SEPARATION OF VARIOUS COLOURING

PIGMENTS FROM BOUGAINVILLEA FLOWERS

USING PAPER CHROMATOGRAPHY AND
COLUMN CHROMATOGRAPHY

•MEMBERS:
•Kishaya Bhattacharjee 22MSB0086 •Siya Soji 22MSB0082

•Ankita Nayak 22MSB0010 •Harsha Rai 22MSB0029

•Karingula Naveen 22MSB0085 •Ritwik Tripathi 22MSB0144

CHROMATOGRAPHY
• The separation of various pigments from an extract of flower petals.
• The pigments from an extract of flower petals can be separated by the process of
chromatography.
• The word chromatography is derived from Greek, chroma means “color”, and
graphein means “to write”
• During this process, the mixture is separated on a stationary phase(solid or liquid)
when a pure solvent or any gas is allowed to pass slowly over the stationary phase,
the different pigments of the extract travels at different speeds, causing them to
separate from one another.
• Therefore, chromatography method is used for the separation of different pigments
from an extract of flower petals of bougainvillea flower.
 • The chromatography technique is widely used to
separate, purify and identify compounds. Substance
balances in chromatography between a mobile phase and
a stationary phase. The more its movement the more the
interaction of the substance with the stationary phase.
• Paper chromatography is essentially a partition
chromatography. In paper chromatography, the stationary
phase is paper. Paper contains 22% of water molecules

Paper absorbed on about 78% of cellulose.

• Materials Required:

chromatography Whatman’s filter paper

Extract of leaves and extract of flowers
Chloroform/acetone
Methanol/Acetone
Rubber cork fixed with hook in the centre
Glass jar
Rubber cork fixed with hook in the centre
Test tubes
Distilled water
Petroleum ether
Coloumn
chromatography

Aim:The aim of the experiment was to

isolate and determine the pigments present
in flower extract from this was achieved by
using column chromatography. These
pigments were then analysed using UV
Spectrophometry.