INSTITUTE OF HEALTH SCIENCE JENDRAL ACHMAD YANI

Public Health Program (B.Sc)

The Neem Seeds (Azadirachta indica A.Juss) Ekstract Such

as Natural Incecticide to Growth
Aedes aegypti Larvae

FEBRY ISTYANTO
113214094
The Neem Seeds (Azadirachta indica
A.Juss) Ekstract Such as Natural
Incecticide to Growth
Aedes aegypti Larvae
 BACKGROUND

Dengue Virus Aedes aegypti The sintetic chemystry

New Problem

Resisten

Alternatif

Ecdison Bloker Azadirachtin Neem Seed

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PROBLEM of IDENTIFICATION

How much concentration from

The Neem Seed (Azadirachta
indica A.Juss) Ekstract Such as
Natural Incecticide to Growth
Aedes aegypti Larvae ?

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 THE PURPOSE
General Purpose :To know The Neem Seeds
(Azadirachta indica A.Juss) Ekstract Such as Natural
Incecticide to Growth Aedes aegypti Larvae

Spesial Purpose :
• To Identify Lethal Concentration LC50 neem seeds
exstrct to Aedes aegypti larvae.
• To Know how the neem seeds extract to growth of
Aedes aegypti larvae
 GREEN FIEND
Effects on Insects
The growing accumulation of experience demonstrates that
neem products work by intervening at several stages of an
insect's life. The ingredients from this tree approximate the
shape and structure of hormones vital to the lives of insects
(not to mention some other invertebrates and even some
microbes). The bodies of these insects absorb the neem
compounds as if they were the real hormones, but this
only blocks their endocrine systems. The resulting deep-
seated behavioral and physiological aberrations leave the
insects so confused in brain and body that they cannot
reproduce and their populations plummet.

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 Conceptual of Framework

Neem Seed
Ekstrak Aedes aegypti
(Azadirachta Larvae
indica A. Juss)
HIPOTESIS

 Ho: The provision of an extract of neem seed has

not been affecting the growth of aedes aegypti
larvae
 Ha: The provision of an extract of neem seed has
been affecting the growth of aedes aegypti larvae
 Operational Definition
No Variabel Definisi Konseptual Definisi Operasional Cara Ukur Alat Ukur Hasil Skala

1 Ekstrak Biji Mimba Ekstrak yag dilakukan dengan Hasil rendaman biji Pengenceran Pipet Persen (%) Rasio
(Azadirachta indica A. cara direndam dalam air selama mimba yang diperoleh volum
Juss) 1 hari (Shultz,1992). dari bubuk biji mimba
kering yang direndam
air selama 24 jam
dengan konsentrasi
0%, 5%, 10%, 15%
dan 20%.
 

2 Larva Aedes aegypti Larva nyamuk yang mempunyai Sekumpulan larva Visual 1.
gigi sisir dengan duri samping Aedes aegypti Instar Makroskopis Pertumbuhan sesuai siklus Nominal
  (Sucipto,2011). III yang diberikan nyamuk
ekstrak biji mimba  
pada berbagai 2.
konsentrasi. Pertumbuhan terhambat
(gagal berkembang/mati/
tidak bisa menjadi nyamuk)
 

3 LC 50 biji mimba terhadap Kriteria yang digunakan untuk Dosis yang Regresi Probit   Konsentrasi (dalam %)  
pertumbuhan larva Aedes menginterpretasikan hasil dari menyebabkan
aegypti kematian organisme sebesar kematian pada 50%
50% (Sucipto,2011). larva Aedes aegypti.
 Population
The population come from all
over the larva of aedes aegypti
mosquito derived from the lab
parasitologi stikes general
achmad yani cimahi 
 Sample
Many unit experiment follows the rules of
replication according to gomez ( 1990 ) ,
meanwhile the number of sample following the
rules of roscoe ( 1975 ) namely by the number of
treatment ( t ) 5 times treatment , 3 times
repetition with a kind of instar 1 and 10 the tail of
larval concentration on each 0 , 5 percent , 10
percent , 15 % and 20 % , hence the number of
units experiment equal to 15 units of
experiment .So that the number of larvae a whole
a total of 150 larvae
Replication of Studies

(T-1)(R-1) > 6
(5-1)(R-1) > 6
4R> 10
R> 2.5
 Practice

Larva 3
@10

0% 5% 10% 20%
15%

Look and Write

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 GREEN FIEND

Analizing Data
Univariat
Bivariat
Probit Linear Regresion

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 Probit

Y=A+BX
Probit 50%=A+B(Log konsentrasi ekstrak)
Log konsentrsi ekstrak= (probit 50%-A)/B
LC50= Anti Log Konsentrasi Ekstrak
THANK YOU AND GOOD LUCK

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