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    Santosh Rananaware
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    The document contains data from experiments measuring the activity of different activators (S12A, S12B, S12C) and targets (ssRNA Target A, ssRNA Target B, ssDNA Target C) compared to a no activator control. It shows that S12A had the highest activity for ssRNA Target A with a fold change of 24, S12B had the highest activity for ssRNA Target B with a fold change of 55, and S12C had the highest activity for ssDNA Target C with a fold change of 30 compared to no activator control. Additional data is shown measuring the binding of different sequences (TTTA, AAAT, VVVN) to proteins (Lb,

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    The document contains data from experiments measuring the activity of different activators (S12A, S12B, S12C) and targets (ssRNA Target A, ssRNA Target B, ssDNA Target C) compared to a no activator control. It shows that S12A had the highest activity for ssRNA Target A with a fold change of 24, S12B had the highest activity for ssRNA Target B with a fold change of 55, and S12C had the highest activity for ssDNA Target C with a fold change of 30 compared to no activator control. Additional data is shown measuring the binding of different sequences (TTTA, AAAT, VVVN) to proteins (Lb,

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    6 views5 pages

    Mutants (Autosaved)

    Uploaded by

    Santosh Rananaware
    The document contains data from experiments measuring the activity of different activators (S12A, S12B, S12C) and targets (ssRNA Target A, ssRNA Target B, ssDNA Target C) compared to a no activator control. It shows that S12A had the highest activity for ssRNA Target A with a fold change of 24, S12B had the highest activity for ssRNA Target B with a fold change of 55, and S12C had the highest activity for ssDNA Target C with a fold change of 30 compared to no activator control. Additional data is shown measuring the binding of different sequences (TTTA, AAAT, VVVN) to proteins (Lb,

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    WT SAHARA

    M01
    M02
    M03
    M04
    M05
    M06
    M07
    M09
    M09
    M10
    M11
    M12

    0 1 2 3 4 5
    Fold normalized to WT activator
    S12A S12B S12C No S12

    to no activator control
    Fold Change normalized
    ssRNA Target A 24 2 6 0

    40

    ssRNA Target B 2 55 9 0

    20

    ssDNA Target C 3 0 30 0

    0
    Lb As Er
    5×10 5 5×10 5 5×10 5

    4×10 5 4×10 5 4×10 5

    3×10 5 3×10 5 3×10 5


    RFU

    RFU

    RFU
    2×10 5 2×10 5 2×10 5

    1×10 5 1×10 5 1×10 5

    0 0 0
    TTTA AAAT VVVN TTTA AAAT VVVN TTTA AAAT VVVN
    Er Er
    1.5×10 6 25 nM HCV-RNA 4×10 5
    25 nM HCV-RNA
    NTC
    NTC

    3×10 5
    1×10 6
    RFU

    RFU
    2×10 5

    5×10 5
    1×10 5

    0 0
    50 25 12.5 6.25 3.12 1.5 0.78 0 1560 780 390 195 100 50 0 S12
    Concentration (nM) Concentration (pM)

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