, gain value will performed in the Gain column. The order is
Work flow
340nm, 405nm, 450nm, 510nm, 546nm, 578nm, 630nm, 670nm.
Machine structure
3. During the test, the software will configure the gain value to the reaction unit.
Hardware system
4. After the test, select PARA and Speed-Reaction Unit-Query to confirm the
Liquid system configuration.
The order on the reaction unit is 670nm, 630nm, 578nm, 546nm, 510nm,
Optical system
450nm, 405nm, 340nm.
Test results
5. Run background test to confirm the gain configuration. The recommended
Software range for background is 48000-62000.
Maintenance 6. If the gain values of a channel are not reasonable, adjust them manually.
Change the gain value of the specific channel on PARA and Speed-Reaction
Unit. You will get low background value by configuring large gain value.
Similarly, you will get high background value by configuring low gain value.
The input range for gain value is 1-255.
Troubleshooting
Photoelectric Gain
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Note:
1. Remove the Jumper to VDD in main board;
2. Enable modifying parameters in Debug software is necessary
Troubleshooting
Optical system parameters

• Light intensity
Light intensity >70% 60%~70% <60%
Work flow
Status Normal Caution of changing Test prohibition
Machine structure
lamp
Hardware system
• Dark current
Liquid system
Optical system Normal value 0~200

Test results
Software
• Background
Maintenance Backgrounds 60,000~65,000 50,000~65,000 43,000~50,000 <43,000
Status Initial value Normal value Need to be Need to change

increased lamp
Troubleshooting
Issues of Light Intensity

Work flow
① Clean the Filter
Machine structure ① Dirty Filter ② Test gain in debug
② Abnormal gain ③ Replace pre-
Hardware system parameters
Only certain background is amplification assembly
Liquid system
very low ③ Abnormal optical path ④ Replace A/D
④ Defective A/D conversion board
Optical system conversion board
Test results
Software ① Replace the lamp

① Lamp aged
② Adjust the collecting
Maintenance ② Photoelectric signal position
collecting position is
improper ③ Check the filter check
All backgrounds low
③ Filter offset not correct ④ Replace the defective
④ Defective Pre-amplification boards
or A/D conversion board
Troubleshooting
Abnormal dark current

Work flow ① The reaction tray is not
covered
Machine structure • Cover the reaction tray
② The cable between Main
• Reconnect the cable
Hardware system board and A/D conversion
The value of dark board is not connected. • Replace Pre-amplification
Liquid system current is more than assembly
③ Defective Pre-amplification
200 • Replace A/D conversion
assembly
Optical system
④ Defective A/D conversion board
Test results board • Eliminate all Interference
⑤ Interference from outside source
Software
Maintenance
Troubleshooting
The status of reaction cuvette is unknown

Work flow
Machine structure
Hardware system
Liquid system
Optical system ① The background is too

The status of ① Reduce the backgrounds
high (65535)
reaction cuvette is through changing the gains
Test results ② Photoelectric signal
unknown ② Adjust start position of
collecting position is not
Software photoelectric collection
proper
Maintenance
Work Principle
Work flow
Machine structure
Hardware system
Test Results
Liquid system
Optical system
Test results
Software
Troubleshooting
Maintenance
Troubleshooting
Reaction Curve
Absorbance
F
•Adding R1 ： Pont A
Work flow •Preheating R1 ： Process B
E •Adding sample ： Point C
Machine structure
C •Incubating ： Process D
Hardware system •Adding R2 ： Point E
A •Reaction ： Process F
Liquid system B
Optical system D
Measuring cycle
Test results
Software
• The vertical axis indicates the absorbance
Maintenance Real value = curve value /10000
• The horizontal axis indicates the measuring cycle ： 12s
Troubleshooting
Point A: Reagent 1 is added
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
• In the first cycle, R1 is added into the reaction cuvette, so the
absorbance value here is the reagent blank of R1.
Maintenance
•Absorbance values at Point A vary with different reagents.
If the absorbance is much smaller than the value specified
in the reagent instruction, the reagent may has expired
Troubleshooting
Phase B: Preheating time
Usually, absorbance does

Work flow
not change when the
Machine structure reagent is heated
Hardware system
Liquid system
Optical system
Test results
Some reagents may have a
Software
increase/decrease of about 200
Maintenance (0.02A) in this process, but
normal, fluctuation of absorbance
between two consecutive cycles is
no more than 20.
Troubleshooting
Phase B: possible problems
Symptom Probable Cause Correction

Work flow
Absorbance fluctuates in some There are Bubbles or Remove bubbles or
Machine structure
Curves, but not all curves. contamination in cuvettes check the cuvette
Hardware system
Liquid system The collecting time matches t Adjust the parameter

he photoelectric conversion no of “Start position of
Optical system
t well photoelectric
collection” by Debug
Test results Absorbance fluctuates in almost software
all Curves
Software
Maintenance The lamp is aged Change lamp

