The effects of Dendrobium growth under different

concentrations of activated carbon and day length

Li, Hsin

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CONTENTS
01 Abstract

02 Introduction

03 Material and method

04 Result

05 Conclusion

06 References
Abstract

• The purpose of this study was investigated that effect of activated carbon (AC) a
nd light on in vitro proliferation of Dendrobium spp.. AC is often added to the me
dium to improve the plant growth, and the light also is the most important factors
affecting plant growth. For this study the 0.5 -1cm nodal explants were used and c
ultivated in MS medium additive different AC concentrations and given light or d
ark environments to explore its effects for the proliferation and plantlet growth.
Introduction of Dendrobium
• Is a fairly large genus under Orchidaceae.
• Dendrobium is usually divided into spring and autumn
• Native all over Asia,
• Growth on the trunk or tree hole.
• Colors are very diverse, but mainly white and reddish purple.

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Introduction of Heuchera micrantha
• Drought-tolerant and adaptable
• Growth divided into four stages, spring to summer for the growth period, autumn f
or mature period, winter for dormancy period, blooming in spring or in autumn.
• Daylight : Compared with general orchids, it need more daylight.
• Growth temperature is 18-30 ℃, but sometime even can grow well when the temp
erature more than 30 ℃.
• Reproduction: TC, cuttings, buds.
Introduction of activated carbon (AC)

Function
• Inhibit or promote bud and stem growth.
• Speed ​up reproduction .
• Establish a darkened environment to improve rooting.
• Adsorption some undesirable or inhibit substance.
• Prevent plant browning
Material and method
Take the young stem, cut from the top buds where down to 3-4 nodes, and remove t

he leaves and leaf tips, and use them after sterilized.

According to the test requirement, add different concentrations of activated carbon

(AC), and place them in a bright environment or in a dark environment to compar

e the growth.
MS medium formula (A soulation)
Material and method - Plant material

Stems were washed with 75% ethanol to remove surface contamination and then
sterilized by 1% sodium hypochlorite and one drop of Tween-20 then shaking tub
e by 10 minutes to sterilize.

The stems were then rinsed three times with sterile distilled water in laminar flow
to remove minor amounts of disinfection liquid. and cut the stems for use in vitro.
Material and method - Culture media and con
ditions
For germinating, the seeds were cultured in mugs on 10 ml of ½ MS medium, w

hich containing 0.03 % (m/v) sucrose and 0.0075% (m/v) agar.

Cultures were incubated in a growth chamber which at temperature of 25°C, a 12h

photoperiod provided.
Method
Method - Experimental design
AC concentration
NO add AC Add 1g/l AC Add 2g/l AC
Light
environments

NO add AC Add 1g/l AC Add 2g/l AC

Dark
environments
Results
Method - Shoot multiplication
First cut the stems into 1-0.5 cm length for each stem, and cultured on ½ M
S medium with added different concentrations of AC (0, 1, and 2 mg/l) to c
ombined with two environment light or dark.
Result
• Stem explants from grown plants of Dendrobium were inoculated onto the 1/2M
S mediums with different concentrations AC and combinations of light or dark en
vironments .
• L+AC / L-AC

• D+AC/ D-AC
(L= light environments, D= dark environments.)

To observe the effects of different combinations and concentrations of

different types of AC on buds, stem length, and rooting.
Result- Literature result
Conclusion
Conclusion
• Activated carbon has many pores that gives it a large internal surface area t

hat allows it to adsorb many substances. It is commonly used in tissue cultu

re to promote cell growth and development, plays an important role in micr
o propagation, like orchid seed germination, somatic embryogenesis, rootin
g, stem elongation and bulb formation.
The addition of activated carbon to the culture medium may cause beneficial
or harmful effects, such as adsorption of a toxic substance, causing a dark
environment of the medium, adsorption of plant growth regulators or other
organic compounds, improvement of the aeration of the medium, and growth
promoting substances that adsorb the release of the plant or toxic chemicals.
In general, the addition of activated carbon can promote the plant formation
and growth. Sometime the plant will injured in cutting, it will cause the plant
be browning and secretion of some substances into the medium, and it will
cause the plant is blocked and the growth is slow, but if add the activated
carbon into medium, it can prevent those problem. Adding activated carbon
and dark environment treatment are both give dark environment to promote
roots, so the number of scales and root length can be better.
Reference
• 文心琪。 2015 。竹的再生利用—活性碳對植物組織培養之影響。
• 李玲嬌。 2008 。銀柳微體繁殖之研究。國立宜蘭大學園藝學研究所
• 張盛添、王才義。 1991 。孤挺花組織培養之研究。桃園區農業改良場研究
彙報 , (37)
• 陳美齡。春石斛蘭莖節培養技術之介紹。中華民國一○二二年八月臺中區農
情月刊【第一六八八期】第四版農業新知專欄
• M.J. Pan, J. van Staden. 1998. The use of charcoal in in vitro culture. Plant Growth
Regulation December 1998, Volume 26, Issue 3, pp 155–163
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•