MT 310 LAB

Clinical Chemistry

“Laboratory Safety”

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
 MOTIVATION
<<Just delete the Motivation Text on top. You can just readily
paste on this slide the picture, quote, or type the mechanics of
your game, link to a video, etc.>>

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
 OBJECTIVES
At the end of the activity the students shall be able to:

1. Recall how to use the NFPA diamond

2. State the general rules for working safely in a chemical
laboratory environment
3. Describe the possible routes of exposure for a hazardous
material
4.Answer correctly the research questions
Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
 LABORATORY SAFETY
I. CONTROL OF BIOHAZARDS
a. Handwashing
visibly soiled - 1-2 minutes/soap
and water

not visibly soiled - 30

seconds/alcohol-based cleaners

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
b. PPE
Sequence of Donning: gown, mask, safety goggles, mask

Sequence of Doffing: gloves, goggles, gown, mask

c. Decontamination
10% sodium hypochlorite or bleach
Contact time: 20 minutes
Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
d. Biological Safety Cabinet
Class Description Use
I Air passes through 1 hepa filter Minimal PPE
Doesn’t protect work surface
II Air passes through 2 hepa filters Most commonly used
A1 = 70% recirculated, 30% exhausted into the room Protection for both worker and work
A2 = Recommended for COVID-19 RT-PCR surface
B1 = 30% recirculated, 70% exhaustes
B2 = 100% air exhausted outside the building

III Completely encosed with glove ports For extremely hazardous organisms
Equipped with Negative pressure

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
II. CHEMICAL HAZARDS
 MSDS (Material Safety Data Sheets)
 TLV (Treshold Limit Value)
 NFPA (National Fire Protection Association)
- Standard Hazards Identification System
- Diamond Hazard Symbol

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
 DEGREES OF
HAZARD
0 = None Hazardous
1 = Slightly Hazardous
2 = Moderately
Hazardous
3 = Seriously
Hazardous
4 = Extremely
Hazardous
Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
III. RADIATION HAZARD
TYPE WAVELENGTH EXAMPLE
ULTRAVIOLET < 400 nm Germicidal lamps
VISIBLE 400-700 nm General Illumination
SPECTRUM
INFRARED > 700 nm Heat lamps, lasers
MICROWAVES 3 um - 3 mm Microwave energy beam
LOW FREQUENCY > 1 cm Radiofrequency coil in ICP-
MS
Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
IV. HEALTH CARE WASTES
TYPE OF WASTE COLOR CONTAINER
Non- infectious dry waste BLACK
Non- infectious wet waste GREEN
Infectious pathological waste YELLOW
Chemical/Pharmaceutical waste YELLOW WITH BLACK BAND
Radioactive wastes ORANGE
Sharps and Pressurized RED
containers Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
FIRE HAZARD

PASS RACE
Pull the pin Rescue
Aim at base of fire Alarm
Squeeze handle Contain
Sweep nozzle side to side Extinguish/Evacuate

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
BIOHAZARDS

I. SHARP HAZARDS
II. CHEMICAL HZARDS
III. RADIOACTIVE HAZARDS
IV. ELECTRICAL HAZARDS
V. FIRE/EXPLOSIVE HAZARDS
VI. PHYSICAL HAZARDS

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
BLOOD COLLECTION AND SPECIMEN CONSIDERATIONS

STOP YELLOW - SPS PUT GOLD - GEL + ANTICOAGULANT

LIGHT GREEN
LIGHT LIGHT BLUE - 3.2% SODIUM GREEN GREEN - HEPARIN (LITHIUM HEPARIN,
CITRATE SODIUM HEPARIN)
RED RED: PLASTIC - NONE LIGHT LAVENDER - K2EDTA
GLASS - CLOT ACTIVATOR
(SILICA)
STAY RED & BLACK/ GOLD - GEL + CLOT GO GRAY - SODIUM FLUORIDE
ACTIVATOR

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
BLOOD COLLECTION AND SPECIMEN CONSIDERATIONS:
CAPILLARY PUNCTURE
1. BLOOD GASES
2. SLIDES/ SMEARS
3. EDTA
4. OTHER ADDITIVE TUBES
5. SERUM

Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato
Thank You for
Listening!
Prepared by | Althea B. Cazar, RMT

St. Alexius College

Medical Technology Department
City of Koronadal, South Cotabato