Professional Documents
Culture Documents
& DISINFECTION
M.T.MAREMA
PRINCIPAL TECHNICAL OFFICER (EH)
TUTUME DHMT
22- FEB 2022
Disinfection Sterilization
• Eliminates pathogens all • Annihilates all forms of
pathogens but 1 microbial life
• Not bacterial spores • Sterilizer/autoclave
• Chemical/physical
Importance of environmental cleaning
• One of the IPC Standard Precautions
• Cuts transmission of disease
• Prevents cross-contamination from inanimate objects
• Most pathogens exist in the environmental surfaces
• Most pathogens susceptible to env. Cleaning &
disinfection
PRINCIPLES OF ENVIRONMENTAL
CLEANING
• 1st step is cleaning
• Neutral Cleaning detergent so not to affect disinfectant
• Preparation according to manufacturer’s instructions
• Correct dosages/concentration
• From least to most contaminated
• From higher to lower levels
• From low touch to high touch surfaces
• From general patient area to isolation area
• Discard solutions after each use
• Avoid mists/aerosols dust generating activities
• Routine microbiological monitoring
Environmental cleaning supplies and equipment
STEP 5
disinfect
the surface
To prevent contamination and maintain efficacy of the disinfectant, change the disinfectant
solution and gloves if soiled.
EXAMPLE OF CLEANING FLOOR
Sample recommended cleaning frequency (Liberia)
Item Frequency
Surfaces • At least twice daily
(floors, tables, chairs) • When visibly soiled
Medical equipment • After every patient
(axillary thermometers)
- Performance observation
- Visual assessment
- Fluorescent markers (e.g., UV visible