AI- cytology

assessment of
hepatic lipid
content in dairy
cattle
Project Description
Brief description of the project
Project Scope
 Describe the work to be accomplished
A device (software+ hardware)  able to recognize hepatic lipid content from fine needle
biopsy liver samples, on field using cytology as its main principle. Study findings [1] 
support that FNB (fine needle biopsy) liver cytology is a practical, minimally invasive
addition to the diagnostic toolkit for veterinary dairy herd health management,
particularly with regard to Hepatic Lipid content. 
 What’s the purpose or business need for this project?
Clinical abnormalities associated with Hepatic Lipidosis in dairy cattle—including
metritis, mastitis, ketosis, abomasal displacement, and decreased reproductive
performance—result in decreased productivity and major economic losses; annual costs
of fatty liver in dairy cows in the United States have been estimated to be more than $60
million. In addition there is not many methods to assess hepatic lipid content om field.
Project Scope

 Is there a relationship to other projects?

Chemical extraction of triglyceride concentration from liver tissue remains the
gold standard for quantifying hepatic lipid content, but mass spectrometry is not
currently diagnostically practical.
Studies [1] also sought to compare the diagnostic performance of liver cytology, plasma
non-esterified fatty acid concentration ([NEFA]), and plasma β-hydroxybutyrate
concentration ([BHB]) derived from a measurement performed on whole blood, for
assessing HLC.  At present, there is no reliably accurate blood test, or combination of
tests, to quantify hepatic lipid content (HLC) or diagnose HL in cattle
Hepatic lipid content can be estimated in various ways, such as histologically using lipid-
soluble stains  by flotation of biopsy specimens in copper sulfate solutions of different
specific gravities, or ultrasonographically
Project Scope

 Who are the stakeholders?

Farmers and Veterinarians
 What work is out of scope for this project?
This project is not meant to focus on other cytological lesions of the liver other than
hepatic lipid content.
Objectives
 Identify overall project objectives:
 Technical objectives
 Schedule objectives
 Cost objectives
 Special objectives

 Non-goals
Deliverables
 What products or services will your project deliver?
 Include client requirements
Success Factors
 Identify elements that are key to the success of the project, such as:
 Satisfied clients or stakeholders
 Met project objectives
 Completed within budget
 Delivered on time
Project Team Roles and Responsibilities

[Full Name]
Project Manager

[Full Name]
Administrative
Assistant

[Full Name] [Full Name] [Full Name] [Full Name]

Member Role Member Role Member Role Member Role
Implementation
 Tasks/activities
 Procedures
 Tools/technology
 Project change control process

DYA: define your acronyms!

Resources

Outside Manufacturin
People Equipment Locations Sales
Services g

Resource 1 Resource 1 Resource 1 Resource 1 Resource 1 Resource 1

Resource 2 Resource 2 Resource 2 Resource 2 Resource 2 Resource 2
Resource 3 Resource 3 Resource 3 Resource 3 Resource 3 Resource 3
Resource 4 Resource 4 Resource 4 Resource 4 Resource 4 Resource 4

Notes Notes Notes Notes Notes Notes

Cost Analysis
Annual Cost
$1,400,000

$1,200,000

$1,000,000

$800,000

$600,000

$400,000

$200,000

$0
Parts and ma- Manufactur- Salaries Maintenance Office lease Warehouse Insurance Benefits and Vehicles Research
terials ing equipment lease pensions
Project Schedule and Milestones

Milestone 1 Milestone 2 Milestone 3 Milestone 4

• January 10 • April 10 • July 10 • October 10
Risk Management Plan
Risk Probability Impact Owner Mitigation Plan

Budget cuts may

reduce staff, affecting Project See appendix for a phased
project scope and Medium High Manager implementation plan
schedule
Quality Management and Performance Measures
 Define quality management plans
 How will you monitor and control costs?
 How will you monitor and control schedule?
Appendix
 Reference supplementary materials
and resources