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  • Introduction To Cytogenetic FISH Techniques

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    This document provides an introduction to cytogenetic fluorescence in situ hybridization (FISH) techniques. It discusses how FISH utilizes fluorescent probes to detect specific DNA sequences within cells, allowing visualization and identification of genetic abnormalities. It explores applications of FISH in medical diagnosis, plant genetics, and ecological research. The document also covers advantages and limitations of FISH, as well as advancements like multiplex FISH and spectral imaging. Case studies demonstrate uses of FISH in identifying gene amplification in cancer, prenatal genetic screening, and species identification.

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    Introduction to Cytogenetic FISH Techniques

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    This document provides an introduction to cytogenetic fluorescence in situ hybridization (FISH) techniques. It discusses how FISH utilizes fluorescent probes to detect specific DNA sequences within cells, allowing visualization and identification of genetic abnormalities. It explores applications of FISH in medical diagnosis, plant genetics, and ecological research. The document also covers advantages and limitations of FISH, as well as advancements like multiplex FISH and spectral imaging. Case studies demonstrate uses of FISH in identifying gene amplification in cancer, prenatal genetic screening, and species identification.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    5 views7 pages

    Introduction To Cytogenetic FISH Techniques

    Uploaded by

    imanebourahmaoui
    This document provides an introduction to cytogenetic fluorescence in situ hybridization (FISH) techniques. It discusses how FISH utilizes fluorescent probes to detect specific DNA sequences within cells, allowing visualization and identification of genetic abnormalities. It explores applications of FISH in medical diagnosis, plant genetics, and ecological research. The document also covers advantages and limitations of FISH, as well as advancements like multiplex FISH and spectral imaging. Case studies demonstrate uses of FISH in identifying gene amplification in cancer, prenatal genetic screening, and species identification.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 7

    Introduction to

    Cytogenetic (FISH)
    Techniques
    Welcome to this presentation on cytogenetic (FISH) techniques. Discover how
    this powerful method helps study genetic abnormalities and offers new
    perspectives in the field of genetic research.

    FZ by Fatima Zahra EL ALAMI


    Understanding the FISH Technique

    1 Fluorescence In Situ
    Hybridization

    Learn about the fundamental principles


    Probe Hybridization 2 and underlying mechanism of FISH, a
    Delve into the process of probe molecular cytogenetic technique that
    hybridization, where fluorescently utilizes fluorescent probes to detect
    labeled DNA probes bind to specific DNA sequences within cells.
    complementary target sequences in the
    chromosomes, enabling visualization
    and identification. 3 Signal Detection

    Explore the methods used to detect and


    analyze fluorochrome signals resulting
    from the binding of probes to specific
    chromosomal regions, enabling the
    identification of genetic abnormalities.
    Applications of the FISH Technique

    Medical Diagnosis Plant Genetics Ecological Research

    Discover how FISH contributes Explore the application of FISH Uncover how FISH is employed
    to the accurate diagnosis and in plant genetics, allowing for in ecological research to assess
    classification of genetic the identification and analysis biodiversity, population
    disorders, aiding in personalized of specific chromosomal genetics, and environmental
    medicine and improving patient regions, contributing to crop impact studies, providing
    care. improvement and genetic insights into the health of
    engineering. aquatic ecosystems.
    Advantages and Limitations of FISH

    1 Precision and 2 Limitations and 3 Advancements and


    Sensitivity Challenges Future Potential

    Learn about the high Understand the


    precision and sensitivity challenges associated Explore the latest
    of FISH, allowing for with FISH, including advancements in FISH
    the detection of specific potential false-positive technology, such as
    DNA sequences even at or false-negative results, multiplex FISH and
    low concentrations. tissue-specific spectral imaging, and the
    limitations, and the need potential future
    for skilled interpretation applications of this
    of data. technique in clinical and
    research settings.
    Exciting Research Areas in Cytogenetics

    Cancer Genomics Developmental Evolutionary Biology


    Disorders
    Discover how cytogenetic Uncover how cytogenetic
    techniques, including Explore how cytogenetics, techniques are used to
    FISH, contribute to alongside other genetic unravel the evolutionary
    understanding the genomic diagnostic methods, aids in history of different species,
    alterations underlying elucidating the genetic shedding light on genomic
    various types of cancer, causes of developmental rearrangements, speciation
    enabling targeted therapies disorders, providing vital events, and the
    and personalized medicine. insights into prenatal and diversification of life
    pediatric clinical practice. forms.
    Case Studies Using FISH Technique
    1 Gene Amplification in Cancer

    Explore a compelling case study where FISH identified gene amplification (e.g., HER2) in
    breast cancer, leading to targeted therapy and improved patient outcomes.

    2 Prenatal Genetic Screening

    Discover the application of FISH in prenatal genetic screening, enabling the non-invasive
    detection of chromosomal abnormalities, such as trisomy 21 (Down syndrome), providing
    crucial information for parents-to-be.

    3 Species Identification

    Learn about a fascinating case study that employed FISH techniques to differentiate and
    identify closely related species, contributing to conservation efforts and understanding
    biodiversity.
    Conclusion and Future Perspectives
    Summarize the significance of the FISH technique in cytogenetics, highlighting its contributions to medical
    diagnosis, research advancements, and evolutionary studies. Discuss potential future developments and
    emerging applications in the field.

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