Charm Quantitative Milk

Aflatoxin Test

MRLAFMQ Test
What is Aflatoxin?

• Aflatoxin is a natural toxin, and potent

carcinogen, produced by the mold Aspergillus
flavis and Aspergillus parasiticus

• Aflatoxin is a broader group of mold

produced toxins called mycotoxins.

• Mold growth reduces crop yields and

livestock productivity

• Mold growth can occur pre-harvest and post

harvest

• There are 4 Aflatoxins produced by mold

growth in grains: B1, B2, G1, G2. B1 is the
predominate form 80%-95%

• Aflatoxin B1 when ingested by dairy cows is

converted to Aflatoxin M1 & M2. M1 is the
predominate Aflatoxin found in milk
Corn exposed to
moisture for 18 days

Properly stored corn

Action Levels of Aflatoxins (MRL)

Must Not Exceed Description

0.05 ppb (50 ppt) Milk (Aflatoxin M1)
Introducing the Charm® MRL Aflatoxin
Milk Test
Equipment needed to run MRLAFMQ ?

• ROSA® Reader with 3-line

aflatoxin calibration,
version 1.09 or higher. (The Charm ROSA
3 Line AFLA Capable
green label on the bottom
of the Rosa Reader
indicates it is compatible
with the 3 line aflatoxin
test.)
Or, ROSA Pearl Reader with
green label on unit base
AFLA Channel, mode 3
• Quad or Dual 40oC
15 minute Incubator Quad 40oC 15-min incubator

ROSA Pearl Reader

MRLAFMQ Storage Specifications

• Store MRLAFMQ Test strips,

Aflatoxin M1 Milk Standard
refrigerated: 0 to 7°C
• Remove from container the
number of test strips to be
used in one day. Keep these
test strips at room
temperature during daily use
for up to 12 hours. Unused
test strips should be discarded
• Use supplied plastic lid to
immediately reseal container
tightly and return to
refrigerated storage
MRLAFMQ Negative Control

• Use aflatoxin negative

raw commingled milk as a
Negative Control.
• Negative Control may be
stored up to 72 hours
refrigerated (-15°C or
below for up to 2
months)
• Negative Control must be
RESULT NEGATIVE with
READING = 0 on ROSA
Reader
MRLAFMQ Test Procedure
Do not Read “Invalid
Strips” where the “C”
appears like:
Concentration-Response of
MRLAFMQ method

Concentration % Positive†
(ppt*) on Rosa Reader
0 0%

25 0%
50† 100 %
75 100 %

*parts per trillion or ng/kg

†90% positive with 95% confidence
Calibration Strips for Standard
ROSA Readers –3 line for MRLAFMQ

• Calibrator serial #s match

ROSA Reader serial #
• Read in Sample 3-Line
Mode, AFLA Channel
• Results for NEG & POS
Calibration Strips must
read within White
Labeled Range
• Do not proceed…”
if out of range”
ROSA PEARL Calibration Strips:
MRLAFMQ
• Can be read on any channel
• For ROSA Pearl Reader only
• One set for all ROSA tests
• Can read either side
• Can ONLY be read under
Performance Mode “ESC 5”, 3-
Line Mode
• Calibrator serial #s match Pearl
Reader serial #
• Do not proceed…“if out of range”
 Questions?

© 2011-2012 Charm Sciences, Inc. All rights reserved. Charm, and ROSA names, are registered trademarks of
Charm Sciences, Inc. MRK-604-001 0212