FIRST REPORT OF ANAPLASMA MARGINALE

INFECTION IN GOATS, BRAZIL

Published in
Authors PLOS one
da Silva et al (2018) (W category, 3.75 IF)

Presented By
Farwa Malik
 About the Paper

Type of the Paper

Experimental Case Study Chi-square or the Fisher’s

exact test applied to test
Area of Study
Association of factors
Brazil (age, gender, presence of
ticks, and multispecies Results
grazing)
Sample of Study
With
403 Goats Anaplasma infection.

Data Collection Method

Questionnaire
Introduction to Anaplasma Marginale

causative agent of bovine anaplasmosis,

a tick-borne bacterium

causes significant economic losses

for cattle industries

increasingly detected in other

animals

Rhipicephalus microplus is main vector

 Introduction-Continued

Found where Ticks are endemic

Tick species are found

parasiting small ruminants

In Brazil, R. Microplus is endemic

A. Marginale strain co-grazing

animals cow, buffaloes & Goats

A.Marginale is not found in goats in Brazil

 Materials and Methods

 a total of 403 blood samples from goats (368

females and 35 males),
 Samples were stored at -80 C in anticoagulant
tube containing ethyl-enediaminetetraacetic acid

 Genomic DNA was extracted from 200 μL of

whole blood using a commercial kit (GE
Healthcare, Little Chalfont, UK)
 PCR amplification of the caprine
glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) housekeeping gene to verify successful
DNA extraction

 Samples were screened for A. marginale- and A.

ovis-infection
 identity of A. marginale in the blood was
confirmed by PCR amplification of msp5
 Results
All samples have successfully amplified the GAPDH gene

Eleven out of 403 goats (2.73%; CI 95%: 1.53–4.82%) were

positive for the Anaplasma msp4 gene

Ten out of Eleven samples were positive for Anaplasman msp5

gene through CPR

sequencing of the msp5 gene confirmed the presence of A.

marginale

Tick-infested goats were six times more likely to be infected with

A. marginale (p = 0.02788)

The tick species feeding on the goats were identified as

Amblyomma parvum (94.23%) and R. microplus (5.77%)
Results-Continued
 Discussion & future work

1. Ticks feeding on goats and cattle may transfer the pathogen between the
two livestock species during co-grazing

2. Anti-Anaplasma spp. antibodies have been identified in goats from

northeastern Brazil

3. Direct molecular detection of A. marginale in small ruminants has

been only reported in sheep from Iran

4. Previous studies have suggested that Amblyomma ticks may be

involved in the transmission of A. marginale

5. studies are necessary to evaluate if A. parvum is competent vector of f A.

marginale