Troubleshooting
Phase C: Sample is added
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Point C
Test results
Software
Maintenance
• Obvious change in absorbance showing that sample is added into the

reaction cuvette.
• Adding distilled water is different, it’s may cause 200 decrease.
Troubleshooting
Phase C: possible problems

Check the DC motor of
Work flow Mixing bar does not work
Mixing bar
Machine structure Adjust the parameter of

Mixing bar is over high “Mixing bar V-position for
mixing”
Hardware system
Little change The sample syringe is not properly tightened Mount the syringe again
Liquid system
Check the probe and liquid
Liquid dropping from sample probe
system
Optical system
Bubbles in sample tube Remove the bubble
Test results
Fluid tubing leakage Change the tubing
Software
Bubbles in reagent bottle Remove the bubbles
Maintenance
No change
Check the LLD
Liquid level detection of sample probe is failed
function
Troubleshooting
Phase D: Incubation
Work flow Phase D
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance • Incubation reaction at 37℃ circumstance

• The reaction curve in this phase is supposed to be smooth or
changing smoothly. The fluctuation should not be greater than 50 in
consecutive sampling cycle
Troubleshooting
Phase D: possible problems

Work flow
Absorbance fluctuates in some There are Bubbles or Remove bubbles or
Machine structure
Curves, but not all curves. contamination in cuvettes check the cuvette
Hardware system
The sampling time matches t Adjust the parameter of
Liquid system “Start position of
he photoelectric conversion n
ot well photoelectric
Optical system
collection” by Debug
Test results software
Absorbance fluctuates in almost
Software Adjust the position of
all Curves The mixing bar strikes Cuvette
mixing bar
Maintenance
The lamp is aged Change lamp

Troubleshooting
Phase E: Adding sample
Work flow
Machine structure
Hardware system Point E

Liquid system
Optical system
Test results
Software
Maintenance
• Absorbency is theoretically the summation of double reagent blank and
sample blank
• Similar situation as point A and point C.
• Considered as the starting time of the reaction in the software.
Troubleshooting
Phase E: Possible problems
Work flow
Machine structure
Check the dispensing system /
No change or little change Failure in adding R2 / Bubbles
Hardware system remove bubbles
Liquid system
Optical system
Too much Change R2 is expired or contaminated Change R2
Test results
Software
Maintenance
Troubleshooting
Phase F: Reaction process
Work flow
Machine structure
increase decrease
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance • The trend of reaction change is decided by the measure method.

• It’s the base of absorbency measurement and concentration
calculation.
• Normally it should be smooth, with no obvious fluctuation.
Troubleshooting
Phase F: Possible problems

Work flow R2 is expired or not properly Check R2 or dispensing
The degree of reaction is added system
Machine structure
not enough
The mixing bar is over high or
Hardware system
Check the mixing bar
does not work
Liquid system
Remove bubbles ， test it a
Bubbles in mixture
Optical system gain
Test results
Fluctuation is greater Change R2
Contamination in R2
Software than 100 in each cycle
Maintenance
The mixing bar Strikes the cuvette Adjust the position of
mixing bar
Troubleshooting
Different reaction type has different curve shape.

So a nearly invisible fluctuation here may impact
Work flow test results much.
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Troubleshooting
Summarization
 Analyzing the reaction curve sectionally and find the rea

Work flow
son of problem
Machine structure
 Comparing the current questionable Curves with former
Hardware system
good ones and find difference
Liquid system
 Repeating the test is very important. It can help you find
Optical system
the difference among the curves of the same test.
Test results
Software
Maintenance
Troubleshooting
Abnormal results analysis

All results are abnormal
Work flow All results are low
Machine structure Some are high, and some are low, resulting in bad
Hardware system repeatability
Liquid system 
Certain results are abnormal
Optical system Certain applications, e.g. ALT
Test results Certain tests using same filter
Software 
Accidental result is abnormal
Maintenance
Troubleshooting
All results are low

Work flow
The added sample is insufficient ① Unclog the sample probe
Machine structure
or replace the probe
① The sample probe is clogged
② Replace the defective
Hardware system ② Fluid tubing has leakage tubing
Liquid system ③ The Sample syringe work ③ Make the syringe work
improperly normally
Optical system
ALL results The reaction is insufficient ① Adjust the mixing
Test results
are low ① The mixing bar is over high position of mixing bar
Software
② The mixing bar does not work ② Check the DC motor
Maintenance ③ Inspect the temperature

③ The reaction temperature is
abnormal
① Increase all backgrounds
The light intensity is too low by debug software
② Replace the lamp
Troubleshooting
Some results are high, some are low

Work flow The mixing bar strikes the
reaction cuvette Adjust the position of mixing bar
Machine structure
Hardware system
① Check Halogen lamp
Liquid system ② Check Optical Fibre
Some results Some backgrounds are ③ Check Filter
Optical system
are high, some abnormal ④ Check the Photoelectric sign
Test results are low al sampling position
Software
Maintenance Liquid system has

problems Check Syringe , tubing, Valve, Probe and
so on
Troubleshooting
Certain item results is abnormal

