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  • 150 Phage Growth Curve

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    Mashal Wakeela
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    A phage growth curve consists of four steps: 1) inoculation where phages attach to host cells and their numbers decrease, 2) eclipse where phages replicate inside host cells causing titers to decrease to zero, 3) burst where host cells lyse and release many new phages causing titers to rise steeply, and 4) burst size where the number of phages released per cell becomes constant.

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    A phage growth curve consists of four steps: 1) inoculation where phages attach to host cells and their numbers decrease, 2) eclipse where phages replicate inside host cells causing titers to decrease to zero, 3) burst where host cells lyse and release many new phages causing titers to rise steeply, and 4) burst size where the number of phages released per cell becomes constant.

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    9 views9 pages

    150 Phage Growth Curve

    Uploaded by

    Mashal Wakeela
    A phage growth curve consists of four steps: 1) inoculation where phages attach to host cells and their numbers decrease, 2) eclipse where phages replicate inside host cells causing titers to decrease to zero, 3) burst where host cells lyse and release many new phages causing titers to rise steeply, and 4) burst size where the number of phages released per cell becomes constant.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 9

    Virology

    Phage Growth Curve


    Phage Growth Curve
    GRAPH
    • Different from bacterial

    growth

    • Not sigmoidal curve

    • Consists of four steps


    Phage Growth Curve
    STEPS
    1. Inoculation
    2. Eclipse

    3. Burst

    4. Burst size
    Phage Growth Curve
    Phage Growth Curve
    INOCULATION/ ATTACHMENT
    • Virus in blood stream
    attaches to host cell

    • Beginning to decrease in
    number (negative
    gradient)
    Phage Growth Curve
    Eclipse/ Latent Period

    More and more viruses


    replicate

    Thus virus titre decreases

    Reaches at 0 gradient

    No virus in external medium

    6
    Phage Growth Curve
    BURST
    • Virions released

    • Host cell releases many viral


    particles

    • Host cells lyse

    • Very steep rise in viral

    titre
    Phage Growth Curve
    Burst Size

    • Number of virions
    released per bacterium

    • Becomes constant
    Phage Growth Curve

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