MAJOR

EQUIMPEMENTS
FOR MICROBIAL
CULTURE

PRESENTED BY

ASHU CHATRA
DEPARTMENT OF BSC 1ST SEMESTER
BOTANY
 LAMINAR
AIRFLOW
CONTENTS BOD INCUBATOR
INCUBATOR
SHAKER
LAMINAR AIR FLOW
– DEFINITION
Laminar flow is defined as airflow in which the entire body of air within a
designated space is uniform in both velocity and direction.

Laminar Hood sometimes also known as Laminar Air Flow is an enclosed bench
designed to prevent contaminations like biological particles (SPM) or any
particle sensitive device. This closed cabinet is usually made up of stainless steel
without any gap or joints where spores might collect.

Air is drawn through a High Efficiency Particulate Air (HEPA) filter and blown
in a very smooth flow or streamline flow (when a fluid flows in a parallel layer
with no disruption between the layers)
COMPO A Blower/Motor system

NENTS
OF THE
Pre-Filter-A fibrous washable filter sheet with large pore size and folded setup for
high surface area to filter restrict the intake of suspended dust particles.

LAMINAR
AIR High Efficiency Performance Air filter

FLOW A Plenum Chamber (Pressurized housing containing air at

HOOD positive pressure)

Ultra Violet Light-ultra violet light is incorporated in the illumination panel of

laminar air flow to take care of the sterilization of the existing air present in the
enclosure, thus ensuring the high standard of cleanliness in equipment before the
commencement of actual working.
 For incubation of
APPROVAL DATE : samples for analysis
PURPOSE
JANUARY 2008 of biochemical
oxygen demand

BOD
INCUBATO
Inner size material stainless steel inside
SPECIFICATION approx. 0.5 * 0.5*0.8 chamber hot enameled
m (w*d*h) outside

R Range accuracy
Power supply display
220 VAC
5 to 50
+_25% ,50hz,approx,4 display
c,thermostatically
00watt digital LED
controlled+_0.5c
temperature
 High quality
insulation between
Shelves
inner chamber and
uter wall

Full length inner glass

door with latch for
Motorised blower for
monitoring without
uniform temperature
disturbing inside
temperature

Thermometer access
hole to display inside
Built in voltage
temperature with
suitable thermometer

FEATURES
INCUBATOR DEFINITION

An incubator is a piece of vital laboratory equipment

necessary for cultivating microorganisms under artificial
conditions.
An incubator can be used to cultivate both unicellular
and multicellular organisms.
This Modern Day Myth About the Ocean Is Just Plain
Wrongn incubator, in microbiology, is an insulated and
enclosed device that provides an optimal condition of
temperature, humidity, and other environmental
conditions required for the growth of organisms.
 Cabinet

Door
Control panel
Thermostat
Perforated shelves
Asbests door gasket
L shaped thermometer
HEPA filters
COMPONENTS/PARTS OF Humidity and gas control
INCUBATOR
 An incubator is based on the principle that microorganisms
require a particular set of parameters for their growth and
development.

All incubators are based on the concept that when organisms

are provided with the optimal condition of temperature,
humidity, oxygen, and carbon dioxide levels, they grow and
divide to form more organisms.

In an incubator, the thermostat maintains a constant

temperature that can be read from the outside via the
thermometer.

The temperature is maintained by utilizing the heating and no-

heating cycles.

During the heating cycle, the thermostat heats the incubator,

and during the no-heating period, the heating is stopped, and
the incubator is cooled by radiating heat to the surrounding.

PRINCIPLE/ WORKING Insulation from the outside creates an isolated condition inside
the cabinet, which allows the microbes to grow effectively.

OF INCUBATOR
 Once the cultures of organisms are created, the culture
plates are to be placed inside an incubator at the desired
temperature and required period of time. In most clinical
laboratories, the usual temperature to be maintained is 35–
37°C for bacteria.

Before using the incubator, it should be made sure that no

remaining items are present in the incubator from the
previous cycles. However, in some cases, if the same
incubator is being used for multiple organisms, and they
require the same set of parameters, they can be placed
together in the same incubator.

The door of the incubator is then kept closed, and the

incubator is switched on. The incubator has to be heated up
to the desired temperature of the growth of the particular
organism. The thermometer can be used to see if the
temperature has reached.
PROCEDURE FOR In the meantime, if the organism requires a particular
RUNNING AN concentration of CO2 or a specific humidity, those
parameters should also be set in the incubator.
INCUBATOR
 Benchtop incubators
CO2 incubators
Cooled incubators
Shaker incubator
Portable incubator

TYPES OF INCUBATORS
Incubators have a wide range
of applications in various
areas including cell culture,
pharmaceutical studies,
hematological studies, and
biochemical studies.
Some incubators are used to
increase the growth rate of
organisms, having a
prolonged growth rate in the
natural environment.
USES OF INCUBATOR
Some of the uses of
incubators are given below:
Specific incubators are used
for the reproduction of
microbial colonies and
subsequent determination of
biochemical oxygen demand.
Incubators are used to grow Incubators can also be used
microbial culture or cell to maintain the culture of
cultures. organisms to be used later.
Incubators also provide a
These are also used for controlled condition for
breeding of insects and sample storage before they
hatching of eggs in zoology. can be processed in the
laboratories.
The following precautions are to be followed while running an incubator:

As microorganisms are susceptible to temperature change, the fluctuations in temperature of the cabinet by repeatedly opening the door should be
avoided.

The required parameters growth of the organism should be met before the culture plates are placed inside the cabinet.

The plates should be placed upside down with the lid at the bottom to prevent the condensation of water on to the media.

The inside of the incubators should be cleaned regularly to prevent the organisms from settling on the shelves or the corners of the incubator.

While running the incubator for an extended period of time, sterile water should be placed underneath the shelves to prevent the culture media from
drying out.

PRECAUTIONS
THANK
YOU