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  • Depolymerase and Synthase Assays For Candidate Isolates

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    Susana García Sánchez
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    The document summarizes the results of a depolymerase assay performed on MAP plates using candidate marine isolates and control species to test for polyhydroxyalkanoate (PHA) synthase activity. The assay tested 8 candidate isolates and 5 control species at various dilutions on the MAP plates and measured PHA synthase activity by exposure time needed to detect fluorescence from Nile red staining at 356 nm using an Image Quant LAS 500 machine.

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    PHA assays

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    Depolymerase and synthase assays for candidate isolates

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    The document summarizes the results of a depolymerase assay performed on MAP plates using candidate marine isolates and control species to test for polyhydroxyalkanoate (PHA) synthase activity. The assay tested 8 candidate isolates and 5 control species at various dilutions on the MAP plates and measured PHA synthase activity by exposure time needed to detect fluorescence from Nile red staining at 356 nm using an Image Quant LAS 500 machine.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    14 views2 pages

    Depolymerase and Synthase Assays For Candidate Isolates

    Uploaded by

    Susana García Sánchez
    The document summarizes the results of a depolymerase assay performed on MAP plates using candidate marine isolates and control species to test for polyhydroxyalkanoate (PHA) synthase activity. The assay tested 8 candidate isolates and 5 control species at various dilutions on the MAP plates and measured PHA synthase activity by exposure time needed to detect fluorescence from Nile red staining at 356 nm using an Image Quant LAS 500 machine.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 2

    Depolymerase assay on MAP plates for candidate

    isolates (Anna´s) and control species 190523


    Dil 1 ½ 1/20 1/200 Dil 1 ½ 1/20 1/200
    Dil 1 ½ 1/20 1/200
    5 C
    1
    6 M
    2
    7 P
    3
    8 V
    4
    C A
    C

    Marine isolates Control species (CECT)


    1 = T3-B1 B colony (P. hodoensis) C = Cobetia marina
    2 = T3-B1 C colony (P. hodoensis) M =Marinobacter hydrocarbonoclasticus
    3 = T3-A11 (V. kanaloae) P = Pseudoalteromonas haloplanktis
    4 = T3-C1 Psedomonas-like colony V = Vibrio proteolyticus
    5 = T3-C1 Bright colony A = Alcanivorax borkumensis
    6 = T3-B9 Yellow colony (M. agarilyticus)
    7 = T3-B9 White colony (M. agarilyticus)
    8 = T3-C2 (R. atlantica)
    PHA synthase activity on MAGR (MA + 2%Glc + NileRed) plates, 30ºC 72h
    19/05/23
    Expossure time
    (356 nm in Image Quant LAS 500, GE)

    1 sec

    1.5 sec

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