EFFECT OF NEEM (Azidarachta indica) AND PAPAYA (Carica papaya) EXTRACTS TO

THE MORTATLITY RATE OF DIAMONDBACK MOTH (Plutella xylostella)

Arabella Morfe, Bridgette Arandila, Bry Umpacan, Cleford Aguirre, Dan Patrick Rapi, Honnah
Yohbel Galzote, John Rey Batan, Kim Andrew Camba, Rose Gallano, Reymart Pedrique,
Wilbert Jann Cabelin

I. RATIONALE

Pechay is also known as Bok choy or hosts, which can lead to death in the long
Pak choi in various countries, it has glossy term if nit prevented.
dark green leaves and sturdy crisp white or
Farmers consider the neem tree to be
green stalks in loose head. Brassica rapa
a natural pesticide source since the oil
subsp. chinesis is the scientific name for
extracted from its seed has anti-pesticidal
genus of cabbage in the mustard family. It’s
properties (Bond, 2012). This tree’s leaves
a typical ingredient in stir-fries, and its
also demonstrated that they might be used as
particularly popular in Asian cuisine
a natural insecticide. Wickison (2021)
(Britannica,2015).
described neem leaf extract as mosquito
Pechay is commonly framed in repellent with pesticide applications in
tropical areas like the Philippies, especially agriculture in an article.
because it is the most consumed green
Papapaya (Carica papaya) , on the
vegetable in the country due to its short
other hand, had already demonstrated its
growing time. It may be harvested in 30 to
aility to act as natural insecticide. The use of
40 days after sowing,giving it an advantage
papaya leaf extract solution on aphids was
over a variety of other leafy crops (Gonzaga
studied by Sunarti (2019).
et.al.,2017) Despite its quick harvesting
period, this crop is also quite nutritious. The researchers decided to use two
leaf extracts, namely neem and papaya leaf
Diamondback moths are often
extracts, to see if these two, alone or in
regarded as the most damaging insect pest of
combination, would have anti-pesticidal
Brassica crops worldwide. The larvae of this
properties against diamondback moths when
bug cause holes in the leaves of the plant
applied to pechay crops with the goal of
reducing the harmful effect of diamondback
moth on pechay crops.
II. RESEARCH PROBLEMS
This research seeks to determine if
the two extracts namely neem and papaya
extracts affects the mortality of
diamondback moths. Specifically, this study
seeks to answer the following questions:
1. Is there a significant difference in the
mortality rate of diamondback moth
between those applied with and without
neem extract.
2. Is there a significant difference in the
mortality rate of diamondback moth
between those applied with or without
papaya extract?
3. Is there a significant difference in the
mortality rate of diamondback moth
between those applied with or without
combined neem and papaya extract?
III. HYPOTHESES
1. There is no significance difference in the
mortality rate of diamondback moth
between those applied with and without
neem extract.
2. There is no significant difference in the
mortality rate of diamondback moth
between those applied with and without
papaya extract.
3. There is no significant difference in the
mortality rate of diamondback moth
between those applied with and without
combined neem and papaya extracts.
IV. EXPECTED OUTCOMES
It is expected that the use of neem
and papaya extracts as a botanical pesticide
to the use of inorganic pesticides in
controlling diamondback moth in the
petchay plant is projected to be effective.
V. METHODS
Collection and Making of Neem and
Papaya Extracts [CITATION Azw15 \l
1033 ]
The extracts were collected within
the locality. The neem leaf and papaya leaf
followed the pre-extraction preparation of
air-drying method where the samples are air-
dried for 7 days before extraction. The
extraction process took place at the Granja-
Kalinawan National High School Science
Laboratory, with direct supervision of the
research adviser. Fifty grams of papaya and
fifty grams of neem leaves was weighed Same steps stated above were

using a digital balance. After which, the considered. A total amount of 750 ml was

decoction process was done in different set- also extracted from the procedure. Just like

ups of different samples as follows: the neem extract, 250 ml was set aside for

Preparation of Neem Extract the Neem-Papaya extract.

In extracting for the neem leaves Preparation of Neem-Papaya Extract

decoction process was done. Eight-hundred The neem-papaya extract was

millimeters of water were measured using a prepared by combining 250 ml neem extract

laboratory beaker with a 1000 ml capacity. from the 750 ml neem extract and 250 ml

After which, the water was mixed with the papaya extract from the 750 ml neem

50 g of neem leaves in a beaker and was extract, garnering 500 ml of neem-papaya

heated using the hot plate with a 300°C extract.

temperature until it reaches its boiling point. Preparation of Controlled Variable

The set up was allowed to cool down for 24 The distilled water that will be used

hours and was kept in in a closed container. in the study was measured using a beaker. A

In separating the juices from boiled neem total of 500 ml of water was used as a

leaves a sifter was used. A total of 750 ml controlled variable in the study.

neem extract was gathered after procedure. The water and the gathered extracts
were transferred into four identical hand
The 250 ml of the extract was set aside for
sprayers.
the preparation of extracts for Neem-Papaya
VI. EFFICACY TEST [ CITATION Jac01 \l
extract.
1033 ]
Preparation of Papaya Extract
After preparation of extracts, the

researchers went to Granja-Kalinawan

National High School Extension, Jaro, Leyte considered, 12 pechay (Brassica rapa subsp.

where the infected pechay crops are located. Chinensis) were utilized in the test.

Prior to leading the test, each sample of

VII. DATA ANALYSIS
pechay (Brassica rapa subsp. Chinensis)
The study will use One-Way
was covered with a 15x15 inches framed net
Analysis of Variance (ANOVA) to test the
so that the diamondback moth would not be significant differences between the three set-

able to escape. A five-inch distance of the ups. The mortality rate results will also be
presented.
sprayer to the pechay (Brassica rapa subsp.

Chinensis) with diamondback moths VIII. BIBLIOGRAPHY

(Plutella xylostella) was maintained in each

sample and 5 sprays of each extract was

scattered from the spray bottles. From that

point forward, water was applied as a

controlled variable in the test. In each trial,

one pechay (Brassica rapa subsp.

Chinensis) with diamondback moth

(Plutella xylostella) was utilized for

different conditions where three trials were

made for the water and extracts separately.

Along these lines, in each trials of neem,

papaya, and a combination of both there

were 3 pechay (Brassica rapa subsp.

Chinensis) was used. With everything

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