PRONIOSOMES:A NOVEL APPROACH FOR DRUG DELIVERY

SYSTEM
A project report submitted to the
Rajasthan University of Health Sciences, Jaipur (Rajasthan)

In partial fulfillment for the award of the degree of

BACHLOR OF PHARMACY
[2021-22]

Supervised By- Submitted By-

Mr. Shankar Lal Soni Gaurav Shukla
[Associate Professor] B. Pharmacy
Arya College of Pharmacy Semester 7 th
Department of Pharmaceutics Enroll no. 2018/0114

ARYA COLLEGE OF PHARMACY, JAIPUR (RAJASTHAN) INDIA

SP-40 Kukas, Industrial Area, (RIICO), Delhi Road, Kukas, Jaipur (Raj.) 302028
Tel. No. – 0141-5148801, 5148802, 5148803, Fax. No. – 01426-510040
 Scheme Of Presentation

Introduction
Necessity
Advantages
Formulation Methodology
Characterization & Evaluation
Stability Studies
Application
References
 Introduction
 Proniosomes is dry formulation using a suitable carrier coated with non-
ionic surfactant and can be converted into niosomes immediately before
using by hydration.
 These proniosomes-derived niosomes are as good as or even better than
conventional niosomes.
 Approaches to stabilize niosomal affecting its properties of merits have
resulted in the development of promising drug carriers.
 Preparation of Niosomes from Proniosomes by
Hydration

 Prepared proniosomes powder is weighed and filled in

screw cap vials, water or saline at 80° C is added and
the vials capped.
 The vials are attached to a vortex mixer and agitated for
2 minutes to get niosomal suspension.
 Advantages

Proniosomes dry formulations of surfactant coated carrier- rehydrated by

brief agitation in water.

Minimizes problems of niosomes physical stability such as, aggregation,

fusion, and leaking of entrapped drug.

Additional convenience in transportation, distribution, storage and dosing.

Can carry both hydrophilic drug and hydrophobic drug.

Extensively used in various drug delivery systems like drug targeting

controlled release and permeation enhancement of drugs.

 Method of Preparation

I. Slurry method
II. Co-acervation phase separation
III. Slow spray-coating method
 Slurry Method

10 mg. Maltodextrin as a carrier

in 250ml RBF
+
Entire 14.5ml volume of surfactant
solution

Formation of slurry

Dry Proniosome
powder stored in
sealed container at
4°C
Co-acervation Phase Separation Method

Drug + Surfactant + Lipid in 5ml. Glass vial

+

0.5ml. Of Alcohol

Warm at 60-70°c for 5 min.

Mix all ingredients until dissolved

Warm on water bath Aq. 0.1% glycerol solution

A clear solution, converted to proniosomal gel on cooling

 Slow Spray Coating Method

 This method involves preparation of proniosomes by spraying

surfactant in organic solvent onto sorbitol powder and then

evaporating.
 The surfactant coating on the carrier is very thin and hydration of

this coating allows multilamellar vesicles as the carrier dissolves.

 The resulting niosomes are very similar to the conventional

niosomes but they are more uniformly distributed.

 Characterization of Proniosomes

Measurement of angle of repose

Scanning electron microscopy

Optical microscopy

Measurement of vesicle size

Drug content

Entrapment efficiency

In- vivo release studies

Stability studies
 Stability Studies

The International Conference on Harmonization guidelines (ICH) suggest

that stability studies for dry proniosome powders intended for reconstitution

should be conducted for accelerated stability at 40°C/75% relative humidity

according to international climates and climatic conditions (WHO, 1996).

For long-term stability studies, the temperature is 25°C/60% RH for Zone I

and II countries and 30°C/65% relative humidity for Zone III and IV

countries (RH) The product should be examined for its appearance, color,

pH, preservative content, particulate matter, sterility and pyrogenicity.

 Application
Sr. No. Purpose / Application Drugs studies
1. Transdermal drug delivery system Ketorolac
Estradiol
Captopril
Levonorgestrol
Frusemide

2. Inhalation therapy Cromolyn sodium

3. Enhancement of solubility Piroxicam

4. Improved permeability Alprenolol HCl

5. Prolonged release for improved anti- Ibuprofen

inflammatory activity
 Conclusion
Proniosomal systems have been widely accepted by
researchers and academics recently because of their ability to
deliver the drug to the desired organs and achieve the desired
effect with a smaller amount of drug and lower side effects.
Proniosomes are considered to be the better module for drug
delivery compared to liposomes and niosomes due to various
factors such as cost, stability, etc.
These systems have been shown to be more stable than
niosomes during sterilization and storage. The use of a
proniosomal carrier results in a high concentration of the
active ingredient(s), which is regulated by the composition
and its physical properties.
 References

Kakr R, Rao R, Goswami A, Nanda S, Saroha K. Proniosomes: An emerging vesicular system in drug
delivery and cosmetics. Der Pharmacia Lettre 2010;2:227-39.
Walve JR, Rane BR, Gujrathi NA. Proniosomes: A surrogate carrier for improved transdermal drug delivery
system. Int J Res Ayurveda Pharm 2011;2:743-50
 Mujoriya RZ, Dhamandeb K, Bodla RB. Niosomal drug delivery systems-a review. Int J Appl Pharm
2011;3:7-10
Nimbalwar M, Upadhyay K, Dixit G. Pharmacophore Int Res J. 2016; 7(2), 82-95
Pal P, Mishra S, World J Pharm Res. 2017; 6(5), 656-674
 Rao R, Kakar R, Anju G, Sanju N. Proniosomes: An Emerging Vesicular System in DrugDelivery and
Cosmetics. Der Pharmacia Lettre, 2010; 2 (4): 227-239.
Sudhamani T, Priya darisini N, A promising drug carriers, Int. J. Pharm. Tech. Res., 2 (2),2010, 1446-1454.
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