TISSUE PROCESSING:

DEHYDRATION
INTRODUCTION TO TISSUE
DEHYDRATION

BY
TOMORI MARYAM OLUWAPELUMI
BMS1701981
 Dehydration is:

• Done after fixation and before clearing of the tissue.

• Removal of water from aqueous fixed tissue.

• Necessary to prepare the tissue for embedding in non-

aqueous media.
DEHYDRATIN
G AGENTS

ELUEME FAITH

BMS1700098
WHAT IS A DEHYDRATING AGENT?
• Substance that dries or removes water from a
material.

Examples:
 Absolute alcohol

 Alcoholic solutions
FEATURES OF AN IDEAL
DEHYDRATING AGENT
• Rapid in dehydrating
• Less shrinkage or distortion
• Slow evaporation
• Should dehydrate fat tissues
• Should not harden tissues excessively
VARIOUS DEHYDRATING AGENT
• Ethyl Alcohol
• Isopropyl Alcohol
• Methanol
• Butyl Alcohol
• Acetone
• Cellosolve
• Dimethoxy propane
• Dioxane
•
ETHYL ALCOHOL (ETHANOL)
• Non Toxic
• Miscible with water
• No shrinkage
• Ideal for delicate tissue
• May cause hardening of tissue
• Highly inflammable
ACETONE
• Rapid Dehydration
• Miscible with water and organic solvents
• Extremely Volatile
• Highly inflammable
METHOD OF DEHYDRATION

BY

AJAYI DAMILOLA JOSIAH

BMS1701912
METHOD OF DEHYDRATION CONT’D

• Treat tissue with suitable dehydrating agent.

• Alcohol replaces the water in the tissue.

• Alcohol (in grades) penetrates the tissue.

METHOD OF DEHYDRATION CONT’D

Grades of alcohol used are as follows:

• 70%
• 80%
• 90%
• And then absolute alcohol in that order.
METHOD OF DEHYDRATION CONT’D
Factors to be considered in this step include:

• Time factor

• Shrink factor.

• Temperature factor.
IMPORTANCE OF DEHYDRATION

BY

OREDIA OSAS DESTINY

BMS1701975
 IMPORTANCE OF DEHYDRATION
• Expulsion.

• Preparation.

• Penetration.

• Prevention.
THANK YOU.