MICROBIOLOGY

QUESTION AND ANSWER SESSION

 1
Bacteria are
a)Prokaryotic
b) Eukaryotic
c) Animal
d) Protozoa
e) Helminthes
 2
• One is not a Romanowsky stain:
• a) Fields stain
• b) Gram stain
• c) Geimsa stain
• d) Leishman stain
 3
• Romanowsky stain consists of:
• a) Eosin + Alkaline methylene blue
• b) Eosin only
• c) Methylene blue only
• d) Indian Ink
 4
• Difference between gram Positive and gram
negative bacteria is
• a) Cell membrane
• b) Cell wall
• c) Nucleus
• d) Cytoplasm
 5
• Primary stain of gram stain:-
• a) Crystal violet
• b) Iodine
• c) Safranine
• d) Malachite green
 6
• Secondary stain of gram stain:-
• a) Crystal violet
• b) Iodine
• c) Safranine
• d) Malachite green
 7
• All the following are used in gram stain except:
• a) Malachite green
• b) Neutral red
• c) Methyl violet
• d) Iodine
• e) Ethyl alcohol
 8
• Gram negative bacteria color appear :
• a) Dark purple
• b) Pale to dark red
• c) Orange
• d) Blue
 9
• Gram Positive bacteria color appear :
• a) Yellow
• b) Pale to dark red
• c) Orange
• d) violet
• e) Black
 10
• In gram positive bacteria there is.
• a) Peptidoglycan and teichoic acid.
• b) Has no peptidoglycan.
• c) Has no teichoic acid.
• d) None of above
 11
• -In the lab gram stain use with the following
except.
• a) Bacteria smear
• b) Pus cell smear.
• c) Urethral discharge.
• d) C.S.F
• e) Water culture
 12
• In gram stain, which of these is used as a basic
Dye:
• a) Methyl violet / Crystal violet
• b) Safranin /Neutral red
• c) Gram iodine
• d) Acetone / alcohol
 13
• On gram staining one of the following bacteria
form chain
• a) Streptococci
• b) Staphylococci
• c) Bacillus
• d) Mycobacterium
• e) Diplococci
 14
• Gram +ve cocci arranged in groups (clusters)
• a) Streptococci
• b) Staphylococci
• c) Cocci
• d) spiral
 15
• Which of the following microscope would you
use to examine a smear of bacteria stained by
gram stain:-
• a) Electron microscope
• b) Dark field microscope
• c) Fluorescent microscope
• d) Bright field microscope
 16
• Gram negative bacterial cell wall characterized
by the presence of
• a) Large amount of Glucose.
• b) Large amount of glycogen
• c) Large amount of Cellulose
• d) Toxic lipolysaccharide molecules.
 17
• Ziehl nelson stains used in?
• a) Bacillus.sp
• b) Mycobacterium tuberculosis
• c) H.influenza.
• d) None of above
 18
• Mycobacterium tuberculosis is called acid-fast
because:-
• a) It is easily stained by gram stain
• b) Once stained, it can not be removed by acid
• c) It is easily decolorized after staining.
• d) It is stained by an alkaline stain
• e) None of the above
• f) All of the above
 19
• All are use in Zeihl Nelson stain except.
• a) Carbol Fuchcin.
• b) Acid alcohol.
• c) Crystal violet.
• d) Malachite green
 20
• Albert's stain use with which of the organism.
• a) E.coli.
• b) H. influenza.
• c) C.tetani.
• d) Corynebacterium diphtheria.
 21
• Stain required to demonstrate bacterial
capsules
• a) Gram stain
• b) Giemsa stain
• c) Leishman stain
• d) India Ink stain
 22
• Which of the following culture media is
suitable for semi quantitative bacterial count
in urine samples :-
• a) MaCconkey agar
• b) Blood agar
• c) XLD medium
• d) Mannitol salt agar
• e) CLED medium
 23
• CLED agar use in urine culture because it?
• a) It is transport media.
• b) It is basic media.
• c) Help in colonies count and prevent
swarming of proteus .
• d) All above.
 24
• Thayer-martin media is the choice for the
isolation of the following organism:
• a) Pseudomonas aeroginosa
• b) Haemophilus influenzae
• c) Nisseria gonorrhoea
 25
• Media use for tuberculosis:
• a) Lowenstein Jensen media
• b) Nutrient agar
• c) Urease broth
• d) Sabarouds dextrose media
 26
• Bordet gengou medium is best for isolation of
• a) Bordetella pertusis
• b) Moraxella catarrhalis
• c) Haemophilus influenzae
• d) S.pyogenes
 27
• Bacteria that can grow in presence or absence
of O2 is called?
• A) Facultative anaerobic
• B) Strict anaerobic
• C) Anaerobic
• D) None of above.
 28
• Optimum temperature for growth of
pathogenic bacteria is?
• a) 50C.
• b) 60C.
• c) 37C.
• d) None of above.
 29
• Which test identifying staphylococcus from
streptococcus it is.
• a) Catalase.
• b) Coagulase.
• c) Oxidase.
• d) Urease
 30
• Bacterial cell contain all the following except:
a) Ribosome
• b) Plasmid
• c) Mitochondria
• d) Cell membrane
• e) Pili
 31
• Media for detection of hemolysis is.
• a) Blood agar.
• b) Nutrient agar.
• c) MacConkey.
• d) All above
 32
• Most common bacteria that produce urease are.
• a) Proteus.
• b) Pseudomonas.
• c) E.coli.
• d) None of above.

