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Recombinant Dna Technology1
Recombinant Dna Technology1
Technology
I. Manipulating Genes
DNA Technology: technology involved
in genetic engineering that can be used
to cure diseases, to treat genetic
disorders, and to improve food crops.
I. Manipulating Genes
A. Restriction Enzymes: bacterial enzymes that cut
long strands of nucleotides into smaller segments.
1. Recognize specific sequences of nucleotides (bases)
and cut the DNA at a specific site within the sequence.
2. In one chain, the sequence runs left to right and on
the complementary chain the sequence runs
right to left.
I. Manipulating Genes
3. Single chain “tails” of DNA called
sticky ends are created on each DNA
segment by the action of the restriction
enzymes.