Professional Documents
Culture Documents
OF
BACTERIA
EUKARYOTIC PROKARYOTIC
• Parasites, fungi • Bacterial cell
• Membrane enclosed • Do not contain
organelles organelles
• Cytoskeleton • Cell wall,
peptidoglycan
OPTICAL/LIGHT MICROSCOPE
FLUORESCENT MICROSCOPE
• Light of high intensity source excites a
fluorescent agent, which in turn emits a low
energy light of longer wavelength that
produces the image
Fluorescent microscopy
Dark-field microscopy
© Universities Press (India) Private Limited Universities Press
MICROSCOPY
ELECTRON MICROSCOPE
• Beam of electrons instead of beam of light
• Electron beam focused by circular
electromagnets - analogous to lens in light
microscope
• Object held in the path of electron beam
and produces an image which is focused on
a screen
• Shadow casting
• Negative staining – phosphotungstic acid
• Freeze etching
• Differential stains
GRAM STAIN – Christian Gram (1884)
• Principle: Gram positive – acidic protoplasm
- retain basic primary dye
• Peptidoglycan of Gram-positive bacteria
thick – retain the dye iodine complex
• High lipid content of Gram-negative
bacteria makes them permeable to
counterstain (secondary dye)
CELL ENVELOPE
• Outer membrane (GNB)
• Cell wall – peptidoglycan
• Periplasm (GNB)
• Cytoplasmic membrane
• Bilayered
• Composed of lipopolysaccharide (LPS)
• Gives GNB - net negative charge
• Scattered throughout LPS - porins
• Porins - control passage of nutrients and
antibiotics
• Composed of disaccharide-pentapeptide
• Disaccharide-N-acetyl glucosamine
N-acetyl muramic acid
• Amino acid only linked to N-acetyl muramic
acid
• Polymers-crosslink-via peptide bridges to
form peptidoglycan sheets
•Transport of solutes
•Enzymes involved in cell synthesis
•Generation of chemical energy
•Cell motility
•Chromosomal segregation
• Capsule
• Fimbria-pili
• Flagella
• Role in causing infection
• Help identification in laboratory
SIGNIFICANCE
•Virulence - Inhibit phagocytosis
- Protect cell from lysozyme
•Permit adherence - Cell surface
Example: implant, catheters
•Prevents cell from drying
•Toxicity to host cell
•Protects cell from bacteriophage
SIGNIFICANCE
• Act as adhesins - bacteria colonise
• Receptor for bacteriophage
• Streptococcus pyogenes - M protein
• Virulence factor
• Some fimbriae - agglutinate RBCs
• Complex structure
• Composed of protein flagellin
• Embedded in cell envelope
• Motility - survival
• Non-contractile
• Single protein subunit - flagellin
• Anchored to bacterial cytoplasmic
membrane by disc-like structure