• PUNCH- Protues Ureaplasma Nocardia

Cryptococcus Helicobacter
 33
• One is always oxidase +ve
• a) Haemophilus Influenza
• b) Pseudomonas aerugenosa and Neisseria
• c) E.coli
• d) Yersinia
 34
• Reagent used in catalase reaction:
• a) H2O2
• b) CO2
• c) H2O
• d) HCO3
 35
• Stage in which bacteria grow and multiply
called
• a) Lag phase
• b) Log phase
• c) Stationary phase
• d) Decline phase
 36
• Stage in which bacteria make adaptation in
media called
• a) Lag phase
• b) Log phase
• c) Stationary phase
• d) Decline phase
 46
• Which of the following stuff can not be
sterilized in hot oven
• a) Glass or aluminum Petri dishes
• b) Glass tubes
• c) Glass flask and cylinders
• d) Metal needles and glass pipettes
• e) Culture media
 47
• The sterilization in hot air oven performed at:
• a) 121 c° for 15-30 min.
• b) 160 c° for 45-60 min.
• c) 121 c° for 10 min.
• d) 160 c° for 10 min.
 48
• The sterilization in autoclave performed at:
• a) 121 c° for 15-30 min.
• b) 160 c° for 45-60 min.
• c) 121 c° for 10 min.
• d) 160 c° for 10 min.
 49
• - Bacteria can cause pathogenesis to human
by:
• a) Capsule
• b) Secret enzymes
• c) Endogenous toxins
• d) Exogenous toxins
• e) All of the above
 50
• Function of pili of the bacteria:
• a) Attachment to the host tissue
• b) Movement
• c) Reproduction (multiplication)
• d) Engulf of food
• e) All of the above
 65
• Bacteria that need dark field microscope to
detecte
• a) T. pallidium
• b) Klebsiella
• c) Streptococcus pyogenes
• d) Clostridia sp
 66
• Growth of vibrio cholerae on TCBS medium
produces colonies
• a) Yellow colonies
• b) Blue colonies
• c) Green colonies
• d) White colonies
• e) Red colonies
 67
• Media use for differentiate between L.F and
N.L.F:
• a) MacConkey agar
• b) Chocolate
• c) Blood agar
• d) XLD
• e) TCBS
 68
• All of the following are true about agar
except:-
• a) agar is inert(Non nutritive)
• b) Nutritious
• c) Solidity at 42c
• d) Melts at 100c
 69
• Which of the following are sterilized in the
oven:-
• a) Media
• b) Sand
• c) Water
• d) Blood
• e) All of the above
 70
• To obtain an isotonic media, sodium chloride
content should be:-
• a) 0.58 g %
• b) 0.085 g%
• c) 8.5 g%
• d) 6.5 g%
• e) 0.50 g%
 72
• Micro-organisms that grow at 60 c are
classified as:-
• a) Psychophysics
• b) Mesosphilic
• c) Hemophilic
• d) Thermophilic
• e) Saprophilic
 77
• -For blood culture the best anticoagulant is
• a) Citrate
• b) Oxalate
• c) Sodium polyanethol sulphonate(SPS)
• d) Heparin
• e) EDTA
 81
• A standard micro plates in ELISA test has?
• a) 98 wells
• b) 94 wells
• c) 96 wells
• d) 48 wells
 82
• The immunoglobulin with smallest molecular
weight is
• A) IgG
• B) IgA
• C) IgM
• D) IgE
• E) IgD
 83
• The immunoglobulin Which is present in trace
amount is
• A) IgG
• B) IgA
• C) IgM
• D) IgE
• E) IgD
 84
• Motile bacteria have
• A) H Ag
• B) F Ag
• C) Vi Ag
• D) O Ag
 85
• Porosity of membrane filter used for serum
sterilization
• A) 0.22 Micrometre
• A) 2.2 Micrometre
• A) 0.6 Micrometre
• A) 0.8 Micrometre
 86
• In significance bacteria cfu/ml is.
• A) > 100.
• B) >1000.
• C) >10000.
• D) 100000
 87
• One of the following is an example for
enrichment media:
• A) Blood agar.
• B) CLED.
• C) Nutrient agar.
• D) Selenite broth.
 88
• - Enriched media are required for the culture
of
• A- hemophilus influenza
• B- Neisseria
• C- streptococcus
• D- All of them
 89
• These are media to which indicators or dyes
are added to differentiate microorganisms as
XLD agar
• A- Basic
• B- Enriched
• C- Selective
• D- Differential
 91
• Decontaminate bench surface are sterilized by
• A- 1h in 2500 ppm chlorine
• B- 5 % phenol
• C- both A,B is true
• D- 1h in 1000 ppm chlorine
 92
• Loffler’s serum is used for isolation of:
• a- anthrax
• b- clostridium
• c- diphtheria
 94
• - the Kovac's reagent used in the following
biochemical tests:
• a- catalase
• b- coagulase
• c- indole
• d- methyl red
 95
• - positive results for H2S production appear as
…. Colour:
• a- black
• b- yellow
• c- red
• d- green