Work flow
Machine structure
Good repeatability
Calibrate this item again
Hardware system
Improper calibration parameters
Liquid system Certain item
results is Poor repeatability
Optical system ① Reagent is disabled
abnormal ① Change new reagent
② Bubbles in reagent bottle
Test results ② Remove bubbles
Software
Maintenance
Troubleshooting
Accidental result is abnormal

Work flow
Machine structure
Hardware system Normal in the repeated tests

① Reaction cuvette is dirty ① Do not use cuvette
Liquid system repeatedly
② There are bubbles aspirated
Optical system into reagent probe ② Remove the bubbles
Accidental
Test results result is
abnormal
Software
Maintenance
Abnormal in repeated tests. Chang the sample
The sample is abnormal
Work flow
Machine structure
Hardware system
Liquid system
Software
Optical system
Test results
Software
Introduction
Maintenance
Introduction
Software frame
System software/Debug software PC
Work flow
Machine structure
Hardware system
Main Unit
Liquid system
Refrigeration Unit
Optical system
Test results Sample Temperature Photoelectric mixing Unit

Reagent
Software Unit control Unit Unit
Unit
Maintenance
Control software
Analyzer
Introduction
Control software
•Functional Unit
 Main Unit
 Mixing Unit
Work flow  Reagent Unit
Machine structure  Sample Unit
 Temperature control Unit
Hardware system  Photoelectric Unit
Liquid system •Upgrade
Optical system
Test results
Software
Maintenance
Introduction
Debug software
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
• Single-step instruction and macro instruction operation

• Parameter enquiry and modifying
• Temperature monitor
• Photoelectric measurement
Introduction
Operation software
Work flow
Machine structure
Hardware system
Liquid system
Optical system
Test results
Software
Maintenance
Work Principle
Work flow
Machine structure
Hardware system
Maintenance
Liquid system
Optical system
Test results
Software
Maintenance
Trouble Shooting
Daily maintenance
Work flow
Machine structure
1. Clean sample disk and reagent disk
Hardware system
Liquid system
2. Clean surface of probe and mixing bar
Optical system 3. Clean all the covers
Test results 4. Check deionized water tank and waste water tank
Software
Maintenance
Trouble Shooting
Maintenance
Every 3 months: Every 6 months: Every year:

Work flow
Machine structure ① Unclog probes with

needle;
Hardware system
② Replace check valve ①Replace washers for
Liquid system probe and syringe;
if it is very dirty.
①Check the syringe
Optical system
plunger; ②Replace probe is
③ Replace 100μL recommended.
Test results
plunger assembly in
②Check all the valves
Software sample syringe ③Replace mixing bar is
preferred
Maintenance
④ Replace 500μL
plunger tip in
reagent syringe
End
Thanks

BS120 Service Traininig - 01.01.A

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BS-120 Service Training

International Service Dept.

Get new parts

•Optical system Structure

BS-120 Front View

BS-120 Side View

Reagent and sample tray of BS-120

Maintenance 36 position

Mixer Sample & Reagent Syringe

 Reagent/sample probe drive assembly

Machine structure  Mixing bar drive assembly

Optical system  Reaction tray assembly

Mechanical Structure of BS-120

Reagent and Sample Tray Assembly Reaction Tray Assembly

Mixing Bar Drive Assembly

Let’s try to adjust the position!

Reagent/Sample probe drive assembly

Test results Arm Base Assembly Reagent pre-heater assembly

Maintenance 4~15 ℃ around 35℃

Problems of Reagent pre-heater

Symptom Probable Cause

Symptom Probable Cause Corrective Action

Hardware system ① The cable is not well ① Reconnect the cable

Optical system Home position

Reaction tray assembly

Maintenance Base for the Reaction Segment

Reaction tray assembly

Work flow Temp Sensor

Optical system Photodiode Board

Temperature problems in Reaction tray

issues Probable Cause Corrective Action

Light Source Assembly

Light Source Assembly

Replacing the Lamp….

Light Source Assembly

Test results Connection,

Machine structure Refrigeration

Power Drive Board

FPGA Download Interface

Serial Port ISE Module

Main Board (CPU Board)

Work flow Functions:

Jumper on Main Board

Power Drive Board (PDB)

Power Drive Board of BS120

V-Motor of Probe Power

Reaction-Temperature Sampling Board

A/D Conversion Board

Photoelectric Conversion Board

Software Also called pre-amplification board.

Reagent Refrigeration Board

Work flow J71~74 Fans for J89 Peltiers

J86 12V/5V Board

Reagent Refrigeration Board

Work flow Functions:

Reagent Refrigeration Board

Liquid Level Detection Board

VR1 Adjust Point

Liquid Level Detection Board

Software TP2 Voltage TP6 Anti-Collision

Power supply assembly

LAMP A12V B12V