Oral lactobacillus species and

their probiotic capabilities in

patients with periodontitis and
periodontally healthy
individuals
ARGHAVAN et al
CLINICAL AND EXPERIMENTAL DENTAL
RESEARCH,2023.
 INTRODUCTION :
• DENTAL CARIES and
PERIODONTITIS :
• Role of Porphyromonas
gingivalis.
• Association of Periodontitis with
Systemic conditions.
• Association between pathogenic
bacteria and beneficial bacteria.
• Use of new bacterial
treatments.
• Probiotics.
• Lu et al (2021).
• Bustamante et al (2020) ; Shimauchi et al (2008) and
Teughels et al (2013).
• Shokryazdan et al (2014).
• Cuozzo et al (2000) and Hirasawa and Kurita – Ochia
(2020).
• Terai et al (2015).
• Kolenbrander et al (2002).
• Terai et al (2015).
• Abdel-diam et al (2012; Bosch et al (2012); Hiraswa
& Kurita-Ochia (2020).
• Raghuwanshi et al (2015).
 AIM:
The aim of the study is to evaluate
oral lactobacillus species and their
probiotic capabilities in patients
with periodontitis and
periodontally healthy individuals.
 MATERIALS AND METHODS:
• 1.STUDY SUBJECTS { INCLUSION CRITERIA;EXCLUSION CRITERIA}.
• 2.COLLECTION OF ORAL SPECIMENS AND ISOLATION OF Lactobacillus
FROM SAMPLE CULTURES.
• 3.DNA EXTRACTION USING THE MODIFIED SALTING–OUT METHOD.
• 4.IDENTIFICATION OF ISOLATES BASED ON 16S rDNA GENES
POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT-LENGTH
POLYMORPHISM AND SANGER SEQUENCING.
• 5.ADHESION OF Lactobacillus SPECIES TO S-HA.
• 6. ADHESION OF ISOLATED Lactobacillus SPECIES TO ORAL MUCOSAL
CELLS.
• 7.ANTIBACTERIAL ACTIVITY OF Lactobacillus SPECIES AGAINST ORAL
PATHOGENIC BACTERIA.
• 8.STATISTICAL ANALYSIS
 STUDY SUBJECTS
INCLUSION CRITERIA: EXCLUSION CRITERIA:

118 VOLUNTEERS DIVIDED INTO 2 History of smoking.

GROUPS: History of diabetes.

(AGE AND GENDER DISTRIBUTION; AGE Pregnancy

RANGE: 25 – 70 YEARS.) Breast Feeding

CONTROL GROUP Autoimmune disease

CASE GROUP
1. Periodontally healthy subjects. Necrotizing periodontal disease
1. MODERATE AND SEVERE 2. With at least 24 natural teeth
excluding third molars. Periodontal treatment in the past 6 months,
PERIODONTITIS.
2. PPD>3 mm ; CAL ; BOP ; 3. PPD ≤ 3 mm without predisposing receiving antibiotics or NSAIDs in the past 4
Bone Loss. factors causing local irritation or months, patients with prophylactic
plaque retention. antibiotics before routine dental treatments.
 RESULTS:
• ISOLATION AND IDENTIFICATION OF Lactobacillus
SPECIES.
ADHESION OF Lactobacillus SPECIES TO S- HA AND ORAL
MUCOSA CELLS.
ANTIBACTERIAL ACTIVITY OF ISOLATED Lactobacillus SPECIES
AGAINST TWO ORAL PATHOGENS.
 DISCUSSION:
• JOHNSTON et al., 2021; ALLAKER & STEPHEN ., 2017; MYNENI et al 2020 ; KURU et al
2017;
• Gupta et al 2011and Koll-Klais et al 2005; Ahrne et al 1998 ; Colloca et al 2000 ; Sornplang &
Piyadeatsoontorn ,2016.
• FAO/WHO, 2002 ; Colloca et al (Bosch et al);Terai et al 2015.
• Terai et al (2015)
• Ben Taheur et al 2016 ; Azizian et al 2021; Hirasawa and Kurita – Ochia ,2020 ; Rehne et al
2021; Sookkhee et al 2001; Koll-Klais et al 2005 ; Testa et al, 2003.
• Limitaion of this study. Case selection and availability of Pg.
• Zhang et al 2020 ; Hirasawa and Kurita – Ochia ,2020.
 CONCLUSION:
• The higher antibacterial properties of L.crispatus and
L.gasseri strains and the higher adherence ability of
L.salivarius and L.fermentum might be appropriate options
for probiotic treatment.
• Decreased frequency of lactobacillus strain indicates
1. Presence and function of pathogenic bacteria in
periodontal tissue.
2. Imbalance of oral microbial flora.
THANK YOU.