Bioactive

Platelet Agregate
Nicco Marantson, drg
Annet Nabila, drg
Gatot Baydowi, drg ., Sp.BM
Introduction
• The main goal of the modern surgery is to get a higher rate of clinical
healing with least invasiveness procedures. In the last years, the
concept of regenerative surgery has been introduced
• Bone Engineering concept  scaffold, cell and growth factor
• It promotes sequence of biologic events, such as cell migration,
adherence, growth and differentiation. It’s a potential factor to
increase the success and predictability of regenerative procedures
Introduction
• Platelets play a crucial role in hemostasis and wound healing as they
are rich source of growth factors.
• They play a main role on cell migration, cell proliferation and
angiogenesis in tissue regeneration phase. These growth factors are
mainly located in blood plasma and platelets
PRP (Platelet Rich Pasma)
• The Platelet Rich Plasma (PRP), also termed
autologous platelet gel (Autologous Platelet Gel),
is a gel at high concentration of autolo- gous
platelets suspended in a small amount of plasma
after centrifugation of the blood of the patient.
• Whitman et al, 1997  using xenomaterial
• Marx, 1998  Using autologous
• Tube to collect blood  citrate–phosphate–
dextrose solution (as anticoagulant) , and need
bovine thrombin as clotting factor
• Its need at least 30 min preparation
PRGF (Plasma Rich In Growth Factor)
• Anitua developed plasma rich in growth factors
(PRGF) by modifying the procedure of PRP
preparation. It simplified the preparation
protocol and replaced animalderived thrombin
with calcium for clotting.
• Calcium Chloride is used as clotting factor
• Preparation time 10 minutes
PRF (Plasma Rich Fibrin)
• Prf: It was first developed by Choukroun in
france in 2001. PRF consists of an
autologous leukocyte platelet rich fibrin
matrix
• PRF composed of a tetra molecular
structure, with cytokines, platelets,
cytokines, and stem cells within which acts
as a biodegradable scaffold
• Requires only centrifuged blood without
any addition of anticoagulant and bovine
thrombin
CGF (Concentrated Growth Factor)
• It was introduced by Sacco in 2006. It is essentially an
upgraded version of PRF with a strengthened fibrin
matrix and boosted growth factors and cytokines.
• CGF technology has an interesting characteristic ; i.e. the
easy and speedy one-step preparation of larger, denser
and richer growth factors fibrin matrix than the other
solid PRPs
• Silica coated tube  CGF
• Plain tube  AFG
Comparison
Preparation
Thank You